• ALGAE
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• v.22 no.2
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• pp.95-106
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• 2007

Blooms of Chattonella species are normally during summer in inland seas with high nutrients from the land and inflowing water. These blooms cause mass fish kills worldwide. We isolated a Chattonella strain from the south coast of Korea and identified it as C. antiqua. It is known that the morphological changes of phytoplankton correspond to the diurnal vertical migrations that follow an intrinsic biological clock and a nutrient acquisition mechanism during the day and night. In electron micrographs, C. antiqua clearly showed a radial distribution of lipid bodies in subcellular regions and plastids composed in which thylakoid layers were perpendicular to the surface. A single pyrenoid was present in each plastid and it was found at the end of the plastid towards the center of the cell. Throughout the day, plastids of C. antiqua cells appeared as an expanded net-like recticulum. During the night, however, the plastids changed their shape and contracted toward the cell periphery. The electron density of pyrenoids was increased in cells harvested during the night.

• Proceedings of the Korean Vacuum Society Conference
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• 2014.02a
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• pp.229.1-229.1
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• 2014

Tactile sensors have widely been researched in the areas of electronics, robotic system and medical tools for extending to the form of bio inspired devices that generate feeling of touch mimicking those of humans. Recent efforts in adapting the tactile sensor have included the use of novel materials with both scalability and high sensitivity [1]. Graphene, a 2-D allotrope of carbon, is a prospective candidate for sensor technology, having strong mechanical properties [2] and flexibility, including recovery from mechanical stress. In addition, its truly 2-D nature allows the formation of continuous films that are intrinsically useful for realizing sensing functions. However, very few investigations have been carrier out to investigate sensing characteristics as a device form with the graphene subjected to strain/stress and pressure effects. In this study, we present a sensor of vertical forces based on single-layer graphene, with a working range that corresponds to the pressure of a gentle touch that can be perceived by humans. In spite of the low gauge factor that arises from the intrinsic electromechanical character of single-layer graphene, we achieve a resistance variation of about 30% in response to an applied vertical pressure of 5 kPa by introducing a pressure-amplifying structure in the sensor. In addition, we demonstrate a method to enhance the sensitivity of the sensor by applying resistive single-layer graphene.

• Transactions of Materials Processing
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• v.21 no.4
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• pp.240-245
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• 2012

With the increasing demand for light-weight materials to reduce fuel consumption, the automobile industry has extensively studied magnesium alloys which are light weight metals. The intrinsic poor formability and poor ductility at ambient temperature due to the hexagonal close-packed (HCP) crystal structure and the associated insufficient number of independent slip systems restricts the practical usage of these alloys. Hot working of magnesium alloys using a forging or extrusion enables net-shape manufacturing with enhanced formability and ductility since there are several operative non-basal slip systems in addition to basal slip plane, which increases the workability. In this research, the thermomechanical properties of AZ80 Mg alloy were obtained by compression testing at the various temperatures and strain rates. Optical microscopy and EBSD were used to study the microstructural behavior such as misorientation distribution and dynamic recrystallization. The results were correlated to the hardening and the softening of the alloy. The experimental data in conjunction with a physical explanation provide the optimal conditions for net-shape forging under hot or warm temperatures through control of the grain refinement and the working conditions.

• Structural Monitoring and Maintenance
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• v.8 no.4
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• pp.379-402
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• 2021

Traditional approaches for structural health monitoring (SHM) seldom take ambient uncertainty (temperature, humidity, ambient vibration) into consideration, while their impacts on structural responses are substantial, leading to a possibility of raising false alarms. A few predictors model-based approaches deal with these uncertainties through complex numerical models running online, rendering the SHM approach to be compute-intensive, slow, and sometimes not practical. Also, with model-based approaches, the imperative need for a precise understanding of the structure often poses a problem for not so well understood complex systems. The present study employs a data-based approach coupled with Empirical mode decomposition (EMD) to correlate recorded response time histories under varying temperature conditions to corresponding damage scenarios. EMD decomposes the response signal into a finite set of intrinsic mode functions (IMFs). A two-dimensional Convolutional Neural Network (2DCNN) is further trained to associate these IMFs to the respective damage cases. The use of IMFs in place of raw signals helps to reduce the impact of sensor noise while preserving the essential spatio-temporal information less-sensitive to thermal effects and thereby stands as a better damage-sensitive feature than the raw signal itself. The proposed algorithm is numerically tested on a single span bridge under varying temperature conditions for different damage severities. The dynamic strain is recorded as the response since they are frame-invariant and cheaper to install. The proposed algorithm has been observed to be damage sensitive as well as sufficiently robust against measurement noise.

