• Korean Journal of Microbiology
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• v.44 no.3
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• pp.264-269
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• 2008

To develop an amylolytic industrial yeast strain producing $\beta$-amylase, the BAMY gene encoding Achlya bisexualis $\beta$-amylase was constitutively expressed under the control of the alcohol dehydrogenase gene promoter (ADC1p) in an industrial strain of Saccharomyces cerevisiae. Yeast transformation was carried out by an integration system containing $\delta$-sequences as the recombination site. The integrative cassette devoid of bacterial DNA sequences was constructed that contains the BAMY gene and $\delta$-sequences. Industrial S. cerevisiae transformed with this integrative cassette secreted 45 kDa $\beta$-amylase into the culture medium. The $\beta$-amylase activity of the transformant was approximately 18.5-times higher than that of A. bisexualis. The multi-integrated BAMY genes in the transform ant were stable after 100 generations of growth in nonselective medium. Hydrolysis of soluble starch and various starches with the enzyme released maltose but not glucose or oligosaccharides.

• Journal of the Korea institute for structural maintenance and inspection
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• v.16 no.5
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• pp.129-136
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• 2012

Temperature of fresh concrete can be effectively used to predict the strength of concrete being cured and make an informed decision for stripping the molds. A hygrothermograph and thermo-couple sensors that require an extensive wiring have been applied to measure a temperature of concrete at the early stage of the curing process on site. However, these methods have limits to provide the temperature data in real time due to harsh working environment including frequent cutting of wires. Therefore, this study is aiming at developing a device based on wireless sensor network to measure the temperature of concrete being cured in formwork. The result showed that the wireless sensor with probe type thermistor which is developed had the same temperature data compared to the existed wire type thermistor, and we confirmed the temperature history of concrete in real time for 28 days throughout the gateway by wireless network that collects the temperature data measured from specimens in laboratory. Also, the network device for transmission can be easily separated from the probe sensor part and reused consistently. If the wireless sensor network device developed uses in the field, the temperature management of concrete will be systematically conducted from at the early stage of the curing, and especially be effective for cold weather concrete construction. In addition, it will contribute to the establishment of advanced quality control system for concrete and productivity of supervisors on site will be increased in the future.

• Journal of Korea Water Resources Association
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• v.31 no.6
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• pp.821-832
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• 1998

This study investigates the prediction of daily water supply, which is a necessary for the efficient management of water distribution system. Fuzzy neuron, namely artificial intelligence, is a neural network into which fuzzy information is inputted and then processed. In this study, daily water supply was predicted through an adaptive learning method by which a membership function and fuzzy rules were adapted for daily water supply prediction. This study was investigated methods for predicting water supply based on data about the amount of water supplied to the city of Kwangju. For variables choice, four analyses of input data were conducted: correlation analysis, autocorrelation analysis, partial autocorrelation analysis, and cross-correlation analysis. Input variables were (a) the amount of water supplied (b) the mean temperature, and (c)the population of the area supplied with water. Variables were combined in an integrated model. Data of the amount of daily water supply only was modelled and its validity was verified in the case that the meteorological office of weather forecast is not always reliable. Proposed models include accidental cases such as a suspension of water supply. The maximum error rate between the estimation of the model and the actual measurement was 18.35% and the average error was lower than 2.36%. The model is expected to be a real-time estimation of the operational control of water works and water/drain pipes.

• Journal of the Korean Society for Railway
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• v.19 no.1
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• pp.38-45
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• 2016

The power transformation system of High Speed rolling stocks like KTX-Sancheon has shown excellent control capacities in the areas of riding comfortability, switching efficiency, safety and energy consumption due to technical developments in power-electronics, high speed & large scale integrated semiconductors and microprocessors. However, harmonics from IGBT, a high speed switching device used in the Convertor & Invertor equipment of rolling stocks have given rise to various problems in transformer substations, signaling systems, data transmission systems and facility monitoring systems. Especially, TI21 non-insulated track circuits have malfunctioned due to the influence of returning current harmonics which were generated at around of integer times of the number of power transformation equipment in the frequency domain. This paper, measures and analyzes various schemes to analyze the traveling path of the returning current harmonics generated due to the relationship between the rolling stocks and track circuits on site. Ultimately, theseschemes will be used to design high speed rolling stocks, AF track circuits and a common grounding network.

