• Title/Summary/Keyword: Investigation of Origin

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Investigation of the Gene Encoding Isotocin and its Expression in Cinnamon Clownfish, Amphiprion melanopus (Cinnamon clownfish Amphiprion melnaopus의 이소토신 유전자 구조와 삼투압 조절이 미치는 영향)

  • Noh, Gyeong Eon;Choi, Mi-Jin;Min, Byung Hwa;Rho, Sum;Kim, Jong-Myoung
    • Journal of Life Science
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    • v.26 no.2
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    • pp.164-173
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    • 2016
  • Isotocin (IT), a nonapeptide homolog of oxytocin in mammals, has been suggested to be involved in physiological processes including social behaviors, stress responses, and osmoregulation in teleost fish. To study its structure and function, the gene encoding the IT precursor was cloned from the genomic DNA and brain cDNA of the cinnamon clownfish, Amphiprion melanopus. The IT precursor gene consists of three exons separated by two introns, and encodes an open reading frame of 156 amino acid (aa) residues, comprising a putative signal peptide of 19 aa, a mature IT protein of 9 aa, a proteolytic processing site of 3 aa, and 125 aa of neurophysin. Tissue-specific analysis of the IT precursor transcript indicated its expression in the brain and gonads of A. melanopus. To examine its osmoregulatory effects, the salinity of the seawater (34 ppt) used for rearing A. melanopus was lowered to 15 ppt. Histological analysis of the gills indicated the apparent disappearance of an apical crypt on the surface of the gill lamella of A. melanopus, as pavement cells covered the surface upon acclimation to the lower salinity. The level of Na+/K+-ATPase activity in the gills was increased during the initial stage of acclimation, followed by a decrease to its normal level, suggesting its involvement in osmoregulation and homeostasis. The only slight increase in the level of IT precursor transcript in the A. melanopus brain upon low-salinity acclimation suggested that IT played a minor role, if any, in the process of osmoregulation.

The Effects of Plum Extracts on the Proliferation of Human Epithelial Cell and Human Cervical Cancer Cells (자두 추출물이 인체 상피세포와 자궁경부암세포의 증식에 미치는 효과)

  • Han, Man-Deuk;Kweon, Dur-Han;Kang, Byung-Tae;Lee, Jae-Woo;Yoon, Ok-Hyun
    • Journal of the East Asian Society of Dietary Life
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    • v.17 no.5
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    • pp.710-718
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    • 2007
  • This study was designed to investigate the effects of plum(Prunus salicina Lindl. cultivars 'Oishiwase', 'Formosa', and 'Soldam') extracts on the proliferation as well as inhibition of human epithelial cells(HaCaT), human cervical carcinoma (HeLa, SiHa, and C33A) cells, and human stomach adenocarcinoma(SNU 638) cells. Dried plum was sequentially extracted and fractionated by hexane(KC-01), chloroform(KC-02), ethyl acetate(KC-03), n-butanol(KC-04), water(KC-05), methanol(KC-6), and hot water extract(KC-07). The epithelial and cancer cells were exposed for 48 h to $50{\mu}g/mL$ of plum extract in vitro, and were then analysed by a sulforhodamin B(SRB) staining assay. The methanol extract(KCP-6) of 'Formosa' proliferated not only the HaCaT cells(147.3%), but also the cervical carcinoma C33A cells(167.8%). The ethyl acetate extract of 'Soldam'(KCJ-3) significantly reduced the proliferation rate of the HPV positive conical carcinoma cells, at 61.5% for the SiHa cells and 70.5% for the HeLa cells. In the C33A cells, which are HPV negative cervical carcinoma cells, the hexane fractions of 'Formosa'(KCP-1) and 'Oishiwase'(KCD-1) markedly suppressed proliferation activity at 20.4% and 61.7%, respectively. However, the proliferation rate of the normal epithelial cells(HaCaT cell) was not reduced the proliferation rate by KCJ-3, KCP-1, or KCD-1, There were no significant effects on proliferation of the stomach cancer cells(SNU 638) by any of the extracts or fractions of the plum cultivars. These results suggest that the anti-proliferative effects of the plum cultivars were selective to the cancer cell origin. In conclusion, we found that several plum cultivar extracts, especially, the ethyl acetate fraction of 'Soldam" and the hexane fraction of "Formosa', have anti-proliferative activity toward human cervical carcinoma cells. However, further investigation is needed to assess the molecular mechanisms that mediate the antiproliferation activities of the plum cultivars.

