• Title/Summary/Keyword: Internal transcribed spacer rDNA

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The First Report of Postharvest Stem Rot of Kohlrabi Caused by Sclerotinia sclerotiorum in Korea

  • Kim, Joon-Young;Aktaruzzaman, Md.;Afroz, Tania;Hahm, Young-Il;Kim, Byung-Sup
    • Mycobiology
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    • v.42 no.4
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    • pp.409-411
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    • 2014
  • In March 2014, a kohlrabi stem rot sample was collected from the cold storage room of Daegwallyong Horticultural Cooperative, Korea. White and fuzzy mycelial growth was observed on the stem, symptomatic of stem rot disease. The pathogen was isolated from the infected stem and cultured on potato dextrose agar for further fungal morphological observation and to confirm its pathogenicity, according to Koch's postulates. Morphological data, pathogenicity test results, and rDNA sequences of internal transcribed spacer regions (ITS 1 and 4) showed that the postharvest stem rot of kohlrabi was caused by Sclerotinia sclerotiorum. This is the first report of postharvest stem rot of kohlrabi in Korea.

A New Record of Pseudallescheria boydii Isolated from Crop Field Soil in Korea

  • Babu, A. Giridhar;Kim, Sang Woo;Yadhav, Dil Raj;Adhikari, Mahesh;Kim, Changmu;Lee, Hyang Burm;Lee, Youn Su
    • Mycobiology
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    • v.42 no.4
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    • pp.397-400
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    • 2014
  • Pseudallescheria boydii KNU13-2 was isolated from crop field soil and identified by analysis of internal transcribed spacer regions of rDNA and morphological characteristics. In the literature, P. boydii has been mentioned as a human pathogen. This is the first record of P. boydii isolated from crop field soil in Korea.

First Report of Simplicillium cylindrosporum Isolated from Soil in Korea

  • Park, Sangkyu;Lee, Seung-Yeol;Lee, Jae-Jin;Lee, Hyang Burm;Ten, Leonid;Jung, Hee-Young
    • The Korean Journal of Mycology
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    • v.45 no.2
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    • pp.139-144
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    • 2017
  • The fungal strain KNU16-006 was isolated from field soil in Daegu, Korea. The isolate was identified as Simplicillium cylindrosporum on the basis of its morphological characterization and phylogenetic analysis using internal transcribed spacer regions of rDNA. This species has not been previously reported in Korea.

Phylogenetic Status of an Unrecorded Species of Curvularia, C. spicifera, Based on Current Classification System of Curvularia and Bipolaris Group Using Multi Loci

  • Jeon, Sun Jeong;Nguyen, Thi Thuong Thuong;Lee, Hyang Burm
    • Mycobiology
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    • v.43 no.3
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    • pp.210-217
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    • 2015
  • A seed-borne fungus, Curvularia sp. EML-KWD01, was isolated from an indigenous wheat seed by standard blotter method. This fungus was characterized based on the morphological characteristics and molecular phylogenetic analysis. Phylogenetic status of the fungus was determined using sequences of three loci: rDNA internal transcribed spacer, large ribosomal subunit, and glyceraldehyde 3-phosphate dehydrogenase gene. Multi loci sequencing analysis revealed that this fungus was Curvularia spicifera within Curvularia group 2 of family Pleosporaceae.

Diversity of Ectomycorrhizal fungi of Abies koreana at Mt. Halla (한라산 구상나무(Abies koreana)의 외생균근의 다양성)

  • Sim, Mi-Young;Eo, Ju-Kyeong;Eom, Ahn-Heum
    • The Korean Journal of Mycology
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    • v.37 no.2
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    • pp.134-138
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    • 2009
  • This study was conducted to investigate colonization of ectomycorrhizal fungi(ECM) in roots of Abies koreana which is an endemic and endangered species in Korea. Roots of A. koreana were collected at Mt. Halla. ECM root tips were classified using morphotyping and identified using sequences of internal transcribed spacer (ITS) region of the fungal rDNA. Total 8 species of ECM fungi were identified from roots of 11 seedlings of A. koreana : Cenococum geophilum, Russula brevipes, 2 species of Russula, 2 species of Thelephora, Cortinarius camphorates and 2 species of Helotiales. These species were known to be typical ectomycorrhizal fungi found in coniferous mature forests.

