Journal of Pharmacopuncture (대한약침학회지)

KOREAN PHARMACOPUNCTURE INSTITUTE (대한약침학회)
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Effect of Catechins, Green tea Extract and Methylxanthines in Combination with Gentamicin Against Staphylococcus aureus and Pseudomonas aeruginosa - Combination therapy against resistant bacteria -

  • Bazzaz, Bibi Sedigheh Fazly (Biotechnology Research Center, School of Pharmacy, Mashhad University of Medical Sciences) ;
  • Sarabandi, Sahar (Students' Research Committee, School of Pharmacy, Mashhad University of Medical Sciences) ;
  • Khameneh, Bahman (Department of Pharmaceutical Control, School of Pharmacy, Mashhad University of Medical Sciences) ;
  • Hosseinzadeh, Hossein (Pharmaceutical Research Center, Department of Pharmacodynamy and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences)
  • Received : 2016.06.25
  • Accepted : 2016.11.28
  • Published : 2016.12.31
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Abstract

Objectives: Bacterial resistant infections have become a global health challenge and threaten the society's health. Thus, an urgent need exists to find ways to combat resistant pathogens. One promising approach to overcoming bacterial resistance is the use of herbal products. Green tea catechins, the major green tea polyphenols, show antimicrobial activity against resistant pathogens. The present study aimed to investigate the effect of catechins, green tea extract, and methylxanthines in combination with gentamicin against standard and clinical isolates of Staphylococcus aureus (S. aureus) and the standard strain of Pseudomonas aeruginosa (P. aeruginosa). Methods: The minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) values of different agents against bacterial strains were determined. The interactions of green tea extract, epigallate catechin, epigallocatechin gallate, two types of methylxanthine, caffeine, and theophylline with gentamicin were studied in vitro by using a checkerboard method and calculating the fraction inhibitory concentration index (FICI). Results: The MICs of gentamicin against bacterial strains were in the range of $0.312-320{\mu}g/mL$. The MIC values of both types of catechins were $62.5-250{\mu}g/mL$. Green tea extract showed insufficient antibacterial activity when used alone. Methylxanthines had no intrinsic inhibitory activity against any of the bacterial strains tested. When green tea extract and catechins were combined with gentamicin, the MIC values of gentamicin against the standard strains and a clinical isolate were reduced, and synergistic activities were observed (FICI < 1). A combination of caffeine with gentamicin did not alter the MIC values of gentamicin. Conclusion: The results of the present study revealed that green tea extract and catechins potentiated the antimicrobial action of gentamicin against some clinical isolates of S. aureus and standard P. aeruginosa strains. Therefore, combinations of gentamicin with these natural compounds might be a promising approach to combat microbial resistance.

