• Title/Summary/Keyword: Intensity level

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Curcumin Attenuates Hydrogen Peroxide Induced Oxidative Stress on Semen Characteristics during In Vitro Storage of Boar Semen

  • Jang, Hyun-Yong;Kim, Young-Han;Cheong, Hee-Tae;Kim, Jong-Taek;Park, In-Chul;Park, Choon-Keun;Yang, Boo-Keun
    • Reproductive and Developmental Biology
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    • v.33 no.2
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    • pp.99-105
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    • 2009
  • Curcumin is a major active component of the food flovour tumeric. It has been used for the treatment of many diseases such as inflammatory and infectious diseases, cancer and other disease due to its antioxidant properties. Curcumin is a powerful scavenger of many free radicals such as superoxide anion, hydroxyl radical and nitric oxide. The objective of this study was to investigate the antioxidative effects of curcumin against hydrogen peroxide on semen quality during in vitro storage of boar semen. The sperm treated with different concentration of curcumin (1, 5 and 10 ${\mu}M$) in the presence or absence of hydrogen peroxide (250 ${\mu}M\;H_2O_2$) were incubated for 3, 6 and 9 hr at $37^{\circ}C$ and analyzed sperm characteristics such as motility, membrane integrity (MI), lipid peroxidation (LPO), reactive oxygen species (ROS) and DNA fragmentation (DF). The sperm motility and MI in $H_2O_2$ treated group ($47.8%{\pm}6.8$ and $24.8%{\pm}2.2$) were significantly decreased when compare to curcumin treated group ($79.8%{\pm}2.7$ and $34.6%{\pm}1.0$, respectively) irrespective of incubation periods(p<0.05). The LPO of spermatozoal plasma membrane was measured by thiobarbituric acid (TBA) reactions for malondialdehyde (MDA), MDA level in control ($11.6{\pm}0.6\;nmol/L{\times}10^6$) and curcumin groups ($10.7{\pm}0.3\;nmol/L{\times}10^6$) were lower than those of curcumin plus $H_2O_2$ ($17.1{\pm}0.8\;nmol/L{\times}10^6$) or $H_2O_2$ group ($22.5{\pm}1.9\;nmol/L{\times}10^6$) from 3 to 9 hr incubation periods. The DF by sperm chromatin dispersion (SCD) test and ROS production measured by 2',7'-dichlorofluorescein (DCF) fluorescence intensity were no significantly difference through all experimental groups (p>0.05). Correlation among evaluation methods for sperm quality, motility vs MI and DF vs ROS was positively correlated while motility vs DF and ROS vs LPO were negatively correlated in all treatment groups. These results demonstrate that curcumin can effectively improve the sperm quality during in vitro storage of boar semen through its hydrogen peroxide scavenging mechanism as an antioxidant.

Effects of Dietary Supplementation of Taurine, Carnitine or Glutamine on Endurance Exercise Performance and Fatigue Parameters in Athletes (타우린, 카르니틴 또는 글루타민 섭취가 운동선수의 지구력운동 수행능력 및 혈중 피로요소에 미치는 영향)

  • 이해미;백일영;박태선
    • Journal of Nutrition and Health
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    • v.36 no.7
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    • pp.711-719
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    • 2003
  • The effects of taurine, carnitine or glutamine supplementation on endurance exercise performance along with related fatigue factors were evaluated in male college students in the Department of Physical Education, who's maximal oxygen consumption rates (VO$_2$max) were equivalent to those of endurance athletes. Twenty four subjects were randomly divided into 4 groups (n=6), and given placebo, taurine (4 g/day), carnitine (4 g/day), or glutamine (4 g/day) tablets for 2 weeks. Subjects could run 6.9 min or 9.0 min longer until exhausted on a treadmill at the intensity of 75% VO$_2$max following taurine or camitine supplementation for 2 weeks, respectively, compared to the value measured prior to each supplementation. Glutamine or placebo supplementation did not improve the endurance exercise performance based on the running time until exhausted on a treadmill. Serum lactate concentrations measured 1 hr after the initiation of the endurance exercise, as well as at all-out state tended to be decreased by taurine, carnitine, or glutamine supplementation, and were significantly lowered (43% decrease) by carnitine supplementation (p < 0.05). Taurine supplementation significantly reduced the serum inorganic phosphorus concentration measured at all-out state (14% decrease, p < 0.05), while carnitine supplementation significantly lowered the resting state serum inorganic phosphorus level (20% decrease, p < 0.05). Taurine (32% reduction) or carnitine (23% reduction) supplementation significantly decreased serum ammonia concentration measured at all-out state (p < 0.05). From these results, 4 g/day of taurine or carnitine supplementation appears to improve the endurance exercise performance and related human fatigue factors.

