• Tuberculosis and Respiratory Diseases
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• v.77 no.5
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• pp.203-208
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• 2014

Background: In this study, we investigated whether lobetyolin, lobetyol, and methyl linoleate derived from Codonopsis pilosula affect MUC5AC mucin secretion, production, and gene expression from airway epithelial cells. Methods: Confluent NCI-H292 cells were pretreated with lobetyolin, lobetyol, or methyl linoleate for 30 minutes and then stimulated with phorbol 12-myristate 13-acetate (PMA) for 24 hours. The MUC5AC mucin gene expression, and mucin protein production and secretion were measured by reverse transcription polymerase chain reaction and enzyme-linked immunosorbent assay, respectively. Results: Lobetyolin, lobetyol, and methyl linoleate inhibited the gene expression of MUC5AC mucin induced by PMA; lobetyolin did not affect PMA-induced MUC5AC mucin production. However, lobetyol and methyl linoleate inhibited the production of MUC5AC mucin; lobetyolin and lobetyol did not significantly affect PMA-induced MUC5AC mucin secretion from NCI-H292 cells. However, methyl linoleate decreased the MUC5AC mucin secretion. Conclusion: These results suggest that among the three compounds, methyl linoleate can regulate gene expression, production, and secretion of MUC5AC mucin by directly acting on the airway epithelial cells.

• Journal of the Korean Geotechnical Society
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• v.20 no.8
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• pp.181-191
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• 2004

For a rigid retaining wall with rough face, the magnitude and distribution of active earth pressure on the wall are affected by the shape of failure surface and arching effect developed in the backfill as well as internal friction angle of the backfill and wall friction angle. Therefore, the practical shape of failure surface and arching effect in the backfill must be considered to acquire accurate magnitude and non-linear distribution of active earth pressure acting on the rigid retaining wall. In this study, a new formulation for calculating the active earth pressure on a rough rigid retaining wall rotating about the top is proposed considering the practical shape of non-linear failure surface and arching effects. Accuracy of the proposed equation is checked through comparisons of calculations from the proposed equations with existing model test results. The comparisons show that the proposed equations produce satisfactory results.

• Journal of Korean Tunnelling and Underground Space Association
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• v.9 no.2
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• pp.183-204
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• 2007

When a tunnel is excavated below groundwater table, the groundwater flows into the excavated wall of tunnel and seepage forces are acting on the tunnel wall. Such seepage forces significantly affect the ground reaction curve which is defined as the relationship between internal pressure and radial displacement of tunnel wall. In this paper, seepage forces arising from the ground water flow into a tunnel were estimated quantitatively. Magnitude of seepage forces was decided based on hydraulic gradient distribution around tunnel. Using these results, the theoretical solutions of ground reaction curve with consideration of seepage forces under steady-state flow were derived. A no-support condition and a supported condition with grouted bolts and shotcrete lining were considered, respectively. The theoretical solution derived in this study was validated by numerical analysis. The changes in the ground reaction curve according to various cover depths and groundwater table conditions were investigated. Based on the results, the application limit of theoretical solutions was suggested.

• Preventive Nutrition and Food Science
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• v.5 no.4
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• pp.184-188
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• 2000

HMG-CoA reductase is th rate-limiting enzyme of cholesterol biosynthesis. As intracellular levels of cholesterol should be regulated elaborately in response to external stimuli an internal needs, the expression of the HMG-CoA reductase gene is regulated intricately at several different levels from transcription to post-translational modification. In this study, we investigated the regulatory mechanism of HMG-CoA reductase gene expression at the post-transcriptional/pre-translational levels in a baby hamster kidney cell line, C100. when 25-hydroxycholesterol was added to cells cultured in medium containing 5% delipidized fetal bovine serum and 25$\mu$M lovastatin, the levels of HMG-CoA reductase mRNA decreased rapidly, which seemed to be due to the increased degradation of reductase mRNA. These suppressive effects of 25-hydroxycholesterol on MG-CoA reductase mRNA levels were blocked by a translation inhibitor, cycloheximide. Similarly, actinomycin D and 5,6-dichloro-1-$\beta$-D-ribofuranosylbenzimidazole, transcription inhibitors, blocked the 25-hydroxycholesterol-mediated degradation of HMG-CoA reductase mRNA. These results indicate that new protein/RNA synthesis is required for the degradation of HMG-CoA reductase mRNA. In addition, data from the transfection experiments shows that cis-acting determinants, regulating the stability of reductase mRNA, were scattered in the sequence corresponding to 1766-4313 based on the sequence of Syrian hamster HMG-CoA reductase cDNA. Our data suggests that sterol-mediated destabilization of reductase mRNA might be one of the important regulatory mechanism of HMG-CoA reductase gene expression.

