• Title/Summary/Keyword: Isolation Forest

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Isolation and Characterization of Extracellular Polymeric Substances (EPS)-producing bacteria for restoration of burnt forest soils (산불토양복원을 위한 Extracellular Polymeric Substances (EPS) 생성세균의 분리, 동정 및 특성에 관한 연구)

  • Lee, Gun-Young;Song, In-Geun;Chung, Jae-Chun;Kim, Young-Jun
    • Journal of the Korea Organic Resources Recycling Association
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    • v.12 no.4
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    • pp.139-147
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    • 2004
  • We have isolated two bacterial strains, FM-02 and AL-02, which produced EPS from forest soil for the restoration of forest fire by promoting soil aggregation. FM-02 was found to be Gram negative rod and belong to Beta Proteobacterium sp. through 16s-rDNA sequence analysis, and AL-02 was Gram positive rod and showed 81% of similarity to Zoogloea sp. through the analysis of 16s-rDNA sequence. FM-02 and AL-02 produced about 1.8g and 8.3g of EPS, respectively, per 1L of culture as dry weight. Flocculation activity (FA) was also measured in two strains. FM-02 showed 2.31 FA against active carbon, and AL-02 showed 6.21 FA against kaolin clay. From these results, we expect that AL-02 strain will be applied as a good biological material for the reduction of forest soil erosion by wild and rain after fire through promoting coagulation of soil particles.

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Isolation and Identification of Stilbene glycosides from the Bark of Pinus koraiensis (잣나무 수피의 Stilbene glycosides의 분리 및 동정)

  • Song, Hong-Keun
    • Journal of the Korean Wood Science and Technology
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    • v.29 no.4
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    • pp.97-102
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    • 2001
  • EtOAc extract from the bark of Pinus koraiensis Sieb. et Zucc was isolated by column chromatography which was packed with Sephadex LH-20 or TSK-gel HW-40F. Several stilbene glycosides were identified by $^1H{\cdot}^{13}C$-NMR, HMQC, HMBC and $FAB^+$ MS. Three stilbene glycosides, Z-pinostilbenoside, E-desoxyrhaponticin, and E-resveratroloside, were identified.

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Isolation and Characterization of Cinnamoyl CoA Reductase Gene from Codonopsis lanceolata

  • Kim Ha-Na;In Jun-Gyo;Lee Eun-Kyung;Yoon Jae-Ho;Lee Mee-Sook;Bae Hyeun-Jong;Yang Deok-Chun
    • Plant Resources
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    • v.8 no.3
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    • pp.181-187
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    • 2005
  • A cinnamoyl CoA reductase (CCR) cDNA (ClCCR) was isolated from tabroot mRNAs of Codonopsis lanceolata by cDNA library construction, and its expression was investigated in relation to abiotic stresses. The ClCCR is 1008 bp in length with an open reading frame (ORF) of 336 amino acids. The deduced amino acid sequence was showed high similarity with cinnamoyl-CoA reductases of P. tremuloides (AAF43141) 87%, F.${\times}$aranassa (AAP46143) 83%, L. album (CAD29427) 80%, E. gunnii (CAA66063) 72%, S. tuberosum (AAN71761) 83%. Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis was revealed that the ClCCR expression was regulated by abiotic stresses.

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The Collected data-based Air Pollutant Emission Prediction for construction equipment in Construction Sites (건설장비의 배출가스 데이터 기반 대기오염물질 배출량 예측 시스템)

  • Noh, Jaeyun;Kim, Yujin;Kim, Sumin;Han, Seungwoo
    • Proceedings of the Korean Institute of Building Construction Conference
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    • 2021.11a
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    • pp.86-87
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    • 2021
  • As non-road mobile pollutants such as construction equipment are emerging as the main cause of air pollutants emission, construction equipment regulations are gradually strengthening. Research was conducted by correcting the emission coefficient to calculate and predict air pollutant emissions of construction equipment, but it did not reflect site variables such as field and equipment conditions that affect actual emissions. This study derived an Artificial Neural Network emission prediction model based on the actual emission data of excavators and trucks measured at the site and proposed a platform to predict the emission of air pollutants at the site according to the working size and conditions. Through this, it is possible to establish an eco-friendly process plan using a model from the construction plan.

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이상 탐지 기법을 활용한 IoT 센서 고장 진단에 관한 연구

  • 성상하;최형림;박도명;김상진
    • Proceedings of the Korean Institute of Navigation and Port Research Conference
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    • 2023.11a
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    • pp.185-187
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    • 2023
  • 고장 진단은 IoT 장비의 안전성과 효율성을 유지하는데 필요한 기술 중 하나이다. 따라서, 본 연구는 IoT 센서 데이터를 기반한 고장 진단 알고리즘을 개발하는데 목적이 있다. 본 연구는 알고리즘의 효율성을 개선하기 위해 기술통계량을 기반하여 데이터 차원을 축소하였으며, 이를 바탕으로 고장 진단 알고리즘의 정확도 및 연산시간을 개선하였다. 본 연구는 다양한 후보 알고리즘을 활용하여 고장진단을 수행하였으며, 정확도를 기반으로 가장 우수한 알고리즘을 선정하였다. 연구 결과, Isolation Forest 알고리즘이 가장 뛰어난 분류 결과를 나타내었다. 본 연구결과를 통해 IoT 센서의 안전성과 신뢰성을 향상시키는 데 도움을 줄 수 있다.