Metal Corrosion Mechanism by Sulfate-reducing and Iron-oxidizing Bacteria in Saline System and its Optimal Inactivation (염수계 철산화균 및 황환원균에 의한 금속 부식 및 최적 제어 방안)

  • Sung, Eun-Hae;Han, Ji-Sun;Kim, Chang-Gyun
    • Journal of Korean Society of Environmental Engineers
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    • v.30 no.8
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    • pp.798-807
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    • 2008
  • Due to economic impairment derived from metal corrosion of pumping station installed around coastal area, it was needed for related cause-effect to be investigated for understanding practical corrosion behavior and providing proper control. This research was thus carried out to determine whether the microbe can influence on metal corrosion along with its control in the laboratory. For this study, groundwater was sampled from the underground pump station(i.e. I Gas Station) where corrosion was observed. Microbial diversity on the samples were then obtained by 16S rDNA methods. From this, microbial populations showing corrosion behaviors against metals were reported as Leptothrix sp.(Iron oxidizing) and Desulfovibrio sp.(Sulfur reducing) Iron oxidizing bacteria were dominantly participating in the corrosion of iron, while sulfate reducing bacteria were more preferably producing precipitate of iron. In case of galvanized steel and stainless steel, iron oxidizing bacteria not only enhanced the corrosion, but also generated its scale of precipitate. Sulfate reducing bacteria had zinc steel corroded greater extent than that of iron oxidizing bacteria. In the inactivation test, chlorine or UV exposure could efficiently control bacterial growth. However as the inactivation intensity being increased beyond a threshold level, corrosion rate was unlikely escalated due to augmented chemical effect. It is decided that microbial corrosion could be differently taken place depending upon type of microbes or materials, although they were highly correlated. It could be efficiently retarded by given disinfection practices.

Comparisons of Color Spaces for Shadow Elimination (그림자 제거를 위한 색상 공간의 비교)

  • Lee, Gwang-Gook;Uzair, Muhammad;Yoon, Ja-Young;Kim, Jae-Jun;Kim, Whoi-Yul
    • Journal of Korea Multimedia Society
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    • v.11 no.5
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    • pp.610-622
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    • 2008
  • Moving object segmentation is an essential technique for various video surveillance applications. The result of moving object segmentation often contains shadow regions caused by the color difference of shadow pixels. Hence, moving object segmentation is usually followed by a shadow elimination process to remove the false detection results. The common assumption adopted in previous works is that, under the illumination variation, the value of chromaticity components are preserved while the value of intensity component is changed. Hence, color transforms which separates luminance component and chromaticity component are usually utilized to remove shadow pixels. In this paper, various color spaces (YCbCr, HSI, normalized rgb, Yxy, Lab, c1c2c3) are examined to find the most appropriate color space for shadow elimination. So far, there have been some research efforts to compare the influence of various color spaces for shadow elimination. However, previous efforts are somewhat insufficient to compare the color distortions under illumination change in diverse color spaces, since they used a specific shadow elimination scheme or different thresholds for different color spaces. In this paper, to relieve the limitations of previous works, (1) the amount of gradients in shadow boundaries drawn to uniform colored regions are examined only for chromaticity components to compare the color distortion under illumination change and (2) the accuracy of background subtraction are analyzed via RoC curves to compare different color spaces without the problem of threshold level selection. Through experiments on real video sequences, YCbCr and normalized rgb color spaces showed good results for shadow elimination among various color spaces used for the experiments.

