• 센서학회지
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• 제24권4호
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• pp.270-273
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• 2015

One of the main problems when working the chemical sensor is the lack of repeatability and reproducibility of the sensor response. If the problem is not properly taken into consideration, the stability and reliability of the system using chemical sensors would be decreased. In this paper we analyzed the sensor's drift of short term and proposed a compensation method for reducing the effects of the drift in order to improve the stability and the reliability of the chemical sensor. The sensor drift was analyzed by a trend line graph and CV(coefficient of variation) was used to quantify. And we compensated for the drift by using the internal normalization. As a result it was found that the value of CV was decreased after compensation.

• 센서학회지
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• 제25권4호
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• pp.291-296
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• 2016

The ideal chemical sensor must show the similar result under the same condition for accurate measurement of gases regardless of time. However, the actual responses of chemical sensors have been shown the lacks of repeatability and reproducibility because of the drift which has been caused by aging and pollution of the sensor and the environment change such as temperature and humidity. If the problems are not properly taken into considerations, the stability and reliability of the system using chemical sensors would be decreased. In this paper, we analyzed the sensor's drift and applied the three different compensation methods(DWT( Discrete Wavelets Transform), Baseline Manipulation, Internal Normalization) for reducing the effects of the drift in order to improve the stability and the reliability of short term of the chemical sensors. And in order to compare the results of the methods, the standard deviation was used as a criterion. The sensor drift was analyzed by a trend line graph. We applied the three methods to the successive data measured for three days and compared the results. As a result of comparison, the standard deviation of DWT showed lowest value. (Before compensation: 7.1219, DWT: 1.3644, Baseline Manipulation: 2.5209, Internal Normalization: 3.1425).

• 무역학회지
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• 제44권1호
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• pp.31-44
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• 2019

This study examines the normalization of Korean GM management between the Korean government and GM in terms of external negotiation game and internal negotiation game using Putnam's Two-Level Games. In addition, GM's Win-set change and negotiation strategy were analyzed. This analysis suggested implications for the optimal negotiation strategy for mutual cooperation between multinational corporations and local governments in the global business environment. First, the negotiation strategy for Korea's normalization of GM management in Korea can be shifted to both the concession theory and the opposition theory depending on the situation change and the government policy centered on the cautious theory. Second, GM will maximize its bargaining power through 'brink-end tactics' by utilizing the fact that the labor market is stabilized, which is the biggest weakness of the Korean government, while maintaining a typical Win-set reduction strategy. GM will be able to restructure at any time in terms of global management strategy, and if the financial support of the Korean government is provided, it will maintain the local factory but withdraw the local plant at the moment of stopping the support. In negotiations on the normalization of GM management in Korea, it is necessary to prepare a problem and countermeasures for various scenarios and to maintain a balance so that the policy does not deviate to any one side.

• Journal of Biosystems Engineering
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• 제35권6호
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• pp.450-457
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• 2010

Although cherry tomato is one of major vegetables consumed in fresh vegetable market, the quality grading method is mostly dependant on size measurement using drum shape sorting machines. Using Visible/Near-infrared spectroscopy, apparatus to be able to acquire transmittance spectrum data was made and used to estimate firmness, sugar content, and acidity of cherry tomatoes grown at hydroponic and soil culture. Partial least square (PLS) models were performed to predict firmness, sugar content, and acidity for the acquired transmittance spectra. To enhance accuracy of the PLS models, several preprocessing methods were carried out, such as normalization, multiplicative scatter correction (MSC), standard normal variate (SNV), and derivatives, etc. The coefficient of determination ($R^2_p$) and standard error of prediction (SEP) for the prediction of firmness, sugar, and acidity of cherry tomatoes from green to red ripening stages were 0.859 and 1.899 kgf, with a preprocessing of normalization, 0.790 and $0.434^{\circ}Brix$ with a preprocessing of the 1st derivative of Savitzky Golay, and 0.518 and 0.229% with a preprocessing normalization, respectively.

