• Journal of Microbiology and Biotechnology
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• v.29 no.10
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• pp.1665-1674
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• 2019

Zika virus (ZIKV) is a mosquito-transmitted, emerging Flavivirus that causes Guillain-$Barr{\acute{e}}$ syndrome and microcephaly in adults and fetuses, respectively. Since ZIKV was first isolated in 1947, severe outbreaks have occurred at various places worldwide, including Yap Island in 2007, French Polynesia in 2013, and Brazil in 2015. Although incidences of ZIKV infection and dissemination have drastically increased, the mechanisms underlying the pathogenesis of ZIKV have not been sufficiently studied. In addition, despite extensive research, the exact roles of individual ZIKV genes in the viral evasion of the host innate immune responses remain elusive. Besides, it is still possible that more than one ZIKV-encoded protein may negatively affect type I interferon (IFN) induction. Hence, in this study, we aimed to determine the modulations of the IFN promoter activity, induced by the MDA5/RIG-I signaling pathway, by over-expressing individual ZIKV genes. Our results show that two nonstructural proteins, NS2A and NS4A, significantly down-regulated the promoter activity of IFN-${\beta}$ by inhibiting multiple signaling molecules involved in the activation of IFN-${\beta}$. Interestingly, while NS2A suppressed both full-length and constitutively active RIG-I, NS4A had inhibitory activity only on full-length RIG-I. In addition, while NS2A inhibited all forms of IRF3 (full-length, regulatory domain-deficient, and constitutively active), NS4A could not inhibit constitutively active IRF3-5D. Taken together, our results showed that NS2A and NS4A play major roles as antagonists of MDA5/RIG-I-mediated IFN-${\beta}$ induction and more importantly, these two viral proteins seem to inhibit induction of the type I IFN responses in differential mechanisms. We believe this study expands our understanding regarding the mechanisms via which ZIKV controls the innate immune responses in cells and may pave the way to development of ZIKV-specific therapeutics.

• Korean Journal of Environment and Ecology
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• v.31 no.2
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• pp.152-165
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• 2017

The greater long-tail hamster, Tscherskia triton, is widely distributed in Northern China, Korea and adjacent areas of Russia. Except for its distribution, biological characteristics related to life history, behavior, and ecological influences for this species are rarely studied in Korea. This study was conducted to obtain biological information on breeding, growth and development that are basic to species-specific studies. The study adopted laboratory management of a breeding programme for T. triton collected in Jeju Island from March, 2015 to December, 2016. According to the study results, the conception rate was 31.67% and the mice in the large cages had a higher rate of conception than those in the small cages (56.7 vs. 6.7%). The gestation period was $22{\pm}1.6days$ (ranges from 21 to27 days), and litter size ranged from 2 to 7, with a mean of $4.26{\pm}1.37$ in the species. The minimum age for weaning was between $19.2{\pm}1.4days$ (range of 18-21 days). There were no significant differences by sex between mean body weight and external body measurements at birth. However, a significant sexual difference was found from the period of weaning (21 days old) in head and body length, as well as tail length (HBL-weaning, $106.50{\pm}6.02$ vs. $113.34{\pm}4.72mm$, p<0.05; HBL-4 months, $163.93{\pm}5.42$ vs. $182.83{\pm}4.32mm$, p<0.05; TL-4 months, $107.23{\pm}3.25$ vs. $93.95{\pm}2.15mm$, p<0.05). Gompertz and Logistic growth curves were fitted to data for body weight and lengths of head and body, tail, ear, and hind foot. In two types of growth curves, males exhibited greater asymptotic values ($164.840{\pm}7.453$ vs. $182.830{\pm}4.319mm$, p<0.0001; $163.936{\pm}5.415$ vs. $182.840{\pm}4.333mm$, p<0.0001), faster maximum growth rates ($1.351{\pm}0.065$ vs. $1.435{\pm}0.085$, p<0.05; $2.870{\pm}0.253$ vs. $3.211{\pm}0.635$, p<0.05), and a later age of maximum growth than females in head and body length ($5.121{\pm}0.318$ vs. $5.520{\pm}0.333$, p<0.05; $6.884{\pm}0.336$ vs. $7.503{\pm}0.453$, p<0.05). However, females exhibited greater asymptotic values ($105.695{\pm}5.938$ vs. $94.150{\pm}2.507mm$, p<0.001; $111.609{\pm}14.881$ vs. $93.960{\pm}2.150mm$, p<0.05) and longer length of inflection ($60.306{\pm}1.992$ vs. $67.859{\pm}1.330mm$, p<0.0001; $55.714{\pm}7.458$ vs. $46.975{\pm}1.074mm$, p<0.05) than males in tail length. These growth rate constants, viz. the morphological characters and weights of the males and females, were similar to each other in two types of growth curves. These results will be used as necessary data to study species specificity of T. triton with biological foundations.

