• Title/Summary/Keyword: Internal Transcribed Spacer

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New Record of Anthracnose Caused by Colletotrichum liriopes on Broadleaf Liriope in Korea

  • Oo, May Moe;Oh, Sang-Keun
    • The Korean Journal of Mycology
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    • v.45 no.1
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    • pp.68-73
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    • 2017
  • In 2015, the leaves of broadleaf liriope, Liriope muscari, showing anthracnose symptoms were found in a field of Korea. The fungus was isolated and identified as Colletotrichum liriopes based on the morphological characteristics and nucleotide sequence data of the glyceraldehyde-3-phosphate dehydrogenase, internal transcribed spacer and actin genes. To the best of our knowledge, this is the first report of C. liriopes isolated from L. muscari in Korea.

Genetic Relationship of Cordyceps spp. Based on Internal Transcribed Spacer Sequences of Ribosomal DNA (Ribosomal DNA의 ITS 염기서열에 의한 동충하초속균의 유연관계)

  • 남성희;황재삼;조세연;구태원
    • Journal of Sericultural and Entomological Science
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    • v.41 no.3
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    • pp.174-179
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    • 1999
  • The genetic relationships among six Cordyceps spp. were investigated based on internal transcribed spacer sequences of ribosomal DNA .A portion of the these genes was amplified by PCR. Approximately 590 base pairs were successfully amplified, cloned, sequenced, compared. The nucleotide sequence of the six amplified fragments were aligned by the clustal W program. As a result, Cordyceps militaris shared 87, 96, 98, 90 and 97% sequences homology with Paecilomyces japonica, Paecilomyces sp. J300, Paeciomyces farinosa. Paecilomyces sp. J500 and Cordyceps sinensis, respectively. Paecilomyces japonica also shared 87, 88, 92 and 87% sequence similarity with Paecilomyces sp. J300, Paecilomyces farinosa, Paecilomyces sp. J500 and Cordyceps sinensis, repectively.

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Grovesinia moricola occurring on Parthenocissus tricuspidata

  • Shin, Hyeon-Dong;Choi, Young-Joon;Hong, Sun-Hee;Lee, Yong-Ho
    • The Korean Journal of Mycology
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    • v.47 no.3
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    • pp.271-274
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    • 2019
  • Between August and September 2017, zonate leaf spots with severe defoliation were observed on Parthenocissus tricuspidata in Gimcheon, Korea. The initial symptoms included water-soaked, gray to grayish brown, circular or irregular-shaped leaf spots. As the disease progressed, the spots enlarged, coalesced, and became cream-colored with characteristic target-shaped rings, leading to leaf blight and premature defoliation. Based on cultural and morphological characteristics, the fungus associated with the symptoms was identified as Grovesinia moricola. The identity of this fungus was confirmed by analyzing sequences of the internal transcribed spacer region of ribosomal DNA. The pathogenicity of the isolate was demonstrated by artificial inoculation. To our knowledge, this is the first report of G. moricola occurring on P. tricuspidata globally as well as in Korea.

Three Unreported Endophytic Fungi Isolated from Conifer Leaves of Pinus densiflora in Korea (소나무 침엽에서 분리된 3종의 국내 미기록 내생균)

  • Park, Hyeok;Eom, Ahn-Heum
    • The Korean Journal of Mycology
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    • v.47 no.1
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    • pp.35-42
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    • 2019
  • We isolated endophytic fungi from the conifer leaves of Pinus densiflora inhabiting Buan-gun, Jeollabuk-do, Korea. We identified the isolated fungal strains based on phylogenetic analysis performed using the nucleotide sequences of the internal transcribed spacer, large subunit, and beta-tubulin. We confirmed the presence of three novel endophytic fungi in Korea, namely Paracamarosporium hawaiiense, Tubakia dryina, and Zasmidium fructigenum. In this report, we described the morphological characteristics of these fungal strains and the results of their phylogenetic analysis.

Erysiphe cornicola, a Powdery Mildew Occurring on Cornus controversa in Korea

  • In-Young Choi;Lamiya Abasova;Joon-Ho Choi;Ji-Hyun Park;Hyeon-Dong Shin
    • The Korean Journal of Mycology
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    • v.51 no.1
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    • pp.57-62
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    • 2023
  • In Korea, Cornus controversa and C. florida are known as hosts of Erysiphe pulchra from section Microspharea of the genus Erysiphe. However, recent molecular-phylogenetic analyses on the internal transcribed spacer regions and large subunit gene of the rDNA revealed that the Erysiphe powdery mildew on C. controversa in Japan is in fact E. cornicola. To assess the taxonomic status of Erysiphe-C. controversa association in Korea, isolates collected since 1987 were investigated and consequently identified as E. cornicola based on molecular-phylogenetic analyses and new morphological traits. To our knowledge, this is the first study to confirm the presence of this powdery mildew in Korea.

Report on the Unrecorded Helvella Genus Found in Taebaek City, South Korea

  • Sangyoung Park;Sohee Kim;Eunjin Kim;Ju-Kyung Eo;Hwayong Lee
    • The Korean Journal of Mycology
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    • v.51 no.4
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    • pp.411-417
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    • 2023
  • In July 2023, previously unrecorded fungi belonging to the genus Helvella were collected from the city of Taebaek, South Korea. These fungi are morphologically similar to Helvella crispa, but their differences include a wide, saddle-shaped apothecium and white hair on the receptacle surface. By analyzing DNA sequences combining the internal transcribed spacer (ITS) and nuclear ribosomal large subunit (nrLSU) regions and comparing them with various related species of Helvella, the collected fungi were identified as H. orienticrispa.

Three new records of mushroom species from Hangyeong-Andeok Gotjawal, South Korea

  • Seung-Hak Lee;Dae-Ju Oh;Weon-Jong Yoon;Yong-Hwan Jung
    • The Korean Journal of Mycology
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    • v.51 no.3
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    • pp.191-204
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    • 2023
  • This study introduces one specimen each of the genera Gymnopilus, Marasmius, and Mycena as an unrecorded species found in the Hwasun Gotjawal and Jeju Gotjawal Provincial Park of the Hangyeong-Andeok terrain, South Korea. Morphological analyses of macroscopic and microscopic features, and molecular analysis of the nuclear ribosomal internal transcribed spacer region were conducted to identify the specimens. Our results indicated that the newly identified specimens were Gymnopilus dilepis, Marasmius tenuissimus, and Mycena seminau. Here, we describe these newly identified mushroom species and provide baseline data on their diversity in Gotjawal, South Korea.