• Korean Journal of Environmental Biology
• /
• v.26 no.1
• /
• pp.15-21
• /
• 2008

Ionizing radiation causes many alterations in photosynthetic machineries. However, there is no information about effects of ionizing radiation on the development of photosynthetic machineries in plants. We investigated the greening of etiolated mung bean seedlings after gamma-irradiation of 50 to 300 Gy. The irradiation inhibited seedling growth with great dependence on the radiation dose. In particular, growth of stems was more affected than that of hypocotyls. Irradiated leaves showed inhibition in growth, aberration in morphology, and yellowing in color depending on the radiation dose. Contents of photosynthetic pigments such as chlorophylls and carotenoids were significantly decreased in the irradiated leaves. The apparent electron transport rate for photosynthesis, ETR, was similarly changed depending on the radiation dose. However, the maximal photochemical efficiency of Photosystem II (PSII), Fv/Fm, was little affected by the irradiation. Moreover, the 50-Gy seedlings maintained the control level of light saturating for photosynthesis and showed slightly higher Fv/Fm values in spite of significant decreases in the photosynthetic pigment content and ETR. These results suggest that the inhibition of the overall photosynthetic capacity couldn’t be causally relatqaed with the repression in the initial development of irradiated seedlings and that the overall photosynthetic machineries can develop and work to some extent as a concerted system for photosynthesis even after exposure to acute doses of ionizing radiation.

• Korean Journal of Environmental Agriculture
• /
• v.19 no.1
• /
• pp.58-61
• /
• 2000

This study was conducted to determine the effect of low dose ${\gamma}-ray$ on the germination rate and physiology of germinative seeds of welsh onion ( Allicm fistulosum L. cv. Sukchangwoidae ) and spinach ( Spinacia oleracea L. cv. Chungrok ). The germination rate of irradiation group was much higher than that of the control. Especially it was noticeably higher in 1 or 2 Gy irradiation groups in the sowing spinach seeds on paper towel. On the welsh onion, the germination rate of the 1 Gy irradiation group increased by 17% compared to that of the control. Ion leakage from seeds irradiated with low dose of ${\gamma}-ray$ was decreased compared to that from the control especially at the early stage of incubation when examined by means of electric conductance. This tendency was confirmed in seeds of welsh onion and spinach. Starch hydrolysis was stimulated by ${\gamma}-ray$ irradiation in welsh onion. Furthermore ${\gamma}-ray$ irradiation was beneficial to keeping the vitality of seeds as determined through decarboxylation of glutamic acid.

• Food Science and Biotechnology
• /
• v.16 no.2
• /
• pp.315-319
• /
• 2007

Biofilm formation on various surfaces is a well-known phenomenon and it has caused pollution problems, health and safety hazards, and substantial economic loss in many areas including the food industry. In the present study, Gamma irradiation at a dose of 2.0 kGy reduced the bacterial counts of Staphylococcus aureus and Pseudomonas aeruginosa suspensions by 6.7 and >6.5 log CFU/mL, respectively, and 30 ppm of sodium hypochlorite effectively reduced the counts of both bacterial suspensions to below the limit of detection ($<2\;log\;CFU/cm^2$). However, in bacterial biofilms attached to stainless steel, gamma irradiation at a dose of 10.0 kGy reduced the counts of S. aureus attached fur 1 hr and overnight by ${\geq}5.1\;and\;5.0\;log\;CFU/cm^2$, respectively. Gamma irradiation at a dose of 1.0 kGy reduced the counts of P. aeruginosa counts to below the limit of detection ($<2\;log\;CFU/cm^2$). On the contrary, S. aureus and P. aeruginosa cells attached to stainless steel chips were difficult to eliminate using sodium hypochlorite. Four hundred ppm of sodium hypochlorite reduced the counts of S. aureus and P. aeruginosa attached for 1 hr by 2.5 and $3.3\;log\;CFU/cm^2$, respectively.

• Polymer(Korea)
• /
• v.26 no.6
• /
• pp.792-802
• /
• 2002

In this study, hydrogels from mixtures of poly (vinyl alcohol) (PVA)/poly(N-vinylpyrrolidone) (PVP)/glycerin/chitosan were prepared by γ-ray irradiation and the mechanical properties such as gelation, water absorptivity, and gel strength were examined to evaluate the applicability of these for wound dressing. Then PVA:PVP was weight ratio of 6 : 4, the concentration of chitosan was 0.3 wt%, the concentration of glycerin was in the range of 0∼5 wt%t. The solid concentration of PVA/PVP/glycerin/chitosan solution was 15 wt%. Gamma irradiation doses of 25, 35, 50, and 60 kGy were exposed to a mixture of PVA/PVP/glycerin/chitosan to evaluate the effect of irradiation dose. Gel content and gel strength increased as glycerin concentration in PVA/PVP/glycerin/chitosan decreased, and as irradiation dose increased. Swelling degree increased as glycerin concentration in PVA/PVP/glycerin/chitosan increased, and as irradiation dose decreased. The glycerin in PVA/PVP/glycerini/chitosan hydrogel prevented the transformation of shape. These hydrogel dressings had better curing effect than vaseline gauge.

• Korean Journal of Food Science and Technology
• /
• v.32 no.5
• /
• pp.1102-1106
• /
• 2000

This study was carried out to investigate the effects of gamma irradiation for the improvement of hygienic quality and the extension of shelf-life of Kwamegi(semi-dried colobabis seira). Kwamegi was stored at $5^{\circ}C$ and $15^{\circ}C$ after gamma irradiation with doses of 0, 3, 5, 7 and 10 kGy. In microbiological aspects, non-irradiated Kwamegi was rapidly deteriorated during storage, and some harmful bacteria were detected in a microbial analysis using a selective medium. However, the total viable cells and presumptive pathogens were reduced with the increase of irradiation dose, and dose level of 7 to 10 kGy was considered to be optimum and effective dose for the preservation of Kwamegi.