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The Study of HR and Energy Expenditure Change according to Walking Types (걷기유형에 따른 심박수 및 에너지소비량 변화에 관한 연구)

  • Lee, hyung-kook
    • Proceedings of the Korea Contents Association Conference
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    • 2009.05a
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    • pp.405-409
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    • 2009
  • There are many types of walking exercise. We have choice the way according to the walking exercise purpose. Nordic walking(NW) is one of walking types with nordic poles. Also, power walking(PW) has motion of large swing the arm in walking, and dumbbell walking(DW) way is walking with 3-pound dumbbell. This study compared the physiological response(heart rate, energy expenditure) of general walking(GW) to another types(nordic, power, and 3-pound hand weights walking way). Seven apparently male health volunteers between the ages 19 and 24 years participated. Each complete a treadmill test. The tests were assigned randomly, as submaximal walking trials on separate days. Each walking trial was conducted on a level treadmill, for 40 minutes(3-5mile/hour 5min warm-up, 6mile/hour speed for 15min walking and 7mile/hour speed for 15min Jogging exercise, and 5-3mile/hour cool down 5min), at an same pace. Heart rate in beats per minute(bpm), and energy expenditure in kcal per minute(kcal/min) were recorded each minute. Results between trials were compared using repeated measures analysis of variance and Tukey's post hoc tests. In slow walking, it was found that walking with 3-pound hand weight way resulted in and average of ($127.8{\pm}8.27bpm$) the highest score HR, Caloric expenditure($85.4{\pm}14.51kcal/min$), responses compared to regular walking way($117.4{\pm}7.27bpm$ and $70.4{\pm}10.99kcal/min$). Nordic walking way($121.4{\pm}11.74bpm$, and $77.0{\pm}16.83kcal/min$) is second, power walking way($118.5{\pm}9.98bpm$, and $68.7{\pm}20.62kcal/min$) is next. In fast walking, it was found that walking way with 3-pound hand weight resulted in and average of ($160.1{\pm}8.72bpm$) the highest score HR, caloric expenditure($126.1{\pm}13.86kcal/min$), responses compared to regular walking way($148.4{\pm}11.94bpm$, and $109.0{\pm}4.70kcal/min$). Nordic walking way($156.7{\pm}10.82bpm$, and $113.5{\pm}14.51kcal/min$) is second, power walking way($149.7{\pm}12.56bpm$, and $109.2{\pm}17.64kcal/min$) is next. Thus, it is the unavoidable conclusion that, comparing with general walking, 3-pound hand weight walking, nordic walking, and power walking methods have the advantage of high exercise intensity and energy expenditure to meet the purpose of performers without the problem. Furthermore, 3-pound hand weight walking ways were proved to be a useful aerobic exercise method as whole body that achieves high-energy efficiency. To this extent, 3-pound hand weight walking ways can be recommended as a continuous and regulative aerobic exercise for some people.

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Change in Opto-electrical Characteristics in Poly[3-octylthiophene-co-3-(4-fluorophenyl)thiophene] according to the Copolymerization Ratio (Poly[3-octylthiophene-co-3-(4-fluorophenyl)thiophene]에서 공중합 비율에 따른 전기 광학적 특성의 변화)

  • 신선호;정애영;김주현;이후성;김동표
    • Polymer(Korea)
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    • v.25 no.3
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    • pp.399-405
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    • 2001
  • Poly[3-octylthiophene-co-3-(4-fluorophenyl)thiophene]s were synthesized in 2:1, 1:1, and 1:2 mole ratios, and organic electroluminescent devices were fabricated using the copolymers. The opto-electrical properties of the copolymers were studied by PL, EL spectra, I-V, and V-L curves of the organic electroluminescent devices in conjunction with the energy band diagrams which were obtained from the cyclic voltammogram and the electronic absorption spectra. The LUMO energy level of P(OT/FPT)(1:1) is the lowest as -3.35 eV. In the copolymers P(OT/FPT)(2:1) and P(OT/FPT)(1:1) the ${\lambada}_{max}$ in the PL and EL spectra red-shifted as the mole ratio of fluorophenyl group increased while in P(OT/FPT)(1:2) it showed a blue-shift. This indicates that the backbone chain is twisted due to the steric hinderance of the fluorophenyl group leading to shorter ${\pi}$-conjugation length. P(OT/FPT)(1:1) showed the highest EL intensity and the highest power efficiency among the three copolymers. In P(OT/FPT)(1:2) the roughness of the film surface causes unusually high local leakage current leading to the low efficiency of electroluminescence.

