• 한국식품영양과학회지
• /
• 제44권9호
• /
• pp.1249-1255
• /
• 2015

본 연구는 세리신을 이용해 만든 유기셀레늄의 생체이용률을 알아보고자 하였다. 그 결과를 요약하면 다음과 같다. 항산화지표인 환원형 글루타티온은 셀레늄 처치로 인해 증가 되었는데 유기셀레늄에 의한 증가는 무기셀레늄에 비해 큰 경향이었으며, 또한 혈중 과산화 지질도 유기셀레늄이 무기셀레늄에 비해 낮은 값을 나타내어 유기셀레늄은 무기셀레늄에 비해 항산화력 향상에 더 기여하는 것으로 나타났다. 셀레늄 결핍식이로 인해 저하된 셀레늄 흡수율과 보유율은 셀레늄 처치로 증가되는데, 특히 유기셀레늄 처치로 인한 흡수율과 보유율이 높아 생체 내 이용률은 증대될 것으로 사료되며 이는 항산화력 향상에 영향을 미칠 것으로 사료된다. 혈청과 간의 셀레늄의 농도는 셀레늄 처치로 유의하게 증가되는데 셀레늄 형태 중 유기셀레늄에 의한 증가가 가장 컸으나 통계적으로 유의한 수준은 아니었다. 결론적으로 본 연구의 결과 무기질을 함유한 펩타이드는 무기질의 생체이용률을 증가시키는 것으로 나타났는데 세리신을 이용한 유기화 형태로 섭취될 경우 혈액과 장기의 침착뿐 아니라 흡수율과 보유율 증가에 관여하여 각 무기질의 효능을 증진시키는 것으로 나타났다.

• Journal of Animal Science and Technology
• /
• 제48권4호
• /
• pp.603-610
• /
• 2006

거세 한우에 셀레늄 급여원으로 무기셀레늄(sodium selenite, Sigma), 유기셀레늄(효모, 미국 Sel-Plex)군, 유기셀레늄 강화버섯을 생산하고 폐기되는 셀레늄이 다량 함유되어 있는 폐배지(Se-SMC; Se-spent mushroom compost)를 사료내 셀레늄 농도가 0.9 ppm 수준이 되도록 조절하여 처리구별 한우거세우 각각 5두를 배치하여 4개월간 급여하고 도축하여 채끝육의 육질을 비교한 결과는 다음과 같다. 가열감량은 무기셀레늄 급여구가 가장 높았고, 대조구가 유의적으로 가장 낮은 결과를 보였다(P<0.05). 전단력가는 처리구간 유의적인 차이를 보이지 않았으나, pH는 무기셀레늄 급여구가 대조구, Se-SMC 급여구보다 유의적으로 낮았다(P<0.05). 보수력과 일반조성분(수분, 단백질, 지방) 함량 및 관능검사 결과는 처리구간 통계적인 유의차는 보이지 않았다(P>0.05). 소도체 육량등급별 출현율을 비교한 결과 육량 A등급 출현율은 유기셀레늄 급여구가 가장 높았고, 그 다음으로는 Se-SMC 급여구로 나타났다. 육질등급 출현율을 비교한 결과 1+등급 출현율에서 Se-SMC 급여구가 가장 높았고, 그 다음으로는 무기 셀레늄급여구이였고, 대조구와 유기셀레늄 급여구는 가장 낮았다. 이상의 결과로 볼 때, 셀레늄 급여원은 일반조성분, 보수성, 연도에는 영향을 미치지 않는 것으로 나타났다. 무기셀레늄 급여구는 가열감량이 높고 pH가 낮아 다른 처리구에 비하여 육질이 좋지 않았다.

