• Title/Summary/Keyword: Isolation number

Search Result 527, Processing Time 0.03 seconds

Studies on the Efficient Separation of Primordial Germ Cells and Introduction of Foreign DNA in the Chicken (닭에서 원시생식세포의 효율적 분리 및 외래 유전자 전이에 관한연구)

  • 정동기;한재용
    • Proceedings of the Korea Society of Poultry Science Conference
    • /
    • 1999.11a
    • /
    • pp.11-33
    • /
    • 1999
  • This study was conducted to determine the embryonic stages for the isolation of the highest number of PGCs and to improve PGCs enrichment method. The primordial germ cells(PGCs) from different sources of chick embryos were isolated. The embryonic stage having the highest number of PGCs from each sources was selected ; 1-day-old embryos for germinal crescent (stage 6-8), 2.5-day-old embryos for blood (stage 17-18) and 5.5-day-old embryos for gonad (stage 27-28). The number of PGCs from one embryonic germinal crescent, blood and gonad was about 87$\pm$1.8, 103$\pm$4.0, and 932$\pm$10.9, respectively. The viability of PGCs after Ficoll from each sources was similar, showing approximately 70%. the PGCs enrichment method was improved using Ficoll density gradient centrifugation. After this step the purity of PGCs from germinal crescent, blood, and gonad was 45$\pm$9.10%, 85$\pm$1.18%, and 86$\pm$0.19%, respectively. Also, PGCs were picked up by mouth pipette to improve the purity. This improved method for the separation of PGCs from different sources will serve as a useful too to preserve the foundation stocks of poultry and to produce germline chimeras.

  • PDF

Implementation of A Hospital Information System in Ubiquitous and Mobile Environment

  • Jang, Jae-Hyuk;Sim, Gab-Sig
    • Journal of the Korea Society of Computer and Information
    • /
    • v.20 no.12
    • /
    • pp.53-59
    • /
    • 2015
  • In this paper, we developed a Hospital Information System in which the business process is formalized and a wire/wireless integrated solution is used. This system consists of the administration office program, the medical office program, the ward management program and the rounds management program. The administration office program can enroll and accept patients, issue and reissue the RFID card. The medical office program inputs a medical examination and treatment, outputs a diagnosis, requests a hospitalization, retrieves the record of a medical examination and treatment, assigns the corresponding examination room to the accepted patients, and updates the number of an waiting patient and a patient number according to the examination room on real time. The ward management program handles hospitalizations and leaving hospital, a nurse's note, and an isolation ward monitoring. The rounds management program handles a medical examination and treatment, and a leaving hospital using PDA. This developed system can be built at low cost and increase the quality of the medical services highly by making it automated the medical administration automation. Also the small number of the medical staffs can manage the inpatients efficiently by using the monitoring functions.

Karyotype Analysis of an Endemic Korean Torrent Catfish Liobagrus hyeongsanensis(Siluriformes: Amblycipitidae) (한국 퉁가리속 고유종 동방자가사리 Liobagrus hyeongsanensis의 핵형 분석)

  • Cho, Yun Jeong;Park, Jong Young
    • Korean Journal of Ichthyology
    • /
    • v.29 no.2
    • /
    • pp.89-93
    • /
    • 2017
  • For the karyotype analysis of Liobagrus hyeongsanensis, an endemic Korean torrent catfish, ten females and five males were used from Yangbuk-myeon, Gyeongju-si, Gyeongsangbukdo, Korea. The diploid number of chromosomes was 42, and its karyotype consisted of 30 metacentrics and 12 submetacentrics; 84 FN (fundamental number), with having no polyploidy and sexual dimorphism. The chromosome number was the same as other closely-related species, L. mediadiposalis and L. somjinensis, but their karyotypes showed a clear difference by species. Such result may be related to chromosomal rearrangements by Robertsonian rearrangement with geographical isolation.