• Journal of The Korean Society of Grassland and Forage Science
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• v.23 no.2
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• pp.77-80
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• 2003

This study was conducted to determine the optimum organic fertilizer rate (0, 100, 200, 300, 400, 500, 600kg/10a) of worm casting on growth response and yield of Ieol´ radish in Jeiu island. The results obtained were summarized as follows; plant height was getting bigger as organic fertilizer increased from 0 to 500kg/10a and then became smaller at 600kg/10a. But was no significance from 200 to 600kg/10a. Number of leaves, leaf width and root diameter were the same trend with plant height response. Root length was not significantly affected by organic fertilizers. SPAD reading value of leaves increased as increasing of organic fertilizers. Fresh matter yield (top+root) increased significantly 2,949∼4,561kg/10a as fertilizer rate increased from 0 to 200kg/10a, increased 5.096∼5,707kg/10a from 300 to 500kg/10a of fertilizer rate and decreased to 4,873kg/10a at 600kg/10a. Top and root weight were the same trend with fresh matter yield.

• Journal of the Korean Geographical Society
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• v.38 no.3
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• pp.389-399
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• 2003

CRN (Cosmogenic radionuclide) methodology has been a versatile tool applicable to a wide range of geomorphology. This study was underiaken to ascertain the rate of erosion and exposure age of mountain-top detritus (tors and block streams) on Mt. Maneo by employing the concentrations of in-situ produced cosmogenic $^{10}$ Be and $^{26}$ Al from bedrock surfaces that are exposed to cosmic rays. The results suggest that tors on the summit were positioned here during the glacial period but no later than 65ka and block streams have been stabilized also since the last glacial period but no later than 38ka. The tors on the summit have been eroded at a slower rate (9m/Ma) than blocks on the hillslope (15m/Ma) since the initial abrupt exposure of each landform to cosmic rays, suggesting that there is a slight difference in the rate of erosion between the summit and the hillslope, and that the local relief between the two areas has been increased. When the $^{26}$ Al/$^{10}$ Be-$^{10}$ Be concentrations from samples are plotted in Lal's steady-state erosion island, one sample (from a for) has complex exposure histories, which can be explained by the occurrence of multiple chipping event of 5cm to 60cm in length on the surface of the rock.

• Journal of Wetlands Research
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• v.15 no.2
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• pp.251-264
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• 2013

There are some factors affecting the shorebirds in selecting a coastal inland roost site where they have to stay during the high tide when the entire intertidal zone is covered with water. We investigated eight species (Eastern Curlews Numenius madagascariensis, Green Shanks Tringa nebularia, Bar-tailed Godwits Limosa lapponica, Grey Plovers Pluvialis squatarola, Dunlins Calidris alpine, Whimbrels Numenius phaeopus, Eurasian Curlews Numenius arquata and Terek Sandpipers Xenus cinereus) to identify the spatial and temporal variability in coastal inland roost use in the area and the factors influencing the use. We considered the area and length of the standing water in the roost site, temperature, wind speed, the time of migration and the intensity of disturbance. As a result, number of individuals and type of species using the roost site varied across spatial and temporal scales. And the factors affecting the roost use was species-specific. The roost site of the study area was a closed shrimp farm however, it has been converted to a Salicornia herbacea, a salt marsh plant, farm recently. In a situation where coastal inland roost site is needed, we hereby describe a resolution for the juxtaposition of shorebirds and farming.

