• Title/Summary/Keyword: Intensity level

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Proteomic Analysis of Protein Expression Patterns Associated with Astaxanthin Accumulation by Green Alga Haematococcus pluvialis (Chlorophyceae) Under High Light Stress

  • Kim Jeong-Dong;Lee Woo-Sung;Kim Beob-Min;Lee Choul-Gyun
    • Journal of Microbiology and Biotechnology
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    • v.16 no.8
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    • pp.1222-1228
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    • 2006
  • Two kinds of Haematococcus pluvialis cells (green vegetative cells cultivated under optimal cell culture conditions and red cyst cells maintained under high light stress conditions to induce astaxanthin production) were used to investigate the protein expression profiles by two-dimensional electrophoresis, image analysis, and peptide mass fingerprinting. The cellular accumulation of astaxanthin was evident after exposure to high light intensity and reached the maximum cellular level after 78 h of high light stress. In a 2-D electrophoresis analysis, 22 proteins were upregulated over 2-fold in the red cyst cells when compared with the green vegetative cells and selected for further analysis by chemically assisted fragmentation (CAF)-MALDI-TOF sequencing to identify the protein functions. Among 22 different spots, several key enzymes specific to the carotenoid pathway, including isopentenyl pyrophosphate isomerase (IPP) and lycopene $\beta$-cyclase, appeared in H. pluvialis after exposure to high light intensity. Therefore, IPP and lycopene $\beta$-cyclase would appear to be involved with carotenoid accumulation in the cytoplasm, as these peptides were preferentially upregulated by high light intensity preceding an increase in carotenoid, and only these forms were detected in the red cyst cells.

Measurement of soot concentration in flames using laser-induced incandescence method (레이저 가열 측정법을 이용한 화염 내 매연 농도 측정)

  • Jurng, Jong-Soo
    • Journal of the Korean Society of Combustion
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    • v.1 no.1
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    • pp.75-82
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    • 1996
  • Laser induced incandescence, LII, recently developed technique for measuring soot concentration in flames, can overcome most of limitations of conventional laser extinction measurement. In this study, experiments were performed to investigate the effect of laser intensity, detection wavelength, and also laser beam quality on both LII signal at a particular position and peak-to-centerline LII signal ratio. The results of LII signal with increasing laser intensity shows its near-independence of laser intensity once threshold level of laser intensity has been reached. However, this near-independence depends on laser beam quality and the incident optical setup. The peak-to-centerline LII signal ratio slowly but continuously increases with laser power. This fact is due to the dependence of LII signal on particle mean diameter. LII signal is attenuated during it passes through the flame containing soot particles. The attenuation rate is inversely proportional to detection wavelength. In this study, LII signal at 680 nm band is 10% greater than the signal at 400 nm band.

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Auditory Evoked Skin Potential in Normal Subjects (정상 성인에서 청성유발 피부전위)

  • Heo, Seung-Deok;Jung, Dong-Keun;Suh, Duk-Joon;Kim, Gwang-Nyeon;Kim, Gi-Ryon;Kang, Myung-Koo;Kim, Lee-Suk
    • Speech Sciences
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    • v.12 no.2
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    • pp.81-88
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    • 2005
  • Electrodermal activity(EDA) is a bio-electric signal which occurs at the skin surface during the sweating. EDA reflects the activity of the sympathetic axis of the autonomic nervous system. EDA is associated with the eccrine sweat gland at the palmar and plamar surface. This study was aimed to characterize the relationship between EDA and auditory stimulus intensities. Acoustic stimulus used in this study were 500 Hz, 1 kHz, 2 kHz of narrow band noise, which were representative of speech frequencies in audible range. Stimulus intensity between 90 and 30 dB in 10 dB within dynamic range. After deriving the minimum stimulus intensity(threshold of skin potential) which elicited skin potential, and then the latency and amplitude were derived from waveform of skin potential, each latency and amplitude were compared to stimulus intensity. The waveform of skin potential were recorded stably, and the threshold of skin potential appeared nearly the hearing threshold level of the participant. The latency was decreased and the amplitude was increased according to the increase of the stimulus intensity. These results suggest that auditory evoked skin potential can be applicable to auditory assessment and audiological diagnosis tool.