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Study of Characteristics for Red Adhesive in Traditional Gold Thread (전통 편금사에 사용된 붉은 접착제 특성 연구)

  • Kim, Ji Eun;Yu, Ji A;Han, Ye Bin;Chung, Yong Jae
    • Journal of Conservation Science
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    • v.32 no.1
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    • pp.43-49
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    • 2016
  • Gold threads mean thread made by metal, it is composed various material to gold leaf, adhesive and media. The making technique and using materials of Gold threads are different from region, it can be used to distinguish country of origin. So this study finds characteristics and making techniques for flat gold threads of relics. Standard samples are made of animal glue, lacquer, red lacquer and etc. This materials are based on the scientific investigation of cultural gold threads properties. Then traditional techniques were estimated by comparison and analysis of actual cultural properties and standard samples. As a result of investigating cultural properties, it is similar to the standard sample which are mixed with red soil additives. After analysis of SEM-EDS, it is detected to Al, Si, Fe. So it is to the conclusion that traditional gold thread is made of adhesive mixed with red soil to improve gold color better. And this ways are similar with china that uses animal glue and red soil to adhesive and different from japan that uses lacquer to adhesive. Through this study, it will be used to data and reference for reappearance and making techniques of traditional gold threads.

Investigation on Sorting Efficiency for Recyclable Materials and Its Improvement Measure at Domestic Sorting Facility (국내 재활용품 선별시설에서 선별 현황 및 개선방안)

  • Kim, Joo-Sin;Pak, Daewon
    • Journal of the Korea Organic Resources Recycling Association
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    • v.25 no.2
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    • pp.15-26
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    • 2017
  • This study was conducted to investigate and analyze the discharge characteristics of recyclable material from S-city, S-district, in order to improve the sorting efficiency in recycling sorting facility. The characteristics of recyclable materials collected were analyzed in three different scopes; source origin, collection and transportation, and sorting steps. The average of recyclable waste generation is $0.121kg/day^*man$. Regional collection period appears to be three times a week, and the density of mixed recyclable wastes showed the average of $202.4kg/m^3$ in the waste collection vehicle. In the analysis into the sorting steps, the average of carrying amount of mixed recycling products is 1,154.6 ton/month, the average of appeared density is $181kg/m^3$, the average amount of separated recycling products is 448.5kg/month, and the density of recycling residue is found out to be $48kg/m^3$. The sorting rate of recyclable material is 38.85% and the percentage of residues is 55.90%. Out of 7,744.8 tons of the total recyclable residues, 4,272.1 tons were found out to be possible recylable materials. As a result of increasing the recycling rate of residues, the encouragement of base-recycling, the automation and retrofit of sorting equipment, and energy recovery from recycling residue were discussed.

Occurrence and Applied-mineralogical Characterization of Diatomite from the Pohang-Gampo Area (포항-감포 지역산 규조토의 산출상태와 응용광물학적 특성)

  • Noh, Jin-Hwan
    • Journal of the Mineralogical Society of Korea
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    • v.19 no.4 s.50
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    • pp.311-324
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    • 2006
  • In the Pohang-Gampo area, several diatomite beds occurred in mostly thinner than 1 m are embedded in the Pohang Formation of marine environment and the pyroclastic Eoil Formation. The diatomite from the Eoil Formation is constituting the high-grade ore altered slightly by diagenesis. In contrast, the diatomite intercalated within the upper horizon of the Yeonil Group is comparatively low-grade and highly altered in places. During diagenesis, an increasing of crystallinity of opal, i.e., the original mineral component of diatom, results in ultimately the mineral transition to quartz with accompanying a drastic change in morphology and texture of the altered diatomite. The diagenetic alteration appears to have undergone by way of the chemical diagenesis, which is largely controlled by degree of fluid contact, rather than burial diagenesis. For the diatomite from the Pohang-Gampo area, careful SEM observations, XRD, chemical analyses, and determination of specific surface area were done to identify the fossil species, mineral and chemical composition, and other physical properties in the view of assesment of grade and quality. The domestic diatomite ores are evaluated to be not good in grade and quality, compared to those of famous foreign localities. However, some diatomite deposits of marin,: origin from the Pohang Formation is constituting a peculiar clay-rich type, i.e., moler applicable to the special usage such as a manufacturing of lightweight brick. Because such a diatomite is frequently intercalated relatively as a thicker bed in the upper part of the Yeonil Group, a systematic and careful investigation should be done for the exploitation and development of an economic diatomite deposit of the moler type.

