• 한국잡초학회지
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• 제30권4호
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• pp.412-420
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• 2010

잡초는 농업생태계에서 경합에 의하여 막대한 피해를 입히며, 환경 및 인류의 부정적인 관심 때문에 잡초를 방제하기 위하여 사용되고 있는 합성 제초제에 대한 의존도를 줄이려는 노력이 전 세계적으로 이루어지고 있다. 이와 같은 관점에서 수수 추출물의 일종인 sorgoleone의 제초활성과 작물 선택성을 온실과 포장조건에서 검정하였다. Sorgoleone은 토양처리와 경엽처리에서 모두 높은 효과를 나타냈는데 화본과 잡초보다 광엽잡초에서 효과가 높았다. 온실조건에서 대부분 광엽잡초의 생장은 Sorgoleone $200\;{\mu}g\;ml^{-1}$에서 80%이상 억제되었고, 소리쟁이와 갈퀴덩굴은 완전히 고사되었다. 포장조건에서도 sorgoleone의 제초활성은 광엽잡초에 가장 우수하였고, 방동사니, 화본과 잡초 순이었다. 포장조건에서 잡초생장 억제정도는 전반적으로 온실조건보다 다소 낮은 경향이었다. 벼, 보리, 밀, 옥수수, 콩, 들깨, 토마토와 배추에서는 sorgoleone에 의한 생장억제는 거의 없었으나 상추와 오이는 생장이 억제되었다.

• 대한전자공학회논문지SD
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• 제37권1호
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• pp.44-51
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• 2000

Series Coupler Access Encodler(SCAE)와 Series Coupler Access Decoder(SCAD)를 이용한 광CDMA(Code Division Multiple Access)시스템에서 광정합필터 특성을 삼차신호까지 고려하여 분석하였다. 기존의 연구에서는 SCAE 및 ACAD를 평가할 때, 일차신호만을 고려하여 시스템의 성능을 분석하여 성능평가가 정확하지가 않았다. SCAE 및 SCAD는 커플러 수를 증가함에 따라 여러 형태의 간섭신호들을 가지므로, 이러한 신호들이 자기상관과 상호상관 세기를 변화시킨다. 그러므로 좀 더 정확한 시스템 성능을 분석하기 위해서는 간섭신호들의 특성을 연구할 필요성이 있다. 본 논문에서는 복호화 되는 광신호를 삼차신호까지 수학적으로 해석하였고, 시스템의 자기상관과 상호상관에 미치는 영향을 모의실험을 통해 분석하였다. 커플러 수 증가에 따라 나타나는 삼차신호들이 간섭신호 형태가 되어 peak to side-lobe ratio를 감소키기는 요인으로 작용됨을 정량적으로 확인하였다. 간섭신호들의 크기는 ${\alpha}$(coupling coefficient)값이 커질수록 증가하였으며, 커플러 개수(N)=5, ${\alpha}$=0.5인 조건에서 삼차신호까지 고려한 peak to side-lobe ratio는 3.75 dB까지 열화됨을 밝혔다. 또한, 일차신호의 main-lobe세기에 의해 수신기의 임계레벨을 결정한다면, 삼차신호에 의해 증가된 side-lobes세기 때문에 SCAE와 SCAD를 이용한 광 CDMA system에 다중 접속할 수 있는 사용자 수가 제한됨을 알 수 있었다.

• 한국지반신소재학회논문집
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• 제15권2호
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• pp.45-54
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• 2016

본 연구에서는 증가하는 도심지 정체와 신설터널 건설에 따른 여러 제약의 문제를 해소하는 방안으로 향후 국내에서 수요가 증가할 것으로 전망되는 대심도 지하도로를 복층터널로 건설시 본선 복층터널에서 단층터널로 분기가 되는 구간의 터널 거동과 본선과 분기터널간의 필라 안정성을 수치해석을 통하여 검토하였다. 이를 위해 본선터널과 분기터널의 이격거리를 본선터널의 직경(D)을 기준으로 0.1D부터 2.0D까지 변화하는 조건과 지반조건을 암반등급에 따라 1~5등급으로 변화하는 조건들에 대하여 유한요소해석을 수행하였으며, 터널의 전체적인 거동과 함께 그 결과를 기반으로 기존의 경험식 등을 이용한 안전율, 강도-응력비, 그리고 간섭체적비를 산정하여 굴착에 따른 안정성 영향을 검토하였다. 검토 결과 수치해석결과를 이용해 산정된 강도-응력비와 간섭체적비는 경험식을 이용한 방법에 비해 암반강도보다는 이격거리가 안정성에 더욱 크게 영향을 미침을 나타내었고, 그에 따라 본선과 분기터널의 이격거리가 0.7D 이하인 경우는 본선과 분기터널을 모자 형태의 하나의 대단면으로 시공하는 것이 안정성 측면에서 유리한 것으로 판단되었다.

