• Title/Summary/Keyword: Intermediate culture

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Artificial Seed Production Using the Reproduction Methods in Codium fragile (Chlorophyta) (녹조류 청각의 생식방법을 이용한 인공종묘생산)

  • Hwang, Eun-Kyoung;Baek, Jae-Min;Park, Chan-Sun
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.38 no.3
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    • pp.164-171
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    • 2005
  • Codium fragile (Suringar) Hariot, an edible green alga is farmed in Korea by natural blooming zygotes attachment. Experiments were conducted to reveal the conditions for artificial seed production of C. fragile by sexual and asexual reproduction. Growth was compared between zygotes attachment (sexual reproduction) and isolated utricles with medullary filaments (asexual reproduction). Zygotes and isolated utricles with medullary filaments were cultured under different light conditions (10, 20, 40, 60 and $100\;{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$) and temperatures (5, 10, 15, 20 and $25^{\circ}C$) under 16:8LD. Maximum growth of zygote was $261.3{\pm}21.0\;{\mu}m$ under $15^{\circ}C$ and $20\;{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ after 13 days culture. Maximum regeneration of isolated medullary filament was $8.1{\pm}1.7\;mm$ per one isolated utricle under $20^{\circ}C$ and $100\;{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ after 15 days culture. After intermediate culture during two months in the field, morphogenesis occurred in both sexual and asexual reproduction, and growth of young thalli was not significantly different (p>0.05) between the both reproduction methods. Even though seed production of C. fragile is possible in both sexual and asexual reproduction, the mass artificial seed production of asexual reproduction is much more effective than that of sexual reproduction that is too much affected by maturity.

A Study on the Factors Affecting Technological Innovation of Innovative IT SMEs (혁신형 IT 중소기업의 기술혁신에 영향을 미치는 요인에 관한 연구)

  • Kim, J.K.;Hwang, K.T.
    • Journal of Digital Convergence
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    • v.14 no.11
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    • pp.201-224
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    • 2016
  • The main objective of this study is to identify and analyze the factors affecting technology innovation of the innovative IT SMEs. A research model is established based on the previous studies on the technology innovation of SMEs. The model consists of independent variables (Adhocracy culture, R&D capability, IT capability, Leadership, and Appraisal & Reward), an intermediate variable (Knowledge management), and dependent variables (product/service innovation and process innovation). Major research results can be summarized as follows: IT SMEs pursuing technology innovation should: (1) pay attention to innovation oriented culture, IT capability, and evaluation & compensation systems of the company; (2) recognize that knowledge management is an essential activity performed by the company; (3) strengthen the R&D capabilities of the company by not only exerting internal efforts but establishing cooperative network with various parties; and (4) resolve the decoupling problem of the evaluation & compensation system.

A Study on Establishment of Rural Living Service Functions by Settlement Class (정주계층별 농촌생활서비스 기능정립 및 취약지역 기준에 관한 연구)

  • Cho, Young-Jae;Yun, Jeong-Mi;Han, Seung-seok;Jo, Seoung-Hyun
    • Journal of Korean Society of Rural Planning
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    • v.29 no.4
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    • pp.13-26
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    • 2023
  • Recently, South Korea has prepared laws and systems to systematically manage rural spaces in response to the era of population decline and is making various efforts to promote related policies. However, various basic studies that can support this are still insufficient. In particular, in this study, the functions and roles of each settlement class were established along with the classification of the sedentary classes in rural areas, and the classification system for rural living services was established, and the hierarchy by functional facilities and the minimum standards for vulnerable areas (accessibility) were established. Specifically, in this study, the settlement class was divided into 4 classes of "central area - midpoint area - small point area - hinterland", and each function and role was presented, and the rural living service classification system was finally reestablished as 10 sectors and 31 functional facilities. In addition, the hierarchy and accessibility standards of rural living service functional facilities was set within 5 to 15 minutes for 'lower and basic services', within 10 to 20 minutes for 'medium and basic services', within 15 to 30 minutes for 'intermediate and complex services', within 20 to 60 minutes for 'high car/complex service' and within 10 minutes for 'urgent service'.

