• 한국생물공학회:학술대회논문집
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• 2003.04a
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• pp.409-411
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• 2003

A species of facultative photo-organotrophic, purple, non-sulfur bacterium was isolated from the west coast and the south coast 47 area of Korea at 2001 September. Separated 13 samples of changes with red color under $28{\sim}32\;^{\circ}C$, 3000 lux, anaerobe conditions for 7 days cultivated in Basal medium. For a pure isolation from 13 samples it used agar-shake tube method (0.4 % agar) and it separated 5 strains through 13-repetition test. The RAPD(Random Amplified Polymorphic DNA)-PCR result of strains (EGH-9, EGH-13, EGH-23, EGH-24, EGH-30) that EGH-24 and EGH-30 was same strain. For wastewater biodegradation test that 4 isolation strains cultivated in synthesis wastewater in 7 days. EGH-24 was high 63000 mg/L (CODcr) to 43400 mg/L (CODcr). EGH-24 was selected with efficient wast water treated strain. Based on the results obtained from morphology, nutrient requirements, major bacteriochlorophyll content, 165-rDNA phylogenetic analysis, this strain may be identified as a new strain of the genus Rhodobacter and named Rhodobacter sp. EGH-24.

• The Journal of Korean Society of Virology
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• v.29 no.2
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• pp.65-74
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• 1999

Reovirus was found to inhabit both the respiratory and the enteric tract of human and animals. The genome of reovirus comprises 10 segments of double-stranded RNA, total size 24 kbp. Nine strains of reovirus were isolated from human and field mice in Korea. Aseptically collected sera from human and lung tissues from field mice were used for virus isolation. For serotype determination, hemagglutination inhibition test was used, and three strains were confirmed to type 2 and six strains to type 3. To determine the genomic diversity and molecular phylogeny of reoviruses isolated in Korea, part of S4 genomic segment of reovirus was enzymatically amplified and directly sequenced. In nucleotide level, Apo98-35 strain showed 15.4%, 19.3%, and 14.4% differences compared to type 1 (T1L, Lang), type 2 (T2J), and type 3 reference strains, respectively. In amino acid level, Apo98-35 strain showed 10.5%, 13.7%, and 9.5% differences compared to type 1, type 2, and type 3 reference strains, respectively. Using the maximum parsimony method based on 285 bp spaning region of the S4 genomic segment, phylogenetic analysis indicated that Apo98-35 from Korea formed different phylogenetic branch. Our data obtained by sequence and phylogenetic analyses of reoviruses are consistent with the distinct geographically dependent evolution of reoviruses in Korea.

• Korean Journal of Veterinary Research
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• v.40 no.1
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• pp.42-48
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• 2000

Akabane disease is transmitted through mosquitoes in cattle, sheep and goats. It shows congenital abnormalities including encephalomyetitis, hydranencephaly, neurogenic arthrogryposis, and deformed neonatal calves. Akabane viruses, 93FMX and K-9 strain, were isolated from fetal matrix of aborted cow and blood of healthy cow, respectively. S gene sequences of 93FMX and K-9 showed 100% homology with that of OBE-1 strain isolated in Japan. Based upon our sequencing data, we synthesized specific primers for PCR diagnosis. Using these primers, we were able to amplify the S gene of Akabane virus not only from the culture fluid of Vero cells but also from the brain tissue of suckling mouse inoculated with, Akabane virus. These PCR products were confirmed by Southern blot hybridization. Not only the sensitivity of PCR test was high enough to detect the viruses of $10^{1.0}TCID_{50}/ml$, but also the time for diagnosis was significantly shorter than that of the virus isolation by tissue culture method. This method was also effective for the detection of Akabane virus in the cerebrum of fetus. RT-PCR method may be used for a useful diagnostic test of the clinical cases of Akabane disease.

• Structural Engineering and Mechanics
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• v.50 no.1
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• pp.89-103
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• 2014

According to experimental studies made so far, design formula of shear characteristics suggested by ISO 22762 and JEAG 4614, representative design code for Lead Rubber Bearing(LRB) shows dependence caused by changes in compressive stress. Especially, in the case of atypical special structure, such as a nuclear power structure, placement of seismic isolation bearing is more limited compared to that of existing structures and design compressive stress is various in sizes. As a result, there is a difference between design factor and real behavior with regards to shear characteristics of base isolation device, depending on compressive stress. In this study, a full-scale low hardness device of LRB, representative base isolation device was manufactured, analyzed, and then evaluated through an experiment on shear characteristics related to various compressive stresses. With design compressive stress of the full-scale LRB (13MPa) being a basis, changes in shear characteristics were analyzed for compressive stress of 5 MPa, 10 MPa, 13 MPa, 15 MPa, and 20 MPa based on characteristics test specified by ISO 22762:2010 and based on the test result, a regression analysis was made to offer an empirical formula. With application of proposed design formula which reflected the existing design formula and empirical formula, trend of horizontal characteristics was analyzed.

• Journal of Digital Convergence
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• v.18 no.8
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• pp.343-351
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• 2020

The purpose of this study was to investigate social networks, loneliness, and sleep quality related to health-related quality of life in older adults living alone. Data were collected from 111 community-dwelling elderly. The data were analyzed using descriptive statistics, independent t-test, one-way ANOVA, Pearson's correlation coefficients, and multiple linear regressions with IBM SPSS 26.0 program. In multiple regression analysis, physical component summary (PCS) was predicted by the level of education (β=.20, p=.020), social networks (β=.31, p=.012), and sleep quality (β=-.23, p=.011). The model including these variables accounted for 25.7% of the variance in the PCS. Mental component summary (MCS) was predicted by loneliness (β=-.37, p=.004). Loneliness accounted for 31.7% of the variance in the MCS. In order to enhance the health-related quality of life of the older adults living alone, the intervention program to resolve social isolation should be provided for them.