• Asian-Australasian Journal of Animal Sciences
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• 제33권12호
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• pp.2021-2030
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• 2020

Objective: Quantitative polymerase chain reaction (qPCR) has been extensively used in the field of mesenchymal stem cell (MSC) research to elucidate their characteristics and clinical potential by normalization of target genes against reference genes (RGs), which are believed to be stably expressed irrespective of various experimental conditions. However, the expression of RGs is also variable depending on the experimental conditions, which may lead to false or contradictory conclusions upon normalization. Due to the current lack of information for a clear list of stable RGs in bovine MSCs, we conducted this study to identify suitable RGs in bovine MSCs. Methods: The cycle threshold values of ten traditionally used RGs (18S ribosomal RNA [18S], beta-2-microglobulin [B2M], H2A histone family, member Z [H2A], peptidylprolyl isomerase A [PPIA], ribosomal protein 4 [RPL4], succinate dehydrogenase complex, subunit A [SDHA], beta actin [ACTB], glyceraldehyde-3-phosphate dehydrogenase [GAPDH], TATA box binding protein [TBP], and hypoxanthine phosphoribosyltrasnfrase1 [HPRT1]) in bovine bone marrow-derived MSCs (bBMMSCs) were validated for their stabilities using three types of RG evaluation algorithms (geNorm, Normfinder, and Bestkeeper). The effect of validated RGs was then verified by normalization of lineage-specific genes (fatty acid binding protein 4 [FABP4] and osteonectin [ON]) expressions during differentiations of bBMMSCs or POU class 5 homeobox 1 (OCT4) expression between bBMMSCs and dermal skins. Results: Based on the results obtained for the three most stable RGs from geNorm (TBP, RPL4, and H2A), Normfinder (TBP, RPL4, and SDHA), and Bestkeeper (TBP, RPL4, and SDHA), it was comprehensively determined that TBP and RPL4 were the most stable RGs in bBMMSCs. However, traditional RGs were suggested to be the least stable (18S) or moderately stable (GAPDH and ACTB) in bBMMSCs. Normalization of FABP4 or ON against TBP, RPL4, and 18S presented significant differences during differentiation of bBMMSCs. However, although significantly low expression of OCT4 was detected in dermal skins compared to that in bBMMSCs when TBP and RPL4 were used in normalization, normalization against 18S exhibited no significance. Conclusion: This study proposes that TBP and RPL4 were suitable as stable RGs for qPCR study in bovine MSCs.

• 산업식품공학
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• 제15권4호
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• pp.324-331
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• 2011

초분광영상을 이용하여 방울토마토의 전체 면에서 반사스펙트럼을 획득하였으며 숙도 등급(GN-RD)에 따른 스펙트럼의 차이를 관찰하였다. 방울토마토의 반사스펙트럼에서 클로로필에 의한 675 nm 영역의 흡수가 관찰되었고, 당과 수분의 영향으로 알려진 840 nm, 970 nm 영역에서 흡수가 관찰되었다. 특히 GN에서 RD 등급으로 숙도가 진행될수록 평균 스펙트럼의 경우 반사율이 낮아지는 경향이 관찰되었다. 총 8개의 전처리를 이용하여 전 숙도 등급의 시료에 적용한 PLS 회귀 분석에서 내부품질들 중 경도 예측모델이 가장 우수한 것으로 확인되었다. 이때 전처리는 평균값을 이용한 정규화이었으며 결정계수는 0.876, 그리고 SEP은 1.875kgf이었다. 당도의 경우는 최대값을 이용한 정규화에서 결정계수가 0.823과 SEP $0.388^{\circ}Bx$로 나타났으며, 산함량의 경우 최대값을 이용한 정규화에서 0.620의 결정계수와 0.208%의 SEP이 확인되었다. 상품성을 고려한 PK, LR, RD 등급의 시료에서 PLS 회귀 분석을 실시한 결과 내부품질 중 전체의 숙도 등급의 시료를 사용하여 예측한 결과보다는 전체적으로 다소 낮은 예측결과를 확인할 수 있었다. 내부 품질 중 경도에서 가장 높은 예측모델이 확인되었으며, 전처리는 일정 범위를 이용한 정규화이고 0.679의 결정계수와 $0.976^{\circ}Bx$의 SEP이 확인되었다. 당도는 최대값을 이용한 정규화에서 0.586의 결정계수와 0.546kgf의 SEP의 결과를 보였으며 산 함량은 Savitzky Golay의 2차 미분에서 0.547의 결정계수와 0.188%의 SEP을 보였다. 본 연구에서는 최근 연구 활용이 시작되고 있는 최신기술인 초분광 반사영상을 이용하여 방울토마토 내부품질인 경도, 당도, 산 함량 예측의 가능성을 확인하였다. 초분광 영상은 영상처리를 이용하여 외부의 결함 및 외부 착색도등도 측정할 수 있으므로 본 연구에서 수행한 내부품질 측정과 융합하여 복합적인 농산물 품질 선별기 개발에 활용할 수 있을 것으로 판단된다.

