• Journal of the Korean Society of Food Science and Nutrition
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• v.28 no.3
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• pp.698-704
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• 1999

In this study, we evaluated the radioprotective effects of Kuei Pi Tang and its ingredients, as a prescription of traditional Oriental medicine. The jejunal crypt survival, endogenous spleen colony formation, and apoptosis in jejunal crypt cells were investigated in mice irradiated with high and low dose of ${\gamma}$ rays. In the mice administered with Kuei Pi Tang extract before irradiation, the jejunal crypts were protected significantly(p<0.005) and the frequency of radiation induced apoptosis was reduced(p<0.05). In the experiment on the effects of the ingredients of Kuei Pi Tang, the results indicated that the extract of Dangui(Angelica sinensis), Suanzaoren(Zizyphus valgaris), Rensan (Panax ginseng), Fuling(Poria cocos) and Muxiang(Saussurea lappa) might have a major radioprotective effect. Although the mechanisms of this inhibitory effect remain to be elucidated, these results indi cated that Kuei Pi Tang might be a useful radioprotector, especially since it is a relatively nontoxic natural product.

• Korean Journal of Environmental Biology
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• v.25 no.2
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• pp.107-114
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• 2007

Radiation at very low doses frequently has a stimulating or hermetic effect on growth of organism. Effects of growth and survival rate on various dose of gamma irradiation in the farm culture of juvenile abalone (Haliotis discus hannai) were determined in Hallim (Jeju) from February 2004 to January 2005. The initial shell length of abalone juveniles in this study was average $3.45{\pm}0.4cm$. A change of growth after irradiation $(0{\sim}20Gy)$ was observed for 48 weeks. The highest growth rate was observed in 4 Gy-irradiated group and the lowest growth rate was observed in 20 Gy-irradiated group. The additional research about biochemical changes on juvenile abalone after irradiation should be accomplished. Continuous study for gamma radiation-induced hormesis on growth and metabolism of juvenile abalone will further induce the creation of value in ocean industry.

• Food Science and Biotechnology
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• v.17 no.4
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• pp.751-754
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• 2008

Texture analysis, sensory evaluation, and scanning electronic microscopic (SEM) observation were conducted to evaluate the effects of different calcium salts (calcium lactate, calcium acetate, and calcium chloride) and vitamin C on the textural properties of kimchi, gamma-irradiated at 25 kGy. Increase of the hardness and sensory score were observed in the kimchi pretreated with calcium salt or vitamin C as compared with the untreated and irradiated kimchi. And the hardness and sensory quality of the co-pretreated sample with 0.01% of calcium lactate and 0.3% of vitamin C were the highest after 30 days at $35^{\circ}C$, which indicates that the co-pretreatment is effective in preventing a decrease of the texture and sensory qualities of kimchi by gamma irradiation. Also, this result was supported by the SEM observation.

• Current Research on Agriculture and Life Sciences
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• v.4
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• pp.65-69
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• 1986

The radurization and radicidation effects on mixed condiment with Co-60 gamma irradiation at 3-9kGy and the physicochemical aspects of irradiated samples stored at $30^{\circ}C$ for three months were investigated. The initial microbial loads of the sample were $7.5{\times}10^5/g$ in total bacterial count and $1.2{\times}10^2/g$ in coliforms, respectively. The irradiation treatment of below 9 kGy could decontaminate the sample and $D_{10}$ value of the bacteria contaminated was shown to be 1.94 kGy. The chemical components associated with the quality of the sample were little affected by the irradiation at 3-6kGy. The sensory evaluation showed that the high sterilizing dose caused appreciably the change in overall flavors of mixed condiment.

• Journal of the Korean Society of Food Science and Nutrition
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• v.30 no.5
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• pp.854-857
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• 2001

Electron spin resonance (ESR) spectroscopy was used to detect free radicals in irradiated dried vegetables. Dried carrot, mushroom and green onion were irradiated with doses of 0, 1, 3, 5 and 7 kGy at room temperature using a Co-60 irradiator. Free radicals were detected in irradiated dried carrot, green onion and mushroom, while those were not detected in non-irradiated samples. Since concentration of free radicals linearly increased with applied doses (1~7 kGy), highly positive correlation coefficients ($R^2$=0.9747~0.9919) were obtained between irradiation doses and free radical concentrations right after irradiation. Although concentration of free radicals slowly decreased with storage time, the characteristic ESR signals of free radicals slowly decreased with storage time, the characteristic ESR signals of free radical in irradiated samples were observed even after 3 months of storage at room temperature.

• Preventive Nutrition and Food Science
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• v.6 no.4
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• pp.211-215
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• 2001

The packaging effect on physicochemical changes in irradiated sausage stored at -2$0^{\circ}C$ was studied. Emulsion-type cooked pork sausage was made with (156 ppm) or without NaNO$_2$ (0 ppm), and packaged in three different conditions such as aerobic, vacuum and $CO_2$ (100%). The samples irradiated at 0, 5 and 10 kGy absorbed dose, and the total number of microorganisms, lipid oxidation, color and texture were analyzed during frozen storage at-2$0^{\circ}C$. Irradiation of the sausage at 10 kGy completely controlled microbial growth during storage. An NaNO$_2$ addition to the sausage significantly reduced lipid oxidation, and the TBARS value of the sausage with aerobic packaging was higher than that with the vacuum and $CO_2$ packaging. The NaNO$_2$ addition increased Hunter color a-value dramatically, but no packaging effect was found (p > 0.05). Irradiation influenced shear values resulting in lower shear values in 10 kGy-irradiated sausages with aerobic packaging, and $CO_2$ packaged sausage showed comparatively lower shear value than other packaging methods. From the results, vacuum or $CO_2$ (100%) packaging were better than aerobic packaging for frozen stored pork sausage, especially far microbial quality and lipid oxidation.