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Rank-Based Nonlinear Normalization of Oligonucleotide Arrays

  • Park, Peter J.;Kohane, Isaac S.;Kim, Ju Han
    • Genomics & Informatics
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    • v.1 no.2
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    • pp.94-100
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    • 2003
  • Motivation: Many have observed a nonlinear relationship between the signal intensity and the transcript abundance in microarray data. The first step in analyzing the data is to normalize it properly, and this should include a correction for the nonlinearity. The commonly used linear normalization schemes do not address this problem. Results: Nonlinearity is present in both cDNA and oligonucleotide arrays, but we concentrate on the latter in this paper. Across a set of chips, we identify those genes whose within-chip ranks are relatively constant compared to other genes of similar intensity. For each gene, we compute the sum of the squares of the differences in its within-chip ranks between every pair of chips as our statistic and we select a small fraction of the genes with the minimal changes in ranks at each intensity level. These genes are most likely to be non-differentially expressed and are subsequently used in the normalization procedure. This method is a generalization of the rank-invariant normalization (Li and Wong, 2001), using all available chips rather than two at a time to gather more information, while using the chip that is least likely to be affected by nonlinear effects as the reference chip. The assumption in our method is that there are at least a small number of non­differentially expressed genes across the intensity range. The normalized expression values can be substantially different from the unnormalized values and may result in altered down-stream analysis.

A study on the growth behaviors of surface fatigue crack initiated from a small-surface defect of 2024-T3 and brass (2024-T3 및 황동의 작은 표면결함재의 피로균열 성장특성에 관한 연구)

  • 서창민;오명석
    • Journal of Ocean Engineering and Technology
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    • v.10 no.1
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    • pp.53-64
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    • 1996
  • In this paper, rotating bending fatigue tests have been carried out to investigate the growth behabiors of surface fatigue crack initiated from a small artificial surface defect, that might exist in real structures, on 2024-T3 and 6:4 brass. The test results are analysed in the viewpoints of both strength of materials and fracture mechanics, it can be concluded as follows. The effect of a small artificial surface defect upon the fatigue strength is very large. The sensitivity of 2024-T3 on the defect is higher than that of 6:4 brass. The growth behavior of the surface fatigue crack of 2024-T3 is different from that of 6:4 brass. The growth rate of the surface fatigue crack of 2024-T3 is considerably rapid in the early stage of the fatigue life and apt to decrease in the later stage. It was impossible to establish a unifying approach in the analysis of crack growth begabior of 2024-T3 and 6:4 brass using the maximum stress intensity factor because of their dependence on stress level. But if the elastic strain and cyclic total strain intensity factor range were applied to obtain the growth rate of surface fatigue cracks of the materials, the data were found to be nearly coincided.

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On the Characteristics of the Precipitation Patterns in Korea Due to Climate Change

  • Park, Jong-Kil;Seong, Ihn-Cheol;Kim, Baek-Jo;Jung, Woo-Sik;Lu, Riyu
    • Journal of Environmental Science International
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    • v.23 no.1
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    • pp.25-37
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    • 2014
  • In the present study, we analyzed precipitation patterns and diurnal variation trends of hourly precipitation intensity due to climate change. To that end, we used the hourly precipitation data obtained from 26 weather stations around South Korea, especially Busan, from 1970 to 2009. The results showed that the hourly precipitation was concentrated on a specific time of day. In particular, the results showed the so-called "morning shift" phenomenon, which is an increase in the frequency and intensity of hourly precipitation during the morning. The morning shift phenomenon was even more pronounced when a higher level of hourly precipitation intensity occurred throughout the day. Furthermore, in many regions of Korea, including Busan, this morning shift phenomenon became more prevalent as climate change progressed.

An Analysis of Determinants of Elderly Medical Costs Inflation Using Deterministic Model (결정론적 모형에 의한 노인진료비 상승요인 분석)

  • Yu, Seung-Hum;Sohn, Myong-Sei;Park, Eun-Cheol
    • Journal of Preventive Medicine and Public Health
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    • v.27 no.1 s.45
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    • pp.135-144
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    • 1994
  • The purpose of this study compares determinants of eldery medical cost inflation with those of other age groups by analysing aggregated data with a deterministic model. The deterministic model of per capita medical cost inflation consists of increases in price, intensity of services, and medical utilization. We used a time series data($1985{\sim}1991$) from National Medical Insurance and analyzed by age groups. In total population, the average increase rates of inpatient and outpatient medical costs were respectively 9.5% and 8.8% during 6 years and the major cause of inflation was the increase in service intensity in both of inpatient and outpatient cases. But in the population of 65 years old and over, the average increase rates of inpatient and outpatient medical costs were respectively 13.8% and 14.8% and the major cause of inflation was the increase in per-capita medical utilization in both of inpatient and outpatient cases. Also, the increase in service intensity of 65 years old and over was the highest of other age groups. This pattern was similar during study periods. We concluded that the level of medical cost-inflation and the determinants in eldery was the highest-especially in per capita medical utilization, therfore, the inflation of medical costs in eldery will be higher than other age groups for the furture in Korea.