• 대한한방내과학회지
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• 제42권4호
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• pp.707-717
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• 2021

Objectives: The aim of this study was to introduce the normalization effects of stimulation of both ST36 and CV11, 12, 13 meridian points on the abnormal gastric myoelectrical activity in two cases of functional dyspeptic patients with gastric dysmotility. Methods: Gastric myoelectrical activity was recorded by electrogastrography every two weeks until their gastric myoelectrical activity finally reached the normality. Dominant frequency of gastric slow waves in the fasting and postprandial periods and a dominant power ratio were obtained on each occasion. Patients were treated three times each day: 9 am, 1 pm, and 7 pm. The first treatment consisted of manual and immersion stimulation on all used meridian points for 20 min. In the second and third treatments, electrical stimulation of both ST36 was added. It was conducted for 20 min at a strength intensity of 1.2 times the pain threshold at a frequency of 3 Hz. Results: Stimulation of the above meridian points normalized abnormal gastric myoelectrical activity. The time taken to return from abnormal gastric myoelectrical activity to normal was 16-19 weeks. Conclusion: Stimulation of both ST36 and CV11, 12, 13 meridian points normalized the abnormal gastric myoelectrical activity in the functional dyspepsia of gastric dysmotility.

• 한국경영과학회지
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• 제39권3호
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• pp.117-131
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• 2014

As interests in the management of government-owned corporation rise, in these days, debt reduction and normalization of management of those companies have been proceeding under the lead of government. At this point, it is very important to seek internal marketing method for improvement of internal employees' satisfaction, loyalty and organization performance. This study analyzes impact of factors of internal marketing effect on organization satisfaction, loyalty and organization performance in the context of the domestic public companies, the Korea Electric Power Corporation (KEPCO) and the Korea Water Resources Corporation (K-WATER)'s employees empirically. There are some significant differences between the two publics. It is proved that delegation of the authority influences on internal satisfaction and organization performance in the case of KEPCO, and education and training influence on internal satisfaction and organization performance in the case of K-WATER. On the other hand, in the case of K-WATER, any internal marketing factors don't influence on loyalty. The results of this study show somewhat different characteristics depending on the characteristics of firm, however, it is expected that this study can be very useful in regards to similar public enterprises or businesses.

• 한국작물학회지
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• 제55권4호
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• pp.371-378
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• 2010

For sensitive and accurate gene expression analysis, normalization of gene expression data against housekeeping genes is required. There are conventional housekeeping gene (e.g. ACT) that primarily function as an internal control of transcription. In this study, we performed an in silico analysis of 278 rice gene expression samples (GSM) in order to identify the gene that is most consistently expressed. Based on this analysis, we identified novel candidate housekeeping genes that displayed improved stability among the cross experimental conditions. Furthermore four of the most conventional housekeeping genes were included in our 30 other housekeeping genes among the most stable genes. Therefore, these 30 genes can he used to normalize transcription results in gene expression studies on rice at a broad range of experimental conditions.

• 농업과학연구
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• 제39권3호
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• pp.413-420
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• 2012

Spectroscopic measurement method based on visible and near-infrared wavelengths was prominent technology for rapid and non-destructive evaluation of internal quality of fruits. Reflectance measurement was performed to evaluate firmness, soluble solid content, and acid content of truss tomatoes by hyperspectral reflectance imaging system. The Vis/NIR reflectance spectra was acquired from truss tomatoes sorted by 6 ripening stages. The multivariable analysis based on partial least square (PLS) was used to develop regression models with several preporcessing methods, such as smoothing, normalization, multiplicative scatter correction (MSC), and standard normal variate (SNV). The best model was selected in terms of coefficient of determination of calibration ($R_c^2$) and full cross validation ($R_{cv}^2$), and root mean standard error of calibration (RMSEC) and full cross validation (RMSECV). The results of selected models were 0.8976 ($R_p^2$), 6.0207 kgf (RMSEP) with gaussian filter of smoothing, 0.8379 ($R_p^2$), $0.2674^{\circ}Bx$ (RMSEP) with the mean of normalization, and 0.7779 ($R_p^2$), 0.1033% (RMSEP) with median filter of smoothing for firmness, soluble solid content (SSC), and acid content, respectively. Results show that Vis / NIR hyperspectral reflectance imaging technique has good potential for the measurement of internal quality of truss tomato.