Water Distribution Network Partitioning Based on Community Detection Algorithm and Multiple-Criteria Decision Analysis

  • Bui, Xuan-Khoa;Kang, Doosun
    • Proceedings of the Korea Water Resources Association Conference
    • /
    • 2020.06a
    • /
    • pp.115-115
    • /
    • 2020
  • Water network partitioning (WNP) is an initiative technique to divide the original water distribution network (WDN) into several sub-networks with only sparse connections between them called, District Metered Areas (DMAs). Operating and managing (O&M) WDN through DMAs is bringing many advantages, such as quantification and detection of water leakage, uniform pressure management, isolation from chemical contamination. The research of WNP recently has been highlighted by applying different methods for dividing a network into a specified number of DMAs. However, it is an open question on how to determine the optimal number of DMAs for a given network. In this study, we present a method to divide an original WDN into DMAs (called Clustering) based on community structure algorithm for auto-creation of suitable DMAs. To that aim, many hydraulic properties are taken into consideration to form the appropriate DMAs, in which each DMA is controlled as uniform as possible in terms of pressure, elevation, and water demand. In a second phase, called Sectorization, the flow meters and control valves are optimally placed to divide the DMAs, while minimizing the pressure reduction. To comprehensively evaluate the WNP performance and determine optimal number of DMAs for given WDN, we apply the framework of multiple-criteria decision analysis. The proposed method is demonstrated using a real-life benchmark network and obtained permissible results. The approach is a decision-support scheme for water utilities to make optimal decisions when designing the DMAs of their WDNs.

  • PDF

A Study on Microbial Pollution of Indoor Air at Elderly Care Facilities (노인요양시설의 실내공기 중 미생물 오염에 관한 연구)

  • Kim, Sang-Ha;Kim, Young-Kwon
    • Journal of the Korea Academia-Industrial cooperation Society
    • /
    • v.10 no.9
    • /
    • pp.2485-2491
    • /
    • 2009
  • Culture was performed by using Sheep Blood Agar Plate (BAP, Asan Pharmaceutical) and Sabouraud Dextrose Ager (SDA, Asan Pharmaceutical) along with air $IDEAL^{TM}$ (Biomerieux), which is a microbe interceptor based on inertial impaction interception, in order to investigate bioaerosol in indoor and outdoor air at five elderly care facilities in a metropolis and an urban-rural consolidated city for two months from April 1 to May 31, 2007. From the culture followed by isolation and identification, the following conclusions were drawn. 1. As for the general isolation of microbes in each facility, care center S had the largest amount of microbes (263 cfu/$m^3$) isolated in a 300L room, followed by care center U having 123 cfu/$m^3$ isolated. 2. As for the number of bacteria isolated from a medium intercepting 300 L indoor, the largest amount of other unidentified or non-pathogenic Gram positive cocci (321 cfu/$m^3$) was isolated and most of the other Gram positive cocci were CNS (Coagulase Negative Staphylococcus). 3. As for the number of fungi isolated from a medium intercepting 300 L in a room, the largest number of Aspergillus spp. (66) was isolated, followed by Mucor spp. (62 cfu/$m^3$), Penicillium spp. (53 cfu/$m^3$), Alternaria spp. (50), and other unidentified or non-pathogenic fungi (42 cfu/$m^3$). 4. As for the rate of indoor and outdoor pollution, the average number of interceptions was all larger indoor than outdoor; the research differentiating the amount of air into 300 L and 500 L demonstrated that the larger amount of air led to more bacteria, making no great variation in the species.

Cellular Slime Molds in Forest Soils of Central Areas of Korea (중남부 삼림 지역에서의 세포성 점균의 출현과 분포)