• Asian Pacific Journal of Cancer Prevention
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• 제13권7호
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• pp.3367-3371
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• 2012

Objective: To investigate the effects of histone deacetylase 6 (HDAC6) siRNA on cell proliferation and cell apoptosis of the HeLa cervical carcinoma cell line and the molecular mechanisms involved. Methods: Division was into three groups: A, the untreated group; B, the control siRNA group; and C, the HDAC6 siRNA group. Lipofectamine 2000 was used for siRNA transfection, and Western blot analysis was used to determine the protein levels. Cell proliferation and apoptosis were characterized using a CCK-8 assay and flow cytometry, respectively. Results: HDAC6 protein expression in the HDAC6 siRNA-transfection group was significantly lower (P < 0.05) than in the untreated and control siRNA groups. The CCK-8 kit results demonstrated that the proliferation of HeLa cells was clearly inhibited in the HDAC6 siRNA transfection group (P < 0.05). In addition, flow cytometry revealed that the early apoptotic rate ($26.0%{\pm}0.87%$) was significantly elevated (P < 0.05) as compared with the untreated group ($10.6%{\pm}1.19%$) and control siRNA group ($8.61%{\pm}0.98%$). Furthermore, Western blot analysis indicated that bcl-2 protein expression in the HDAC6 siRNA-transfection group was down-regulated, whereas the expression of p21 and bax was up-regulated. Conclusion: HDAC6 plays an essential role in the occurrence and development of cervical carcinoma, and the down-regulation of HDAC6 expression may be useful molecular therapeutic method.

• Asian Pacific Journal of Cancer Prevention
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• 제15권5호
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• pp.2291-2295
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• 2014

Although nucleolar protein nucleostemin (NS) is essential for cell proliferation and early embryogenesis and expression has been observed in some types of human cancer and stem cells, the molecular mechanisms involved in mediation of cell proliferation and cell cycling remains largely elusive. The aim of the present study was to evaluate NS as a potential target for gene therapy of human breast carcinoma by investigating NS gene expression and its effects on SKBR-3 cell proliferation and apoptosis. NS mRNA and protein were both found to be highly expressed in all detected cancer cell lines. The apoptotic rate of the pcDNA3.1-NS-Silencer group ($12.1-15.4{\pm}3.8%$) was significantly higher than those of pcDNA3.1-NS ($7.2-12.0{\pm}1.7%$) and non-transfection groups ($4.1-6.5{\pm}1.8%$, P<0.01). MTT assays showed the knockdown of NS expression reduced the proliferation rate of SKBR-3 cells significantly. Matrigel invasion and wound healing assays indicated that the number of invading cells was significantly decreased in the pcDNA3.1-NS-siRNA group (P<0.01), but there were no significant difference between non-transfected and over-expression groups (P>0.05). Moreover, RNAi-mediated NS down-regulation induced SKBR-3 cell G1 phase arrest, inhibited cell proliferation, and promoted p53 pathway-mediated cell apoptosis in SKBR-3 cells. NS might thus be an important regulator in the G2/M check point of cell cycle, blocking SKBR-3 cell progression through the G1/S phase. On the whole, these results suggest NS might be a tumor suppressor and important therapeutic target in human cancers.

• Asian Pacific Journal of Cancer Prevention
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• 제17권7호
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• pp.3139-3145
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• 2016

