• Title/Summary/Keyword: Intact

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EFFECT OF HOT SEASON ON LIVE SPERMATOZOA WITH INTACT ACROSOME IN HOLSTEIN BULLS

  • Terawaki, Y.;Fujita, H.;Fukui, Y.
    • Asian-Australasian Journal of Animal Sciences
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    • v.8 no.5
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    • pp.415-418
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    • 1995
  • Semen characteristics were examined to find the deterioration of the percentage of live spermatozoa with intact acrosome during hot season using 5 Holstein bulls located in Shirnizu-cho Hokkaido Japan. Spermatozoal viability and acrosomal status were observed simultaneously with triple-stain technique for each spermatozoon. Spermatozoa were divided in four categories (live spermatozoa with intact acrosome, live spermatozoa without intact acrosome, dead spermatozoa with intact acrosome and dead spermatozoa without intact acrosome). Bull and collection month had significant effects on semen characteristics (p < 0.01). The percentage of live spermatozoa with intact acrosome and the percentage of live spermatozoa had the lowest least squares mean by collection month in August (72.7% and 76.7%). These two characteristics indicated the obvious deterioration during hot season. But the fluctuation of these two characteristics were not parallel and the differences between the two characteristics were largest during July to September. The present results indicate the necessity for the simultaneous determination of viability and acrosomal status of each Holstein bull's spermatozoa in order to keep fertility above an acceptable minimum level during hot season.

Scavenge of superoxide and hydrogen peroxide by bovine intact red blood cells (한우 무손상 적혈구의 superoxide 및 과산화수소 제거능력)

  • Cho, Jong-hoo;Park, Sang-youel
    • Korean Journal of Veterinary Research
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    • v.38 no.2
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    • pp.273-279
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    • 1998
  • The ability of bovine intact red blood cells to scavenge superoxide and hydrogen peroxide by superoxide dismutase, catalase and glutathione peroxidase was investigated. Intact red cells(up to 0.4%) suspensions did not inhibit ferricytochrome c reduction by superoxide in the superoxide generating system. On the other hand, intact red cell(0.4%) suspensions almost completely inhibit ferrocytochrome c oxidation by hydrogen peroxide. The ability of intact red cells to scavenge hydrogen peroxide was mainly attributed to either membrane bound catalase or glutathione peroxidase. The scavenge of hydrogen peroxide by 0.1~0.2% intact red cells showed a trend of dependence on mainly glutathione peroxidase. However, at blood cell concentration higher than 0.3%, the process depended upon peroxidase-independent scavengers like catalase. Enhancement of ferrocytochrome c oxidation by red cells treated with aminotriazole proved that the protection against hydrogen peroxide was due to catalase, while the protection in the presence of glutathione indicated scavenging effect of glutathione peroxidase against hydrogen peroxide.

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Maternal Parenting Behaviors Perceived by Children and Mothers' Family Stress in Low Income Divorced and Intact Families (저소득층 아동이 지각하는 어머니 양육 행동과 어머니가 지각하는 가족 스트레스: 이혼 가정과 양부모 가정 중심으로)

  • Han, Jun-Ah
    • Journal of the Korean Home Economics Association
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    • v.48 no.8
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    • pp.87-97
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    • 2010
  • The purposes of this study were to explore the differences in maternal parenting behaviors and mothers' family stress by children's sex and parental divorce and to investigate the relationships of maternal parenting behaviors with mothers' family stress in low income families. Participants of this study were 166 children of 4 to 6 grades and their mothers from low income divorced and intact families. The data were analyzed by descriptive statistics, two-way ANOVAs, Pearson's correlations. The result were as follows: Girls perceived more maternal warmth than boys in divorce families, but boys perceived more maternal warmth than girls in intact families. Boys perceived more maternal supervision than girls in intact and divorced families. Mother of divorced families had more childrearing stress than mothers of intact familes. In divorced families, children whose mothers had high levels of childrearing stress perceived less maternal warmth and supervision, and children whose mothers had high levels of work-family conflicts perceived less maternal warmth. In intact families, children whose mothers had high levels of economic stress perceived less maternal supervision.