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The Effect of Aerobic Exercise and Allium Tuberosum Intake on Blood Lipids, MDA and Antioxidant Enzyme in Rats (유산소 운동과 부추섭취가 혈중지질, 지질과산화 및 항산화효소에 미치는 영향)

  • Baek, Yeong-Ho;Lee, Sang-Ho
    • Journal of Life Science
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    • v.20 no.2
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    • pp.245-252
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    • 2010
  • The purpose of this study was to investigate the effects of aerobic exercise and Allium tuberosum intake on blood lipids, MDA and antioxidant enzyme in rats. Twenty four male Sprague-Dawley rats, 4 weeks old, were used. Experimental groups were aerobic exercise with Allium tuberosum intake group (A, n=6), aerobic exercise group (B, n=6), Allium tuberosum intake group (C, n=6), and the control group (D, n=6). Aerobic exercise was administered through a treadmill running program (14~15 m/min, $0^{\circ}$ grade, 25~30 min/day, 5 day/wk) and these rats were given 5% Allium tuberosum for 2 wk. The results of this study are as follows: TC and TG didn't show change; groups A, B, C showed a significant increase in HDL-C compared to the D group; groups A, B, and C showed a significant decrease in LDL-C compared to the D group; groups B and C showed a significant decrease in MDA level compared to the D group; groups B and C showed a significant increase in SOD activity compared to the D group; and the A group showed a significant increase in CAT activity compared to the D group. In conclusion, low intensity aerobic exercise and intake of the natural antioxidant Allium tuberosum seem to have the health promoting effect of retarding oxidative stress by decreasing lipid peroxidation.

The Effects of Aerobic Training Intensity Difference on Cardiovascular Disease Pathophysiological Pathways Linking Oxidative Tissue Damage in Obese Children (유산소 훈련 강도 차이가 비만 어린이의 산화적 조직 손상에 의한 심혈관질환 병태생리적 경로에 미치는 영향)

  • Woo, Jin-Hee;Shin, Ki-Ok;Kim, Keun-Soo;Kim, Young-Il;Kang, Sung-Hwun
    • Journal of Life Science
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    • v.20 no.9
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    • pp.1394-1401
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    • 2010
  • The purpose of this study was to examine the effect of 12 wk of aerobic exercise on lipid profiles, antioxidant enzyme activities, oxidative products, and autonomic nervous activity (ANA) in children with obesity. We studied 16 children with obesity and 19 age-matched normal weight controls over a period of 12 wk, during which time moderate intense running exercise was performed. Measurements included peak oxygen uptake, body composition, blood lipid profiles, ox-LDL, 8-OHdG, SOD, GPx activities, total mRNA, and ANA. There were no differences in body weight between periods in the OW group, but body weight increased after 12 wk in OR and CO groups. There were no differences in WHR between periods in the OR and CO groups, but the WHR values decreased after 12 weeks in the OW group. In the obese group, the baseline TG was higher than in the controls (p<0.05), while the ANA level was lower. There were differences in antioxidant enzyme gene expressions between periods in all groups. In conclusion, oxidative damage and antioxidant enzyme activities in obese children were found to be similar to those of normal weight children. However, TG was higher and ANA was lower in obese children than in normal weight children. These results indicated that increased TG and decreased ANA levels begins in childhood in obese patients. Also, regular aerobic exercise may modify the antioxidant enzyme gene expression in early life.

