• Title/Summary/Keyword: Instrumental analyses

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Characteristic of Carotenoid Component from Halophilic Bacteria, Haloarcular sp. EH-1 (호염세균 Haloarcular sp. EH-1으로부터 추출한 카로테노이드 색소의 특성)

  • 정영기;최병대;강석중;정성훈;이용규;김해윤;정명주
    • KSBB Journal
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    • v.15 no.6
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    • pp.673-676
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    • 2000
  • In order to identification of carotenoid pigments of Haloarcular sp. EH-1 as a food for fish were analyzed. The content of carotenoids cultured in 3 L and 5 L bioreactor were 83.1 and 82.7 mg%, respectively. Identification of each carotenoid was achieved by means of co-TLC and co-HPLC with authentic specimens, spectroscopic and instrumental analyses, and chemical treatments as usual. The main components identified were ${\beta}$-carotene(8.1%), 3-hydroxyechinenone(42.0%) and astaxanthin(25.0%).

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The Effect of Social Network Usage on the Formation of Social Capital : Based on Twitter, Facebook, KakaoStory (소셜네트워크 활용이 사회자본 형성에 미치는 영향 : 트위터, 페이스북, 카카오스토리를 중심으로)

  • Hong, Sam-Yull;Oh, Jae-Cheol
    • The Journal of the Korea institute of electronic communication sciences
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    • v.7 no.6
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    • pp.1471-1477
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    • 2012
  • Social Network has common functions such as acting as primary platforms smoothing the sharing and distribution by combination with various contents. Hence, questionnaire has been conducted to users of all of Twitter, Facebook and KakaoStory, and the factors affected by each service are presented by statistical analyses of the survey and the results are resolved by dividing them into complete and instrumental social capital. This study will be able to provide a standard for users to select Social Network according to their purposes and contribute to development of new Social Network or improvement of existing ones.

The Development of CHANGE Flipped Learning Instructional Model in Higher Education - base on the 'educational method and technology' (대학교육에서의 CHANGE 플립러닝(Flipped Learning) 수업모형 개발 -교육방법및교육공학교과를 중심으로-)

  • JUNG, Ju-Young
    • Journal of Fisheries and Marine Sciences Education
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    • v.28 no.6
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    • pp.1834-1847
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    • 2016
  • Main objectives of the this study are: to develop a model of "Flipped Leaning" that is designed to enhance self-directed learning, learning motivation and self-control, and to verify its effectiveness-in higher education. The verification process initially concentrated on the feasibility study of the model with a thorough literature review and case analyses; then, its general and practical applicability were tested with a field study. As a result, first, the CHANGE Class Model, specifically designed for effective and efficient "Flipped Learning", was developed. It is thus named for the stages that the learning process takes place in the model-i.e., (1) Check ${\rightarrow}$ (2) Ask ${\rightarrow}$ (3) Notice ${\rightarrow}$ (4) Group presentation ${\rightarrow}$ (5) Evaluation, and it emphasizes the dynamic, questions centered (i.e. back and forth between the students and the instructor as well as between the students) learning process. Second, the Model was instrumental in enhancing self-directed learning, learning motivation and self-control; thus, as a result, it significantly improved the effectiveness, the level of concentration and the attractiveness of the learning process. The value of this study lies in pointing to a clear plan to allow a student in higher learning to set-up a self-directed learning plan, to be able to control it while being continuously motivated to complete it.

Rapid Determination of Volatile Organic Compounds in Human Whole Blood Using Static Headspace Sampling with Gas Chromatography and Mass Spectrometry

  • Lee, Ji-Young;Kim, Seungki;Lee, Jong-Tae;Choi, Jong-Ho;Lee, Jeongae;Pyo, Heesoo
    • Bulletin of the Korean Chemical Society
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    • v.33 no.12
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    • pp.3963-3970
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    • 2012
  • Headspace (HS) and headspace solid-phase microextraction (HS-SPME) were studied for extracting volatile organic compounds (VOCs) from whole blood, with chemical and instrumental variables being optimized for maximum sensitivity: incubation at $60^{\circ}C$, equilibration for 30 min, pH 11, and 2 mL injection volume. Both techniques provided accurate analyses, with detection limits of 0.05-0.1 ng $mL^{-1}$ and 0.05-0.5 ng $mL^{-1}$. HS showed better sensitivity, reproducibility, and analysis times than HS-SPME. Overall levels of chloroform in whole blood were found to be 0.05-5.84 ng $mL^{-1}$; detected levels of benzene were 0.05-2.20 ng $mL^{-1}$.

