• 한국전산유체공학회:학술대회논문집
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• 1995.10a
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• pp.113-117
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• 1995

Viscous analysis on incompressible flows is performed using unstructured triangular meshes. A two-dimensional and axisymmetric incompressible Navier-Stokes equations are solved in time-marching form by artificial compressibility method. The governing equations are discretized by a cell-centered based finite-volume method. and a centered scheme is used for inviscid and viscous fluxes with fourth order artificial dissipation. An explicit multi-stage Runge-Kutta method is used for the time integration with local time stepping and implicit residual smoothing. Convergence properties are examined and solution accuracies are also validated with benchmark solution and experiment.

• 연구논문집
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• s.26
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• pp.57-68
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• 1996

Recently, a lot of researches on material handling system are actively studied for realization of FA(Factory Automation), FMS(Flexible Manufacturing System) and CIM(Computer Integrated Manufacturing). Management and application of material handling system are precondition technologies playing an important part in integration of production system including manufacturing. assemhly, and inspection cell. In this paper, the general situation of automation technology is investigated and technology level is analyzed in comparision with an advanced country. And the operation trend and part of material handling system including AS/RS, AGV, and Conveyor, are studied. And also technologies related to material handling system in the inside and outside of the country are studied.

• Journal of Advanced Marine Engineering and Technology
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• v.29 no.3
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• pp.319-324
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• 2005

This paper reports the performance of Wind-PV(Photovoltaic) hybrid system. The output power of PV is affected by the environmental factors such as solar radiation and cell temperature. Also, the output power of wind system is generated with wind power. Integration of Wind and PV resources, which are generally complementary, usually reduce the capacity of the battery. This paper includes discussion on system reliability, power quality and effects of the randomness of the wind and the solar radiation on system design.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2011.06a
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• pp.727-728
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• 2011

• Biotechnology and Bioprocess Engineering:BBE
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• v.5 no.2
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• pp.84-91
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• 2000

Mycopasma contamination of tissue culture cells easily evades detection and, thus, represents a continous therat to cell biologists. In case where infected cell can not simply be replaced, attempts have to be made to eradicate mycoplacma from the tissue culture cells. A variety of anti-microbial agents have been shown to be toxic to mycoplasma strains ; however, cell associated mycoplasma are often protected from antibiotics at concentrations shown to be effective in vitro. Antibiotic concentrations high enough to be lethal to cell as sociated mycoplasmas frequently are also detrimentrations to the host cells, while moderately increased antibiotic levels tolerated by the host cells often lead to only temporary growth suppression and/or to the emergence of mycoplasma strains resistanct even to high concentrations of the antibiotis applied. Hare, a genetic approach for the elimination of mycoplasma from tissue culture cells that overcomes thens limitations is described. By expression of a selection marker conferring resistance to an otherwise toxic agent, Acholeplasma laidlawii infected BHK-21 cells used as the model system were enabled to temporarily tolerate antibiotic concentrations high enough to be lethal to cell associated mycopalsma while leaving the host cells unharmed. Upon successful mycoplasma eradicated, cultvation of the cured host cells in the absence of the selective agent yielded revertant cell clones that had regained susceptibillity to the toxic agent. Cressation of the selection marker expression was shown to result from the loss of the selection marker DNA, which is a consequence of the fact that the stable and permanent integration of foreign DNA in eucaryotic cell chrosomes is highly inefficient. Thus, the cells were cured from mycoplasma yet remained biochemically unaltered.

• Journal of the Institute of Electronics Engineers of Korea SD
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• v.45 no.8
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• pp.1-10
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• 2008

In this paper, we describe a design of a 128bit MRAM based on a new switching architecture which is Local Field Switching(LFS). LFS uses a local magnetic field generated by the current flowing through an MTJ. This mode reduces the writing current since small current can induce large magnetic field because of close distance between MTJ and the current. It also improves the cell selectivity over using conventional MTJ architecture because it doesn't need a digit line for writing. The MRAM has 1-Transistor 1-Magnetic Tunnel Junction (IT-1MTJ) memory cell structure and uses a bidirectional write driver, a mid-point reference cell block and a current mode sense amplifier. CMOS emulation cell is adopted as an LFS-MTJ cell to verify the operation of the circuit without the MTJ process. The memory circuit is fabricated using a $0.18{\mu}m$ CMOS technology with six layers o) metal and tested on custom board.

