• Proceedings of the Zoological Society Korea Conference
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• 1995.10b
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• pp.88-88
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• 1995

Microorganism are centIal core to biosphere sm1ainablity and biogeochemical cycles on this earth. Most of food, medical and pabamceutical new materials through biotechnology are derived from many kinds of microorganisms. Microorganisms are important resources of biotechnology. Beside these, micorbial diversity is key to explore the frontiers of knowledge about the strategies and limits of life. Through the micorotganisms, we can monitor the environmental changes and conditions. Moreover, the microorganismsms play a role in conservation and restoration of higher plants and animals. And we can get a lot of ecological, evolutionary knowledges from microbial models. In spite of these importances, the microbial diversity is not properly evaluted because of their unculturablity. Only 0.001 - 3 % of total bacteria in natural habitats are cultumble and the rest are viable but uncultumble. Only 3,100 species are listed up in the Bergey's Manual. Considering the symbisis and estimated numbers of insect are more than 800,000, the symbiotic microorganisms are about 1,000,000 species. Recently, by using the genetic and molecular technics, the microbial diversity is now unveiled. In this symposium, the genetic, species and ecological diversity will be given. given.

• Korean journal of applied entomology
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• v.46 no.1 s.145
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• pp.131-139
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• 2007

From the course of screening of useful enzyme producing microorganism from insect guts, we isolated 9 lipase producing strains and their lipase producing activities were tested. 16S rDNA sequence analysis showed that they were Gram negative bacteria grouped on Serratia sp., Pseudomonas sp., and Burkholderia sp.. Among them, an excellent lipase producing strain, Burkholderia sp. HY-10 identified by 16S rDNA analysis and biochemical methods, was further studied its lipase producing characteristics. It was isolated from a longcorm beetle, Prionus insularis and showed cell density dependent lipase producing activity in the culture media that contained olive oil as a carbon source. Maximum lipase production was achieved in the M9 media containing 0.5% yeast extract and 0.5% olive oil when cultured at $30^{\circ}C$ for 36-42 hrs.

• Korean journal of applied entomology
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• v.46 no.2
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• pp.303-311
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• 2007

From the course of screening of useful xylanase producing microorganism from a phytophagous longicorn beetle, we isolated an extra-cellular xylanase producing strain, Paenibacillus sp. HY-8 from the intestine of Moechotypa diphysis adult. On the basis of morphological, biochemical and phylogenetic studies of the new isolate was identified as a Paenibacillus species. Production of xylanase in this strain was strongly induced by adding xylan to the growth medium and repressed by glucose or xylose. The highest xylanase production was attained in the M9 media containing 1% yeast extract and 0.5% birchwood xylan when cultured at $25^{\circ}C$ for 24 hrs. HY-8 producing xylanase showed superior hydrolytic activities against various plant source feedstuff than control xylanase produced by Tricoderma sp. at pH 6.0.