• Journal of Microbiology and Biotechnology
• /
• 제17권2호
• /
• pp.234-243
• /
• 2007

Vibrio vulnificus is an opportunistic pathogen that causes septicemia in humans. To identify the genes associated with its pathogenicity, in vivo expression technology (IVET) was used to select genes specifically expressed in a host, yet not significantly in vitro. Random lacZ-fusions in the genome of V vulnificus strain MO6-24/O were constructed using an IVET vector, pSG3, which is a suicide vector containing promoterless-aph and -lacZ as reporter genes. A total of ${\sim}18,000$ resulting library clones were then intraperitoneally injected into BALB/c mice using a colony forming unit (CFU) of $1.6{\times}10^6$. Two hours after infection, kanamycin was administered at $200{mu}g$ per gram of mouse weight. After two selection cycles, 11 genes were eventually isolated, which were expressed only in the host. Among these genes, VV20781 and VV21007 exhibiting a homology to a hemagglutinin gene and tolC, respectively, were selected based on having the highest frequency. When compared to wild-type cells, mutants with lesions in these genes showed no difference in the rate of growth rate, yet a significant decrease in cytotoxicity and the capability to form a biofilm.

• 문화기술의 융합
• /
• 제9권4호
• /
• pp.23-30
• /
• 2023

본 연구는 간호대학생의 통합임상실습 전·후 학습성과의 차이를 파악하여 효율적인 임상실습 교육 운영을 위한 기초자료로 활용하고자 시도되었다. 단일군 사전·사후 설계로 자료수집은 2021년 7월 19일부터 9월 10일까지 간호대학생 38명을 대상으로 2주간 통합임상실습 전·후 이루어졌다. 수집된 자료는 SPSS/Win 23.0을 이용하여 빈도, Wilcoxon sign rank, 피어슨 상관관계로 분석하였다. 통합임상실습 후 제1학습성과(통합적용)(Z=-4.63, p<.001), 제2학습성과(핵심수행)(Z=-3.99, p<.001), 제6학습성과(비판적사고)(Z=-3.60, p<.001) 및 제11학습성과(연구수행)(Z=-2.76, p=.005)가 실습 전보다 유의하게 증가하였다. 통합임상실습이 학습성과를 향상시키는 효과적인 실습인 것으로 나타났으므로 학습성과 향상을 위한 전략으로 적극 활용하기를 제언한다.

• 전기화학회지
• /
• 제11권3호
• /
• pp.165-169
• /
• 2008

CODH(Carbon Monoxide Dehydrogenase)에 의한 이산화탄소 환원에 있어서 작업전극을 유리탄소전극을 사용한 경우와 금전극을 사용한 경우를 비교하여 그 영향을 관찰하였다. 금전극을 사용한 경우에는 수소발생과 섞이기 때문에 전기분해의 전위를 잘 선택해야 효율적인 이산화탄소의 환원 반응을 관찰할 수 있는데 반하여, 유리탄소전극은 금전극보다 수소 환원에 대한 과전압이 크기 때문에 -650 mV vs. NHE 까지도 중성수용액에서 수소발생 없이, 효율적인 이산화탄소의 환원을 관찰할 수 있었다. CODH를 이용한 이산화탄소의 환원에는 가해주는 전기분해 전위가 큰 영향을 미침을 알 수 있었는데, $-570{\sim}600\;mV$ vs. NHE 근처가 가장 효율적임을 알 수 있었고 이보다 더 음의 전위를 걸어주었을 때는 효소활성의 감소 및 수소발생이 복합적으로 영향을 미쳐 일산화탄소 생성의 전류효율이 급격히 감소함을 알 수 있었다.

• 한국환경교육학회지:환경교육
• /
• 제10권2호
• /
• pp.249-268
• /
• 1997

This Study developed 'Environment Management Integrated Leader(EMIL)' program and evaluated the effect of the Program. The procedure of EMIL program development is as follows; 1) Identifying the training needs Through the interview and discussion with corporate environmental staff and research members, training need are collected. 2) Setting the training objectives 30 objectives are developed through specifying and organizing the training needs. 3) Determining the scopes of the program This program consists of the four scopes. First, the scope of Environmentally Acceptable Management is developed based on ISO 14000 and certification of environmentally friendly business. Second, the scope of Environmentally Acceptable Technology includes air pollution, water pollution, solid wasted management, and pollution prevention. Third, the scope of Integrated Environmental Management focuses on master plan, green marketing, green production, and green accounting. Finally, the scope of Integrated Environmental Concepts focuses on food pollution, environmental activities, and bio-diversity. 4) Selecting the training methods Various methods are used such as off-site visits, exercises, case study, workshop, discussion, role-playing as well as lecture. 5) Implementing the program 49 trainees participated the program for 6weeks. All trainees are working for D. Company Group. 6) Measuring training effects To evaluate the effects of the program, pre-post training evaluation method is used. The results are highly significant at all 4 scopes by the p<.01. This study has some implications in order to establish the environmental management climate. First, the development of new environmental education program for management is necessary. Second, Vast investment are required. Third, more systematic approaches are desired in environmental education. Lastly, more customized program development and implementation to fit in industries will accelerate the expansion of environmental concept.

