• Title/Summary/Keyword: Internal membrane

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Production of Bacteriorhodopsin by Halobacterium halobium in the Internal Membrane Bioreactor (내부 필터 발효기에서 Halobacterium halobium의 배양에 의한 박테리오로돕신의 생산)

  • 엄영순;박준택;홍순호;이상엽;장호남
    • KSBB Journal
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    • v.13 no.3
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    • pp.268-271
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    • 1998
  • Bacteriorhodopsin in the purple membrane (PM) of halobacteria has recently been attracting much attention to be used as a component of molecular electron device and optical computers. In order to increase the productivity of bacteriorhodopsin in high cell density cultures of Halobacterium halobium R1, an internal membrane cell-retention bioreactor system was employed. As a result, the production of cell mass at OD660 of 12 and of bacteriorhodopsin at 125-130 mg/L were obtained using the internal membrane bioreactor system at a dilution rate of 0.066 hr-1. The productivity achieved by the internal membrane system (0.7 mg/L$.$hr) was 3.5-fold higher than that obtained by the corresponding batch cultivations (0.2 mg/L$.$hr).

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Characterization of the Putative Membrane Fusion Peptides in the Envelope Proteins of Human Hepatitis B Virus

  • Kang, Ha-Tan;Yu, Yeon-Gyu
    • Bulletin of the Korean Chemical Society
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    • v.28 no.10
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    • pp.1756-1762
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    • 2007
  • Envelope proteins of virus contain a segment of hydrophobic amino acids, called as fusion peptide, which triggers membrane fusion by insertion into membrane and perturbation of lipid bilayer structure. Potential fusion peptide sequences have been identified in the middle of L or M proteins or at the N-terminus of S protein in the envelope of human hepatitis B virus (HBV). Two 16-mer peptides representing the N-terminal fusion peptide of the S protein and the internal fusion peptide in L protein were synthesized, and their membrane disrupting activities were characterized. The internal fusion peptide in L protein showed higher activity of liposome leakage and hemolysis of human red blood cells than the N-terminal fusion peptide of S protein. Also, the membrane disrupting activity of the extracellular domain of L protein significantly increased when the internal fusion peptide region was exposed to N-terminus by the treatment of V8 protease. These results indicate that the internal fusion peptide region of L protein could activate membrane fusion when it is exposed by proteolysis.

Dye removal from water using emulsion liquid membrane: Effect of alkane solvents on efficiency

  • Ghaemi, Negin;Darabi, Farzaneh;Falsafi, Monireh
    • Membrane and Water Treatment
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    • v.10 no.5
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    • pp.361-372
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    • 2019
  • Effect of different alkane based solvents on the stability of emulsion liquid membrane was investigated using normal alkanes (n-hexane, n-heptane, n-octane and n-decane) under various operating parameters of surfactant concentration, emulsification time, internal phase concentration, volume ratio of internal phase to organic phase, volume ratio of emulsion phase to external phase and stirring speed. Results of stability revealed that emulsion liquid membrane containing n-octane as solvent and span-80 (5 % (w/w)) as emulsifying agent presented the highest amount of emulsion stability (the lowest breakage) compared with other solvents; however, operating parameters (surfactant concentration (5% (w/w)), emulsification time (6 min), internal phase concentration (0.05 M), volume ratio of internal phase to organic phase (1/1), volume ratio of emulsion phase to external phase (1/5) and stirring speed (300 rpm)) were also influential on improving the stability (about 0.2% breakage) and on achieving the most stable emulsion. The membrane with the highest stability was employed to extract acridine orange with various concentrations (10, 20 and 40 ppm) from water. The emulsion liquid membrane prepared with n-octane as the best solvent almost removed 99.5% of acridine orange from water. Also, the prepared liquid membrane eliminated completely (100%) other cationic dyes (methylene blue, methyl violet and crystal violet) from water demonstrating the efficacy of prepared emulsion liquid membrane in treatment of dye polluted waters.

MATHEMATICAL MODELLING FOR THE AXIALLY MOVING MEMBRANE WITH INTERNAL TIME DELAY

  • Kim, Daewook
    • East Asian mathematical journal
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    • v.37 no.1
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    • pp.141-147
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    • 2021
  • In [1], we studied the PDE system with time-varing delay. Time delay occurs due to loosening in a high-speed moving axially directed membrane (string, belt, or plate) at production. Our purpose in this work derives a mathematical model with internal time delay. First, we consider the physical phenomenon of axially moving membrane with respect to kinetic energy, potential energy and work done. By the energy conservation law in physics, we get the second order nonlinear PDE system with internal time delay.

