• Asian-Australasian Journal of Animal Sciences
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• 제17권10호
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• pp.1390-1394
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• 2004

We have achieved efficient transformation system for forage-type tall fescue plants by Agrobacterium tumefaciens. Mature seed-derived embryogenic calli were infected and co-cultivated with each of three A. tumefaciens strains, all of which harbored a standard binary vector pIG121Hm encoding the neomycin phosphotransferase II (NPTII), hygromycin phosphotransferase (HPT) and intron-containing $\beta$-glucuronidase (intron-GUS) genes in the T-DNA region. Transformation efficiency was influenced by the A. tumefaciens strain, addition of the phenolic compound acetosyringone and duration of vacuum treatment. Of the three A. tumefaciens strains tested, EHA101/pIG121Hm was found to be most effective followed by GV3101/pIG121Hm and LBA4404/pIG121Hm for transient GUS expression after 3 days co-cultivation. Inclusion of 100 $\mu$M acetosyringone in both the inoculation and co-cultivation media lead to an improvement in transient GUS expression observed in targeted calli. Vacuum treatment during infection of calli with A. tumefaciens strains increased transformation efficiency. The highest stable transformation efficiency of transgenic plants was obtained when mature seed-derived calli infected with A. tumefaciens EHA101/pIG121Hm in the presence of 100 $\mu$M acetosyringone and vacuum treatment for 30 min. Southern blot analysis indicated integration of the transgene into the genome of tall fescue. The transformation system developed in this study would be useful for Agrobacterium-mediated genetic transformation of tall fescue plants with genes of agronomic importance.

• 천문학논총
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• 제15권spc2호
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• pp.57-64
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• 2000

It is realized that the extraterrestrial matter is in ionized state, plasma, so the matter of this kind behaves as not expected because of its sensitiveness to electric and magnetic fields and its ability to carry electric currents. This kind of subtle change can be observed by an instrument for the magnetic field measurement, the magnetometer usually mounted on the rocket and the satellite, and based on the ground observatory. The magnetometer is a useful instrument for the spacecraft attitude control and the Earth's magnetic field measurements for the scientific purpose. In this paper, we present the preliminary design and the test results of the two onboard magnetometers of KARl's (Korea Aerospace Research Institute) sounding rocket, KSR­III, which will be launched during the period of 2001-02. The KSR-III magnetometers consist of the fluxgate magnetometer, MAG/AIM (Attitude Information Magnetometer) for acquiring the rocket flight attitude information, and of the search-coil magnetometer, MAG/SIM (Scientific Investigation Magnetometer) for the observation of the Earth's magnetic field fluctuations. With the MAG/AIM, the 3-axis attitude information can be acquired by the comparison of the resulting dc magnetic vector fields with the IGRF (International Geomagnetic Reference Field). The Earth's magnetic field fluctuations ranging from 10 to 1,000 Hz can also be observed with the MAG/SIM measurement.

• The Plant Pathology Journal
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• 제23권2호
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• pp.51-56
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• 2007

A plant pathogenic fungus, Gibberella zeae (anamorph: Fusarium graminearum), not only generates economic losses by causing disease on cereal grains, but also leads to severe toxicosis in human and animals through the production of mycotoxins in infected plants. Here, we characterized a histidine auxotrophic mutant of G. zeae, designated Z43R1092, which was generated using a restriction enzyme-mediated integration (REMI) procedure. The mutant exhibited pleiotropic phenotypic changes, including a reduction in mycelial growth and virulence and loss of sexual reproduction. Outcrossing analysis confirmed that the histidine auxotrophy is linked to the insertional vector in Z43R1092. Molecular analysis showed that the histidine requirement of Z43R1092 is caused by a disruption of an open reading frame, designated GzHIS7. The deduced product of GzHIS7 encodes a putative enzyme with an N-terminal glutamine amidotransferase and a C-terminal cyclase domain, similar to the Saccharomyces cerevisiae HIS7 required for histidine biosynthesis. The subsequent gene deletion and complementation analyses confirmed the functions of GzHIS7 in G. zeae. This is the first report of the molecular characterization of histidine auxotrophy in G. zeae, and our results demonstrate that correct histidine biosynthesis is essential for virulence, as well as sexual development, in G. zeae. In addition, our results could provide a G. zeae histidine auxotroph as a recipient strain for genetic transformation using this new selectable marker.