• Journal of Bio-Environment Control
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• v.11 no.3
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• pp.144-148
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• 2002

The overall objective of this study was to determine which variety of carrot-10 Japan varieties or 6 domestic varieties- produces the optimum quality, when cultivated on Jeju Island. Plant height, leaf number and leaf weight were observed to be more superior in the purebred variety ‘Jungang’than other varieties. further studies, however, skewed the crossing variety ‘Bureoun’was superior to all others. Furthermore, when comparing root diameter, root length and root weight, the ‘Bibary’was superior in both the purebred and crossing variety, When researching Japan varieties, the crossing variety, ‘Jung’showed an increase in underground root. The purebred variety ‘Donghae’also showed an increase in underground root. Marketable yields in domestic varieties were greatest in ‘Hapa’, while in Japan varieties, ‘Kaneko’was the most productive. The percentage of root cracked was 15％ greater in ‘Bureon’. In the purebred variety, Jungang, the percentage was only 10％. The percentage of roots branched was highest in ‘Hidomi’which showed a rate of 9%, The roots branched rate was 2% for ‘Jung’and 6％ for‘Donghae’. Considering the percentage of roots clacked or branched, the marketability of carrots was higher in Japan varieties in comparison with domestic varieties. Therefore we conclude that, in the cropping patterns of carrots, the domestic variety has an advantage over the Japan variety.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.37 no.3
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• pp.174-180
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• 2001

Dusky spinefoot, Siganus fuscescens live primarily in shore reefs with sea weeds, like around Jeju Island in Korean south sea. Therefore they are caught much by set net in the coast of Jeju Island. We carried out measurements of the dorsal aspect target strengths of Dusky spinefoot in order to study the influences of swim bladder for fishes. and have obtained some desired results though not sufficiently complete as comparison with target strengths of fishes before and after the air gas in the swim bladder of fish is taken off by an injector. The appropriate equations share the common form TS = A + 20 log L. TS = B + 20/3 log W where TS is the average dorsal aspect target strength in decibels. L is the fish total length in centimeters. W is the fish total weight in grams, and the coefficient A and B are determined by a least mean squares regression analysis. The results obtained before the air gas in the swim bladder of fish is taken off are TS = -63.16 + 20 log L TS = -50.50 + 20/3 low W and, the results obtained after the air gas in the swim bladder of fish is taken off are TS = -65.29 + 20 log L TS = -52.62 + 20/3 log W As above result, the differences of target strengths before and after the air gas is taken off from swim bladder are 2.12-2.13 dB, but the maximum difference of target strengths of both cases averaged according to the fish aspects from $-45^\circ$ to $45^\circ$ is 5.87 dB, and the average difference of them is 2.01 dB.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.38 no.3
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• pp.241-248
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• 2002

The combined hydroacoustic and demersal trawl surveys to investigate the distribution characteristics of fish schools in the Yellow Sea and the East China Sea were carried out during the spring of 1997 by the training ship "Nagasaki Maru" of Nagasaki University. Fish samples were collected by bottom trawling from 9 trawl stations randomly selected in the survey area, and the species and length compositions of trawl catches are examined. Hydroacoustic data were collected by using a Furuno FQ-50 scientific echo sounder operating at 50KHz and the data stored in field were later processed in the laboratory. The results obtained can be summarized as follows ：1. In the 9 trawl surveys conducted in the Yellow Sea and the East China Sea, 78 species including 80 species of fishes, 4 species of Cephalopoda and 6 species of Crustacea, were identified. The most abundant species in these stations were swimming crab(Portunus trituberculatus), Japanese horse mackere(Trachurus japonicus), redlip croaker(Larimichthys polyactis) and the catch per one hour in each station ranged 7.7 to 182.5 kg/hour. 2. The mean volume backscattering strength for the entire water column and the bottom layer of the 0-10 m from bottom friction were -74.6 ㏈ and -68.2 ㏈, respectively. That is, the mean volume backscattering strength for the bottom layer was 6.4 ㏈ higher than that for the entire water column 3. In the surveys during the spring of 1997, the geographical distribution characteristics of fish schools suggests a trend of decreasing fish abundance toward the coast area of Korea and the highest demersal concentrations appeared in waters between the Cheju Island and the Tsushima Island 4. The distribution density of fish school in the East China Sea and Yellow Sea during the 1997 acoustic survey were estimated to be 6.65$\times$10$^{-5}$ kg/㎥ in the entire water column and 2.86$\times$l0$^{-4}$ kg/㎥ in the bottom layer, respectively.pectively.