Presumptive Diagnosis of Mycoplasma pneumoniae Pneumonia in Children (소아에서 마이코플라즈마 폐렴의 예기적 진단)

  • Lee, Chang Eon;Park, Su Jin;Kim, Won Duck
    • Journal of Yeungnam Medical Science
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    • v.29 no.2
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    • pp.89-95
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    • 2012
  • Background: As Mycoplasma pneumoniae pneumonia has increased in Korea, its relevance to infants, toddlers, and adolescents has magnified as well as. However, it is difficult to perform the serological test and PCR test routinely for diagnosis in actual clinical practice. Thus, the authors conducted this study to help clinicians do presumptive diagnosis of Mycoplasma pneumoniae pneumonia using clinical, radiological, and hematological findings. Methods: The study population consisted of 224 children between 1 month and 14 years old, hospitalized for radiographically confirmed pneumonia. Patients were divided into two groups of 100 children with Mycoplasma pneumoniae pneumonia, as diagnosed using the ELISA method. Groups with negative result in Mycoplasma IgM antibody test were classified into the viral group (98 patients with respiratory virus) and the bacterial group (46 patients with the bacteria detected in the blood sputum culture or antibiotic treatment except macrolide improved the patient's condition). These groups were compared and analyzed using clinical, hematological, and radiographic differences and scoring system. Results: Clinical, hematological, and radiographic characteristics of Mycoplasma pneumoniae pneumonia have shown the intermediate level results between bacterial pneumonia and viral pneumonia. In terms of scoring system, the mean score of Mycoplasma pneumoniae pneumonia was 4.23, which was the intermediate level between bacterial pneumonia (mean score=6.67) and viral pneumonia (mean score=1.48). Conclusion: Results suggest that the combination of the scoring system information can increase the accuracy in the diagnosis even if they may have difficulties on diagnosis, because clinical manifestations, hematological, and radiographic findings are nonspecific.

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An Evaluation for Regional Identity of Urban Parks and It's Utility -focused on the new towns in the Capital Region- (도시공원의 지역적 정체성 평가와 유용성 - 수도권 신도시를 중심으로 -)

  • 오정학;고동완;김유일
    • Journal of the Korean Institute of Landscape Architecture
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    • v.27 no.1
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    • pp.79-89
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    • 1999
  • Recent expansion of urban parks has been partly successful in terms of providing urban outdoor recreation space. Unfortunately we paid very little attention to the importance of providing regional identity to urban parks until recently. Thus one of the most important tasks of the contemporary landscape architects of Korea is to investigate ways to improve regional identity of urban parks. This study is aimed at evaluating how well the current urban parks have realized their own regional identity. To test the effectiveness of the concept of 'identity', the identity items were created, while a research model was developed by using such variables as in urban park identity, park satisfaction, belongingness to the region, and residential satisfaction. In order to test the model, the causations were analyzed. Meanwhile, the scales to evaluating identity were reviewed by means of the unstructures group interview, and were finally determined as 10 items; culture, uniqueness, symbolic, historic nature, homogeneity, harmony, difference, public benefit, nativeness and traditionality. Data from three new towns in the capital region-Bundang, Ilsan and Sanbon-were collected, and their central parks were referred to a questionnaire survey. The data were processed using descriptive statistics, correlation analysis, multiple regression analysis and path analysis. It was found through this survey that subjects 'sentiments regarding the sample towns' regional identity differed slightly. Residents of Bundang felt the strongest amount of regional identity, followed by those of Ilsan and Sanbon. In addition, the most important scale on regional identity is 'park satisfaction', followed by 'belongingness to the region' and 'residental satisfaction'. The path analysis was conducted to interpret the causations in a more detailed and comprehensive way than correlation analysis. As a result, it was proven that the regional identity affects 'belingingness to region' through the intermediated variable 'park satisfaction', while affecting ' residential satisfaction' through the intermediate variables 'park satisfaction' and 'beligningness to region'. In other words, although the regional identity of a park does not directly affect 'belongingness to region' or 'residental satisfaction', it has indirect effects on them through intermediate variables.