Cancer is the leading cause of morbidity and mortality worldwide, characterized by irregular cell growth. Cytotoxicity or killing tumor cells that divide rapidly is the basic function of chemotherapeutic drugs. However, these agents can damage normal dividing cells, leading to adverse effects in the body. In view of great advances in cancer therapy, which are increasingly reported each year, we quantitatively and qualitatively evaluated the papers published between 1981 and December 2015, with a closer look at the highly cited papers (HCPs), for a better understanding of literature related to cytotoxicity in cancer therapy. Online documents in the Web of Science (WOS) database were analyzed based on the publication year, the number of times they were cited, research area, source, language, document type, countries, organization-enhanced and funding agencies. A total of 3,473 publications relevant to the target key words were found in the WOS database over 35 years and 86% of them (n=2,993) were published between 2000-2015. These papers had been cited 54,330 times without self-citation from 1981 to 2015. Of the 3,473 publications, 17 (3,557citations) were the most frequently cited ones between 2005 and 2015. The topmost HCP was about generating a comprehensive preclinical database (CCLE) with 825 (23.2%) citations. One third of the remaining HCPs had focused on drug discovery through improving conventional therapeutic agents such as metformin and ginseng. Another 33% of the HCPs concerned engineered nanoparticles (NPs) such as polyamidoamine (PAMAM) dendritic polymers, PTX/SPIO-loaded PLGAs and cell-derived NPs to increase drug effectiveness and decrease drug toxicity in cancer therapy. The remaining HCPs reported novel factors such as miR-205, Nrf2 and p27 suggesting their interference with development of cancer in targeted cancer therapy. In conclusion, analysis of 35-year publications and HCPs on cytotoxicity in cancer in the present report provides opportunities for a better understanding the extent of topics published and may help future research in this area.

• Journal of Nutrition and Health
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• 제25권7호
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• pp.555-568
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• 1992

This study was to compare the effects of dietary n-6 and n-3 fatty acids and fat unsaturation on plasma lipids and chemical composition of VLDL and LDL fraction and lipogenic enzymes activity in rat liver under the conditions providing 1) a similar amount of n-6, n-3 fatty acids(LA, ALA, EPA+DHA) in diets and 2) the various degree of fat unsaturation. Male Sprague-Dawley rats weighing 420g were treated for 6-n with six experimental diets providing 25% of energy as fat and which were different only in fatty acid composition. The fats used for a source of each fatty acid were beet tallow for saturated fatty acid corn oil for n-6 linoleic acid(LA) perilla oil for n-3 $\alpha$-linolenic acid(ALA) and fish oil n-3 eicosapentaenoic acid (EPA) and n-3 docosahexaenoic acid(DHA). Plasma cholesterol level was increased by corn oil to compare with beef tallow but was decreased by perilla oil or fish oil. Plasma TG level was significantly decreased by perilla oil or fish oil. Fish oil significantly reduced the level of HDL-Chol and the proportion of Chol in LDL fraction and that of TG in vVLDL fraction. Overall there was a singificant negative correlation between the level of each plasma lipid(Chol TG, VLDL-TG, LDL-C) and the degree of fat unsaturation. However this rerlationship is not always true when compared the hypolipidemic effect of each fatty acid at a similar level of fat unsaturation. There was a trend such taht glucose 6-P dehydrogenase 6-phosphogluconate dehydrogenase and malic enzyme activites were reduced by n-3 fatty acids. Perilla oil significantly increased the incorporation of c20:5 and c22:5 into liver tissue and fish oil suignificantly increased the incorporation of c20:5, c22:6 into liver tissue and the effect of long chain n-3 fatty acid incorporation was greater by fish oil. therefore the hypotriglyceridemic effect of n-3 fatty acid could be resulted from the interference of hepatic lipogenesis by long-chain n-3 fatty acids and the reduced proportion of TG in VLDL fraction and its effect was greater by n-3 EPA+DHA than n-3 ALA even though plasma Chol and TG levels were also influenced by the degree of dietary fat unsaturation.

• Journal of Advanced Marine Engineering and Technology
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• 제41권3호
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• pp.209-215
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• 2017

대형 부유식 파력-해상풍력 복합발전시스템은 정 사각형(폭 150m) 부유식 플랫폼 컬럼 상부에 4기의 3MW 풍력터빈이 설치된다. 전방 풍력터빈으로부터 발생되는 후류는 터빈배치에 따라 후방 풍력터빈의 출력성능과 하중특성에 불리한 영향을 미치므로 후류간섭에 대한 유동해석을 통해 최적배치설계가 실시되어야 한다. 본 논문에서는 플랫폼 배치조건($0^{\circ}$, $22.5^{\circ}$, $45^{\circ}$) 변화에 따른 개별 풍력터빈의 출력특성 및 연간에너지생산량을 확인하기 위해 풍속변화(8m/s, 11.7m/s, 19m/s 25m/s)에 대한 비정상상태 CFD 해석을 실시하였다. 레일리분포를 적용한 연간에너지생산량 계산결과는 각 배치조건에 따라 다르게 나타났으며, 해석결과에 근거하여 후류손실이 최소화 될 수 있는 최적 배치설계를 제안하였다.

