• Transactions of the Korean Society of Pressure Vessels and Piping
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• v.14 no.2
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• pp.88-95
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• 2018

In this study, to enhance the seismic performance of nuclear power plants (NPP), a small laminated rubber bearing (LRB) is chosen as a seismic design option of the vulnerable equipment. Prior to the application of equipment base isolation, it is necessary to review the feasibility that the technique contributes enough to the seismic performance of NPP by analysis. At first, some preliminary design of small LRBs for equipment is carried out. Design parameters such as horizontal and vertical stiffnesses, design natural frequencies are checked by calculation and analysis for the four design options considering various upper weights. Performance test of small LRB is to be carried out to verify static performance using the results.

• Journal of Marine Bioscience and Biotechnology
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• v.12 no.1
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• pp.50-58
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• 2020

Sphacelaria is a filamentous brown algal genus that can be epibiotic on macroalgae, marine plants, and sea turtles. Its important role in benthic ecosystems, exposure to different stressors (e.g., grazing), and use as a model organism make Sphacelaria ideal for assessing physiological responses of organisms to environmental inputs. Single-cell RNA sequencing is a powerful new probe for understanding environmental responses of organisms at the molecular (transcriptome) level, capable of delineating gene regulation in different cell types. In the case of plants, this technique requires protoplasts ("naked" plant cells). The existing protoplast isolation protocols for Sphacelaria use non-commercial enzymes and are low-yielding. This study is the first to report the production of protoplasts from Sphacelaria fusca (Hudson) S.F. Gray, using a combination of commercial enzymes, chelation, and osmolarity treatment. A simple combination of commercial enzymes (cellulase Onozuka RS, alginate lyase, and driselase) with chelation pretreatment and an increased osmolarity (2512 mOsm/L H₂O) gave a protoplast yield of 15.08 ± 5.31 × 10⁴ protoplasts/g fresh weight, with all the Sphacelaria cell types represented. Driselase had no crucial effect on the protoplast isolation. However, the increased osmolarity had a highly significant and positive effect on the protoplast isolation, and chelation pretreatment was essential for optimal protoplast yield. The protocol represents a significant step forward for studies on Sphacelaria by efficiently generating protoplasts suitable for cellular studies, including single-cell RNA sequencing and expression profiling.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.13 no.1
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• pp.19-25
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• 2002

This paper presents novel architectures for power amplifier (PA) relying on 3rd harmonic-tuning technique and dual feeding antenna structure for the isolation of the Tx and the Rx ports. Active integrated antenna (AIA) with power amplifier makes the problem of the isolation between the Tx and the Rx ports occur So, this paper suggests dual feeding and dual resonant structures of the AIA with PA are possible to obtain the high isolation between the Tx and the Rx signals. Dual resonant triangular microstrip antenna, which can replace power amplifier tuning circuit, with slots-loaded and characteristic of the isolation between the Tx and the Rx ports using inset microstrip line feeding and probe feeding methods is proposed and experimentally studied for the case of thin substrate.

• Earthquakes and Structures
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• v.9 no.3
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• pp.603-623
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• 2015

The scale and nature of the recent earthquakes in the world and the related earthquake disaster index coerce the concerned community to become anxious about it. Therefore, it is crucial that seismic lateral load effect will be appropriately considered in structural design. Application of seismic isolation system stands as a consistent alternative against this hazard. The objective of the study is to evaluate the structural and economic feasibility of reinforced concrete (RC) buildings with base isolation located in medium risk seismic region. Linear and nonlinear dynamic analyses as well as linear static analysis under site-specific bi-directional seismic excitation have been carried out for both fixed based (FB) and base isolated (BI) buildings in the present study. The superstructure and base of buildings are modeled in a 3D finite element model by consistent mass approach having six degrees of freedom at each node. The floor slabs are simulated as rigid diaphragms. Lead rubber bearing (LRB) and High damping rubber bearing (HDRB) are used as isolation device. Change of structural behaviors and savings in construction costing are evaluated. The study shows that for low to medium rise buildings, isolators can reduce muscular amount of base shears, base moments and floor accelerations for building at soft to medium stiff soil. Allowable higher horizontal displacement induces structural flexibility. Though incorporating isolator increases the outlay, overall structural cost may be reduced. The application of base isolation system confirms a potential to be used as a viable solution in economic building design.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2002.10a
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• pp.849-853
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• 2002

A specific experimental method, the split Hopkinson pressure bar (SHPB) technique has been widely used to determine the dynamic material properties under the impact compressive loading conditions with strain-rate of the order of 103/s~104/s. In this paper, dynamic deformation behaviors of rubber materials widely used for the isolation of vibration from varying structures under dynamic loading are determined using a Split Hopkinson Pressure Bar technique.

