• Title/Summary/Keyword: Isolation Test

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First Report on Isolation of Salmonella Enteritidis from Eggs at Grocery Stores in Korea

  • Kim, Young Jo;Song, Bo Ra;Lim, Jong Su;Heo, Eun Jeong;Park, Hyun Jung;Wee, Sung Hwan;Oh, Soon Min;Moon, Jin San
    • Food Science of Animal Resources
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    • v.33 no.2
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    • pp.239-243
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    • 2013
  • Salmonella Enteritidis is responsible for causing foodborne diseases upon consumption of egg products. While cases of S. Enteritidis isolation from eggs have been reported in other countries, no such cases have previously been reported in Korea. In this study, we report the first isolation and identification of S. Enteritidis from domestically distributed eggs in Korea. Eggs were collected from eight countrywide grocery stores during different seasons between 2011 and 2012. Egg contents and washing solution of egg shells were incubated in buffered peptone water, and the enriched broth was further enriched in tetrathionate broth and Rappaport-Vassiliadis. The secondary enriched broth was streaked on xylose lysine desoxycholate agar. The suspected colonies were confirmed to S. Enteritidis by a biochemical test, serotyping, and PCR test. Genetic relatedness among the isolates was analyzed using Diversilab Salmonella kit. Three strains of S. Enteritidis were isolated from egg contents and egg shells collected from grocery stores of the Eumseong-city in the fall of 2011. All three stains showed resistance to chloramphenicol, streptomycin, nalidixic acid, and ampicillin by the disk diffusion method. In addition, the isolates showed more than 99% DNA homology, indicating that they were presumably identical strains. Therefore, there is a requirement to monitor and control against S. Enteritidis from eggs in Korea.

Isolation and Identification of Aseptic Meningitis Virus in Pusan, 1998 (부산지역 무균성 뇌막염 원인 바이러스의 분리 및 동정 - 1998년을 중심으로)

  • 조경순;김만수;정구영;민상기;구평태;김병준;윤재득;지영미;김기순
    • Journal of Environmental Science International
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    • v.8 no.2
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    • pp.165-169
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    • 1999
  • The incidence of aseptic meningitis infection is ensuing and threatening the health of children. Enteroviruses are the major agents of aseptic meningitis and identification of virus has been a clue to diagnosis and epidemiology. The outbreak of aseptic meningitis occurred in Pusan, 1998. Patients were concentrated from April through November. Children were more susceptible than adults. Among 306 cases of specimens from stool, throat swab tested, only 7.2% were positive on virus isolation, 12 cases from stool and 10 from throat, respectively. All isolated 7 serotypes of viruses represented cytopathic effect on cultured cells. Three types of echovirus 6.25, 30 and coxsackievirus B2, B3, B4, B6 were identified by neutralizing antibody test. Isolated coxsackievirus and echovirus were observed by an electron microscope with negative staining.

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Isolation and Screening of Pepsin Inhibitor-Producing Actinomycetes (Pepsin 저해물질을 생산하는 방선균의 분리 및 검색)

  • 박석규;성낙계;노종수
    • Microbiology and Biotechnology Letters
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    • v.17 no.2
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    • pp.115-120
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    • 1989
  • For the purpose of obtaining microorganisms which produced an extracellular pepsin inhibitor, screening test was carried out. One strain of Actinomycetes (GF 155-2) isolated from soil samples showed a high inhibitory activity against porcine pepsin. The morphological, physiological and cultural characteristics of the strain GE 155-2 on various culture media were studied according to ISP methods and Bergey's manual of determinative bacteriology (8th ed.). This Actinomycetes GE 155-2 was found to be similar to the genus Microtetraspora.

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Development of wind tunnel test model of mid-rise base-isolated building

  • Ohkuma, Takeshi;Yasui, Hachinori;Marukawa, Hisao
    • Wind and Structures
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    • v.7 no.3
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    • pp.203-214
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    • 2004
  • This paper describes a method for developing a multi-degree-of freedom aero-elasto-plastic model of a base-isolated mid-rise building. The horizontal stiffness of isolators is modeled by several tension springs and the vertical support is performed by air pressure from a compressor. A lead damper and a steel damper are modeled by a U-shaped lead line and an aluminum line. With this model, the frequency ratio of torsional vibration to sway vibration, and plastic displacements of isolation materials can be changed easily when needed. The results of isolation material tests and free vibration tests show that this model provides the object performance. The peak displacement factors are about 4.5 regardless of wind speed in wind tunnel tests, but their gust response factor decreases with increment of wind speed.

FDI considering Two Faults of Inertial Sensors (관성센서의 이중 고장을 고려한 고장 검출 및 분리)

  • 김광훈;박찬국;이장규
    • Journal of Institute of Control, Robotics and Systems
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    • v.10 no.1
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    • pp.1-9
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    • 2004
  • Inertial navigation system with hardware redundancy must use FDI(Fault Detection and Isolation) method to remove the influence of faulty sensors. Until now, several FDI methods such as PSA(Parity Space Approach), GLT(Generalized Likelihood ratio Test) and OPT(Optimal Parity vector Test) method are generally used. However, because these FDI methods only consider the situation that the system has one faulty sensor, these methods cannot be directly adapted for the system with two faulty sensors. To solve this problem, in this paper, PSA method is analyzed and based on this result, new FDI method called EPSA is proposed to consider a detection and an isolation of two faulty sensors in inertial navigation system.