The Possible Participation of the Mesophyll on Stomatal Opening

  • Lee, Joon-Sang
    • Korean Journal of Environmental Biology
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    • v.19 no.1
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    • pp.1-6
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    • 2001
  • Many researchers have been studied with guard cell protoplasts and detached epidermis as they think that properly stabilized protoplasts and detached epidermis retain many of the properties of intact guard cells. However, some studies have shown that stomata in detached epidermis behave differently, both quantitatively and qualitatively, from those in the intact leaf. Stomata in the intact leaf are very sensitive to environmental factors such as light, $CO_2$ and osmotic stress, but stomata in detached epidermis are less sensitive to these factors than those in the intact leaf. The clearest evidence to suggest the different response between detached epidermis and intact leaf obtained from the experiments with heavy metal, cadmium. 3-weeks old Commelina. communis was transferred to and grown in Hoagland solution in the presence or absence of 5 mM $Cd^{2+}$ for 4 days. The application of $Cd^{2+}$ showed about 70% inhibition of stomatal conductance when measured at various light intensity (100-1,000 $\mu$mole $m^{-2}s^{-1}). However, stomata in detached epidermis floated on an incubation medium containing 100 $\mu$M $Cd^{2+}$ opened to a degree of about 8.38 fm, but the stomata treated with no cadmium opened to 3.74 ${\mu}{\textrm}{m}$. These results were unexpected as the intact leaf grown in a Hoagland solution containing cadmium showed very negative physiological responses. These results showed that stomata in detached epidermis and in the intact leaf could respond reversely. Therefore, it is possible that we now misunderstand how stomata open in real natural condition.

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Semi-supervised Multi-view Manifold Discriminant Intact Space Learning

  • Han, Lu;Wu, Fei;Jing, Xiao-Yuan
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.12 no.9
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    • pp.4317-4335
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    • 2018
  • Semi-supervised multi-view latent space learning is gaining considerable popularity recently in many machine learning applications due to the high cost and difficulty to obtain the large amount of label information of data. Although some semi-supervised multi-view latent space learning methods have been presented, there is still much space for improvement: 1) How to learn latent discriminant intact feature representations by employing data of multiple views; 2) How to exploit the manifold structure of both labeled and unlabeled point in the learned latent intact space effectively. To address the above issues, we propose an approach called semi-supervised multi-view manifold discriminant intact space learning ($SM^2DIS$) for image classification in this paper. $SM^2DIS$ aims to seek a manifold discriminant intact space for data of different views by making use of both the discriminant information of labeled data and the manifold structure of both labeled and unlabeled data. Experimental results on MNIST, COIL-20, Multi-PIE, and Caltech-101 databases demonstrate the effectiveness and robustness of our proposed approach.

Ptotoplast Formation and Comparison of Phosphatase Activity between Intact Cell and Protoplast in Sccharomyces uvarum. (Sccharomyces uvarum의 protoplast 형성 및 intact cell과 protoplast의 phosphatase 활성도 비교)

  • Lee, Ki-Sung;Kim, Young-Ho
    • The Journal of Natural Sciences
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    • v.11 no.1
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    • pp.55-63
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    • 1999
  • In this study, the stabilizer, PH and lysis method for optimum condition of S. uvarum protoplast formation were investigated, and also enzyme activity and poly-P formation of intact cell and protoplast mere compared. Upon protoplast formation, incubation time of 5 hours in snail gut enzyme and 3 hours in drisielase were reignited. 0.8 Mole mannitol and 6 mole KCl were apt to protoplast formation. Protoplast was contained less 22-27 percentage in ALPase, 4-15 percentage in ACPase than intact cell. Accumulation of inorganic polyphosphate did not increase significently in protoplast compared with intact cell.

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Enhancement of Fertilizing Ability of Frozen-Thawed Bovine and Human Spermatozoa Treated with Fertilizing Promoting Peptide or Pentoxifylline (Fertilizing Promoting Peptide와 Pentoxifylline으로 처리된 소와 사람 동결 정액의 수정능 향상)

  • Lee, K.S.;Kim, E.Y.;Park, S.Y.;Shin, H.A.;Park, S.P.;Lim, J.H.;Chung, K.S.;Lee, H.T.
    • Korean Journal of Animal Reproduction
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    • v.25 no.4
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    • pp.409-419
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    • 2001
  • This study was to examine whether the in vitro friability, motility and intact acrosome of frozen-thawed bovine and human sperm can be improved by adding Pentoxifylline (PF) or Fertilization Promoting Peptide (FPP). Human semen was frozen ultra-rapidly using Test yolk-buffer (TYB) freezing medium. Additive (PF, FPP) effects in frozen-thawed bovine and human sperm were analyzed by microscopic count for sperm motility and coomassie brilliant blue staining method f3r sperm acrosome intact. The in vitro motility of frozen-thawed bovine sperm with 5 mM PF treatment group (50.0%) was significantly higher than that of control (34.0%) (P<0.05). In the frozen-thawed bovine sperm was examined, the intact acrosome rate of 50 nM FPP treatment (49.0%) was significantly higher than those of control (30.0%) and 25 nM FPP (38.0%) treatment groups (P<0.01). In human semen, when in vitro motility of sperm with PF addition prior to freezing was examined, the result of 5 mM treatment group (51.0%) was significantly higher than those of control and 2.5 mM treatment group (39.0, 40.0%) (P<0.01). In addition, 50 nM (75.5%) FPP adding in all treatment procedures for human semen freezing (before freezing, freezing and after thawing) was significant effect on maintenance of the sperm intact acrosome percentage (control: 45.0; 25 nM: 53.0; 100 nM: 68.0%) (P<0.01). Also, the intact acrosome rate of human sperm with FPP (65.0%) was significantly higher than that with PF (43.0%) (P<0.05), although sperm motility was slightly higher in PF treatment group. These results suggest that improved sperm motility and intact acrosome of frozen thawed bovine and human sperm can be obtained by addition of PF or FPP, and that the enhanced in vitro viability, motility and intact acrosome can be obtained by addition of FPP in all semen freezing procedures.