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References

  1. Khameneh B, Diab R, Ghazvini K, Fazly Bazzaz BS. Breakthroughs in bacterial resistance mechanisms and the potential ways to combat them. Microb Pathog. 2016;95:32-42. https://doi.org/10.1016/j.micpath.2016.02.009
  2. Khademi F, Poursina F, Hosseini E, Akbari M, Safaei HG. Helicobacter pylori in Iran: a systematic review on the antibiotic resistance. Iran J Basic Med Sci. 2015;18(1):2-7.
  3. Riley MA, Robinson SM, Roy CM, Dennis M, Liu V, Dorit RL. Resistance is futile: The bacteriocin model for ad-dressing the antibiotic resistance challenge. Biochem Soc Trans. 2012;40(6):1438-42. https://doi.org/10.1042/BST20120179
  4. Pantosti A, Sanchini A, Monaco M. Mechanisms of antibiotic resistance in Staphylococcus aureus. Future Microbiol. 2007;2(3):323-34. https://doi.org/10.2217/17460913.2.3.323
  5. Corvec S, Tafin F, Betrisey B, Borens O, Trampuz A. Activities of fosfomycin, tigecycline, colistin, and gen-tamicin against extended-spectrum-lactamase-producing Escherichia coli in a foreign-body infection mod-el. Antimicrob Agents Chemother. 2013;57(3):1421-7. https://doi.org/10.1128/AAC.01718-12
  6. Forouzanfar F, Bazzaz BSF, Hosseinzadeh H. Black cumin (Nigella sativa) and its constituent (thymoqui-none): a review on antimicrobial effects. Iran J Basic Med Sci. 2014;17(12):929-38.
  7. Khameneh B, Fazly Bazzaz BS, Amani A, Rostami J, Vahdati-Mashhadian N. Combination of anti-tuber-culosis drugs with vitamin C or NAC against different Staphylococcus aureus and mycobacterium tuberculosis strains. Microb Pathog. 2016;93:83-7. https://doi.org/10.1016/j.micpath.2015.11.006
  8. Hagihara M, Crandon JL, Nicolau DP. The efficacy and safety of antibiotic combination therapy for infections caused by gram-positive and gram-negative organisms. Expert Opin Drug Saf. 2012;11(2):221-33. https://doi.org/10.1517/14740338.2012.632631
  9. Olosunde OF, Abu-Saeed K, Abu-Saeed MB. Phytochemical screening and antimicrobial properties of a common brand of black tea (Camellia sinensis) marketed in Nigerian environment. Adv Pharm Bull. 2012;2(2):259-63. https://doi.org/10.5681/apb.2012.040
  10. Hamilton-Miller JM. Antimicrobial properties of tea (Camellia sinensis L.). Antimicrob Agents Chemother. 1995;39(11):2375-7. https://doi.org/10.1128/AAC.39.11.2375
  11. Yam TS, Shah S, Hamilton-Miller JM. Microbiological activity of whole and fractionated crude extracts of tea (Camellia sinensis), and of tea components. FEMS Microbiol Lett. 1997;152(1):169-74. https://doi.org/10.1111/j.1574-6968.1997.tb10424.x
  12. Chan EW, Soh EY, Tie PP, Law YP. Antioxidant and antibacterial properties of green, black, and herbal teas of Camellia sinensis. Pharmacognosy Res. 2011;3(4):266-72. https://doi.org/10.4103/0974-8490.89748
  13. Betts JW, Wareham DW. In vitro activity of curcumin in combination with epigallocatechin gallate (EGCG) versus multidrug-resistant Acinetobacter baumannii. BMC Microbiol. 2014;14:172. https://doi.org/10.1186/1471-2180-14-172
  14. Sharma A, Gupta S, Sarethy IP, Dang S, Gabrani R. Green tea extract: possible mechanism and antibacterial activity on skin pathogens. Food Chem. 2012;135(2):672-5. https://doi.org/10.1016/j.foodchem.2012.04.143
  15. Sourabh A, Kanwar SS, Sud RG, Ghabru A, Sharma OP. Influence of phenolic compounds of Kangra tea [Camellia sinensis (L) O Kuntze] on bacterial pathogens and indigenous bacterial probiotics of Western Himalayas. Braz J Microbiol. 2013;44(3):709-15. https://doi.org/10.1590/S1517-83822013000300007
