• Mass Spectrometry Letters
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• v.6 no.3
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• pp.80-84
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• 2015

Here, we demonstrate the use of MALDI-TOF as a fast and simple analytical approach to evaluate the DNA-binding capability of various peptides. Specifically, by varying the amino acid sequence of the peptides consisting of lysine (K) and tryptophan (W), we identified peptides with strong DNA-binding capabilities using MALDI-TOF. Mass spectrometric analysis reveals an interesting novel finding that lysine residues show sequence selective preference, which used to be considered as mediator of electrostatic interactions with DNA phosphate backbones. Moreover, tryptophan residues show higher affinity to DNA than lysine residues. Since there are numerous possible combinations to make peptide oligomers, it is valuable to introduce a simple and reliable analytical approach in order to quickly identify DNA-binding peptides.

• Journal of KIISE:Computer Systems and Theory
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• v.33 no.10
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• pp.746-758
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• 2006

Many objects existing on wide area environments have the replication characteristics according to how to categorize using their own names or properties. From the clients' requests, the existing naming and trading services have not supported with the binding service for replicated solver object with the same service type. For this reason, we present an integrated model that can support the selection of replicated object and dynamic binding services on wide-area computing environments. This model suggests provides not only location management of replicated objects but also active binding service which enables to select a least-loaded object on the system to keep the balance of load between systems. In this purpose, constructing both the service plan and model for support solver object's binding with replication property on wide area computing environments has been researched. In this paper, we showed the test environment and analyzed the performance evaluation of client/server binding procedures via integrated binding service in federation model and verified our model under the condition to see whether load balance can be applied to our model. For the performance evaluation of suggested wide area integrated binding service federation model, evaluated the integrated binding service of each domain and analyzed the performance evaluation of process for non-replication object's under federation model environment. Also, we analyzed the performance evaluation of the federation model between domains for wide area environment. From the execution results, we showed the federation model provides lowers search-cost on the physical tree structure of network.

• Biomolecules & Therapeutics
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• v.30 no.4
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• pp.380-388
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• 2022

Snail is implicated in tumour growth and metastasis and is up-regulated in various human tumours. Although the role of Snails in epithelial-mesenchymal transition, which is particularly important in cancer metastasis, is well known, how they regulate tumour growth is poorly described. In this study, the possible molecular mechanisms of Snail in tumour growth were explored. Baculoviral inhibitor of apoptosis protein (IAP) repeat-containing protein 3 (BIRC3), a co-activator of cell proliferation during tumourigenesis, was identified as a Snail-binding protein via a yeast two-hybrid system. Since BIRC3 is important for cell survival, the effect of BIRC3 binding partner Snail on cell survival was investigated in ovarian cancer cell lines. Results revealed that Bax expression was activated, while the expression levels of anti-apoptotic proteins were markedly decreased by small interfering RNA (siRNA) specific for Snail (siSnail). siSnail, the binding partner of siBIRC3, activated the tumour suppressor function of p53 by promoting p53 protein stability. Conversely, BIRC3 could interact with Snail, for this reason, the possibility of BIRC3 involvement in EMT was investigated. BIRC3 overexpression resulted in a decreased expression of the epithelial marker and an increased expression of the mesenchymal markers. siSnail or siBIRC3 reduced the mRNA levels of matrix metalloproteinase (MMP)-2 and MMP-9. These results provide evidence that Snail promotes cell proliferation by interacting with BIRC3 and that BIRC3 might be involved in EMT via binding to Snail in ovarian cancer cells. Therefore, our results suggested the novel relevance of BIRC3, the binding partner of Snail, in ovarian cancer development.

• Journal of KIISE:Information Networking
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• v.30 no.3
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• pp.293-306
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• 2003

In recent years, distributed computing environments have been radically changing to a structure of global, heterogeneous, federative and wide-area systems. This structure's environments consist of a let of objects which are implemented on telecommunication network to provide a wide range of services. Furthermore, all of objects existing on the earth have the duplicated characteristics according to how to categorize their own names or properties. But, the existing naming or trading mechanism has not supported the binding services of duplicated objects, because of deficiency of independent location service. Also, if the duplicated objects which is existing on different nodes provide the same service, it is possible to distribute the client requests considering each system's load. For this reason, we designed and implemented a new model that can not only support the location management of replication objects, but also provide the dynamic binding service of objects located in a system with minimum overload for maintaining load balancing among nodes in wide-area object computing environments. Our model is functionally divided into two parts; one part is to obtain an unique object handle of replicated objects with same property as a naming and trading service, and the other is to search one or more contact addresses by a location service using a given object handle. From a given model mentioned above, we present the procedures for the integrated binding mechanism in design phase, that is, Naming/Trading Service and Location Service. And then, we described in details the architecture of components for Integrated Binding Service implemented. Finally, we showed our implement environment and executing result of our model.

• Journal of Microbiology and Biotechnology
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• v.22 no.1
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• pp.46-49
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• 2012

The Vibrio vulnificus vuuA gene, of which expression is repressed by a complex of iron and ferric uptake regulator (Fur), was characterized to localize the Fur-binding site in its upstream regulatory region. In silico analysis suggested the presence of two possible Fur-binding sites; one is a classical Fur-box and the other is a previously reported distinct Fur-binding site. Site-directed mutagenesis and DNase I protection assays revealed the binding site for the iron-Fur complex, which includes an extended inverted repeat containing a homologous sequence to the classical Fur-box.