• Journal of the Korea Convergence Society
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• v.9 no.4
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• pp.285-301
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• 2018

The purpose of this study was to investigate the relationship between health-related physical fitness factors and cognitive function and isolation on 308 elderly over 65 years old in Seoul and Gyeonggi do. The measuring instrument were as follows demographic characteristics(age, education level, drink, spouse, participation on in exercise) and health-related physical fitness(grip strength, leg strength, flexibility, balance), cognitive function (MMSE-K, digit span test) and emotional/social aspects of loneliness and isolation was measured. As a results, first, depending on the degree of demographic characteristics of elderly had significant difference. Second, health-related physical fitness and cognitive function are positive correlated, negative correlation between cognitive function and isolation. Third, health-related physical fitness of the elderly had a significant effect on cognitive function. Fourth, health-related fitness of the elderly had a significant effect on isolation. Fifth, the cognitive function of the elderly had a significant effect on isolation. In conclusion, this study will provide basic data for future aged man welfare studies and contribute to extended studies.

• Journal of the Earthquake Engineering Society of Korea
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• v.12 no.1
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• pp.67-77
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• 2008

Seismic isolation has been studied continuously as a solution of the seismic engineering to reduce the sectional forces and the damages of structures caused by earthquakes. To certify reliable design and installation of the seismic isolation systems, seismic isolation bearings should be fabricated under well planned quality control process, and proper evaluation tests for their seismic performance should be followed. In this study, shear property evaluation tests for the lead rubber bearings(LRB) and the rubber bearings(RB) were implemented and the temperature dependency tests were also implemented to evaluate the changes of shear properties according to the changes of temperature. After evaluation tests, the measured shear properties were compared to their design values and their deviation was analyzed comparing with the allowable error ranges specified in Highway Bridge Design Specifications. These results showed that a considerable number of isolation bearings have so large deviations from their design values that their error ranges were over or very close to the allowable ranges. And the test results for temperature dependency showed that the shear properties of isolation bearings would be changed in great degree by the change of temperature during their service period. If these two types of changes in their shear properties are superposed, it would possible that the changes of shear properties from their original design values are over than 50%.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.24 no.2 s.173
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• pp.370-377
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• 2000

This paper presents a mixed-mode type ER(electro-rheological) engine mount, and its vibration control performance for a passenger vehicle is presented. The field-dependent yield stress of a transfo rmer oil-based ER fluid is empirically distilled in both shear and flow modes. This is then incorporated with the governing equation of motion of the proposed mixed-mode(shear mode plus flow mode) type engine mount. The damping force is analyzed with respect to the intensity of the electric field and design parameters such as electrode gap. Subsequently, the ER engine mount which is equivalent to the conventional hydraulic engine mount in terms of the damping level is designed and manufactured. Both computer simulation and experimental test are undertaken in order to evaluate vibration isolation performance. In addition, this performance is compared with that of the conventional hydraulic engine mount.

• The Journal of the Korean Society for Microbiology
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• v.34 no.5
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• pp.471-478
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• 1999

Spirochetes were isolated from the midgut of Ixodes persulcatus ticks captured at Chungju, Korea and identified as Borrelia afzelii strains by polymerase chain reaction. To determine the pathogenicity of the B. afzelii strains isolated in Korea, the microbiological and pathological features of Lyme disease were observed in C3H/He mice after intraperitoneal inoculation of the fresh isolate of B. afzelii strain. The results are summarized as follows 1) The Borrelia were detected in the tissues of heart, spleen, kidney, urinary bladder and knee joint within 7 days after inoculation of infection by dark field microscopic examination. The isolation rate from heart, urinary bladder and joint was significantly higher than the rate from spleen, kidney, and blood samples. 2) The Borrelia was detected in heart muscle by indirect immunofluorescent antibody test. 3) Antibody to the Borrelia was detected as early as one week after inoculation. 4) The marked tropism of the Borrelia was observed in myocardial, urinary tract and joint tissue. The main pathological features are inflammation in tissues of heart, kidney, joint and urinary bladder. From these results, the Borrelia afzelii strain isolated in Korea were determined as pathogenic strain.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2005.05a
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• pp.383-386
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• 2005

Low frequency heavy-weight impact noise is the most irritating noise in Korean high-rise reinforced concrete apartment buildings. This low frequency noise is generated by foot traffic due to the fact that Koreans do not wear shoes at home. The transmission of the noise is facilitated by a load bearing wall structural system without beams and columns which is used in these buildings. In order to control low frequency heavy-weight impact noise, floating floors using isolation materials such as glass-wool mat and poly-urethane mat are used. However, it was difficult to control low frequency heavy-weight impact sound using isolation material. In this study, reinforcement of concrete slab using beams and plate was conducted. Using the FEM analysis, the effect of concrete slab reinforcement using FRP(fiber-glass reinforced plastic) on the bang machine impact vibration acceleration level and sound were conducted at the standard floor impact sound test building. The $3{\sim}4dB$ floor impact vibration acceleration level and impact sound pressure level were reduced and the natural frequency of slabs were changed.