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Quality Strategy for Building a Smart Factory in the Fourth Industrial Revolution (4차 산업혁명시대의 스마트 팩토리 구축을 위한 품질전략)

  • Chong, Hye Ran;Bae, Kyoung Han;Lee, Min Koo;Kwon, Hyuck Moo;Hong, Sung Hoon
    • Journal of Korean Society for Quality Management
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    • v.48 no.1
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    • pp.87-105
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    • 2020
  • Purpose: This paper aims to propose a practical strategy for smart factories and a step-by-step quality strategy according to the maturity of smart factory construction. Methods: The characteristics, compositional requirements, and diagnosis system are examined for smart factories through theoretical considerations. Several cases of implementing smart factory are studied considering the company maturity level from the aspect of the smartness concept. And specific quality techniques and innovation activities are carefully reviewed. Results: The maturity level of smart factory was classified into five phases: 1) ICT non-application, 2) basic, 3) intermediate 1, 4) intermediate 2, 5) advanced level. A five-step quality strategy was established on the basis of case studies; identify, measure, analyze, optimize, and customize. Some quality techniques are introduced for step-by-step implementation of quality strategies. Conclusion: To build a successful smart factory, it is necessary to establish a quality strategy that suits the culture and size of the company. The quality management strategy proposed in this paper is expected to contribute to the establishment of appropriate strategies for the size and purpose of the company.

Culture Conditions of Garlic Resistant Lactic Acid Bacteria for Feed Additives (사료첨가용 생균제 개발을 위한 마늘 내성 유산균의 배양 조건)

  • Kim, Yu-Jin;Jang, Seo-Jung;Park, Jung-Min;Kim, Chang-Uk;Park, Young-Seo
    • Food Engineering Progress
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    • v.14 no.1
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    • pp.65-74
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    • 2010
  • Culture conditions of L. plantarum TJ-LP-002, the garlic resistant strain isolated from pakimchi (green onion kimchi), were investigated for the use of feed additives. Acetic acid, citric acid, lactic acid, and tartaric acid were detected in the culture supernatant, and especially the concentrations of lactic acid and acetic acid significantly increased during cultivation. The antimicrobial activity of L. plantarum TJ-LP-002 was not affected by proteases, calatase or cellulase, which showed that the antimicrobial activity might be due to the production of acids rather than proteinaceous antimicrobial substances. L. plantarum TJ-LP-002 was resistant to neomycin sulfate, spectinomycin dihydrochloride, and lincomycin hydrochloride, sensitive to streptomycin sulfate, and intermediate resistant to ampicillin trihydrate, chloramphenicol, erythromycin, tetracycline hydrochloride, and kanamycin sulfate. The optimum initial pH of medium, fermentation temperature and time for the cell growth and antibacterial activity were pH 7.0, 30${^{\circ}C}$ and 24hr, respectively. The optimal composition of culture medium for the cell growth and antimicrobial activity was 3%(w/v) glucose as a carbon source, 3%(w/v) yeast extract as a nitrogen source, and manganese sulfate and ammonium citrate as inorganic salts. The combinatorial supplementation of these inorganic salts, rather than sole addition as an inorganic salt, resulted in better antibacterial activity.