  • 박미아;장남기
    • Asian Journal of Turfgrass Science
    • /
    • v.10 no.3
    • /
    • pp.213-230
    • /
    • 1996
  • A study of occurrence and distribution of cellular slime molds(CSMs) in forest soils of central areas of Korea was carried out. Samples for CSMs isolation were collected from 4 study sites; Mt.Kyeryong, Mt. Taebaek, Mt. Sobaek and Mt. Sokri. In Mt. Kyeryong, six species were found. These were Dictyostelium fasciculatum, D.firmibasis, D. mucoroides, D. minutum, D. brefeldianum and Polysphondylium pallidum. The average number of species isolated at one site was 0.75, and average density(clones /g soil) was 292. The results of soil sample analyses were that the concentration of Pb was higher than any other areas. In Mt. Taebaek, seven species were found; D. fasciculatum, D.firmibasis, D. mucoroides, D. miuutum, P.pallidum. P. violaceum, P. candidum. The average number of species isolated at one site was 2.3,and average density was 1,108. Based on importance values calculated from study sites within each of three elevation ranges, several of the more widely distributed and abundant species have distribution patterns that show a response to elevation. In Mt. Sobaek, eleven species were found. These were D. fasciculatum. D.firmibasis, D. mucoroides, D. miuutum, D. brefeldianum, D. crassicaule,D. deminutivum, D. implicatum, P. pallidum. P. violaceum and P. candidum. The average number of species isolated at one site was 3, and average density was 793. Species diversity appeared to be the highest in this area. In Mt. Sokri, six species were found. These were D. fasciculatum. D. mucoroides, D. minutum, D. purpureum, P. pallidum. and P. violaceum. The average number of species isolated at one site was 2.4 and average density was 858. It was noticeable that D.purpureum were much more comrnonly found in this pinus evergreen forest. Key words: Cellular slime molds(CSMs), Importance value, Elevation, Average number of species, Average density.

  • PDF

Minimum Number of Input Ground-motions to Assess Seismic Performance of Nuclear Facilities (원전시설의 내진성능평가를 위한 입력지반운동의 최소개수)

  • Hong, Kee-Jeung;Choi, Ji-Hae;Kim, Hyun-Uk;Joo, Kwang-Ho
    • Journal of the Earthquake Engineering Society of Korea
    • /
    • v.20 no.5
    • /
    • pp.341-349
    • /
    • 2016
  • Currently, researches are being actively conducted in assessing seismic performance of nuclear facilities in USA and Europe. In particular, applying this technique of assessing seismic performance to design of isolation systems in nuclear power plants is being performed and then ASCE 4 Draft (2013) is being revised accordingly in the United States. In order to satisfy the probabilistic performance objectives described by seismic responses with certain confidence levels (ASCE 43, 2005), the probability distributions of these responses have to be defined. What is the minimum number of input ground-motions to obtain the probability distribution precise enough to represent the unknown actual distribution? Theoretical basis, for how to determine the minimum number of input ground-motions for given a logarithmic standard deviation to approximate the unknown actual median of the log-normal distribution within a range of error at a certain level of confidence, is introduced by Huang et al. (2008). However, the relationship between the level of confidence and the range of error is not stated in the previous study. In this paper, based on careful reviews on the previous work, the relationship between the level of confidence and the range of error is logically and explicitly stated. Furthermore, this relationship is also applied to derive the minimum number of input ground-motions in order to approximate the unknown actual logarithmic standard deviation. Several recommendations are made for determining the minimum number of input ground-motions in probabilistic assessment on seismic performance of facilities in nuclear power plants.

Factors Predicting Early Release of Thyroid Cancer Patients from the Isolation Room after Radioiodine-131 Treatment

  • Fatima, Nosheen;Zaman, Maseeh uz;Zaman, Areeba;Zaman, Unaiza;Tahseen, Rabia;Shahid, Wajiha
    • Asian Pacific Journal of Cancer Prevention
    • /
    • v.17 no.1
    • /
    • pp.125-129
    • /
    • 2016
  • Background: Patients with differentiated thyroid cancers (DTC) who receive radioactive iodine-131 (RAI) are released from isolation when their dose rate is below the regulatory requirements. The purpose of this study was establish predicting factors for early release from the isolation facility after RAI administration in patients with DTC. Materials and Methods: This was a prospective study which included 96 (58 females and 38 males) patients with DTC who had received RAI from April 2013 till August 2015. The study was duly approved by the ethical committee of the institute. Patients who had complete information of primary tumor size (PTS), serum TSH, stimulated thyroglobulin level [sTg] with antibodies (IU/ml) at the time of RAI treatment were included. All had a normal serum creatinine level. To attain lower effective half-life good hydration and administration of soft laxative were ensured. Dose rate was measured (immediately, 24 h and 36 h) at 1 meter distance from anterior mid trunk and a dose rate <$50{\mu}Sv/h$ was considered as the releasing criterion. At 24 h 50 patients were released while the remaining 46 patients were released at 36 h. A post-ablative whole body scan (PA-WBIS) was performed 5-8 days after RAI ablation in all patients. Results: Patients released after 24 h were significantly younger, had smaller lesions with higher proportion of papillary cancer, lower sTg, lower sTg/TSH ratio and had received a lower dose of RAI as comapred to those who were discharged after 36 h. Serum TSH and gender were not found to have any significant correlation between two cohorts. ROC and multivariate analysis have shown age ${\leq}37years$, PTS ${\leq}3.8cm$, $RAI{\leq}150mCi$, $sTg{\leq}145ng/ml$ and $sTg/TSH{\leq}1.085$ as strong indepedent predictors for early release. Conclusions: We conclude that younger age (${\leq}37years$), smaller tumor size (${\leq}3.8cm$), lower RAI dose (${\leq}150mCi$), lower sTg (${\leq}145ng/ml$) and a lower sTg/TSH ratio (${\leq}1.085$) are significant independent predictors for release at 24 h after RAI treatment in DTC patients. Effective utilization of these factors could help the treating physicians to use limited number of internment facilities with higher throughput, lower cost and lower psychological stress to patients.