• Animal Bioscience
• /
• 제35권11호
• /
• pp.1725-1732
• /
• 2022

Objective: This study was conducted to investigate the effects of selenium benefits on the physiological responses, litter performance, blood profiles and milk composition of lactating sows and tissue concentration of their progeny when mixed form of selenium was provided in a lactation diet. Methods: A total of 45 multiparous sows (Yorkshire×Landrace) with similar body weight, backfat thickness, and parity were assigned to one of three treatments with 15 sows per treatment in a completely randomized design. Organic and inorganic selenium were mixed and added to the diet at 0.15 ppm and 0.25 ppm, respectively. A non-Se-fortified corn-soybean meal basal diet served as a negative control. Treatments were as follows: i) Control: corn-soybean meal based diet, ii) ISOS15: control+ inorganic Se 0.15 ppm+organic Se 0.15 ppm, iii) ISOS25: control+inorganic Se 0.25 ppm+organic Se 0.25 ppm. Results: Serum selenium concentrations of sows and piglets were increased by the supplemental Se mixture at 7 days of lactation compared with the control (p<0.01, respectively). The kidney and muscle selenium concentrations of piglets were increased by the supplemental Se mixture at 21 days of lactation compared with the control (p = 0.03; p = 0.04, respectively). Conclusion: Consequently, supplementation with mixed inorganic and organic selenium in a lactating diet could improve the selenium status of sows and piglets; no differences were observed among the mixing levels.

• Journal of Microbiology and Biotechnology
• /
• 제16권7호
• /
• pp.1041-1046
• /
• 2006

Selenium-containing peptide (selenium peptide) was produced by autolysis of total proteins of Saccharomyces cerevisiae grown with inorganic selenium. Selenium peptide exhibited antioxidant activity as a glutathione peroxidase (GPx) mimic, and its activity was dependent on the hydrolysis methods. The GPx-like activity of the hydrolyzed selenium peptide increased 2.7-folds when digested by protease, but decreased by acid hydrolysis. During the autolysis of the yeast cell, the GPx-like activity and selenium content increased 4.3- and 2.3-folds, respectively, whereas the average molecular weight (MW) of selenium peptide decreased 70%. The GPx-like activity was dependent on the MW of selenium peptide and was the highest (220 U/mg protein) at 9,500 dalton. The maximum GPx-like activity (28,600 U/g cell) was obtained by 48 h of autolysis of the cells, which were precultured with 20 ppm of selenate. Selenium peptide showed little toxicity, compared with highly toxic inorganic selenium. These results show the potential of selenium peptide as a nontoxic antioxidant that can be produced by simple autolysis of yeast cells.

• Bulletin of the Korean Chemical Society
• /
• 제31권7호
• /
• pp.1907-1914
• /
• 2010

A novel catalytic kinetic method is proposed for the determination of Se(IV), Se(VI) and total inorganic selenium in water based on the catalytic effect of Se(IV) on the reduction of bromate by p-nitrophenylhydrazine at pH 3.0. The generated bromine, $Br_2$ or $Cl_2$ plus $Br_2$ in 0.1 M NaCl (or NaBr) environment efficiently decolorized Calmagite and the reaction was monitored spectrophotometrically at 523 nm as a function of time. In this indicator reaction, bromide acted as an activator for the catalysis of selenium (IV) and a reducing agent for selenium (VI) at pH 3.0, which allowed the determination of total selenium. The fixed time method was adopted for the determination and speciation of inorganic selenium. Under the optimum conditions, the calibration graph are linear in the range 1 - 35 ${\mu}gL^{-1}$ of Se(IV) for the fixed time method at $25^{\circ}C$. The detection limit based on statistical $3S_{blank}$/m-criterion was 0.215 ${\mu}gL^{-1}$ for the fixed time method (7 min). All of the variables that affect the sensitivity at 523 nm were investigated, and the optimum conditions were established. The interference effect of various cations and anions on the Se (IV) determination was also studied. The selectivity of the selenium determination was greatly improved with the use of the strongly cation exchange resin such as Amberlite IR120 plus. The proposed kinetic method was validated statistically and through recovery studies in natural water samples. The RSDs for ten replicate measurements of 5, 15 and 25 ${\mu}gL^{-1}$ of Se(IV) and Se(VI) was changed between 2.1 - 4.85%. Analyses of a certified standard reference material (NIST SRM 1643e) for selenium using the fixed-time method showed that the proposed kinetic method has good accuracy. Se(IV), Se(VI) and total inorganic selenium in environmental water samples have been successfully determined by this method after selective reduction of Se(VI) to Se(IV).