Quality Characteristics of Tomato Sauce added with Various Thickening Agent (농후제를 달리한 토마토소스의 품질 특성)

  • Kim, Young-Jun;Kim, Ki-bbeum;Park, Ki-hong;Choi, Soo-keun
    • Culinary science and hospitality research
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    • v.22 no.7
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    • pp.100-111
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    • 2016
  • Tomato sauce were prepared with five different thickening agents including roux (TR), non-glutinous rice powder (TN), glutinous rice powder (TG), potato starch (TP) and tapioca starch (TT) to examine proximate composition (moisture, carbohydrate, crude protein, crude fat, crude ash, crude ash), calorie, color value, pH, salinity, $^{\circ}Brix$, reducing sugar, viscosity and sensory test (attribute difference, acceptance). The results were as follows: Moisture, carbohydrate content were the lowest while crude fat and calorie were the highest in TR (roux). On the other hand, moisture, carbohydrate content were the highest while crude protein, crude fat and calorie were the lowest in TP (potato starch) and TT (tapioca starch). Using potato starch and tapioca starch are supposed to be prepared low-fat, low-calorie tomato sauce. L value was the highest in TN (non-glutinous rice powder), a value was the highest in TP (potato starch), b values was the highest in TR (roux). pH of tomato sauce showed a range of 5.24 to 5.39. TG (glutinous rice powder) was the highest and TT (tapioca starch) was the lowest in pH. TP (potato starch) was the highest salinity, reducing sugar was the lowest. TG (glutinous rice powder) was the lowest salinity, $^{\circ}Brix$ was the highest. And TR (roux) was the lowest $^{\circ}Brix$, reducing sugar was the highest. In viscosity, TG (glutinous rice powder) was the highest and TT (tapioca starch) was the lowest. The attribute difference test results was the highest in gloss, color intensity, tomato odor, tomato taste, pure taste in TT (tapioca starch) and savory taste, oily taste, thickness, residue was the highest in TR (roux). The preference test results reveal that the appearance, flavor, taste, texture and overall acceptance level was the highest in TP (potato starch) and TT (tapioca starch). The results of this study, tomato sauce prepared potato starch and tapioca starch instead of the traditional roux was higher in sensory acceptability. Recently, people is avoided high-fat and high-calorie foods, and potato starch and tapioca starch are confirmed that the tomato sauce can be made of a low-fat and low-calorie.

The Environmental Characteristics and Vegetation Structures of Kirengeshoma Koreana Habitats (나도승마 서식지의 환경적 특성과 식생구조)

  • Cho, Seon-Hee;Kim, Dae-Woo;Kang, Sang-Ho;Jeong, Yeo-Hong;Lee, Sung-Jae;Kim, Joon-Sun
    • Journal of Korean Society of Forest Science
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    • v.102 no.3
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    • pp.446-454
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    • 2013
  • The present study investigated the biological agents and environmental conditions of the habitats of the Kirengeshoma koreana, a class 2 endangered species class according to the South Korean Ministry of Environment (MOE), elucidated the structural characteristics of the forest stands in the habitats with the importance value and the diversity index, conducted comparative analysis of the factors, and explored their relationship with environmental factors. According to the results of direction analysis of natural habitats of the Kirengeshoma koreana, the direction was distributed to the north or the northeast, and the slope amounted to $15-40^{\circ}$, thus demonstrating that the slope has little effect. In addition, the results showed that the distribution of Kirengeshoma koreana populations dramatically increased starting in areas at least 600 m above the sea level and that the relative light intensity was 3.79% on an average. Most populations were native to gorges and crevices between rocks so that they mainly tended to inhabit areas that could see soil loss in times of precipitation. In addition, a considerable number of the populations were close to hiking trails as well, thus leading to concerns about their artificial destruction. The soil of layer a in the main growth space of the Kirengeshoma koreana was analyzed and turned out to be strongly acidic, measuring pH 4.45-4.89 (average: 4.68). All investigation sections were typical natural broad-leaved tree areas, and, judging from the fact that the Kirengeshoma koreana was most likely to appear in colonies of the Mongolian oak tree (Quercus mongolica Fisch. ex Ledeb.), the Mongolian oak tree seems to be the species of tree that has the greatest effect on the inhabitation of the Kirengeshoma koreana.