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Design and evaluation of artificial intelligence models for abnormal data detection and prediction

  • Hae-Jong Joo;Ho-Bin Song
    • Journal of Platform Technology
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    • v.11 no.6
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    • pp.3-12
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    • 2023
  • In today's system operation, it is difficult to detect failures and take immediate action in the case of a shortage of manpower compared to the number of equipment or failures in vulnerable time zones, which can lead to delays in failure recovery. In addition, various algorithms exist to detect abnormal symptom data, and it is important to select an appropriate algorithm for each problem. In this paper, an ensemble-based isolation forest model was used to efficiently detect multivariate point anomalies that deviated from the mean distribution in the data set generated to predict system failure and minimize service interruption. And since significant changes in memory space usage are observed together with changes in CPU usage, the problem is solved by using LSTM-Auto Encoder for a collective anomaly in which another feature exhibits an abnormal pattern according to a change in one by comparing two or more features. did In addition, evaluation indicators are set for the performance evaluation of the model presented in this study, and then AI model evaluation is performed.

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Studies on Xylan of Tropical Hardwood (II) -Isolation and Purification of Xylan- (열대산(熱帶産) 광엽수재(廣葉樹材)의 Xylan에 관(關)한 연구(硏究) (II) -Xylan의 단리(單離) 및 정제(精製)-)

  • Lee, Jong Yoon
    • Journal of Korean Society of Forest Science
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    • v.35 no.1
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    • pp.24-32
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    • 1977
  • According to the chlorous salt method, most of holocellulose whose lignin was removed, was obtained. In extracting xylan from holocellulose by the different densities of alkali, 5% KOH was extracted three times but still there remained part of xylan in it and another composite of hemicellulose and cellulose was obtained. The extraction of 10% and 20% KOH showed a desirable result. Rather than the ordinary method to use a large quantity of ethanol in the precipitation isolation of xylan, the method to use a small quantity of ethanol in adopting the dialysis with cellophane-membrane by condensing density to one tenth, made il possible to extract a high purity xylan in a high retrieving rate. In isolating glucomannan, the residue of 5% KOH extraction contained a large quantity of xylan, the residue of 10% and 24% KOH extraction, also showed the same result and the comparison between glucose and mannose was approximately 1 : 1. The purification of Fehling solution made it possible to obtain comparatively pure xylan but the process of oxidation dissolution was complicated and the retrieving rate was low. This was not a good method. The ethanol titration purified a high purity xylan in a high retrieving rate and was a very excellent purifying method, considering its simple and easy process. These two purifying methods, however, could not completely remove the residue of arabinose. This will be examined and reported later.

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Isolation and Identification of Plant-Growth-Promoting Bacteria and Their Effect on Growth of Red Pepper(Capsicum annuum L.) (식물생육촉진(植物生育促進) 세균(細菌) 분리(分離), 동정(同定)과 고추에 대한 처리효과(處理效果))

  • Lee, Young-Han;Yun, Han-Dae;Ha, Ho-Sung
    • Korean Journal of Soil Science and Fertilizer
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    • v.29 no.1
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    • pp.67-73
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    • 1996
  • This study was conducted to determine the effect of treatment with the plant-growth-promoting bacteria on the growth of red pepper(Capsicum annuum L.).The eight plant-growth-promoting bacteria were isolated from the humic soil in the forest region. The isolated bacteria(IB) was identified by the method of the biochemical test(API kit) and the composition of the fatty acid(MIDI system).The IBs were inoculated by spray of 17ml at 72 cell tray filled with peatmoss every week. respectively, with mixed liquid eulture of eight strains. The IBs were identified as Micrococcus sp.. Bacillus subtilis. Enterobacter agglomerans, Bacillus megaterium, Pseudomonas putida. Pseudomonas fluorescens, Xanthomonas maltophilia and Staphylococcus xylosus by API kit and MIDI system. The plant height number of leaves and leaf length of red pepper grown on peatmoss treated with the IB were better than those of nontreatment at the 10th day after inoculation.

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Isolation and characterization of cellulolytic bacteria, Bacillus sp. EFL1, EFL2, and EFP3 from the mixed forest (혼효림으로부터 셀룰로오스분해 박테리아 분리 및 효소학적 특성규명)

  • Park, Hwa Rang;Oh, Ki-Cheol;Kim, Bong-Gyu
    • Journal of Applied Biological Chemistry
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    • v.61 no.1
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    • pp.59-67
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    • 2018
  • This study was conducted to isolate the cellulolytic bacteria able to grow on LB- Carboxymethyl cellulose (CMC) agar trypan blue medium from the mixed forest and Larix leptolepis stands. Three bacterial strains with high activity against both CMC and xylan were isolated. Both API kit test and 16S rRNA gene sequence analysis revealed that the three different isolates belong to the gene Bacillus. Therefore, the isolates named as Bacillus sp. EFL1, Bacillus sp. EFL2, and Bacillus sp. EFP3. The optimum growth temperature of Bacillus sp. EFL1, EFL2, and EFP3 were $37^{\circ}C$. The optimum temperature for CMCase and xylanase from Bacillus sp. EFL1 were $50^{\circ}C$. The optimum pH of Bacillus sp. EFL1 xylanase was pH 5.0 but the optimum pH of CMCase from Bacillus sp. EFL1 was pH 6.0. The optimum temperature of CMCase and xylanase from Bacillus sp. EFL2 was $60^{\circ}C$, respectively. The optimum pH of CMCase of Bacillus sp. EFL2 was 5.0, whereas xylanase showed high activity at pH 3.0-9.0. The optimum temperature for CMCase and xylanase of Bacillus sp. EFP3 was $50^{\circ}C$. The optimum pH for CMCase and xylanse was 5.0 and 4.0, respectively. CMCases from Bacillus sp. EFL1, EFL2, and EFP3 were thermally unstable. Although xylanase from Bacillus sp. EFL1 and EFP3 showed to be thermally unstable, xylanase from Bacillus sp. EFL2 showed to be thermally stable. Therefore, Bacillus sp. EFL2 has great potential for animal feed, biofuels, and food industry applications.