  16. Osterburg A, Gardner J, Hyon SH, Neely A, Babcock G. Highly antibiotic-resistant acinetobacter baumannii clinical isolates are killed by the green tea polyphenol (-)-epigallocatechin-3-gallate (EGCG). Clin Microbiol Infect. 2009;15(4):341-6. https://doi.org/10.1111/j.1469-0691.2009.02710.x
  17. Zhao WH, Hu ZQ, Hara Y, Shimamura T. Inhibition of penicillinase by epigallocatechin gallate resulting in restoration of antibacterial activity of penicillin against penicillinase-producing Staphylococcus aureus. Anti-microb Agents Chemother. 2002;46(7):2266-8. https://doi.org/10.1128/AAC.46.7.2266-2268.2002
  18. Elgaher WA, Hayallah AM, Salem OIA, Abdel Alim AAM. Synthesis, anti-bronchoconstrictive, and antibacterial activities of some new 8-substituted-1,3-dimethylxan-thine derivatives. Bull Pharm Sci. 2009;32(1):153-87. https://doi.org/10.1248/bpb.32.153
  19. Hayallah AM, Elgaher WA, Salem OI, Alim AA. Design and synthesis of some new theophylline derivatives with bronchodilator and antibacterial activities. Arch Pharm Res. 2011;34(1):3-21. https://doi.org/10.1007/s12272-011-0101-8
  20. Bazzaz BS, Lavaei S, Hosseinzadeh H. Interaction of methylxanthines and gentamicin against Staphylococcus aureus and Pseudomonas aeruginosa: role of phos-phodiesterase inhibition. Acta Microbiol Immunol Hung. 2012;59(1):13-20. https://doi.org/10.1556/AMicr.59.2012.1.2
  21. Hosseinzadeh H, Bazzaz BSF, Sadati MM. In vitro evaluation of methylxanthines and some antibiotics: interaction against Staphylococcus aureus and Pseu-domonas aeruginosa. Iran Biomed J. 2006;10(3):163-7.
  22. Gyawali R, Adkins A, C. Minor R, Ibrahim SA. Behavior and changes in cell morphology of Escherichia coli O157:H7 in liquid medium and skim milk in the presence of caffeine. CYTA-J Food. 2014;12(3):235-41. https://doi.org/10.1080/19476337.2013.834977
  23. Kim YW, Chun HJ, Kim IW, Liu HB, Ahn WS. Antimicrobial and antifungal effects of green tea extracts against microorganisms causing vaginitis. Food Sci Biotechnol. 2013;22(3):713-9. https://doi.org/10.1007/s10068-013-0136-3
  24. Khameneh B, Iranshahy M, Ghandadi M, Ghoochi Atashbeyk D, Fazly Bazzaz BS, Iranshahi M. Investigation of the antibacterial activity and efflux pump inhibitory effect of co-loaded piperine and gentamicin nanoliposomes in methicillin-resistant Staphylococcus aureus. Drug Dev Ind Pharm. 2015;41(6):989-94. https://doi.org/10.3109/03639045.2014.920025
  25. Anita P, Sivasamy S, Madan Kumar PD, Balan IN, Ethi-raj S. In vitro antibacterial activity of Camellia sinensis extract against cariogenic microorganisms. J Basic Clin Pharm. 2014;6(1):35-9. https://doi.org/10.4103/0976-0105.145777
  26. Toda M, Okubo S, Ohnishi R, Shimamura T. [Antibacterial and bactericidal activities of Japanese green tea]. Nihon Saikingaku Zasshi. 1989;44(4):669-72. Japanese. https://doi.org/10.3412/jsb.44.669
  27. Lee JH, Shim JS, Chung MS, Lim ST, Kim KH. In vitro anti-adhesive activity of green tea extract against pathogen adhesion. Phytother Res. 2009;23(4):460-6. https://doi.org/10.1002/ptr.2609
  28. Chung JH, Han JH, Hwang EJ, Seo JY, Cho KH, Kim KH, et al. Dual mechanisms of green tea extract (EGCG)-induced cell survival in human epidermal keratinocytes. FASEB J. 2003;17(13):1913-5. https://doi.org/10.1096/fj.02-0914fje
  29. Taylor PW, Hamilton-Miller JM, Stapleton PD. Anti-microbial properties of green tea catechins. Food Sci Technol Bull. 2005;2:71-81.
  30. Rahal JJ Jr. Antibiotic combinations: the clinical relevance of synergy and antagonism. Medicine (Baltimore). 1978;57(2):179-95. https://doi.org/10.1097/00005792-197803000-00005

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