• 분석과학
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• 제23권3호
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• pp.240-246
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• 2010

Salicylaldoxime (SA)이 내포되어 있는 미세결정 p-dichlorobenzene 흡착제(p-dichlorobenzene-SA)를 이용하여 시료에 흔적량으로 들어있는 Cu(II)를 예비농축시킨 후 불꽃 원자흡수분광법으로 정량하는 방법을 개발하였다. 시료 용액의 pH, 킬레이트제 salicylaldoxime의 양, 흡착제 p-dichlorobenzene-SA의 양 그리고 시료 용액의 흐름속도 등 여러 실험조건을 최적화하였다. 여러 공존이온에 대한 방해효과를 조사한 결과, $CN^1$가 다른 이온들보다 비교적 심하게 방해하였다. $1\;{\mu}g\;mL^{-1}\;CN^-$에 의한 방해효과는 Ni(II)를$20\;{\mu}g\;mL^{-1}$ 되도록 조절한 결과, 완전히 제거할 수 있었다. 본 방법으로 얻은 직선 감응범위, 상관계수($R^2$) 및 검출한계는 각각 $3.0\sim100\;ng\;mL^{-1}$, 0.9901, 및 $3.1\;ng\;mL^{-1}$ 이었다. 본 방법을 실제 시료에 적용가능 여부를 확인하기 위해 수용액 시료(폐수, 저수지물 및 시냇물)와 식품시료(오렌지 쥬스, 생계란 및 탈지분유)에 응용하였다. 회수율은 93~104% 범위이었으며, ICP-MS를 이용하여 직접 측정한 값과 본 방법으로 측정한 값을 비교한 결과, 95% 신뢰수준에서 잘 일치하였다. 본 방법을 쌀가루 인증표준물질(certified reference material, CRM) 시료에 응용해 보았으며 좋은 결과를 얻었다. 실험 결과들로부터 본 방법은 여러 실제 시료에 들어있는 Cu(II)를 농축 및 정량하는데 응용할 수 있음을 확인하였다.

• Journal of Nutrition and Health
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• 제41권3호
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• pp.242-253
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• 2008

It is a common clinical practice to recommend taking iron supplements for pregnant women during gestation. Although it is required to ensure adequate iron stores during pregnancy, there has been some debate over the interference effects of excessive iron load, because it is possible to compete in the transport in the intestine and placenta and in binding to serum proteins of other trace minerals. In this study, maternal and neonatal serum concentrations of Fe, Zn, Cu, Se, Cr, Mn, and Co were assessed along with maternal Fe intakes. A total of 124 pregnant women and their term neonates participated voluntarily in this research. The women were divided into one of the three groups {high Fe intake (HFI), median Fe intake (MFI), and low Fe intake (LFI)} by their total Fe intakes and one of the two groups (Anemic and Normal) by their Fe nutritional status. All the data were compared among the three groups and between the two groups also. Total Fe intakes of HFI, MFI, and LFI groups were 140.8 ${\pm}$ 76.1, 68.0 ${\pm}$ 11.2, and 30.2 ${\pm}$ 8.6 mg/day, respectively. Those of Anemic and Normal groups were 90.1 ${\pm}$ 74.8 and 86.6 ${\pm}$ 46.8 mg/day, respectively. Maternal Hb concentration and Hct were not significantly different among HFI, MFI, and LFI groups but those were significantly different between Anemic and Normal groups. However, neonatal Hb concentration was not significantly different among HFI, MFI, and LFI groups and between Anemic and Normal groups either. Maternal serum Fe concentrations of the three groups, HFI, MFI, and LFI, were similar but that of Anemic group was significantly lower compared to Normal group. However, there was no significant difference in neonatal serum Fe concentrations among the three groups and between the two groups either. Serum concentrations of the other trace minerals in both mothers and neonates were not significantly different among HFI, MFI, and LFI groups and between Anemic and Normal groups. In addition, in the maternal serum, Fe concentration was positively correlated to Zn and Se concentration, respectively. As for the neonatal serum, Fe concentration showed a positive correlation to Zn, Cu, Mn, Se, and Co concentration, respectively. No trace mineral concentration was found to correlate negatively to Fe concentration in both maternal and neonatal serum, The results in this study indicate that Fe intakes of pregnant women, even if it is considerably above the level of estimated average requirement (EAR), may not affect serum Fe concentration in both mothers and neonates. In addition it might not influence adversely on the availability of other trace minerals including Zn and Cu in both mothers and neonates.