• Journal of the Economic Geographical Society of Korea
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• v.19 no.2
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• pp.399-416
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• 2016

The main aim of the research attempts to identify value relations appropriated and realized in the coffee frontier of Vietnam by investigating the ways in which it is integrated into coffee global value chains driven by multinational companies, and to provide some implications of the integration of the frontier into sustainable coffee global value chains for creating shared value in Dak Lak, Vietnam. Recently Dak Lak has gone through the transition of value relations from exploitative value chains based upon conventional coffee production into shared value chains relied upon the production of sustainable or certified coffee in Dak Lak. The transition has been expected to result in sustainability in the creation of value by enhancing regional competitive advantages and regional bargaining power in global value chains driven by multinational companies. However, the reality has shown the intensification of hierarchical profits allocation among stakeholders such as farmer, middlemen, and multinational companies in the region. The main reasons for this could be found in two perspectives. Firstly, the formation of exclusive relations among farmers, middlemen, and processors has led to stakeholders to secure market, but resulted in the intensification of hierarchy among them in global value chain, because multinational companies could control indirectly over the farming system through exclusive middlemen. Secondly, social and ecological costs imputed by multinational companies to coffee farmers in the name of creating shared value has deteriorated the economic profits of stakeholders such as farmers and middlemen. As a result, it has led to the configuration of systematically hierarchical and subordinated global value chain in Dak Lak.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.12 no.2
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• pp.985-992
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• 2011

The 3D computational fluid dynamics (CFD) was performed in relation to the internal fluid characteristics and flow distribution for the development of the most optimal model in the complex post-disposal device. As it is expected that a channeling (drift) would be made by the semi-dry reactor due to the large difference in the flow distribution by the compartment in the bag filter, a structural improvement should be urgently made for more uniformed flow distribution in the bag filter. Three types of modifications such as i) changing the plenum shape, ii) orifice install in the exit part of cleaned gas, iii) increasing the plenum number were established. From the results of computational fluid dynamics, it was revealed that the changing of plenum shape and orifice install in the exit part of cleaned gas was more reasonable than the increasing the plenum number because of the difficulties of retrofit. The complex post-disposal device, modified and supplemented with this analysis, integrated the semi-dry reactor and the bag filter in a single body, so it follows that the improvement can make the device compact, save the installation area, save the operation fee, and management more convenient.

• The KIPS Transactions:PartC
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• v.16C no.2
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• pp.151-164
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• 2009

The importance of sensor networks as a base of ubiquitous computing realization is being highlighted, and espicially the security is recognized as an important research isuue, because of their characteristics.Several efforts are underway to provide security services in sensor networks, but most of them are preventive approaches based on cryptography. However, sensor nodes are extremely vulnerable to capture or key compromise. To ensure the security of the network, it is critical to develop security Intrusion Detection System (IDS) that can survive malicious attacks from "insiders" who have access to keying materials or the full control of some nodes, taking their charateristics into consideration. In this perper, we design a distributed and adaptive IDS architecture on sensor networks, respecting both of energy efficiency and IDS efficiency. Utilizing a modified HEED algorithm, a clustering algorithm, distributed IDS nodes (dIDS) are selected according to node's residual energy and degree. Then the monitoring results of dIDSswith detection codes are transferred to dIDSs in next round, in order to perform consecutive and integrated IDS process and urgent report are sent through high priority messages. With the simulation we show that the superiorities of our architecture in the the efficiency, overhead, and detection capability view, in comparison with a recent existent research, adaptive IDS.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.11 no.11
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• pp.4656-4663
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• 2010

In this study, the 3D computational fluid dynamics (CFD) was performed in relation to the internal fluid characteristics, flow distribution, air mean ages, and residence time for the development of the most optimal model in the complex post-disposal device. As it is expected that a channeling (drift) would be made by the semi-dry reactor due to the large difference in the flow distribution by the compartment in the bag filter, a structural improvement should be urgently made for more uniformed flow distribution in the bag filter. In addition, it showed the possibility that the velocity field and distribution characteristics of the residence time could be improved through a modification to inlet structure of the spray dryer reactor. The complex post-disposal device, modified and supplemented with this analysis, integrated the semi-dry reactor and the bag filter in a single body, so it follows that the improvement can make the device compact, the installation area, the operation fee, and management more convenient.