• Journal of Plant Biotechnology
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• 제44권4호
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• pp.388-393
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• 2017

We report an Agrobacterium-mediated transformation procedure optimized for 'Kyoho' that is a major table grapevine cultivar in Korea, and its transgenic plants with antifreeze protein gene of carrot (DcAFP). The full length of DcAFP coding region in accordance with the previous report was isolated from young leaves of carrot and recombined into a plant transformation vector. Ethylene inhibitors such as silver nitrate and aminoethoxyvinylglycin (AVG) supplemented in a co-cultivation medium distinctly increased frequency of shoot regeneration when explants were sub-cultured in a selection medium: particularly ten-fold higher in treatment with 0.1 mg/L AVG than one without ethylene inhibitor. Among various antibiotics and their concentrations, the combination of 150 mg/L cefotaxime plus 150 mg/L $Clavamox^{TM}$ was selected for elimination of Agrobacterium cells in addition to minimization of adverse effect on shoot regeneration, while 50 mg/L kanamycin monosulfate effectively suppressed regeneration of non-transgenic shoots. Applying the elucidated culture condition, we finally obtained a total of 5 transgenic 'Kyoho' plantlets with DcAFP, of which integration with the grapevine genome and transcription was confirmed by nucleic acid analyses.

• International Journal of Industrial Entomology and Biomaterials
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• 제2권1호
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• pp.19-26
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• 2001

Transformed Spodoptera frugiperda (Sf9) cells expressing baculovirus very late factor (VLF-1) were constructed by using Autograha nuclear polyhedrosis virus (AcNPV) immediate earthy gene (ie1). Neomycin-resistance gene as a selectable marker was introduced under the control of AcNPV ie1 promoter, and Bombyx mori nuclear polyhedrosis (BmNPV-K1) vlf-1 gene was introduced under the control of the Drosophila heat shock protein gene (hspr70) promoter to yield dual expression plasmid with two independent transcription units. It was transfected into Sf9 cells and cell clones expressing vlf-1 were selected by G4l8 treatment. Genomic DNA from transformed cells was isolated and integration of AcNPV iel harboring vlf-1 was confirmed by PCR using AcNPV iel-specific primers and Southern blot analysis. The transformed cells expressing VLF-1 in an infection-independent manner expressed foreign gene product of recombinant baculovirus in the earlier stage of infection compared with control Sf9 cells. These results suggest the possible to develop highly efficient transformed insect cells for baculovirus expression vector system.

• 대한수의학회지
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• 제53권4호
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• pp.217-224
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• 2013

Transgenic plants have been tested as an alternative host for the production and delivery of experimental oral vaccines. Here, we developed transgenic potatoes that express the major antigenic sites A and D of the glycoprotein S from transmissible gastroenteritis coronavirus (TGEV-$S_{0.7}$) under three expression vector systems. The DNA integration and mRNA expression level of the TGEV-$S_{0.7}$ gene were confirmed in transgenic plants by PCR and northern blot analysis. Antigen protein expression in transgenic potato was determined by western blot analysis. Enzyme-linked immunosorbent assay results revealed that based on a dilution series of Escherichia coli-derived antigen, the transgenic line P-2 had TGEV-$S_{0.7}$ protein at levels that were 0.015% of total soluble proteins. We then examined the immunogenicity of potato-derived TGEV-$S_{0.7}$ antigen in mice. Compared with the wild-type potato treated group and synthetic antigen treated group, mice treated with the potato-derived antigen showed significantly higher levels of immunoglobulin (Ig) G and IgA responses.

• 한국전자파학회논문지
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• 제14권6호
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• pp.630-637
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• 2003

WCDMA 시스템에서 주파수 오차에 의한 단말기 성능 영향을 분석하기 위해 기지국과 이동 단말기 사이의 주파수 오차 발생 원인을 고찰한 후 단말기 수신부에서 주파수 오차를 정정하기 위한 자동 주파수 오차 보상방법을 소개하였다. 시스템 요구 규격 중 자동 주파수 오차 보상 동작에 관련된 성능요구 조건들을 바탕으로 여 기지국과 단말기간 연동시험을 수행하였고 주파수 오차보상 후 단말기 송신 주파수에서 4 Hz의 주파수 차를 얻음으로써 시스템에서 요구하는 주파수 오차 허용량인 $\pm$ 0.1 ppm($\pm$200 Hz)이내의 규격을 만족함을 보였다. 주파수 오차에 의한 송신성능 열화를 에러벡터 크기(EVM) 값으로 확인하였으며 또한 수신 성능 열화를 Ec/Io로 측정하였고, 주파수 오차조정의 정확성을 개선시키기 위한 모뎀 제어신호와 RF 간 인터페이스 관계를 제시하였다.