• Journal of Korean Foot and Ankle Society
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• v.15 no.3
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• pp.144-148
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• 2011

Purpose: To investigate the results of percutaneous repair technique of Achilles tendon ruptures, and to describe the surgical technique. Materials and Methods: We retrospectively analyzed the outcomes of 73 patients with ruptured Achilles tendon from October 1995 to September 2009. 28 patients were excluded due to short follow up period. 34 patients were male and 11 patients were female. The mean patient age was 37.19 (10~62) years. The location of rupture site was 6.58 cm proximal to the tendon insertion into the calcaneus on average. Mean follow up period was 55 months and All patients were surgically repaired using percutaneous technique with sural nerve isolation. Results: Arner-lindholm score were excellent in 32 (71%), good in 12 (27%), poor in 1 (2%) case. 44 cases (98%) had the score more than good. Mean American Orthopedic Foot and Ankle Society (AOFAS) Ankle-Hindfoot function score was 92.93 (67~100). We had 1 case of superficial infection, 1 case of soft tissue irritation by suture knot. Conclusion: Percutaneous repair with sural nerve isolation in treating ruptured Achilles tendon showed low complication rate and reliable clinical outcome.

• Journal of Embryo Transfer
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• v.31 no.1
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• pp.73-80
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• 2016

An in vitro assay following culture of endometrial epithelial cells is essential for understanding epithelial cell function in reproduction. Several diverse techniques have been developed for isolating endometrial epithelial cells, although an optimal technique has not been identified. In this study, we describe a sedimentation-adherence (S-A) isolation technique with a high-yield cell-separating ability to isolate endometrial epithelial cells from 8-week-old female C57BL/6 mice. We analyzed total cell number, viability, morphology, and expression of cytokeratin 18 as an endometrial epithelial cell-specific marker in cells isolated using a mechanical method compared to the S-A technique. There were no significant differences in the total number, viability, or morphology of the putative endometrial epithelial cells with either method. In contrast, significantly more endometrial epithelial cells harvested using the S-A method were positively stained for cytokeratin 18 than those isolated using the mechanical method. These results confirm that the S-A method is more efficient for retrieving endometrial epithelial cells than a mechanical method.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.21 no.11
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• pp.1235-1239
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• 2010

In this work, a wideband technique has been proposed that improves the IF bandwidth of mixers and a 24-GHz down-conversion mixer employing the proposed technique has been designed and fabricated based on 0.13-${\mu}m$ RFCMOS technology. The mixer showed the conversion gain of $2.7{\pm}1.5$ dB from DC to 5.25 GHz IF for a fixed LO frequency of 24 GHz. Measured P-1dB and LO-RF isolation was -8.7 dBm and 21 dB, respectively. The mixer draws DC current of 10.6 mA from 1.3 V supply.

• Earthquakes and Structures
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• v.15 no.5
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• pp.513-528
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• 2018

A typical viable technique to decrease the seismic response of liquid storage tanks is to isolate them at the base. Base-isolation systems are an efficient and feasible solution to reduce the vulnerability of structures in high seismic risk zones. Nevertheless, when liquid storage tanks are under long-period shaking, the base-isolation systems could have different impacts. These kinds of earthquakes can damage the tanks readily. Hence, the seismic behaviour and vibration of cylindrical liquid storage tanks, subjected to earthquakes, is of paramount importance, and it is investigated in this paper. The Finite Element Method is used to evaluate seismic response in addition to the reduction of excessive liquid sloshing in the tank when subjected to the long-period ground motion. The non-linear stress-strain behaviour pertaining to polymers and rubbers is implemented while non-linear contact elements are employed to describe the 3-D surface-to-surface contact. Therefore, Nonlinear Procedures are used to investigate the fluid-structure interactions (FSI) between liquid and the tank wall while there is incompressible liquid. Part I, examines the effect of the flexibility of the isolation system and the tank aspect ratio (height to radius) on the tank wall radial displacements of the tank wall and the liquid sloshing heights. Maximum stress and base shear force for various aspect ratios and different base-isolators, which are subjected to three seismic conditions, will be discussed in Part II. It is shown that the composite-base isolator is much more effective than other isolators due to its high flexibility and strength combined. Moreover, the base isolators may decrease the maximum level pertaining to radial displacement.

• Proceedings of the KOSOMBE Conference
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• v.1996 no.11
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• pp.277-280
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• 1996

We have developed a ground-isolation circuit in order to reduce the noise of the internal controller system for the total artificial heart(TAH) and ventricular assist device(VAD). Using the ground-isolation technique, we could transmit the analog target signal to other pheriperal device including IBM PC via RS232C and polygraph, with no noise. Experimental results of VAD showed that there was less impulsive noise in current signal which caused in our previous conventional system. Therefore it could be proved that implementation of isolation technique is very effective to improve the signal to noise ratios of analog signal transmission for TAH or VAD.