• KSBB Journal
• /
• 제16권6호
• /
• pp.538-546
• /
• 2001

효모의 세포주기 관련 유전자 발현 통합 데이터를 사용하여 본 연구실에서 개발한 유전자 발현 통합 분석프로그램을 사용하여, 클러스터링 알고리즘의 성능을 비교하고 데이터내에 존재하는 클러스터 개수를 추정하기 위해 FOM 분석을 적용하였으며, 이 분석방법을 통하여 K-means, SOM, Fuzzy c-means 클러스터링 방법의 성능을 서로 비교하였다. 클러스터 개수를 추정한 다음 3가지 클러스터링 방법에 대한 클러스터링 결과 비교, 클러스터의 기능할당 및 모티프 분석을 시도하였다. 본 논문에서 제시하는 분석 방법은 DNA chip 발현 데이터의 일반적인 분석방법을 유전자 발현 패턴의 유사성을 토대로 한 클러스터링 방법에 근간을 두고 있다. 본 논문에서는 클러스터링한 후 각 클러스터의 기능할당 및 모티프 분석에 대한 일반적인 분석방법을 제시하였으며, 본 연구실에서 개발한 유전자 발현분석 통합 프로그램이 효율적으로 사용될 수 있음을 보여주고 있다.

• 제어로봇시스템학회:학술대회논문집
• /
• 제어로봇시스템학회 1997년도 한국자동제어학술회의논문집; 한국전력공사 서울연수원; 17-18 Oct. 1997
• /
• pp.716-719
• /
• 1997

An integrated program of driving simulator has been developed for excavators using the Motif, OpenGL, and C compiler. The developed program not only offers a GUI but also covers graphic algorithms, therefore, the user can easily run the driving simulator whose components include a simplified visual graphics system. Several graphics technique are combined and applied to the simulator program in order to increase the speed of graphical representation, which access computer memories, mix 2D models with 3D ones, and use the basic position detection method. A text format environment file has been utilized for organizing more flexible driving circumstances.

• Nuclear Engineering and Technology
• /
• 제38권7호
• /
• pp.591-618
• /
• 2006

An integrated R&D program is being conducted to study, qualify, and in some cases, develop materials with required properties for the reactor systems being developed as part the U.S. Department of Energy's Generation IV Reactor Program. The goal of the program is to ensure that the materials research and development (R&D) needed to support Gen IV applications will comprise a comprehensive and integrated effort to identify and provide the materials data and its interpretation needed for the design and construction of the selected advanced reactor concepts. The major materials issues for the five primary systems that have been considered within the U.S. Gen IV Reactor Program-very high temperature gas-cooled, supercritical water-cooled, gas-cooled fast spectrum, lead-cooled fast spectrum, and sodium-cooled fast spectrum reactors-are described along with the R&D that has been identified to address them.

• 한국콘텐츠학회논문지
• /
• 제22권9호
• /
• pp.657-666
• /
• 2022

본 연구는 통합치유프로그램이 지적장애인 성폭력 피해자의 자아존중감과 자기표현능력 및 성적 자기결정 능력에 미치는 효과를 검증하기 위하여 실시하였다. 연구대상자는 K지역의 여성장애인 성폭력상담소에 의뢰된 지적장애인 성폭력 피해자 8명이다. 프로그램의 효과성을 검증하기 위해 SPSS Win 25.0 프로그램을 사용하여 사전·사후 검사에 따른 T-test를 실시하였다. 연구결과, 통합치유프로그램은 첫째, 지적장애인 성폭력 피해자의 자아존중감이 통계적으로 유의미(t=-4.183, p<0.001)하게 향상되었다. 둘째, 자기표현능력도 통계적으로 유의미(t=-5.103, p<0.001)하게 개선되었다. 셋째, 성적 자기결정능력에 있어서도 긍정적인 행동의 변화(t=-26.346, p<0.001)를 유도함으로써 통합치유프로그램의 효과성을 관찰할 수 있었다. 이러한 연구결과는 지적장애인 성폭력 피해자의 심리·정서적 치유를 위해 통합치유프로그램을 적극적으로 활용하여야 함을 시사한다.

• 한국해양공학회지
• /
• 제23권4호
• /
• pp.78-84
• /
• 2009

This paper presents development results of integrated operating program and its simulator for an inshore test miner. The inshore test miner drives on the seabed and collects manganese nodules. For operating the test miner, integrated operating program (IOP) is needed for remote control and monitor of the miner in real-time. However, it is hard to develop the practical IOP for the test miner system, because the system is big, expensive, and unique. So a simulator was developed as a test substitute in order to save time and to reduce cost. Using test results of the simulator, the IOP of the test miner was developed. The developed IOP consists of remote program, host program, monitoring program and database program. The operating program was developed using PXI and Labview of NI. The experimental results are presented to show the applicability of the developed program.

• Journal of Electrochemical Science and Technology
• /
• 제12권1호
• /
• pp.137-145
• /
• 2021

Among the non-precious metal catalysts, iron-nitrogen doped carbon (Fe-N/C) catalysts have been recognized as the most promising candidates for an alternative to Pt-based catalysts for the oxygen reduction reaction (ORR) under alkaline and acidic conditions. In this study, the nano replication method using mesoporous silica, which features tunable primary particle sizes and shape, is employed to prepare the mesoporous Fe-N/C catalysts with different shapes. Platelet SBA-15, irregular KIT-6, and spherical silica particle (SSP) were selected as a template to generate three different kinds of shapes of the mesoporous Fe-N/C catalyst. Physicochemical properties of mesoporous Fe-N/C catalysts are characterized by using small-angle X-ray diffraction, nitrogen adsorption-desorption isotherms, and scanning electron microscopy images. According to the electrochemical evaluation, there is no morphological preference of mesoporous Fe-N/C catalysts toward the ORR activity with half-cell configuration under alkaline electrolyte. By implementing X-ray photoelectron spectroscopy analysis of Fe and N atoms in the mesoporous Fe-N/C catalysts, it is possible to verify that the activity towards ORR highly depends on the portions of "Fe-N" species in the catalysts regardless of the shape of catalysts. It was suggested that active site distribution in the Fe-N/C is one important factor towards ORR activity.