Influence of Discrimination Experience in Daily Life and Social Isolation on Depression of Older Adults (노인의 일상생활에서의 차별 경험과 사회적 고립이 우울에 미치는 영향)

  • Ko, Young;Kwak, Chanyeong
    • Journal of Convergence for Information Technology
    • /
    • v.11 no.8
    • /
    • pp.42-52
    • /
    • 2021
  • This study was conducted to identify the influence of the discrimination experienced in daily life and social isolation on depression among older adults living in the community. This study was a secondary analysis of the data of 2017 Living Profiles of Older Adults Survey. The participants was a representative sample among the older adults 65 years and older. Data from 10,041 older adults were analyzed for this study. Hierarchical logistic regression analyses were used. When the discrimination experiences was added in model 1, the likelihood of being depressed was 1.95(1.60-2.36) times higher for those who experienced discrimination comparing with those who didn't experienced discrimination. When the social isolation was added in model 2, the likelihood of being depressed was 1.89(1.55-2.30) times higher in those who experienced discrimination. In addition, as the number of close friends, neighbors, and acquaintances decreased by one, the likelihood of being depressed increased by 1.14 times. Those who were isolated from family, friends, neighbors and acquaintances were 3.90 times more likely to be depressed. Therefore, social efforts are needed to reduce the experience of discrimination. Maintaining a social network or creating a new network will contribute to lowering the level of depression in older adults who have experienced discrimination.

EVALUATION OF PIT AND FISSURE SEALANTS RETENTION BETWEEN DIFFERENT OPERATORS USING DIFFERENT ISOLATION TECHNIQUES (시술자와 방습법에 따른 치면열구전색제의 유지에 관한 연구)

  • Cho, Jae-Yong;Kim, Jung-Wook;Jang, Ki-Taeg;Kim, Chong-Chul;Hahn, Se-Hyun;Lee, Sang-Hoon
    • Journal of the korean academy of Pediatric Dentistry
    • /
    • v.30 no.3
    • /
    • pp.415-422
    • /
    • 2003
  • Currently, 'National program of dental sealant' has begun in Korea, but various isolation techniques and preparation is performing. The aim of the present study was to compare pit and fissure sealant retention rates between different operators using two different isolation techniques. Additionally, the failed surface was examined. One examiner performed the examination after one year of the initial operation. The population consisted of 119 children(mean age $8.59{\pm}0.772$) of which the total sealed number was 279 permanent first molars. Pediatric dentists sealed 131 molars using the rubber dam isolation technique (Group 1), general practitioners sealed 80 molars using the rubber dam isolation technique (Group 2), and dental hygienists sealed 69 molars using the cotton roll isolation (Group 3). The results were as follows ; 1. The complete retention rate between Group 1 (90.8%) and 2 (85.0%) showed no significant statistical difference (P>0.05). However, there were significant statistical different retention rates between Group 1 and Group 3(64.7%) and between Group 2 and Group 3 (p<0.05). 2. In comparing retention rates between maxilla and mandible, Only Group 3 showed a significantly lower complete retention rate in mandible than maxilla (p<0.05). 3. In failed surface analysis, occlusal failed surface was 58.3%, buccal/palatal failed surface was 41.7%.

  • PDF