• 한국산업정보학회논문지
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• 제17권7호
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• pp.139-148
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• 2012

분류기의 앙상블 학습은 여러 개의 서로 다른 분류기들의 조합을 통해 만들어진다. 앙상블 학습은 기계학습 분야에서 많은 관심을 끌고 있는 중요한 연구주제이며 대부분의 경우에 있어서 앙상블 모형은 개별 기저 분류기보다 더 좋은 성과를 내는 것으로 알려져 있다. 본 연구는 부도 예측 모형의 성능개선에 관한 연구이다. 이를 위해 본 연구에서는 단일 모형으로 그 우수성을 인정받고 있는 SVM을 기저 분류기로 사용하는 앙상블 모형에 대해 고찰하였다. SVM 모형의 성능 개선을 위해 bagging과 random subspace 모형을 부도 예측 문제에 적용해 보았으며 bagging 모형과 random subspace 모형의 성과 개선을 위해 bagging과 random subspace의 통합 모형을 제안하였다. 제안한 모형의 성과를 검증하기 위해 실제 기업의 부도 예측 데이터를 사용하여 실험하였고, 실험 결과 본 연구에서 제안한 새로운 형태의 통합 모형이 가장 좋은 성과를 보임을 알 수 있었다.

• 한국항공우주학회지
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• 제31권2호
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• pp.10-16
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• 2003

초음속 유동장에서 비정상 직열배치공동 유동 연구를 Navier-Stokes 방정식과 k-$\varepsilon$난류모델을 이용하여 수치 모사하였다. 공동주위의 비정상유동은 자유 전단층과 공동 내부 유동의 상호 작용에 의한 주기성으로 특정지어진다. 수치적 방법은 van Leer의 유량한계계수를 이용한 유량벡터분리법에 기반을 둔 TVD방법을 사용하였다. 먼저, 단일 공동에 대한 압력비 그래프를 통한 주진동 주파수를 비교하였다. 직열배치공동유동에서는 전후방 공동을 몇가지 세장비로 조합하여, 전방 공동의 영향에 의한 후방 공동 유동장의 주진동 주파수 특성등을 분석하였다. 전방공동의 세장비가 작은 경우에는 강한 전방공동의 전단층이 후방공동의 전단츨과 합하여 하나의 전단층 특성을 줌으로 전후방 공동의 주진동특성이 비슷하게 나타나고, 전방공동의 세장비가 큰경우에는 전방공동의 전단층이 확산되면서 내부에 후방공동의 전단층이 발생함으로 주진동수의 특성이 다르게 나타나는 것을 볼 수 있었다.

• KSBB Journal
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• 제9권1호
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• pp.16-25
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• 1994

Bacillus subtilis를 재조합 이종 단백질 생산 균주로 만들기 위하여 포자형성 변이주를 만들었다. 균주는 두 개의 프로테아제가 제거된 균주인 DB104로부터 spoOJ와 spoIIG 변이주를 유전자 조작법에 의해 만들고 두 개의 유전자가 모두 제거된 균주도 만들었다. 이에 목적 aprE 유전자를 삽입 벡터 형태로 만들어 integration시킨 뒤 변이주 각각의 형태적인 변화를 투과성 전자현미경으로 살펴 보았다. 각각 변이주의 모습은 이전에 보고된 것과 거의 일치하였으며 spoOJ spoIIG 이중포자변이주의 경우는 spoIIG 변이주와 더욱 닮은 것을 알 수 있었으며, 훨씬 주름진 것과 같은 투박한 세포벽 및 막을 가지고 있음을 관찰하였다. spoOJ 변이는 포자형성 빈도를 낮추고 aprE 활성을 감소시키는 반면, spoIIG 변이는 포자형성을 거의 하지 않으면서 aprE 활성에 상승효과를 가져왔다. spoOJ와 spoIIG 이중포자변이주는 spoOJ 변이의 효과는 거의 나타나지 않은채, spoIIG와 비슷한 aprE 활성을 보였다.