• Journal of Biomedical Engineering Research
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• v.40 no.5
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• pp.197-205
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• 2019

The existing Extracorporeal membrane oxygenation(ECMO) and Cardiopulmonary bypass system(CPB) have been developed and applied to various devices according to their respective indications. However, due to the complicated configuration and difficult usage method, it causes inconvenience to users and there is a risk of an accident. Therefore, smart all-in-one cardiopulmonary circulation device is being developed recently. The smart all-in-one cardiopulmonary assist device consists of a blood pump for cardiopulmonary bypass, a blood oxidizer for cardiopulmonary bypass, a blood circuit for cardiopulmonary bypass, and an artificial cardiopulmonary device. It is an integrated cardiopulmonary bypass device that can be used for a variety of purposes such as emergency, intraoperative, post-operative intensive care, and long-term cardiopulmonary assist, combined with CPB used in open heart surgery and ECMO used when patient's cardiopulmonary function does not work normally. The smart all-in-one cardiopulmonary assist device does not exist as a standard and international standard applicable to advanced medical devices. Therefore, in this study, we will refer to the International Standard for Blood Components, the International Standard for Blood, the Guideline for Blood Products, and prepare applicable performance and safety guidelines to help quality control of medical devices, and contribute to the improvement of the health of people. The guideline, which is the result of conducted a survey of the method of safety and performance test, is based on the principle of all-in-one cardiopulmonary aiding device, related domestic foreign standards, the status of domestic and foreign patents, related literature, blood pump(ISO 18242), blood oxygenator (ISO 7199), and blood circuit (ISO 15676) for cardiopulmonary bypass.The items on blood safety are as follows: American Society for Testing and Materials ASTM F1841-97R17), and in the 2010 Food and Drug Administration's Safety Assessment Guidelines for Medical Assisted Circulatory Devices. In addition, after reviewing the guidelines drawn up through expert consultation bodies including manufacturers / importers, testing inspectors, academia, etc. the final guideline was established through revision and supplementation process. Therefore, we propose guidelines for evaluating the safety and performance of smart all-in-one cardiopulmonary assist devices in line with growing technology.

• The Korean Journal of Food And Nutrition
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• v.34 no.5
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• pp.477-486
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• 2021

To utilize Malus pruniforia Borkh. as a functional material, cold-water (CW), hot-water (HW), and 70% ethanol (EtOH) extracts were prepared, and their antioxidant and anti-inflammatory activities were compared. The antioxidant activity of the HW extract evaluated by ABTS and DPPH radical scavenging and FRAP activity was significantly effective. The total polyphenol content of the HW extract was also higher by 15.5±0.7 mg GAE/g extract compared to other extracts. The EtOH extract showed significantly decreased TNF-α (39.8%), IL-6 (65.5%), and NO (34.9%) levels in RAW 264.7 cells compared to the LPS-induced control group. The levels of IL-6 (21.1%) and IL-8 (19.3%) were significantly decreased by treatment of EtOH extract in HaCaT keratinocytes induced with TNF-α and IFN-γ. The UHPLC-MS results indicated that the EtOH extract might have chlorogenic acid and phlorizin as the major compounds. This was validated using HPLC-DAD, which showed that the EtOH extract had higher levels of chlorogenic acid and phlorizin (1,185±58 and 470±10 ㎍/g extract, respectively). In conclusion, the present study suggested that the anti-inflammatory activity of the EtOH extract was more effective than the CW and HW extracts, and chlorogenic acid and phlorizin could be used as indicator compounds and functional substances.