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The Characteristics of Local Reaction Intensity with Changing Combustor Pressure in the Swirl-stabilized Flame (스월화염에서 연소실 압력 변동에 의한 국소 반응강도의 특성)

  • Noh, Young-Gu;Kim, Jong-Ryul;Kim, Tae-Hyung;Seo, Sang-Il;Kim, Sung-Chul;Na, Jong-Moon;Choi, Gyung-Min;Kim, Duck-Jool
    • Journal of the Korean Society of Combustion
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    • v.14 no.4
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    • pp.41-47
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    • 2009
  • An experimental study was performed to confirm the effect of the changing combustor pressure(-30~30 kpa), combustion characteristics were investigated by measuring the local chemiluminescence intensity, the local temperature distribution and emission. In order to investigate combustion ones, the combustor pressure index($P^*$) was controlled in the range of 0.7~1.3 for each equivalence ratio in the present combustion system, where $P^*$ is defined as the ratio of absolute pressure to atmospheric one. The local mean temperature showed the uniform distributions for lower pressure index, which increased with increasing equivalence ratio. The mean $OH^*$ chemiluminescence intensity, showed high level for lower pressure index for ${\Phi}{\get}1.0$ conditions. EINOx decreased with decreasing pressure index for overall equivalence ratio conditions.

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Influence of Light Intensity on the Blind-side Hypermelanosis in Starry Flounder Plathchthys stellatus (강도다리(Platichthys stellatus) 흑화발현에 미치는 조도의 영향)

  • KIM, Won Jin;SHIN, Yun Kyung
    • Journal of Fisheries and Marine Sciences Education
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    • v.28 no.4
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    • pp.1098-1106
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    • 2016
  • To study the influence of light intensity on blind-side hypermelanosis of starry flounder, Platichthys stellatus, we compared growth, glucose, cortisol, ratio of malpigmented blind-side area, ambicolored fish ratio and expression of mch mRNA for 60 days in 0 lux(darkness) and 20 lux(low light density). The test was done in duplicate at 50 fish/tank with the selected ordinary juvenile flounder. The rearing was performed in FRP aquarium tanks ($H100cm{\times}L100cm{\times}W100cm$). Growth was higher in the 0 lux. The ratio of malpigmented blind-side area and ambicolored fish ratio were significantly higher at the 0 lux than at the 20 lux. The result suggests that 20 lux could inhibit the hypermelanosis. The expression level of MCH 1, 2 mRNA was significantly lower in 0 lux, indicating that MCH 1, 2 and light intensity are related to blind-side hypermelanosis of starry flounder.

The Obstacle Avoidance Algorithm of Mobile Robot using Line Histogram Intensity (Line Histogram Intensity를 이용한 이동로봇의 장애물 회피 알고리즘)

  • 류한성;최중경;구본민;박무열;윤경섭;윤석영
    • Proceedings of the IEEK Conference
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    • 2002.06d
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    • pp.331-334
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    • 2002
  • In this paper, we present two types of vision algorithm that mobile robot has CCD camera. for obstacle avoidance. This is simple algorithm that compare with grey level from input images. Also, The mobile robot depend on image processing and move command from PC host. we has been studied self controlled mobile robot system with CCD camera. This system consists of digital signal processor, step motor, RF module and CCD camera. we used wireless RF module for movable command transmitting between robot and host PC. This robot go straight until recognize obstacle from input image that preprocessed by edge detection, converting, thresholding. And it could avoid the obstacle when recognize obstacle by line histogram intensity. Host PC measurement wave from various line histogram each 20 Pixel. This histogram Is ( x , y ) value of pixel. For example, first line histogram intensity wave from ( 0, 0 ) to ( 0, 197 ) and last wave from ( 280, 0 ) to ( 280, 197 ). So we find uniform wave region and nonuniform wave region. The period of uniform wave is obstacle region. we guess that algorithm is very useful about moving robot for obstacle avoidance.

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