• Asian-Australasian Journal of Animal Sciences
• /
• 제7권1호
• /
• pp.83-89
• /
• 1994

The effects of inorganic selenium (Se), selenate and selenite on Se balance levels in the different ruminal fluid fractions were studied using Japanese Corriedale wethers with an average body weight of 47 kg. A $3{\times}3$ Latin square design was used with three animal, three periods and three treatments. In each period, there was 7 d dietary adjustment followed by 5 d total collection of urine and feces. Ruminal fluid samples were obtained at 0, 1, 3, 5 and 7 h postprandially on the final day of the collection period. The three dietary treatments were: (1) without Se supplementation (control); (2) with Se supplement as sodium selenate; and (3) sodium selenite at a rate of 0.2 mg Se/kg dietary DM. The basal diet was timothy hay (Phleum pratense L.) fed 2% of body weight/d. Results indicated that Se balance were higher (p < 0.05) for those animals under supplementation than those animals under control. Overall data gathered showed a similar digestion balance of selenate and selenite in sheep. Inorganic Se, both selenate and selenite produced positive Se contents of the ruminal feed particles and protozoa. Bacterial Se increased (p < 0.05) on the first three hours post-prandially in Se supplemented diets. Gross ruminal fluid fraction, although there was improvement on their Se content under the supplemented diets, the changes were insignificant over the control. free inorganic Se and Se in soluble protein of the ruminal fluid were not significantly different for selenate and selenite. Most of the Se in the ruminal fluids of the animals under supplementation were insoluble, indicating the influence of rumen environments on Se bioavaliability.

• Asian-Australasian Journal of Animal Sciences
• /
• 제17권7호
• /
• pp.1000-1006
• /
• 2004

Selenium and vitamin E are micronutrients essential for normal health and maintenance in poultry. They are necessary in preventing free radical damage to phospholipid membranes, enzymes and other important molecules. Two experiments were conducted in a semi-commercial environment to examine the effect of Se source and vitamin E level in diet on broiler performance and meat quality. Increasing vitamin E from 50 IU to 100 IU did not affect growth performance of broilers although the 24 h drip-loss was tended to be reduced (p=0.06). There was an interaction between vitamin E and the source of Se in glutathione peroxidase activity (GSH-Px) and Se concentration in excreta. Increasing vitamin E from 50 IU to 100 IU elevated GSH-Px and Se concentration in excreta by 42 IU/g Hb and 0.9 ppm for the organic Se group, respectively, but reduced GSH-Px and Se concentration in excreta by 16 IU/g Hb and 1.3 ppm for inorganic group, respectively. Vitamin E played no role in the feather coverage of the birds when scored on day 37. Organic Se is more effective in improving feather score and 24 h drip-loss, with a markedly higher deposition rate in breast muscle and a lower excretion rate in the excreta (p<0.05) compared to the inorganic Se source. Both vitamin E and the source of Se did not affect (p>0.05) the energy utilisation by birds.

