The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
권호 표지

Phylogenetic Analysis of the Genus Gliocladium and its Related Taxa by Comparing the Sequences of Internal Transcribed Spacers and 5.8S r-DNA

Ribosomal DNA의 Internal Transcribed Spacer(ITS) 부위의 염기서열 분석에 의한 Gliocladium 속과 근연속에 관한 계통 분류학적 연구

  • Park, Ju-Young (Department of Microbiology, College of Natural Sciences, Pusan National University) ;
  • Kim, Gi-Young (Department of Microbiology, College of Natural Sciences, Pusan National University) ;
  • Ha, Myoung-Gyu (Korea Basic Science Institute) ;
  • Shin, Young-Kook (Korea Collection for Type Cultures, Korea Research Institute of Bioscience and Biotechnology) ;
  • Park, Yong-Ha (Korea Collection for Type Cultures, Korea Research Institute of Bioscience and Biotechnology) ;
  • Lee, Tae-Ho (Department of Microbiology, College of Natural Sciences, Pusan National University) ;
  • Lee, Jae-Dong (Department of Microbiology, College of Natural Sciences, Pusan National University)
  • 박주영 (부산대학교 자연과학대학 미생물학과) ;
  • 김기영 (부산대학교 자연과학대학 미생물학과) ;
  • 하명규 (기초과학지원연구소 부산분소) ;
  • 신용국 (생명공학연구소 유전자은행) ;
  • 박용하 (생명공학연구소 유전자은행) ;
  • 이태호 (부산대학교 자연과학대학 미생물학과) ;
  • 이재동 (부산대학교 자연과학대학 미생물학과)
  • Published : 1999.06.30
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Abstract

The phylogenetic position of Gliocladium and its related taxa were investigated, using the neighbor-joining method of the sequences from internal transcribed spacers (ITS1 and ITS2) and 5.8S ribosomal DNA (rDNA). It was focused especially on the generic concept by comparing with the related genera such as Trichoderma, Hypocrea, Verticillium, Penicillium and Talaromyces. Gliocladium species and its related genus were divided into three groups by the phylogenetic analysis using the neighbor-joining method. The first group includes Penicillium-like strains such as Penicillium, Tararomyces, Verticillium and one species of Gliocladium (G. cibotii JCM 9203 and JCM 9206). Especially, Gliocladium cibotii JCM 9203 is thought to be the similar species with Verticillium bulbillosum JCM 9214. Between these two species, Gliocladium cibotii and Verticillium bulbillosum, the intraspecies concept needs to examined with culture condition. and morphological properties. The second group includes two species Verticillium, Verticillium tricorpus and Verticillium albo-atrum which extracted from the GenBank database in NCBI (National Center for Biotechnology Information). Trichoderma-like strains, such as Trichoderma, Hypocrea and several species of Gliocladium are included in the third group. Also, Gliocladium penicillioides IFO 5869 and Gliocladium catenulatum ATCC 10523 formed the subgroup of Trichoderma-like strains. The species of Gliocladium were dispersed in Trichoderma-like and Penicillinum-like group, and only one species of Gliocladium cihotii used in our study was located in Penicillium-like genus group. The species of Verticillium appeared in all three groups and the species of Trichoderma formed the monophylogeny with Hypocrea (telemorph). Also, Gliocladium virens was grouped with Trichoderma harzianum with a high bootstrap value, supporting that Gliocladium virens is to be placed in Trichoderma. The results suggest that Gliocladium is polyphyletic, and is more Trichoderma-like than Penicillium-like.

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References

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