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Comparison of Some Selected Growth, Physiological and Bone Characteristics of Capon, Slip and Intact Birds in Taiwan Country Chicken Cockerels

  • Lin, Cheng-Yung;Hsu, Jenn-Chung
    • Asian-Australasian Journal of Animal Sciences
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    • v.16 no.1
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    • pp.50-56
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    • 2003
  • An experiment was carried out to compare the body weight, shank length, rectal temperature, comb area, abdominal fat, blood parameters and bone traits of capon, slip and intact birds in Taiwan country chicken cockerels. One hundred and sixty-six Taiwan country chicken cockerels were randomly assigned to caponized or intact male groups. Caponized birds were surgically altered at 10 weeks old and raised to 28 weeks old. At 28 weeks of age, the capons were separated into capon and slip groups, depending on the atrophy of the comb and wattle in size. The results showed that body weight and shank length in slips were significantly (p<0.05) greater than in intact birds. Intact birds had the highest (p<0.05)feather scores and the lowest (p<0.05) rectal temperature. Compared with intact birds and slips, capons had a significantly (p<0.05) smaller comb area. Bone percentage, stress and cortical thickness, and bone and ash calcium content and plasma testosterone concentration, in intact birds were the highest (p<0.05) followed by slips and capons. However, intact birds had the lowest (p<0.05) plasma ionized calcium and phosphorus concentrations. Bone and ash manganese contents in capons were significantly (p<0.05)lower than those in others. These findings support the hypothesis that androgenic effects on secondary sexual characteristics are stronger than anabolic growth promoting response. Androgens can directly influence calcium fluxes in male chickens. Caponized caused a reduction in the bone percentage, stress, cortical thickness and bone calcium content.

Caponization Effects on Growth Performance and Lipid Metabolism in Taiwan Country Chicken Cockerels

  • Chen, Kuo-Lung;Hsieh, Tseng-Yueh;Chiou, Peter Wen-Shyg
    • Asian-Australasian Journal of Animal Sciences
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    • v.19 no.3
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    • pp.438-443
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    • 2006
  • This trial was designed to study the caponization effects on the appearance, carcass characteristics, blood constituents and lipid metabolism of Taiwan country chicken cockerels. Cockerels were caponized at 8 weeks of age. Sixteen-week-old chickens, including 10 capons, 5 slips (incomplete caponized male chickens) and 20 normal chickens of equal sexes were selected for a 10 week ad libitum feeding trial. Results showed that the testosterone concentrations in the capons and females were lower (p<0.05) than that of intact males. The comb length, height and weights were also lower (p<0.05). The weight of the slips was between that of the capons and intact males, but was heavier (p<0.05) than that of the capon. The live-weight, carcass weight and shank perimeter in the capons were higher than those of the other groups (p<0.05). Hepatic lipogenic enzyme activity analyses showed that NADP-malic dehydrogenase (MDH) activity in the capons and female chickens was higher than that in intact male chickens (p<0.05). The MDH activity in the slips was between that for the capon and intact male chickens (p>0.05). The abdominal fat weight and relative abdominal fat weight of the capons and females were heavier than that for intact males (p<0.05); the slips were between the capons and intact males. The blood lipid content results showed that the triacylglycerol and cholesterol in the capons were higher than that for intact males (p<0.05). However, the percentage of low-density lipoprotein (LDL) was lower than that in intact male chickens (p<0.05). It appears that the increase in lipid accumulation in caponized male chickens is attributed mainly to an increase in MDH activity and the changes in lipid transportation in the capons.

Reduction of superior-lateral intact mandibular condyle dislocation with bone traction hook

  • Kim, Bong Chul;Samayoa, Sara Rebeca Kang;Kim, Hyung Jun
    • Journal of the Korean Association of Oral and Maxillofacial Surgeons
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    • v.39 no.5
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    • pp.238-241
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    • 2013
  • Lateral and superior-lateral dislocations of the intact condyle are a rare complication, following traumatic insult to the mandible. We report an unusual case of a 54-year-old male patient who experienced both types of dislocations of the intact condyles with symphysis fracture following a road-traffic accident. Under general anesthesia, conventional manipulation was unsuccessful in relocating the condyles into the glenoid fossa. After applying a percutaneous traction force, using a bone traction hook placed at the sigmoid notch, the displaced intact mandibular condyles were repositioned, and the symphyseal fracture was finally reduced and fixed. The mouth opening was within normal limits, and favorable occlusion was confirmed one month postoperatively. To our knowledge, this is the first case of dislocation of both intact condyles--associated with symphysis fracture--being reduced with bone traction hook.