• The Korean Journal of Mycology
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• v.27 no.3 s.90
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• pp.191-197
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• 1999

The phylogenetic position of Gliocladium and its related taxa were investigated, using the neighbor-joining method of the sequences from internal transcribed spacers (ITS1 and ITS2) and 5.8S ribosomal DNA (rDNA). It was focused especially on the generic concept by comparing with the related genera such as Trichoderma, Hypocrea, Verticillium, Penicillium and Talaromyces. Gliocladium species and its related genus were divided into three groups by the phylogenetic analysis using the neighbor-joining method. The first group includes Penicillium-like strains such as Penicillium, Tararomyces, Verticillium and one species of Gliocladium (G. cibotii JCM 9203 and JCM 9206). Especially, Gliocladium cibotii JCM 9203 is thought to be the similar species with Verticillium bulbillosum JCM 9214. Between these two species, Gliocladium cibotii and Verticillium bulbillosum, the intraspecies concept needs to examined with culture condition. and morphological properties. The second group includes two species Verticillium, Verticillium tricorpus and Verticillium albo-atrum which extracted from the GenBank database in NCBI (National Center for Biotechnology Information). Trichoderma-like strains, such as Trichoderma, Hypocrea and several species of Gliocladium are included in the third group. Also, Gliocladium penicillioides IFO 5869 and Gliocladium catenulatum ATCC 10523 formed the subgroup of Trichoderma-like strains. The species of Gliocladium were dispersed in Trichoderma-like and Penicillinum-like group, and only one species of Gliocladium cihotii used in our study was located in Penicillium-like genus group. The species of Verticillium appeared in all three groups and the species of Trichoderma formed the monophylogeny with Hypocrea (telemorph). Also, Gliocladium virens was grouped with Trichoderma harzianum with a high bootstrap value, supporting that Gliocladium virens is to be placed in Trichoderma. The results suggest that Gliocladium is polyphyletic, and is more Trichoderma-like than Penicillium-like.

• Proceedings of the Korean Environmental Health Society Conference
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• 2005.12a
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• pp.95-102
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• 2005

For decolorization of synthetic dyes, Isolate fungi were investigated for the decolorization of 8 industrial dyes. One fungus isolated from textile wastewater collected from Banweol industrial complex, Korea showed excellent ability for removing synthetic dyes. Internal Transcribed Spacers (ITS) sequencing result was confirmed as the new Basidomycetes species. HUE05-1 The optimal decolorizaton conditions were pH5, 30$^{\circ}C$ and aerobic condition. HUE05-1 was completely decolorized all dyes in both solid and liquid condition. The result is decolorization effect at Reactive Orange 16; 97.12%, Reactive Blue 19; 92.09%, Reactive Blue 49; 97.04%, Reactive Yellow 145; 95.53%, Acid Orange 10; 99.18%, Acid Violet 43; 98.73%, Acid Blue 350; 94.71%, Disperse Blue 106; 90.07%.

• Mycobiology
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• v.43 no.2
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• pp.107-117
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• 2015

A mycoparasite, Scytalidium parasiticum sp. nov., isolated from the basidiomata of Ganoderma boninense causing basal stem rot of oil palm in Johor, Malaysia, is described and illustrated. It is distinct from other Scytalidium species in having smaller asci and ascospores (teleomorphic stage), longer arthroconidia (anamorphic stage), hyaline to yellowish chlamydospores, and producing a fluorescent pigment. The phylogenetic position of S. parasiticum was determined by sequence analyses of the internal transcribed spacers and the small-subunit ribosomal RNA gene regions. A key to identify Scytalidium species with teleomorphic stage is provided.

• The Korean Journal of Mycology
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• v.44 no.1
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• pp.64-67
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• 2016

During screening fungi as potential biological control agents for plant parasitic fungi, a fungal strain, EML-FAM3, was isolated from powdery mildew leaf lesions caused by Erysiphe platani on sycamore (Platanus occidentalis L.), and another strain, EML-FAMC1, from Erysiphe australiana on crape myrtle (Lagerstroemia indica L.). Based on the morphological characteristics and phylogenetic analysis of the internal transcribed spacers (ITS1 and ITS2) and 5.8S rDNA, the strains were identified as Ampelomyces quisqualis. To our knowledge, this is the first report of new mycohosts, E. platani and E. australiana, of the mycoparasite A. quisqualis on sycamore and crape myrtle plants. The hyperparasite may represent the potential for controlling E. platani and E. australiana epidemics.