• Journal of Hydrogen and New Energy
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• v.14 no.1
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• pp.35-45
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• 2003

Polymer electrolyte fuel cells(PEFC) have been considered to be a suitable candidate for residential, portable and mobile applications, due to their high efficiency and power density, even at low operating temperature. KIER developed a 5kW class PEFC system for residential application and operated the system for over 1,000 hours. To develop a 5kW PEFC system, performance of a cell was improved through successive tests of single cell of small and large area. Fabrication of three 2,5 kW class stacks, design and fabrication of natural gas reformer, design of auxiliary equipments such as DC/DC converter, DC/AC inverter and humidifying units were carried out along with integration of components, operation and evaluation of total system. During the development period from 1999 to 2001, MEA(membrane electrode assembly) fabrication technologies, design and fabrication technologies for separators, stacking technologies and so on were developed, thereby providing basis for developing stacks of higher efficiency and power density in the future. Experience of development of natural gas reformer opened possibilities to use various kinds of fuels. Main results obtained from the development of a 5kW class PEFC system for residential application are summarized.

• Journal of the Korean Society of Manufacturing Technology Engineers
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• v.22 no.2
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• pp.281-286
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• 2013

As the product lifecycle is getting shorter and various models should be released to respond to the needs of customers and markets, automation-based flexible production line has been recognized as the core competitiveness. According to these trends, system vendors supply cell-level systems such as FMC(Flexible Manufacturing Cell) that is integration of core functions of FMS(Flexible Manufacturing System) and RMC(Reconfigurable Manufacturing Cell) that can easily extend components of FMC. In the cell-based environment, flexible management for shop floor composed of existing job shop, FMCs and RMCs from various system vendors has emerged as an important issue. However, there could be some problems on integrated operation between heterogeneous cells to use vendor-specific cell controllers and on seamless information flow with high level systems such as ERP(Enterprise Resource Planning). In this context, this paper proposes process management systems supporting integrated shop operation of heterogeneous multi-cell based flexible manufacturing environment: First of all, (1) Integrated Shop Operation System to apply the process management system is introduced, and (2) Multi-Layer BOP(Bill-Of-Process) model, a backbone of the process management system, is derived with its data structure. Finally, application of the proposed model is illustrated through system implementation results.

• Clinical and Experimental Reproductive Medicine
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• v.41 no.1
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• pp.1-8
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• 2014

Objective: Estrogen related receptor ${\beta}$ (Esrrb) is a member of the orphan nuclear receptors and may regulate the expression of pluripotencyrelated genes, such as Oct4 and Nanog. Therefore, in the present study, we have developed a method for delivering exogenous ESRRB recombinant protein into embryos by using cell-penetrating peptide (CPP) conjugation and have analyzed their effect on embryonic development. Methods: Mouse oocytes and embryos were obtained from superovulated mice. The expression of Oct4 mRNA and the cell number of inner cell mass (ICM) in the in vitro-derived and in vivo-derived blastocysts were first analyzed by real time-reverse transcription-polymerase chain reaction and differential staining. Then 8-cell embryos were cultured in KSOM media with or without $2{\mu}g/mL$ CPP-ESRRB protein for 24 to 48 hours, followed by checking their integration into embryos during in vitro culture by Western blot and immunocytochemistry. Results: Expression of Oct4 and the cell number of ICM were lower in the in vitro-derived blastocysts than in the in vivo-derived ones (p<0.05). In the blastocysts derived from the CPP-ESRRB-treated group, expression of Oct4 was greater than in the non-treated groups (p<0.05). Although no difference in embryonic development was observed between the treated and non-treated groups, the cell number of ICM was greater in the CPP-ESRRB-treated group. Conclusion: Treatment of CPP-ESRRB during cultivation could increase embryos' expression of Oct4 and the formation rate of the ICM in the blastocyst. Additionally, an exogenous delivery system of CPP-conjugated protein would be a useful tool for improving embryo culture systems.

• Communications for Statistical Applications and Methods
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• v.19 no.3
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• pp.495-500
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• 2012

We consider a Bayesian test of independence in a two-way contingency table that has some zero cells. To do this, we take a three-stage hierarchical Bayesian model under each hypothesis. For prior, we use Dirichlet density to model the marginal cell and each cell probabilities. Our method does not require complicated computation such as a Metropolis-Hastings algorithm to draw samples from each posterior density of parameters. We draw samples using a Gibbs sampler with a grid method. For complicated posterior formulas, we apply the Monte-Carlo integration and the sampling important resampling algorithm. We compare the values of the Bayes factor with the results of a chi-square test and the likelihood ratio test.