Isolation and Structural Analysis of MB4-03, an $\alpha$-Amylase Inhibitor Produced by Streptomyces sp. DMCJ-49 (Streptomyces sp. DMCJ-49 균주가 생산하는 Alpha-Amylase 저해제 MB4-03의 분리와 구조분석)

  • 염대현;최응철;김병각;김진웅
    • Microbiology and Biotechnology Letters
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    • v.18 no.4
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    • pp.338-343
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    • 1990
  • MB4-03, an $\alpha$-amylase inhibitor was isolated from the culture broth of Streptomyces sp. DMCJ-49 and purified through ion-exchange chromatography, adsorption, and gel filtration. The results of various instrumental analyses showed that the inhibitor was one of oligosaccharides that had glucoses as its major component and that its molecular weight was about 2000. And one methyl group which seemed to be related with the inhibitory activity of this compound was identified. From the CMR spectrum, it was elucidated that this compound was composed of $\alpha$ -D-glucopyranoses which were linked together by $\alpha$ (I -, 4) bond configuration. As the inhibitory effect of this compound was reduced after incubation with $\beta$-amylase, the maltose units was seemed to exist at non-reducing terminal side of it.

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Identification of New Urinary Metabolites of Byakangelicin, a Component of Angelicae dahuricae Radix, in Rats

  • Kwon, Oh-Seung;Song, Yun-Seon;Shin, Kuk-Hyun;Ryu, Jae-Chun
    • Archives of Pharmacal Research
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    • v.26 no.8
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    • pp.606-611
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    • 2003
  • Byakangelicin, 9-(2,3-dihydroxy-2-methylbutoxy)-4-methoxy-7H- furo[3,2-g][l]benzopyran-7-one (BKG), a component of Angelicae dahuricae Radix, is considered to be an inhibitor of aldose reductase for the treatment of diabetic cataract. An analytical method for the isolation of BKG developed by high-performance liquid chromatography has been reported. No literature on the metabolism of BKG, however, has been found. With the purpose of identifying new metabolites of BKG, BKG (100 mg/kg) was orally administered to Sprague-Dawley rats via a gavage. Using a metabolic cage, urine was collected for 24 h, and the urine samples were extracted by liquid-liquid extraction. For structural identification of new urinary metabolites of BKG, various instrumental analyses were conducted by gas-chromatography/mass spectrometry, high-performance liquid chromatography/diode array detector, liquid chromatography/mass spectroscopy with thermospray interface and $^1H$ nuclear magnetic resonance spectroscopy. Two metabolites produced from the Ο-demethylation or Ο-dealkylation of BKG were newly identified, and another new but unknown metabolite was assumed to be the hydroxylated form of BKG. These results indicate that the major metabolic products of BKG are formed by Ο-demethylation or Ο-dealkylation of BKG side chains.

A case study for determination of seismic risk priorities in Van (Eastern Turkey)

  • Buyuksarac, Aydin;Isik, Ercan;Harirchian, Ehsan
    • Earthquakes and Structures
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    • v.20 no.4
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    • pp.445-455
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    • 2021
  • Lake Van Basin, located in Eastern Turkey, is worth examining in terms of seismicity due to large-scale losses of property and life during the historical and instrumental period. The most important and largest province in this basin is Van. Recent indicators of the high seismicity risk in the province are damage occurring after devastating earthquakes in 2011 (Mw=7.2 and Mw=5.6) and lastly in 2020 Khoy (Mw=5.9). The seismic hazard analysis for Van and its districts in Eastern Turkey was performed in probabilistic manner. Analyses were made for thirteen different districts in Van. In this study, information is given about the tectonic setting and seismicity of Van. The probabilistic seismic hazard curves were obtained for a probability of exceedance of 2%, 10% and 50% in 50-year periods. The PGA values in the Van province vary from 0.24 g - 0.43 g for earthquakes with repetition period of 475 years. Risk priorities were determined for all districts. The highest risk was calculated for Çaldıran and the lowest risk was found for Gürpınar. Risk priorities for buildings in all districts were also determined via rapid seismic assessment for reinforced-concrete and masonry buildings in this study.