• Journal of the Korean Society of Costume
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• v.59 no.7
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• pp.86-99
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• 2009

The audience of the performing arts enacted on the stage appreciate acting of the characters in a play to understand its plot, identify with the characters, and comprehend the message and subject matters of the play. Stage make-up in particular should assist audience in comprehending the story without overpowering or distracting the script. Stage make-up is a crucial means to visualize occupation, class, personality, and age of the characters. Thus it is an essential means in producing a high quality performance. The aim of the study is to examine character-emphasis make-up for a play by reviewing theories on the concepts and methods of the stage make-up. The methodology of the study was to analyze character make-up of the adopted play The Madwoman of Chaillot (La Folle de Chaillot) translated from the original French, as a case study. The technique and features of the character make-up of the play were examined, and functions of each factor of the character make-up depending on a role were also examined. The results of the study are as follows. The stage make-up for creating and expressing a distinctive personality of the characters in a play must integrate environmental factors external to a performance such as lighting and scale of a venue. Moreover the stage make-up must adopt and reconstruct internal factors of the play such as interpretation of the original work, subject matters, interpretation of the script, character analysis based on the discussion with a director, and design setting.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.40 no.8
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• pp.711-717
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• 2012

During the conceptual design phase of a light sport aircraft, the wind tunnel tests were conducted to investigate the static stability of newly-designed configuration. The 1/5 scale-down wind tunnel model consisted of fuselage, main wing, vertical tail and horizontal tail. The main wing and tails were able to be attached or detached from the fuselage. The aerodynamic forces and moments acting on the 6 different configurations compounding each component were measured by using the internal balance system and their static stability derivatives were derived. With these experimental data, the baseline lift and drag characteristics as well as the effects of each component to the longitudinal, directional and lateral static stability were quantitatively analyzed.

• Journal of the Korea institute for structural maintenance and inspection
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• v.18 no.1
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• pp.31-39
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• 2014

In order to design rigid zone area of frame structures, it is important to predict the direction change of internal stresses according to the bending moment acting on the joint region. In this study, it was examined whether current highway bridge design standards are useful to design different types of rigid joints having a various haunch shapes. In addition, stress distributions of inside of various rigid joints were inspected using the linear elastic finite element analysis. Based on the results of finite element analysis, the strut-tie models to design rigid joints are proposed. Suggested by this study, the strut-tie models have a same level of accuracy to a linear elastic finite element analysis. The proposed strut-tie models will be useful to design reinforcement details of rigid joints having a various haunch types.

• Journal of Sasang Constitutional Medicine
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• v.16 no.3
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• pp.1-7
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• 2004

1. Objectives This research is purposed to present developmental direction of traditional Korean medicine, through summarizing original medical mind of traditional Korean medicine. 2. Methods It was researched as bibliographic study with chief writings of traditional Korean medicine such as "DongEuiBoGam(東醫寶鑑)" and "Dongyi Suse Bowon(東醫壽世保元)" 3. Results and Conclusions (1) Huh Jun regarded Three Precious(三要: 精, 氣, 神) as important factors in Morpho-imaginary viewpoints. So he suggested finding out the pathological states according to the states of Three Precious. (2) As Huh Jun recognized that the essence(精) is the origin of the body, he suggested the medical mind of laying emphasis on the essence. And he suggested that circulating of the essence is important to health and longevity, which is different from previous trational medicne laying emphasis on the tonifying of five organs(五臟). (3) Dong-mu suggested diagnostic method that is to find out "Natural Principle of Organ(臟腑性理)" by observation of "Knowing-Acting(知行)" and "Morphological aspect and Temper(體形氣像)". And he suggested finding out pathological states according to states of "Good Qi of each constitution(體質正氣)". (4) Dong-mu recognized that the disease should be treated by recovering "Good Qi of each constitution" with both pharmacotherapy and non-pharmacotherapy. So he uggested the medical mind of laying emphasis on "Good Qi of each constitution", which is different from previous trational medicne laying emphasis on the tonifying of five organs. (5) To be brief, Huh Jun layed emphasis on the essence and Dong-mu layed emphasis on "Good Qi of each constitution". Emphasizing internal vitality which is applyed to treatment and prevention of disease is the original medical mind of traditional Korean medicine.

• Tuberculosis and Respiratory Diseases
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• v.48 no.6
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• pp.879-886
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• 2000

Background : Thyroid Transcription Factor-1(TTF-1) acts as a tissue specific transcription factor in the regulation of lung specific gene expression and as morphogenic protein during lung organogenesis. Currently, there is very little information on the cis-acting sequences and transcription factors that direct the TTF-1 gene expression. DNAse 1 hypersensitive (DH) sites represent a marker for active or potentially active chromatin and are likely to be especially important in gene regulation, being associated with many DNA sequences that regulate gene expression. It is clear that DH regions correlate with genetic regulatory loci and binding for sequence-specific DNA-binding proteins. Methods : We have used DH site assays to identify putative distal regulatory elements in H441 lung adenocarcinoma cells, which express the TTF-1 gene and HeLa cells. Results : There are four DH sites 5' of the TTF-1 gene. These sites are located at base pair approximately +150, -450, -800, and -1500 from the start of transcription. Conclusion : These data suggest that there may be at least one intragenic site and regulatory region 5' prime to the promotor region.

• Proceedings of the Korean Society of Propulsion Engineers Conference
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• 2010.11a
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• pp.235-240
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• 2010

Transpiration cooling is the most effective cooling technique for liquid rocket and air-breathing engines operating in aggressive environments with higher pressures and temperatures. Combustor liners and turbine vanes are cooled by the coolant(air or fuel) passing through their porous walls and also the exit coolant acting as an insulating film. However, its practical implementation has been hampered by the limitations of available porous materials. The search for more practical methods of increasing the internal heat transfer within the walls has led to the development of multi-laminate porous structures, such as Lamilloy$^{(R)}$ and Transply$^{(R)}$. This paper reviews recent research activities of transpiration cooling for the propulsions of liquid rocket, gas turbine, and scramjet.