Current Status and Characteristics of Highly Pathogenic Avian Influenza (고병원성 가금인플루엔자의 최근 발생동향과 질병 특성)

  • Kim, J.H.;Sung, H.W.;Kwon, Y.K.;Lee, Y.J.;Choi, J.G.;Cho, S.J.;Kim, M.C.;Lee, E.K.;Jang, H.;Wee, S.H.;Mo, I.P.;Song, C.S.;Park, J.M.
    • Korean Journal of Poultry Science
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    • v.31 no.2
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    • pp.119-128
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    • 2004
  • Highly Pathogenic Avian Influenza (HPAI) is a very acute systemic disease in poultry, particularly in chickens and turkeys caused by HPAI viruses. An outbreak of HPAI caused by subtype H5N1, was first reported in a broiler breeder farm on December 10, 2003 in Korea, although there had been twenty one outbreaks of the disease reported in the world before. Since mid-December 2003, eight Asian countries have confirmed outbreaks of HPAI due to the same subtype. The outbreak has also resulted in at least twenty three fatal human cases in Vietnam and Thailand as of May 17, 2004 according to the WHO. Regarding the first outbreak of recent Asian HPAI, it has been suspected that some Asian countries with the exception of Korea and Japan veiled the fact of HPAI outbreaks since the last half of 2003, even though it was first reported in Korea. There have been total nineteen outbreaks of HPAI among chicken and duck farms in 10 provinces in Korea since Dec. 2003 and approximately 5,280,000 birds were slaughtered from 392 farms for eradication of the disease and preemptive culling. The origin of the H5Nl HPAI virus introduced into the country are unknown and still under epidemiological investigation. Current status of outbreaks and characteristics of HPAI will be reviewed and discussed on the basis of genetic, virological, clinicopathological, and ecological aspect, as well as future measures for surveillance and prevention of the disease in Korea.

An Analysis of Street Fashion in Northeast 3-Sung, China(Part I) -Focusing on 2006 Spring in Dalian- (중국 동북 3성 스트리트 패션 분석(제1보) -2006년 봄 대련시를 중심으로-)

  • Bae, Soo-Jeong
    • Journal of the Korean Society of Clothing and Textiles
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    • v.31 no.11
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    • pp.1554-1564
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    • 2007
  • In apparel industry, the production of clothing for the global market, has it#s origin in its characteristics depending on hands on working style, according to the globalization of a fashion industry. These days, however, the globalized production is unable to keep pace with the short cycle of production due to the unpredictable change of the taste or demands in the local area, as a result, the industry has come to take a focus on the production and consumption in the circumscribed region. In this stream, the northeastern area, specially, Dalian which is renowned for a center of fashion was designated as a city of this study as a representative. The cultural, racial and geographical uniqueness, asks for the analysis, based on the individual local area on the part of Korean fashion company desiring to launch into China fashion market. The purpose of this thesis is to analyse the style, color, and items of street fashion in Dalian. I would contribute to the understanding the preference of fashion in northeastern area, thereby, affording a fundamental resources for designing strategies for Korean fashion brands in China. The period of investigation is about 5 weeks from 14 May 2006 until 18 June 2006, with combined use of camcorder and digital camera. The site was Victory Shopping Plaza, in center of city, also with Mycal, Ansung, Dasang department store situated in the Economic Development area. The result of this study are as follows: 1. Preferred clothing styles are casual styles(68%) rather than formal styles(32%). In the casual styles, sports casual(36.0%), character casual(29.5%), jean casual(2.6%) were listed. All kinds of clothes are tightly fitted due to the stretched fabric and knit fabric. 2. Preferred colors are white(31.1%), red(16.6%), black(15.8%) khaki(7.8%) and blue(7.6%) for tops, and black(34.6%), blue(27.2%), white(15.4%), brown(14.6%) for bottoms. 3. Preferred clothing items are T-shirt(36.5%), jumper(33.6%), jacket(20.0%), blouse(8.7%), etc.(1.2%) for tops, pants(91.4%), skirts(8.6%) for bottoms. In the pants, cigarette pants(34.6%), bell bottom pants(21.0%), cargo pants(19.9%), straight pants(15.9%) were listed respectively. In the skirts, flare skirt(3.9%), tight skirt(2.2%), semi tight skirt(1.7%), pleats skirt(0.8%) were listed.