Comparative Evaluation of First Urine and Intermediate Urine Samples Collected using a Patented Urine Cup (특허 받은 소변컵을 이용하여 채집한 첫 소변과 중간소변 시료의 비교 평가)

  • Kim, SeungChul;Kim, HoSung;Kim, ChangUook;Pyo, SangShin
    • The Journal of the Korea Contents Association
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    • v.22 no.6
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    • pp.619-628
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    • 2022
  • In general, as a method to confirm a urinary tract infection (UTI) in a medical institutions, urine culture including a urinalysis and an antimicrobial susceptibility test is performed. It is important to disinfect the area around the urethra and perineum before collecting urine samples, and it is important to collect it intermediate urine, not the first-void urine. We invented a patent urine cup (Patent No. 10-1732843) that can automatically and easily separate first-void urine and midstream urine and using this, the patent cup and the general cup were compared and evaluated using this. Nitrite (P<0.001), WBC (P=0.005), Bacterial colony count (P=0.001), colony positivity rate (P=0.004) in first-void urine (N=24), midstream urine (N=24) separated by patent cup to obtain a significantly higher value. This can be seen from the fact that the first-void urine and midstream urine separated using the patent cup were well separated. Also, the number of Bacterial colonies was statistically significantly higher in the midstream urine isolated using a patent cup (N=24) than in the midstream urine collected using a general cup (N=24) (average 7.9 vs. 4.0 on average, P= 0.002). Which means that the midstream urine separated using the patent cup is more sensitive to the UTI test than the midstream urine collected using a general cup.

Optimal Pricing and Ordering Policies for an Exponential Deteriorating Product under Order-size-dependent Delay in Payments (주문량에 따라 종속적인 신용거래 하에 퇴화성제품의 최적 가격 및 재고정책)

  • Seong-Whan Shinn
    • The Journal of the Convergence on Culture Technology
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    • v.9 no.5
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    • pp.493-499
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    • 2023
  • Trade credit refers to a transaction where a product supplier allows an distributor to defer payment for a certain period of time for the purchase cost of the products. This practice is generally permitted as a means of differentiation between competing companies. Such trade credit is commonly granted based on the volume of transactions, aiming to increase customer orders. From the perspective of the distributor, trade credit allows for a deferred payment period for the purchase cost, leading to cost savings in inventory investment. These cost savings in inventory investment can be a factor in reducing selling prices with the aim of increasing customer demand. In this study, we analyze a model that determines the optimal selling price and order quantity from the perspective of the distributor, assuming that the supplier allows a deferred payment period dependent on the transaction volume. We assume that the final customer's annual demand exhibits an exponential decrease with respect to the distributor's selling price, using a constant price elasticity function. To analyze the problem, we assume that the product deteriorates at a constant rate over time and aim to establish an inventory model for the intermediate distributor. We also want to analyze the impact of deterioration on the inventory policies of the intermediate distributor.

Improved Early Survival in Backcrosses of Male Mud Loach (Misgurnus mizolepis)$\times$Cyprinid Loach (M. anguillicaudatus) Hybrids to Femal Cyprinid Loach (미꾸리, Misgurnus anguillicaudatus의 생존율 향상을 위한 역교배체 생산)

  • 박인석;김봉석;임재현;박효민;남윤권;정창화;김동수
    • Journal of Aquaculture
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    • v.10 no.3
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    • pp.363-371
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    • 1997
  • Backcross hybridization between female cyprinid loach(Misgurnus anguillicaudatus) any male mud loach (M. mizolepis)$\times$cyjprinid loach hybrid were made, and its effect on early survival, cytogenetic traits, and gonad development were examined. Mean fertilization rate and hatching success of backcross hybrids were similar to those found in the maternal species, the cyprinid loach. However, the backcross hybrids revealed significantly improved early survival up to yolk sac absorption (86.0%) compared to cyprinid loach (0%) under low culture temperature ($15^{\circ}C$). the erythrocytic size and DNA content of backcross hybrids were nearly intermediate to those of the parents. Karyological analysis of backcross hybrids displayed two kinds of modal chromosome number of 2n=48 or 2n=49. Growth of backcross hybrids over 6 months after hatching was proven to be intermediate between their parental species. Although backcross hybrids had intermediate values between those of thier parents in most morphometric traits, overall external morphology of backcross hybrids was morer simlar to cyprinid loach than mud loach$\times$cyprinid loach hybrid. Histological examination of gonads at 2 and 4 months of age revealed that no female was obseved in backcross hybrid groups, suggesting the possibility of production of monosex male population.

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