• Journal of Environmental Health Sciences
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• v.32 no.4 s.91
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• pp.381-385
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• 2006

For decolorization of synthetic dyes, One fungus(HUE05-1) which was isolated from textile wastewater collected from industrial complex in Korea showed excellent ability of removing synthetic dyes. This fungus was identified as Basidiomycetes species by Internal Transcribed Spacers (ITS) sequence. Isolated fungi. HUE05-1 completely decolorized all dyes in both solid and liquid condition. The decolorization results were Reactive Orange-16, 97.12%; Reactive Blue-19, 92.09%; Reactive Blue-49, 97.04%; Reactive Yellow-145, 95.53%; Acid Orange-10, 99.18%; Acid Violet-43, 98.73%; Acid Blue-350, 94.71% and Disperse Blue-106, 90.07%.

• 한국약용작물학회:학술대회논문집
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• 2010.10a
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• pp.149-150
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• 2010

• Korean Journal Plant Pathology
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• v.14 no.2
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• pp.157-163
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• 1998

Genetic diversity among 27 isolates of Rhizoctonia solani, which were obtained from diseased crops in Korea and classified into 9 intraspecific groups by anastomosis test and cultural characteristics, was studied by PCR-RFLP. Gene regions of nuclear 17S ribosomal DNA and internal transcribed spacers including 5.8S rDNA of the isolates were amplified with polymerase chain reaction and digested with 12 restriction enzymes. Differences of restriction patterns were not shown among isolates within each intraspecific groups, however, each anastomosis group and culturala type sowed unique restriction fragment length polymorphisms by restriction patterns using HaeIII, Cfr13I and MspI. The results suggest that PCR-FRLP of rDNA using three restriction enzymes could be used to differentiate intraspecific groups of Rhizoctonia solani in Korea.

• Proceedings of the Korean Society of Fisheries Technology Conference
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• 2001.05a
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• pp.437-438
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• 2001

Species of the genus Pseudodiaptomus are reported as the major constituents in the fresh or brackish waters in the northeastern Asia (Suh et al., 1991; Oka et al., 1991). Of these species, P. inopinus (Burckhardt) and P. poplesia (Shen) are particularly numerous in spring and autumn (Suh et al., 1991; Shen and Song, 1979). Recently. we found a presence of two types of Pseudodiaptomus inopinus during the course of investigation on zooplankton from Korean estuaries. (omitted)

• Journal of Plant Biotechnology
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• v.38 no.4
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• pp.301-307
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• 2011

The medicinal herb Oldenlandia diffusa is known as a folk medicine for the treatment of hepatitis, sore throat, appendicitis, malignant tumors and urethral infection in Southern China and Korea. Another species O. corymbosa, is also used for the therapy of the similar conditions, however, only O. diffusa is referred to the medicinal herb by Chinese Pharmacopoeia. Due to their similar morphology, O. diffusa and O. corymbosa are often misidentified. To easily identify O. diffusa from O. corymbosa, the phylogenetic utility of nuclear ribosomal DNA (nrDNA) internal transcribed spacers (ITS) were investigated among different O. diffusa and O. corymbosa populations in Korea. The nrDNA ITS sequence of O. diffusa contained 791 bp, with GenBank accession number of JF837601-JF837602. The nrDNA ITS sequence of O. corymbosa was 785-786 bp, with GenBank accession number of JF837603-JF837611. The results showed that there are some certain divergences in the ITS region sequence between both species, even among different populations of the same species. Particularly, O. corymbosa ST-4 population showed the highest dissimilarity of the ITS region sequence with other nine populations of O. corymbosa and two populations of O. diffusa. This consequence makes us further understand the molecular diversification between O. corymbosa and O. diffusa, and help to promote the correct use and safety.

• Journal of Microbiology and Biotechnology
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• v.9 no.6
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• pp.744-750
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• 1999

Forty alcohol yeast strains were isolated from the main mashes (10 strains from each mash) for brewing of 4 different kinds of Korean traditional liquor (3 different types of Yakju and 1 Takju). Thirty-eight out of 40 strains were identified to be the same strain, Saccharomyces boulardii, by the Automated Bacteria, Yeast, and Fungi Identification System (Biolog Co., U.S.A.) based on the metabolic fingerprints. One strain that showed the highest ethanol production among the 38 strains in YPD medium, designated SHY 111, was selected and used for differentiating from other yeast type strains using the polymerase chain reaction (PCR). Amplified DNA, from transcribed internal spacers of SHY 111 chromosomal DNA, was found to be the same in both size and sequence as those of S. cerevisiae KCCM 11215 (formerly S. coreanus) and S. boulardii along with that of S. cerevisiae AB 972, which was used as a type strain for the yeast genome project. However, when PCR was carried out with the intron splice site primer, it resulted in the amplification of the SHY 111-specific DNA fragment which was about 200 bp in size. When PCR was carried out using the primer to test diversity of 40 isolated yeast strains, it was found that the PCR patterns were similar to each other except for the 200 bp bands derived from all the 10 strains from one Yakju, and 2 strains from another Yakju. These results suggest the strain identified as S. boulardii by the Automated Identification System to be a dominant strain for the fermentation of Korean traditional liquors.