Assessment of seismic risk of a typical RC building for the 2016 Gyeongju and potential earthquakes

  • Jee, Hyun Woo;Han, Sang Whan
    • Earthquakes and Structures
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    • v.20 no.3
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    • pp.337-351
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    • 2021
  • On September 12, 2016, the Gyeongju earthquake occurred in the south-eastern region of the Korean peninsula. The event was ranked as the largest magnitude earthquake (=5.8) since instrumental recording was started by the Korean Metrological Administration (KMA) in 1978. The objective of this study is to provide information obtained from the 2016 Gyeongju earthquake and to propose a procedure estimating seismic risk of a typical old RC building for past and potential earthquakes. Ground motions are simulated using the point source model at 4941 grid locations in the Korean peninsula that resulted from the Gyeongju earthquake and from potential future earthquakes with the same hypocenter considering different soil conditions. Nonlinear response history analyses are conducted for each grid location using a three-story gravity-designed reinforced concrete (RC) frame that most closely represents conventional old school and public buildings. Then, contour maps are constructed to present the seismic risk associated with this building for the Gyeongju earthquake and potential future scenario earthquakes. These contour maps can be useful in the development of a mitigation plan for potential earthquake damage to school and public buildings at all grid locations on the Korean peninsula.

Influence of Cu Composition on the Mechanical Properties and Microstructure of Ti-Al-Si-Cu-N thick films (Ti-Al-Si-Cu-N 후막의 Cu 조성에 따른 기계적 특성과 미세구조 변화에 관한 연구)

  • Yeon-Hak Lee;Sung-Bo Heo;In-Wook Park;Daeil Kim
    • Journal of the Korean institute of surface engineering
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    • v.56 no.5
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    • pp.335-340
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    • 2023
  • Quinary component of 3㎛ thick Ti-Al-Si-Cu-N films were deposited onto WC-Co and Si wafer substrates by using an arc ion plating(AIP) system. In this study, the influence of copper(Cu) contents on the mechanical properties and microstructure of the films were investigated. The hardness of the films with 3.1 at.% Cu addition exhibited the hardness value of above 42 GPa due to the microstructural change as well as the solid-solution hardening. The instrumental analyses revealed that the deposited film with Cu content of 3.1 at.% was a nano-composites with nano-sized crystallites (5-7 nm in dia.) and a thin layer of amorphous Si3N4 phase.

Married Women's contingent Work and Work/Family Conflicts - Concentrating on Study Paper Instructors (기혼여성의 비정규 노동과 일/가족 갈등 -학습지 교사를 중심으로)

  • Kim Kyong-Hwa
    • Journal of Family Resource Management and Policy Review
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    • v.10 no.2
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    • pp.87-105
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    • 2006
  • The goal of this study is to analyze the complicated and diverse nature of the relationship between work and family life for the study paper instructors of whom are married women. The survey data for these analyses were from an in depth interview which was conducted with the 21 contingent female workers as study samples. They are the married women, who started to work or returned to work after an absence to raise a family. The results showed that in reality the contingent female worker faced worse working conditions than the full time female labour force, even if women were determined to be a study paper instructor to meet work and family demand. It was the contingent women worker with a short time work experience who were in the worst position tlo adjust their working arrangements to suit their family needs and were confronted with the greater inter-role friction as a vulnerable group. The study revealed that instrumental and emotional support of husband has a positive effect on maintaining job role and lessening role conflicts and stress of the women. The work/family relationship indicated its double side, conflict and support. The women simply could not afford to depend on the psychological support form family in the midst of the inferior employment conditions and the absence of social support. This strategy, however, was based on the family ideology and the patriarchal gender division of labor. It caused the overload, stress and poor health of women involving some risk to give up the work.

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