A New Record of Pseudeurotium bakeri from Crop Field Soil in Korea

  • Adhikari, Mahesh;Kim, Sangwoo;Yadav, Dil Raj;Um, Yong Hyun;Kim, Hyun Seung;Lee, Hyang Burm;Lee, Youn Su
    • The Korean Journal of Mycology
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    • v.44 no.3
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    • pp.145-149
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    • 2016
  • Pseudeurotium bakeri KNU14-21 isolated from crop field soil is a previously unreported fungal species in Korea. This fungal species was identified through morphological and molecular characteristics. Based on the shape and size of conidia and conidiophores as well as the internal transcribed spacer region of rDNA, the isolate was confirmed to be Pseudeurotium bakeri. This species has not been previously reported from Korea, and this is the first report of its incidence in Korea.

Neocosmospora rubicola, an Unrecorded Endophytic Fungus Isolated from Roots of Glycyrrhiza uralensis in Korea

  • Kim, Jin-Hee;Kim, Dong-Yeo;Park, Hyeok;Cho, Jae Hee;Eom, Ahn-Heum
    • The Korean Journal of Mycology
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    • v.45 no.1
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    • pp.63-67
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    • 2017
  • Through a survey of symbiotic endophytic fungi, we isolated an endophyte fungal strain from the roots of Glycyrrhiza uralensis in Korea. The isolated fungal strain was identified using its morphological characteristics and through phylogenetic analysis of the internal transcribed spacer, the large subunit rDNA region, and the translation elongation factor region. The strain was identified as Neocosmospora rubicola. This species has not been previously reported in Korea. In this study, we report its isolation from the roots of Glycyrrhiza uralensis in Korea, followed by the characterization and identification of the strain.

Characterization of Two New Records of Mucoralean Species Isolated from Gut of Soldier Fly Larva in Korea

  • Nguyen, Thi Thuong Thuong;Duong, Tham Thi;Lee, Hyang Burm
    • Mycobiology
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    • v.44 no.4
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    • pp.310-313
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    • 2016
  • While surveying the diversity of fungi of the order Mucorales, two isolates, EML-PUKI12-1 and EML-PUKI06-1, were obtained from the gut of soldier fly larvae inhabiting the bulrush at a pond located in the Chonnam National University Arboretum, Gwangju, Korea. The isolates were confirmed as Mucor irregularis and Mucor fragilis species, respectively, based on the morphological characteristics and phylogenetic analysis of rDNA internal transcribed spacer region. Such mucoralean species belonging to undiscovered taxa has not previously been described in Korea.

New Rust Disease of Korean Willow (Salix koreensis) Caused by Melampsora yezoensis, Unrecorded Pathogen in Korea

  • Yun, Yeo Hong;Ahn, Geum Ran;Yoon, Seong Kwon;Kim, Hoo Hyun;Son, Seung Yeol;Kim, Seong Hwan
    • Mycobiology
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    • v.44 no.4
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    • pp.335-337
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    • 2016
  • During the growing season of 2015, leaf specimens with yellow rust spots were collected from Salix koreensis Andersson, known as Korean willow, in riverine areas in Cheonan, Korea. The fungus on S. koreensis was identified as the rust species, Melampsora yezoensis, based on the morphology of urediniospores observed by light and scanning electron microscopy, and the molecular properties of the internal transcribed spacer rDNA region. Pathogenicity tests confirmed that the urediniospores are the causal agent of the rust symptoms on the leaves and young stems of S. koreensis. Here, we report a new rust disease of S. koreensis caused by the rust fungus, M. yezoensis, a previously unrecorded rust pathogen in Korea.

Characterization of Paecilomyces variotii and Talaromyces amestolkiae in Korea Based on the Morphological Characteristics and Multigene Phylogenetic Analyses

  • Nguyen, Thi Thuong Thuong;Paul, Narayan Chandra;Lee, Hyang Burm
    • Mycobiology
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    • v.44 no.4
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    • pp.248-259
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    • 2016
  • During fungal diversity surveys of the order Eurotiales in Korea, two fungal strains, EML-DG33-1 and EML-NCP50, were isolated from samples of rat dung and fig tree leaf collected at a garden located in Gwangju in 2014. To complete the National Species List of Korea, it is a prerequisite to verify whether many questionable species, which were previously recorded but not confirmed, indeed present in Korea. Herein, the isolates were confirmed as undescribed species, Paecilomyces variotii and Talaromyces amestolkiae based on the combination of morphological and phylogenetic analyses of multigenes including the rDNA internal transcribed spacer, ${\beta}-tubulin$, and RNA polymerase II subunit 2.