• Proceedings of the Korea Concrete Institute Conference
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• 2006.11a
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• pp.537-540
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• 2006

Over the past few decades, a considerable number of studies on the durability of concrete have been carried out extensively. A lot of improvements have been achieved especially in modeling of ionic flows. However, the majority of these researches have not dealt with the chloride binding isotherm based on the mechanism, although chloride binding capacity can significantly impact on the total service life of concrete under marine environment. The purpose of this study is to develop the model of chloride binding isotherm based on the individual mechanism. It is well known that chlorides ions in concrete can be present; free chlorides dissolved in the pore solution, chemical bound chlorides reacted with the hydration compounds of cement, and physical bound attracted to the surface of C-S-H grains. First, sub-model for water soluble chloride content is suggested as a function of pore solution and degree of saturation. Second, chemical model is suggested separately to estimate the response of binding capacity due to C-S-H and Friedel's salt. Finally, physical bound chloride content is estimated to consider a surface area of C-S-H nano-grains and the distance limited by the Van der Waals force. The new model of chloride binding isotherm suggested in this study is based on their intrinsic binding mechanisms and hydration reaction of concrete. Accordingly, it is possible to characterize chloride binding isotherm at the arbitrary stage of hydration time and arbitrary location from the surface of concrete. Comparative study with experimental data of published literature is accomplished to validity this model.

• Molecular & Cellular Toxicology
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• v.3 no.sup4
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• pp.38-38
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• 2007

• The Transactions of The Korean Institute of Electrical Engineers
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• v.58 no.1
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• pp.147-154
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• 2009

This paper presents a grating~integrated SPR (Surface Plasmon Resonance) sensor chip for simple and inexpensive biomolecule detection. The grating-integrated SPR sensor chip has two sensing channels having a nano grating for SPR coupling. An external mirror is used for multi channel SPR sensing. The present sensor chip replaces bulky and expensive optical components, such as fiber-optic switches or special shaped prisms, resulting in a simple and inexpensive wavelength modulated multi-channel SPR sensing system. We fabricate a SPR sensor chip integrated with 835 nm-pitch gratings by a micromolding technique to reduce the fabrication cost. In the experimental characterization, the refractive index sensitivity of each sensing channel is measured as $321.8{\pm}8.1nm$/RI and $514.3{\pm}8.lnm$/RI, respectively. 0.5uM of the target biomolecule (streptavidin) was detected by a $1.13{\pm}0.16nm$ shift of the SPR dip in the 10%-biotinylated sample channel, while the SPR dip in the reference channel for environmental perturbation monitoring remained at the same position. From the experimental results, multi-channel biomolecule detection capability of the present grating-integrated SPR sensor chip has been verified. On the basis of the preliminary experiments, we successfully measured the binding reaction rate for the $2\;nM{\sim}200\;nM$ monoclonal-antibiotin, thus verifying biomolecule concentration detectability of the present SPR sensor chip. The binding reaction rates measured from the present SPR sensor chip agredd well with those from a commercialized SPR sensor.

• The KIPS Transactions:PartA
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• v.9A no.2
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• pp.241-248
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• 2002

With the structure of tilde-area computing system which Is specified by a researching team in Vrije University, Netherlands, lots of researchers and developers have been progressing the studies of global location and interconnection services of distributed objects existing in global sites. Most of them halve focused on binding services of only non-duplicated computational objects existing wide-area computing sites without any consideration of duplication problems. But all of objects existing on the earth rave the duplicated characteristics according to how to categorize their own names or properties. These objects with the same property can define as duplicated computational objects. Up to now, the existing naming or trading mechanism has not supported the binding services of duplicated objects, because of deficiency of independent location service. For this reason, we suggest a new model that can not only manages locations of duplicated objects In wide-area computing environments, but also provide minimum binding time by considering both the optimal selection of one of duplicated objects and load balance among distributed systems. Our model is functionally divided into 2 parts, one part to obtain an unique object handle of duplicated objects with same property as a naming and trading service, and the other to search one or more contact addresses by a node manager using a liven object handle, as a location service For location transparency, these services are independently executing each other. Based on our model, we described structure of wide-area integrated tree and algorithms for searching and updating contact address of distributed object on this tree. finally, we showed a federation structure that can globally bind distributed objects located on different regions from an arbitrary client object.

• Journal of Korean Society of Water and Wastewater
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• v.20 no.6
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• pp.893-904
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• 2006

To improve sensitivity of biosensor as yeast two-hybrid detection system for estrogenic activity of suspected chemicals, we tested effects of several combinations of the bait and fish components in the two-hybrid system on Saccharomyces cerevisiae inducted a chromosome-integrated lacZ reporter gene that was under the control of CYC1 promoter and the upstream Gal4p-binding element $UAS_{GAL}$. The bait components that were fused with the Gal4p DNA binding domain are full-length human estrogen receptor ${\alpha}$ and its ligand-binding domain. The fish components that were fused with the Gal4p transcriptional activation domain were nuclear receptor-binding domains of co-activators SRC1 and TIF2. We found that the combination of the full-length human estrogen receptor ${\alpha}$ with the nuclear receptor-binding domain of co-activator SRC1 was most effective for the estrogen-dependent induction of reporter activity among the two-hybrid systems so far reported. The relative strength of transcriptional activation by representative natural and xenobiotic chemicals was well correlated with their estrogenic potency that had been reported with other assay systems.