Characteristic of the Permeation Flux of Hollow Fiber Membranes by Process Pressures Change (공정압 변화에 따른 중공사막의 투과플럭스 특성)

  • Lee, Yong-Taek;Kim, Nam-Su;Shin, Dong-Ho
    • Membrane Journal
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    • v.17 no.4
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    • pp.318-328
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    • 2007
  • This study was carried out to evaluate the performance of the separate membrane (HF; hollow fiber membrane with polysulfone) process applied with the external membrane types, internal pressure membrane types and external-internal types according to the variations of pressure and membrane pore size in the purification treatment process of the lake water. The maximum permeate flux was average values of 282 LMH and 234 LMH with the pore size of 0.3 and 0.05 ${\mu}m$ respectively in the external pressure membrane process, and 443 LMH and 522 LMH with the pore size of 0.3 and $0.05{\mu}m$ respectively in the internal pressure membrane process. In addition, the maximum permeate flux of the process that was applied with external and internal membrane pressure simultaneously showed the average values of 674 LMH with the pore size of $0.3{\mu}m$, and 648 LMH with the pore size of $0.05{\mu}m$. Therefore, maximum yield per unit area is supposed when the separate membrane that was applied with external and internal pressure simultaneously are used to treat the lake water.

Physical Properties of Covered Stent in Gastric Acid Environment: In Vitro Study (위산 환경에서 피막형 스텐트의 물성 변화)

  • Park, Sung Chul;Park, Nark-Soon;Kim, Dong-Gon;Nah, Jae-Woon;Jeen, Yoon Tae;Cho, Hye Jin;Kim, Eun Sun;Keum, Bora;Seo, Yeon Seok;Lee, Hong Sik;Chun, Hoon Jai;Um, Soon Ho;Kim, Chang Duck;Ryu, Ho Sang
    • Polymer(Korea)
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    • v.38 no.3
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    • pp.351-357
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    • 2014
  • In membrane covered stent, occlusion and fracture from membrane degradation by gastric acid sometimes occurred. Therefore, we investigated the physical properties of membrane covered stent according to its ingredient and concentration in gastric acid environment. Membrane covered stents consisted of silicone and polyurethane with 15%, 18%, 20% concentrations were used. After incubating stents in a condition of pH 1.2, we checked any changes at every 3 weeks for 18 weeks. The changes of membrane surface, radial expansion and recovery force of stent were investigated. Coating thickness increased proportionally to an increase in ingredient concentration. Surface was evenly coated with silicone compared to the case with polyurethane and its homogeneity was excellent in a high concentration. Degradation was much severe in the case of polyurethane. The radial force of silicone was higher than polyurethane, and the decrease of radial and recovery force was higher in the case of polyurethane. In conclusion, high concentration of silicone membrane was more stable than polyurethane in acid environment of in vitro study.

Removal study of As (V), Pb (II), and Cd (II) metal ions from aqueous solution by emulsion liquid membrane

  • Dohare, Rajeev K.;Agarwal, Vishal;Choudhary, Naresh K.;Imdad, Sameer;Singh, Kailash;Agarwal, Madhu
    • Membrane and Water Treatment
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    • v.13 no.4
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    • pp.201-208
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    • 2022
  • Emulsion Liquid Membrane (ELM) is a prominent technique for the separation of heavy metal ions from wastewater due to the fast extraction and is a single-stage operation of stripping-extraction. The selection of the components (Surfactant and Carrier) of ELM is a very significant step for its preparation. In the ELM technique, the primary water- in-oil (W/O) emulsion is emulsified in water to produce water-in-oil-in-water (W/O/W) emulsion. The water in oil emulsion was prepared by mixing the membrane phase and internal phase. To prepare the membrane phase, the extractant D2EHPA (di-2-ethylhexylphosphoric acid) was used as a mobile carrier, Span-80 as a surfactant, and Paraffin as a diluent. Moreover, the internal (receiving) phase was prepared by dissolving sulphuric acid in water. Di-(2- ethylhexyl) phosphoric acid such as surfactant concentration, carrier concentration, sulphuric acid concentration in the receiving (internal) phase, agitation time (emulsion phase and feed phase), the volume ratio of the membrane phase to the receiving phase, the volume ratio of the external feed phase to the primary water-in-oil emulsion and pH of feed were studied on the percentage extraction of metal ions at 20℃. The results show that it is possible to remove 78% for As(V), 98% for Cd(II), and 99% for Pb(II). Emulsion Liquid Membrane (ELM) is a well-known technique for separating heavy metal ions from wastewater due to the fast extraction and is a single-stage operation of stripping-extraction. The selection of ELM components (Surfactant and Carrier) is a very significant step in its preparation. In the ELM technique, the primary water-in-oil (W/O) emulsion is emulsified to produce water-in-oil-in-water (W/O/W) emulsion. The water in the oil emulsion was prepared by mixing the membrane and internal phases. The extractant D2EHPA (di-2-ethylhexylphosphoric acid) was used as a mobile carrier, Span-80 as a surfactant, and Paraffin as a diluent. Moreover, the internal (receiving) phase was prepared by dissolving sulphuric acid in water. Di-(2-ethylhexyl) phosphoric acid such as surfactant concentration, carrier concentration, sulphuric acid concentration in the receiving (internal) phase, agitation time (emulsion phase and feed phase), the volume ratio of the membrane phase to the receiving phase, the volume ratio of the external feed phase to the primary water-in-oil emulsion and pH of feed were studied on the percentage extraction of metal ions at 20℃. The results show that it is possible to remove 78% for As(V), 98% for Cd(II), and 99% for Pb(II).