• Journal of The Korean Society of Grassland and Forage Science
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• v.25 no.2
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• pp.119-124
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• 2005

This study was conducted from March 21 to July 8 in 2004 at JeJu Island to investigate the influences of split nitrogen application times on creeping bentgrass vegetation. Nitrogen rate was 20 kg/10a and it was applied from once to 5 times. The result obtained were summarized as follows; plant height was getting longer as nitrogen was split applied from once to 5 times. So it was longest at 5 times split nitrogen application, but it was no significance from 4 to 5 times. Root length, Minolta SPAD-502 chlorophyll meter reading value, leave and root weight n[e directly proportional plant height response. Degree of land cover Iud density of creeping bentgrass increased as nitrogen was split applied from once to 5 times. But degree of land cover md density of reed decreased. The number of reed species on decreased as nitrogen was split applied from once to 5 times. Then ranking of the dominant weeds were Portulaca oleracea Polygonum hydropiper and Stellaria media (at once split time nitrogen application), Portulaca oleracea, Polygonum hydropiper and Digitaria adscendens (at 2 split times nitrogen application), Digitaria adscendens and Portulaca oleracea Polygonum hydropiper, (at 3 split times nitrogen application), Portulaca oleracea, Poa annua and Polygonum hydropiper (at 4 md 5 split times nitrogen application). These results indicate that the optimum frequency of split N applications is four times for growth of creeping bentgrass in volcanic ash soils of Jeju island.

• Archives of Reconstructive Microsurgery
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• v.6 no.1
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• pp.96-102
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• 1997

The gracilis that is frequently used as a donor of free muscle trasfer is appropriate in the muscular shape and vascular position. This muscle is belonged to the second type of muscle group by the classification of the pattern of muscular nutrient vessel. The adductor branch or first perforating branch of deep femoral artery which supplies the proximal 1/3 of this muscle is a dominant one and this is used for the microscopic anastomosis of muscle or musculocutaneous flap. The minor vascular pedicles which enter the distal 1/3 of this of this muscle are branches of the superficial femoral artery and it is 0.5mm in diameter, 2cm in length with two venae comitantes. These minor pedicles supplies distal half of the gracilis muscle. This island musculocutaneous flap using distal vascular pedicle can be used to cover the defect of soft tissue around the distal femoral supra-condylar area, knee joint and proximal tibial condyle area which cause limitation of motion of knee joint, or in the cases that usual skin graft is impossible. The important operative procedure is as follows; The dissection is carried proximally and distally and the entire gracilis muscle including proximal and distal pedicle is completely dissected. After temporary blocking of the proximal vascular pedicle, the adequate muscle perfusion by the distal pedicle is identified and it is rotated to the recipient site around knee joint. The advantages of this procedure are simple, no need of microscopic vascular anastomoses and no significant functional loss of donor site. Especially in the cases of poor condition of the recipient vessel, this procedure can be used effectively. From 1991 to 1996, we performed 4 cases; complete survival of flap in 3 cases and partial survival of flap with partial necrosis in 1 case. This procedure is though to be useful in the small sized soft tissue defect of distal femoral supra-condylar area, knee joint and proximal tibial condylar area, especially in the defect of anterior aspect which expected to cause limitation of motion of knee joint due to scar contracture. But the problems of this procedure are the diameter of distal vascular pedicle is small and the location of distal vascular pedicle is not constant. To reduce the failure rate, identify the muscular perfusion of distal vascular pedicle after blocking the proximal pedicle, or strategic delay will be helpful.