• Korean Journal of Food Science and Technology
• /
• v.16 no.3
• /
• pp.319-321
• /
• 1984

Black pepper powder was irradiated at dose of 3,6 and 9 kGy, stored at ambient temperature in order to investigate the effect of irradiation on their microbial sterilization and physicochemical properties. Total and coliform bacteria were sterilized by irradiation up to 9 kGy and 3 kGy, respectively, and they were not grown until the end of storage period. $D_{10}$ value of total bacteria were in the range of 1.39-1.49 kGy. Proximate composition was not significantly changed according to the increase of irradiation dose except slight increase of the reducing sugar. The yield of volatile oil was slightly decreased by the increase of irradiation dose.

• Journal of Radiation Industry
• /
• v.6 no.3
• /
• pp.211-215
• /
• 2012

In this study, crosslinked polyethylene (PE) foam sheets were prepared through gamma-ray irradiation. PE foam sheets were prepared through a foaming process using sodium bicarbonate as a blowing and foaming agent. The prepared PE foam sheets were then crosslinked through gamma-ray irradiation. The crosslinking degree was increased to 86.0% with an increase in the absorption dose. The tensile strength of the crosslinked PE foam sheets was increased with an increase in the absorption dose. However, the elongation-at-break of the crosslinked PE foam sheets was decreased. The thermal decomposition temperature of the crosslinked PE foam sheets was increased to $421.2^{\circ}C$ with an increase in the absorption dose. The SEM analysis revealed that the morphology was not changed significantly after the crosslinking through gamma-ray irradiation.

• Nuclear Engineering and Technology
• /
• v.51 no.5
• /
• pp.1428-1435
• /
• 2019

The total ionizing dose (TID) and non ionizing energy loss (NIEL) effects of 100 MeV phosphorous ($P^{7+}$) and 80 MeV nitrogen ($N^{6+}$) ions on 200 GHz silicon-germanium heterojunction bipolar transistors (SiGe HBTs) were examined in the total dose range from 1 to 100 Mrad(Si). The in-situ I-V characteristics like Gummel characteristics, excess base current (${\Delta}I_B$), net oxide trapped charge ($N_{OX}$), current gain ($h_{FE}$), avalanche multiplication (M-1), neutral base recombination (NBR) and output characteristics ($I_C-V_{CE}$) were analysed before and after irradiation. The significant degradation in device parameters was observed after $100MeV\;P^{7+}$ and $80MeV\;N^{6+}$ ion irradiation. The $100MeV\;P^{7+}$ ions create more damage in the SiGe HBT structure and in turn degrade the electrical characteristics of SiGe HBTs more when compared to $80MeV\;N^{6+}$. The SiGe HBTs irradiated up to 100 Mrad of total dose were annealed from $50^{\circ}C$ to $400^{\circ}C$ in different steps for 30 min duration in order to study the recovery of electrical characteristics. The recovery factors (RFs) are employed to analyse the contribution of room temperature and isochronal annealing in total recovery.

• Journal of Radiation Protection and Research
• /
• v.29 no.1
• /
• pp.9-15
• /
• 2004

Firstly, we compared the two staining techniques, Giemsa and Acridine orange, to determine micronuclei on samples of cultures of five healthy human peripheral blood lymphocytes after ${\gamma}-irradiation\;(^{137}Cs)$ in dose ranges of 0 to 800cGy. It was found that the Acridine orange staining method gives more reliable results than the usual Giemsa staining method in micronucleus tests. Moreover, the frequency of micronuclei in cytokinesis-blocked human B-lymphocytes was studied after in vitro irradiation in dose ranges of 0 to 50cGy. After setting and separating the B-lymphocytes, the frequency of radiation-induced micronuclei were observed as the end-point markers for the low-dose radiation dosimetry after staining with Giemsa and Acridine orange dyes. The micronuclei frequency in B-lymphocytes was significantly elevated from 10 to 30cGy ${\gamma}-irradiation$. The determination of micronuclei in B-lymphocytes after staining with Acridine orange was higher than that of Giemsa. The frequency of micronuclei in B-lymphocytes was observed to be at least two times higher than those of T-lymphocytes Giemsa in dose increasing. Therefore, the determination of low-dose radiation-induced micronuclei in B-lymphocytes after staining with Acridine orange is likely to have the greatest potential in the estimation of low dose radiation exposure.

• Korean Journal of Environmental Agriculture
• /
• v.20 no.2
• /
• pp.116-121
• /
• 2001

To observe the stimulating effects of low dose ${\gamma}$ - radiation on the dormancy breaking and physiological activity, potato (Solanum tuberosum L. cv. Dejima) were irradiated at the dose of $0.5{\sim}30$ Gy. Low dose ${\gamma}$ - radiation exhibited promoting effects on the sprouting rate within the range of optimum dose of 1 Gy and 2 Gy and on the sprout length within the range of optimum dose of 2 Gy and 4 Gy. Regardless of storage duration and irradiation doses, the number of sprouts was enhanced. The number of sprouts, which were reserved for 15 days after production (DAP), were significantly increased under 4, 8 and 16 Gy irradiation. The growth of 45 DAP sprouts was extremely stimulated under 4 Gy irradiation accompanying the increase of peroxidase activity in the plantlet. In this study, it was also suggested that the activities of antioxidative enzymes of potato plantlets were not related to the irradiation dose during the plantlet development with the exception of decrease in catalase activity.