• 한국축산식품학회지
• /
• 제40권3호
• /
• pp.338-349
• /
• 2020

The effects of organic Zn-L-selenomethionine (Zn-L-SeMet) at 0.3 ppm on carcass composition, meat characteristics, fatty acid composition, glutathione peroxidase activity, and ribonucleotide content were compared against the commercial inorganic sodium selenite (Na-Se) and the combination of the two, in commercial broilers. A total of 540 one day-old chicks were assigned at random to 3 dietary treatments : i) commercial inorganic selenium as control or T1, ii) a 1:1 ratio of inorganic and organic selenium as T2, and iii) organic selenium as T3. Carcass composition, meat characteristics, cholesterol content, fatty acid composition, and ribonucleotide content were generally unaffected by treatments. However, discrepancy were significantly observed in glutathione peroxidase activity (GSH-Px) and water holding capacity, with organic selenium showing higher glutathione peroxidase activity (p<0.01) and lower shrinkage loss (p<0.05), respectively. These findings could be explained by the contribution of organic selenium in bioavailability of GSH-Px. However, having conducted in a commercial close house system with sufficient amount of nutritional supplementation, the present study demonstrated little or no effects of organic Zn-L-SeMet on meat characteristics, fatty acid composition, and ribonucleotide content (flavor characteristic) in broiler chickens.

• Animal Bioscience
• /
• 제36권7호
• /
• pp.1083-1090
• /
• 2023

Objective: This study was conducted to evaluate the short-term effects of dietary selenium supplementation on lactating sows on the physiological response, litter performance, milk composition, and tissue selenium retention in piglets when selenium was provided by different sources and at different levels in a lactation diet. Methods: A total of 48 multiparous sows (Yorkshire×Landrace) with average body weight, backfat thickness, and parity were assigned to one of the four treatments with 12 sows per treatment using a 2×2 factorial arrangement in a completely randomized design. Inorganic or organic Se sources were added to the diet at 0.30 ppm and 0.50 ppm Se. Treatments were as follows: i) IS30, basal diet + inorganic Se 0.30 ppm; ii) IS50, basal diet + inorganic Se 0.50 ppm; iii) OS30, basal diet + organic Se 0.30 ppm; and iv) OS50: basal diet + organic Se 0.50 ppm. Results: At Day 21 of lactation, a high tendency of litter weight (p = 0.08) and litter weight gain (p = 0.09) were observed when sows were fed an organic Se source. The milk Se concentration in the organic Se treatment was higher than that in the inorganic Se treatment at Day 21 of lactation (p<0.05). The serum Se concentrations of sows and piglets at Day 21 of lactation were significantly higher when lactating sows were fed organic Se instead of inorganic Se (p<0.01). During the suckling period, the kidney and muscle Se concentrations of piglets at Day 21 of lactation were significantly higher when the sow dietary Se source was organic (p<0.05). Liver Se concentrations were affected by Se source and level (p<0.05). This also resulted in an interaction response at 21 days of lactation (p<0.05). Conclusion: The supplementation of dietary organic Se in a lactating diet could improve sow feed consumption, piglet performance, milk Se level, and the Se status of sows and piglets.

• Asian Pacific Journal of Cancer Prevention
• /
• 제17권5호
• /
• pp.2527-2533
• /
• 2016

Sensitising cancer cells and at the same time desensitizing normal cells is a double task in cancer management. Agents which can combat the debilitating side effects of cancer therapeutics and simultaneously synergize with anticancer agents in specifically targeting cancer cells are needed. Selenium, a proven anticarcinogen, gains due importance in terms of its efficacy to combat the side effects of cancer therapy. This study is a comparative analysis of the chemoprotective effects of selenium compounds, methyl selenol (generated from organic selenomethionine (5mmol/L ; METase 40U/L)) and sodium selenite (inorganic form)($30{\mu}M$) in peripheral blood human lymphocytes exposed to cisplatin and mitomycin. Biochemical alterations occurring in many cells during apoptosis include loss of plasma membrane phospholipid asymmetry, DNA fragmentation, and activation of caspase-3. The present study demonstrated that the selenium metabolite and selenite are efficient in protecting lymphocytes undergoing DNA damage and exerted their activity by reducing caspase 3 expression. Interestingly organic methylselenol (MeSe) was found to offer more protective effects compared to inorganic selenite (SeL), by reducing the induction of apoptosis by the cytotoxic agents. This suggests that MeSe and to a lesser extent selenite might have potential for assessment in clinical trials and could be considered as strong candidates in pharmacogenomics or in the nutriprotective arena.