Application of high voltage pulse for reduction of membrane fouling in membrane bio-reactor and kinetic approach to fouling rate reduction (막결합형 생물반응기(Membrane Bio-Reactor)의 막 오염 저감을 위한 고전압 펄스의 적용과 막 오염 저감 속도론적 해석)

  • Kim, Kyeong-Rae;Kim, Wan-Kyu;Chang, In-Soung
    • Journal of Korean Society of Water and Wastewater
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    • v.34 no.3
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    • pp.183-190
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    • 2020
  • Although membrane bio-reactor (MBR) has been widely applied for wastewater treatment plants, the membrane fouling problems are still considered as an obstacle to overcome. Thus, many studies and commercial developments on mitigating membrane fouling in MBR have been carried out. Recently, high voltage impulse (HVI) has gained attention for a possible alternative technique for desalting, non-thermal sterilization, bromate-free disinfection and mitigation of membrane fouling. In this study, it was verified if the HVI could be used for mitigation of membrane fouling, particularly the internal pore fouling in MBR. The HVI was applied to the fouled membrane under different conditions of electric fields (E) and contact time (t) of HVI in order to investigate how much of internal pore fouling was reduced. The internal pore fouling resistance (Rf) after HVI induction was reduced as both E and t increased. For example, Rf decreased by 19% when the applied E was 5 kV/cm and t was 80 min. However, the Rf decreased by 71% as the E increased to 15 kV/cm under the same contact time. The correlation between E and t that needed for 20% of Rf reduction was modeled based on kinetics. The model equation, E1.54t = 1.2 × 103 was obtained by the membrane filtration data that were obtained with and without HVI induction. The equation states the products of En and t is always constant, which means that the required contact time can be reduced in accordance with the increase of E.

Removal of safranin from aqueous solution through liquid emulsion membrane

  • Lohiya, Roshni;Goyal, Arihant;Dohare, Rajeev Kumar;Agarwal, Madhu;Upadhyaya, Sushant
    • Membrane and Water Treatment
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    • v.10 no.5
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    • pp.373-379
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    • 2019
  • One of the real issues of the recent years is water contamination because of harmful synthetic dyes. Liquid Membranes (LM) resemble a promising alternative to the current separation processes, demonstrating various points of interest as far as effectiveness, selectivity, and operational expenses. The improvement of various Liquid Membranes designs has been a matter of examination by few researchers, particularly for the expulsion of dyes from aqueous solutions. The choice of organic surfactants plays an essential role in the efficiency of the dye removal. In LM design, the most significant step towards productivity is the decision of the surfactant type and its concentration. Liquid emulsion membrane (LEM) was used to remove safranin from aqueous solutions in which the emulsion was made with the help of D2EHPA as carrier, kerosene was used as a diluent and Span 80 (Sorbiton monooleate) was used as an emulsifying agent or surfactant. Various sorts of internal stages were utilized, to be specific sulphuric acid and sodium hydroxide. The impact of parameters influencing extraction efficiency such as pH of feed solution, concentrations of surfactant and emulsifying agent in membrane phase, volume ratio of internal phase to membrane phase, internal phase concentration, agitation speed and time of extraction were analyzed.

Characteristics of Fouling in a Submerged Membrane Bioreactor Activated Sludge Process (침지형 막분리 활성 슬러지법에 따른 막 오염 특성)

  • 김대식;강종석;김기연;이영무
    • Membrane Journal
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    • v.11 no.4
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    • pp.170-178
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    • 2001
  • PVC microfiltration membrane was prepared by phase immersion method and applied to membrane bioreactor (MBR) contained activated sludge. The hydrophilicity of membrane and the pore size increase with the amount of additive(PVP) ducting the preparation of membrane. Permeation characteristics and the membrane fouling behavior were investigated by varying the internal environment in MBR using the prepared membranes. When there is a sludge bulking in MBR caused by microorganism, membrane fouling was accumulated. The cake layer resistance, R$_{c}$, of membrane increased in the order of CP-0 > CP-1.0 > CP-1.5. Rc increased up to 3.5~7 fold where the sludge bulking occurred in MBR. CP-1.5 seems to be appropriated membrane on the basis of the surface characteristics and the flux. The average flux of all the test membrane was 12(${\pm}$2) L/$m^2$hr whereas the COD removal efficiency was 98.8%. The ratio of bulking sludge and the type and the size of microorganism in operating MBR accelerate the membrane fouling and flux decline. It is concluded that the characteristic of membrane filtration depends on the hydrophilicity of membrane, the internal environment of MBR reactor and the growth factor of sludge.

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