• Microbiology and Biotechnology Letters
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• v.31 no.1
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• pp.63-68
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• 2003

Saccharomyces cerevisiae KNU5377 is a constitutively thermotolerant, fermentative strain at high temperatures over 4$0^{\circ}C$. The exposure to 0.5 M NaCl caused S. cerevisiae KNU5377 to be lost its constitutive thermotolerance. Furthermore, the NaCl adaptation beyond 0.3 M during the overnight culture forced the strain-specific fermentation ability of S. cerevisiae KNU5377 to be disappeared. However, these phenomena did not occur in the reference, Saccharomyces cerevisiae ATCC24858. As a result, this adaptation led both strains to show the closely similar thermotolerance level and alcohol fermentation ability, implying the NaCl adaptation eliminated its strain-specific characteristics of S. cerevisiae KNU5377 Therefore it indicated that the superior intrinsic characteristics of S. cerevisiae KNU5377 must be related to the NaCl adaptation. On the other hand, the heat adaptation elevated alcohol productivity for both strains, but surprisingly did it for KNU5377 at the rate of two times higher than the reference's one; this suggests that KNU5377 possesses more efficient system enough to cause the difference. Consequently, these characteristics of S. cerevisiae KNU5377 must be interesting targets for further study to understand on how KNU5377 could acquire the constitutive thermotolerance and the outstanding fermentative capacity at high temperatures.

• Computers and Concrete
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• v.2 no.3
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• pp.203-214
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• 2005

In the Part 1 paper (Gawin, et al. 2005) some experimental results concerning micro-structural tests, permeability measurements and stress-strain tests of four types of High Performance Concrete, exposed to elevated temperatures (up to $700^{\circ}C$) are presented and discussed. On the basis of these experimental results parameters of the constitutive relationships describing influence of damage and temperature upon material intrinsic permeability at high temperature were determined. In this paper the effects of various formulations of damage-permeability coupling on results of computer simulations are analysed and compared with the results obtained by means of the previously proposed approach, that does not take into account the thermo-chemical concrete damage directly. Numerical solutions are obtained using the recently developed fully coupled model of hygro-thermal and damage phenomena in concrete at elevated temperatures. High temperature effects are considered by means of temperature and pressure dependence of several material parameters. Based on the mathematical model, the computer code HITECOSP was developed. Material parameters of the model were measured by several European laboratories, which participated in the "HITECO" research project. A model problem, concerning hygro-thermal behaviour and degradation of a HPC structure during fire, is solved. The influence of two different constitutive descriptions of the concrete permeability changes at high temperature, including thermo-chemical and mechanical damage effects, upon the results of computer simulations is analysed and discussed.

• Food Science of Animal Resources
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• v.35 no.2
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• pp.211-215
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• 2015

From the previous study, Enterococcus faecium SH01 was isolated from mukeunji, an over-ripened kimchi, and it produced bacteriocin SH01. Bacteriocin SH01 showed an inhibitory effect against Listeria monocytogenes ATCC 19111, a bacterial strain causing human listeriosis. Crude bacteriocin SH01 was purified by ammonium sulfate precipitation and its inhibitory activity at two concentrations (500 and 1,000 AU/g) against Listeria monocytogenes ATCC 19111 was investigated in ground beef at increasing temperatures (5, 10, 15, and 20℃) for 8 d. The number of Listeria monocytogenes ATCC 19111 significantly decreased (p<0.05) as the concentration of bacteriocin increased from 500 to 1,000 AU/g. Intrinsic crude protease activities in ground beef were examined and increased as the temperature increased. Experiments varying both the concentrations of added bacteriocin SH01 and temperature demonstrated a maximum inhibition (2.33 log reduction of bacteria) in samples containing 1,000 AU/g of bacteriocin SH01 incubated at 20℃. When the crude bacteriocin SH01 solution (1,280 AU/mL) was incubated with crude protease solutions at different temperatures, its activity decreased by only half (640 AU/mL), as assessed in an agar well diffusion assay. The finding that the antilisterial activity of bacteriocin SH01 increased with temperature can be explained by the fact that higher temperatures increase bacterial membrane fluidity, thereby promoting the cellular penetration of bacteriocin SH01 into L. monocytogenes. Bacteriocin SH01 may be an excellent candidate as a biopreservative for controlling L. monocytogenes growth in ground beef.