Migration Characteristic Analysis on Red Tide Using GIS (지리정보시스템을 이용한 적조의 이동특성분석)

  • Kim, Jin-Gi
    • Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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    • v.25 no.3
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    • pp.257-266
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    • 2007
  • The research on red tide is generally in progress through field work, such as the naked eye and sampling. It was difficult to forecast exactly the course, from appearance of red tide to disappearance. with the established ways of investigation and analysis. Accordingly it is need to analyze environmental factors in time and space, the appearance of red tide and the path of its migration by more objective and scientific methods. In this study, GIS is applied to analyse the space character of red tide and the interpolation of IDW(Inverse Distance Weight) is applied to assume the density distribution of red tide after gather data by using Arc/Info. After IDW interpolation, the sea area occurred over 1,000 cells/ml of red tide density is extracted with CON and SUM Function of Grid Module, and the density of the sea area is accumulated daily. As a result of this study, the distribution condition of red tide is found timely and spacially by applying GIS to the sea area of red tide, the results indicated that the spatial density and the cumulative frequency about the origin of red tide using GIS, the sea area demonstrated that the maximum density and the maximum frequency varied significantly over the Nammyun of Namhae-Is. with the maximum frequency being 49 times. accordingly if data about the areas of red tide will occur from the present are accumulated, the shifting route of red tide occurrence and extinction can be predicted.

Investigation of the influence of substrate surface on the ZnO nanostructures growth (기판 표면의 영향에 의한 ZnO 나노 구조 성장에 관한 연구)

  • Ha, Seon-Yeo;Jung, Mi-Na;Park, Seung-Hwan;Yang, Min;Kim, Hong-Seung;Lee, Uk-Hyeon;Yao, Takafumi;Jang, Ji-Ho
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • v.9 no.1
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    • pp.1022-1025
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    • 2005
  • The effect of substrate surface to the formation of ZnO nanostructures has been investigated using Si (111), $Al_2O_3$(C-plane) $Al_2O_3$(A-plane), and $Al_2O_3$(R-plane) substrates. The growth temperature was controlled from 500$^{\circ}C$ ${\sim}$ 600$^{\circ}C$, and the luminescence properties were investigated by a series of photoluminescence (PL) measurements at the elevating temperatures. ZnO nanostructures grown on Si substrate show strong UV emission intensity along with green emission positioned at 3.22 eV and 2.5 eV, respectively. However, green emission was not observed from the ZnO nanostructures grown on $Al_2O_3$ substrates. It is explained in terms of the difference of the surface energy between Si and $Al_2O_3$. Also, the origin of UV emissions has been discussed by using the temperature-dependent PL. The distinction of the PL spectra is interpreted in terms of the difference of the impurity included in the nanostructures.

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A Study on the Forming and Evolution of Coastal Flood Origin Deposits at Gwangseungri Coast - Based on Burial Age and Chemical Analysis - (광승리 연안의 연안범람기원퇴적층 형성과 변화 과정에 대한 연구 - 퇴적물의 매몰연대와 화학분석을 기반으로 -)

  • Shin, Won Jeong;Yang, Dong Yoon;Kim, Jong Yeon
    • Journal of The Geomorphological Association of Korea
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    • v.25 no.3
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    • pp.71-87
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    • 2018
  • As part of further study on Gwangseungri coastal deposits which occurred at 10 ~ 15m above sea level and was analyzed as palaeo-coastal flood-type sediments, six burial ages of six additional samples from the two cross sections (KST1 and, KST2) near to the points of the past study were estimated and the geochemical analysis was performed. Further investigation on the cross section KST1 revealed a reversal of the burial age at the bottom of the section which was identified as palaeo-flooding sediments and supposed to have been buried about 350 years ago. At the lower part of the KST1, the burial age of the sediment layer was estimated to be 3,800 years. The lower part of KST2 sediments was identified as sediments that was formed about 6,600 years ago and about 20,000 years ago. Considering the inclination of the sediment layers, the coastal flooding sedimentsreported to have formed 700 years ago in the previousstudy are located at the top and the KST1 section analyzed in thisstudy seemed to be connected to the lower part. The chemical analysis showed that the relationship between these layers was not continuous but had a discontinuous characteristic influenced by a specific event, and the chemical composition also showed a rapid change. If we judge these together, the lowest part of Gwangseungrisediment layerseemed to have formed during the last glacial period but it was hard to find its origins clearly. On top of this layer, a fine sediment layer containing gravels was also formed.Itseemed thatsedimentation did not occur continuously, but was affected by temporary events in such a way that after a sediment layer was formed, it stopped. Since then, a coastal flooding event occurred about 700 years ago, and part of flooded sediments accumulated in the rear slope. After that, when a flood layer including additional granular materials about 350 years ago was formed, sedimentation along the slope seemed to have occurred.