Estimation of flanking transmission due to difference between laboratory and field test (실험실 및 현장실험을 통한 벽체의 우회전달음 평가에 관한 연구)

  • Chung, J.Y.;Lee, S.H.
    • Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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    • 2006.05a
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    • pp.1383-1386
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    • 2006
  • This study examines the difference of airborne sound isolation between laboratory and field test. The airborne sound isolation between adjacent dwellings in multi-family buildings is often much less than would be expected from the rated sound reduction index of the nominally-separating wall, due to structure-borne transmission of vibration at the junctions of wall. A variety of construction modifications to control such transmission have also been evaluated. This study presents a factor of the difference for flanking involving joint of wall, and shows the effect of some practical modifications that control the key flanking paths

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Dynamic Property Evaluation of Friction Pendulum Isolation Bearing (마찰진자 베어링의 동적 특성평가)

  • 이경진;김갑순;서용표
    • Proceedings of the Earthquake Engineering Society of Korea Conference
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    • 2003.03a
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    • pp.465-472
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    • 2003
  • The main concept of base isolation system is to reduce the member forces by decreasing the earthquake forces transmitted to superstructure instead of the conventional techniques of strengthening the structural members. There are two important advantages in friction pendulum systems. The functions of carrying the vertical load and of providing horizontal stiffness are effectively separated. This results in a more stable system that eliminates the need of a fail-safe mechanism. Friction pendulum systems are less sensitive to variations in the frequency content of ground excitation and tend to limit the intensity of the farce imparted to the superstructure. This study investigates the friction coefficients on the FPS test specimens according to the velocity, bearing pressure and test waveform.

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A Teleoperated Cleaning Robot for a High Radioactive Environment

  • Kim, Ki-Ho;Park, Jang-Jin;Yang, Myung-Seung;Oh, Chae-Youn
    • 제어로봇시스템학회:학술대회논문집
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    • 2003.10a
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    • pp.849-854
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    • 2003
  • The Korea Atomic Energy Research Institute has developed a teleoperated cleaning robot for use in the radioactive zone of the isolation room of the Irradiated Material Examination Facility where direct human access to the interior is strictly limited. The teleoperated cleaning robot that was designed to completely eliminate human interaction with the hazardous radioactive contaminants has five remotely replaceable submodules - a mobile module for navigation, a cleaning module for dislodging and sucking contaminated waste, a sensing module for obstacle avoidance, a collection module for storing the acquired waste, and a cover module for protecting the collection module. This cleaning robot is capable of cleaning the contaminated floor surface of the isolation room and collecting loose dry spent nuclear fuel debris and other radioactive waste fixed or scattered on the floor surface. The developed cleaning robot is operated either by a manual control or by autonomous control in conjunction with a graphical simulator, by which the human operator can monitor and intervene the robot performing cleanup tasks in the isolation room. In this paper, we present the mechanical and environmental design considerations and development of the teleoperated cleaning robot for radioactive isolation room use. We also demonstrate its mock-up performance test results from the viewpoint of a remote cleanup operation and remote maintenance.

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On-orbit Micro-vibration Isolation Performance Verification for Spaceborne Cryocooler Passive Vibration Isolator Using SMA Mesh Washer (SMA 메쉬 와셔를 적용한 우주용 냉각기 수동형 진동절연기의 궤도 미소진동 절연성능 검증)

  • Kwon, Seong-Cheol;Jeon, Su-Hyeon;Oh, Hyun-Ung
    • Transactions of the Korean Society for Noise and Vibration Engineering
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    • v.25 no.1
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    • pp.24-32
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    • 2015
  • Pulse tube-type spaceborne cryocooler is widely used to cool down the infrared sensor of observation satellites. However, such cryocooler also generates micro-vibration which is the one of main sources to seriously affect the image quality during its on-orbit operation. Therefore, to comply with the mission requirement of high resolution observation satellite, additional technical efforts have been required. In this study, we proposed a spaceborne cryocooler passive vibration isolator using SMA mesh washer, which guarantees the structural safety of both the micro-vibration disturbance source and itself under harsh launch vibration loads without an additional holding mechanism and the micro-vibration isolation performance on orbit environment. To verify the micro-vibration isolation performance of the proposed vibration isolator, we performed the micro-vibration isolation measurement test using the dedicated micro-vibration measurement device proposed in this study.

A Study on the Transmitted Energy Contribution Analysis of SUV Engine Mount by Vibration Power Flow Measurement (진동 파워흐름 측정을 통한 SUV용 엔진 마운트의 에너지 전달 기여도 분석에 관한 연구)

  • Kim, Su-Gon;Lee, Sang-Kwon;Kim, Sung-Jong
    • Transactions of the Korean Society for Noise and Vibration Engineering
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    • v.18 no.4
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    • pp.400-410
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    • 2008
  • Reduction of structure-borne noise in the compartment of a car is an important task in automotive engineering. Many methods which analyze noise transfer path have been generally used for structure-borne noise. These methods are useful in solving particular problem but do not quantify the effectiveness of vibration isolation for each isolator of a vehicle. To quantify the effectiveness of vibration isolation, the vibrational power flow measurement has been used for a simple isolation system or a laboratory based isolation system. This paper identifies the transfer path of booming noise in a SUV. The powertrain used for test has a in-line 4cylinder engine and 5-shift auto-transmission. This powertrain is transversely supported by four isolators. We calculated the energy flow throughout four isolator by the measurement of power flow and the contribution of energy flow at each isolator.