• Journal of Pharmacopuncture
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• v.19 no.4
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• pp.312-318
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• 2016

Objectives: Bacterial resistant infections have become a global health challenge and threaten the society's health. Thus, an urgent need exists to find ways to combat resistant pathogens. One promising approach to overcoming bacterial resistance is the use of herbal products. Green tea catechins, the major green tea polyphenols, show antimicrobial activity against resistant pathogens. The present study aimed to investigate the effect of catechins, green tea extract, and methylxanthines in combination with gentamicin against standard and clinical isolates of Staphylococcus aureus (S. aureus) and the standard strain of Pseudomonas aeruginosa (P. aeruginosa). Methods: The minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) values of different agents against bacterial strains were determined. The interactions of green tea extract, epigallate catechin, epigallocatechin gallate, two types of methylxanthine, caffeine, and theophylline with gentamicin were studied in vitro by using a checkerboard method and calculating the fraction inhibitory concentration index (FICI). Results: The MICs of gentamicin against bacterial strains were in the range of $0.312-320{\mu}g/mL$. The MIC values of both types of catechins were $62.5-250{\mu}g/mL$. Green tea extract showed insufficient antibacterial activity when used alone. Methylxanthines had no intrinsic inhibitory activity against any of the bacterial strains tested. When green tea extract and catechins were combined with gentamicin, the MIC values of gentamicin against the standard strains and a clinical isolate were reduced, and synergistic activities were observed (FICI < 1). A combination of caffeine with gentamicin did not alter the MIC values of gentamicin. Conclusion: The results of the present study revealed that green tea extract and catechins potentiated the antimicrobial action of gentamicin against some clinical isolates of S. aureus and standard P. aeruginosa strains. Therefore, combinations of gentamicin with these natural compounds might be a promising approach to combat microbial resistance.

• Journal of the Korean Society of Tobacco Science
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• v.13 no.1
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• pp.19-26
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• 1991

Ames test using special strains which are histidine requiring mutant of Salmonella typhimufium , is widely utilized as short-term bioassay to evaluate the genotoxicity of chemicals. This method requires the liver microsome(5-9 fraction) to provide mammalian metabolism of the compounds, Therefore, the mutagenic potency of the chemicals is affected by not only the intrinsic properties of them but also the efficiency of the in vitro microsomal activation system. For this reason, the complex mixtures such as environmental pollutants from occupational sources, natural products or cigarette smoke condensates(CSC) , might be often appeared the false results. Induction of sister chromatid exchange(SCE) in cultured cells is known as another sensitive and powerful tool for the measurement of genotoxicity and the method has also an advantage which is able to apply to the in vitro and in vivo systems. In the present study, the inducibilities of revertant colonies in tester strain TA98 and SCE in human Iymphocytes by positive controls and total particulated materials(TPM) obtained from various brand(domastic and imported) cigarettes were compared in order to investigate whether the results in Ames test are in agreement with those in SCE analysis. The mutagenic activities of well known mutagens such as B(a)P showed excellent dose-response in the both methods although the induction mechanism was different each other, but cyclophosphamide resulted such effect only in SCE analysis. Most TPM tested showed a similar pattern in the mutagenic activities in those methods. However, only two(one imported brand and one domestic sample cigarettes) among the TMP obtained from various cigarettes appeared the higher induction in SCE than Ames test.

• Journal of Korean Orthopaedic Sports Medicine
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• v.9 no.1
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• pp.16-21
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• 2010

The prevalence of overuse syndrome in the lower extremity including chronic compartment syndrome and stress fracture is increasing with popularity of sports activities. Chronic compartment syndrome is defined as elevation of the interstitial pressure during exertional activities in a closed osseofascial compartment that results in microvascular compromise and operative procedures can be necessary if conservative treatments fail. Stress fracture can be classified as fatigue and insufficiency fracture; stress fracture occurs by repeated strain under abnormal conditions from the patient's activity whereas insufficiency fracture does by those from a process intrinsic to the bone. Most stress fractures occur in the lower extremity, most commonly in the tibial region. Fatigue fractures begin in athletes with the change in their training programs. The radiographic findings are usually diagnostic or at least strongly suggestive and MRI has proven to be a beneficial diagnostic tool for difficult diagnostic cases. Fatigue fractures are treated with a decrease in activity, but surgical procedure may be necessary in those in anterior cortex of the tibial diaphysis.