• Journal of Radiation Industry
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• v.5 no.1
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• pp.35-39
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• 2011

This study was investigated the effect of tumor cell cytotoxicity of gamma irradiated Chaga mushroom extract (CME). CME was prepared by hot water extraction at $70^{\circ}C$ for 4 hours and lyophilized. $Ten\;mg\;ml^{-1}$ of lyophilized CME powder was dissolved with deionized water and then irradiated at the doses of 10, 50, 100, and 150kGy by cobalt 60 gamma irradiator. The gamma-irradiated and non-irradiated CME were treated into the cancer cell, including human stomach cancer and human colon cancer. Cytotoxicity against the cancer cell was increased in gamma-irradiated CME and antioxidant activity was also increased in gamma-irradiated CME, as irradiation dose increased. Therefore, it was considered that gamma irradiation was effective method for improvement of the cancer cell cytotoxicity and antioxidant activity of Chaga mushroom extract.

• Journal of Radiation Industry
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• v.6 no.2
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• pp.139-145
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• 2012

Gold nanoparticles (GNPs) have led to the development of a new field in the diagnosis and treatment of diseases such as cancer. An efficient synthesis of gold nanoparticles within the range of 8~57 nm was established by ${\gamma}-ray$ irradiation. The good point of a radiation-based method is the production of gold nanoparticles with a higher concentration and narrower size distribution compared with conventional methods. The size of gold nanoparticles was controlled using two methods. : (i) varying the ${\gamma}-ray$ irradiation dose of 10 to 25 kGy and (ii) varying the concentration of $HAuCl_4$ solution from 4 to 40 mM. In addition, the GNPs were radiolabeled using $[^{125}I]NaI$ in a simple and fast manner with high yields. The produced gold nanoparticles were characterized using a transmission electron microscopy (TEM), a UV-visible spectrophotometer, and a radio-TLC imaging scanner. From these results, these radiolabeled GNPs can be applicable for a radioisotope tag of biomolecules.

• Journal of Ginseng Research
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• v.37 no.4
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• pp.483-490
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• 2013

Gamma-irradiation (0-7 kGy) of ginseng is permitted in Korea for the purpose of microbial decontamination; with strict labeling, traceability and monitoring requirements. An identification study was conducted to determine the photostimulated-luminescence (PSL) and thermoluminescence (TL) properties of gamma-irradiated fresh and white ginsengs cultivated in different areas. Dose- dependent PSL-based screening was possible for white ginseng samples; however, inappropriate results from non-irradiated fresh ginseng samples were obtained, showing intermediate (700 to 5,000) or positive ($T_2$ >5,000, irradiated) PSL counts due to the abundance of minerals on the surfaces of the samples. TL analysis of separated minerals from all non-irradiated samples gave TL glow curves of low intensity with a maximum peak after $300^{\circ}C$. However, well-defined irradiation-specific (high intensity with a maximum peak at about $200^{\circ}C$) glow curves were observed for all the irradiated samples, regardless of their type and origins. TL ratios (first glow curve /second glow curve) were also determined to confirm the irradiated (>0.1) and non-irradiated (<0.1) results. SEM-EDX (scanning electron microscope-energy dispersive X-ray) and XRD (X-ray diffraction) spectroscopic analyses showed that feldspar and quartz minerals were the main source for the typical radiation-specific luminescence properties.

• Entomological Research
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• v.48 no.5
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• pp.331-338
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• 2018

Spotted wing drosophila (SWD) has emerged as a major invasive insect pest of small berry fruits in the Americas and Europe since the late 2000s. Thus, phytosanitary treatment of commodities for export is imperative to prevent the movement of viable SWD to newer areas. In the present study, all developmental stages of SWD were irradiated with different doses of gamma and electron beam radiation to assess developmental inhibition to identify potential quarantine doses of the radiations. Ionizing radiation induced developmental inhibition of all stages of SWD. The effective doses for 99% inhibition ($ED_{99}$) of hatching, pupariation, and adult emergence from irradiated eggs for gamma radiation were 882, 395 and 39 Gy, respectively, compared with 2849, 687, and 41 Gy, respectively, for electron beam radiation. The $ED_{99}$ for inhibition of pupariation and adult emergence in irradiated larvae were 703 and 47 Gy, respectively, for gamma radiation, and 619 and 33 Gy, respectively, for electron beam radiation. Pupal irradiation did not completely inhibit adult emergence, even at 300 Gy. However, irradiation with ${\geq}100Gy$ of puparia induced adult sterility, with no egg production at all. The $ED_{99}$ for inhibition of $F_1$ egg hatchability from adults irradiated with gamma radiation and electron beam radiation was estimated to be 424 and 125 Gy, respectively. The results of the present study suggest that gamma radiation and electron beam radiation are alternatives for phytosanitary treatment. Irradiation with 100 Gy could be suggested as a potential dose for egg, larval, and pupal quarantine treatment of SWD.