• 한국폐기물자원순환학회지
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• 제34권5호
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• pp.474-481
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• 2017

This study was conducted to investigate the effects of inoculum and carbon sources on anaerobic digestion characteristics. The treatments were combinations of inoculum (digestate of cattle manure and rumen fluid) with carbon sources (starch, cellulose, and xylan). Anaerobic digestion was performed in triplicate at $37^{\circ}C$ for 18 days at 100 rpm. Sampling was performed at 0, 1, 2, 3, 4, 5, 7, 9, 12, 15, and 18 days to measure pH, ammonia-N, volatile solids reduction, the cumulative methane content, and the cumulative methane production. There was a significant difference in methane content depending on the carbon source and there was a significant difference in pH, ammonia-N, methane production, and methane content depending on the inoculum (P < 0.05). The results of methane production were higher in the digestate of cattle manure treatment than in the rumen fluid treatment (P < 0.05). In this study, different digestive patterns depending on the type of carbon source could be used as basic research data to set the hydraulic residence time of anaerobic digestion facilities. In addition, the use of ruminal fluid as an inoculum may help accelerate the hydrolysis and acid production steps.

• 한국식물병리학회지
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• 제11권4호
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• pp.344-347
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• 1995

Factors affecting on the ascospore release of Mycosphaerella nawae, the causal organism of persimmon leaf casting disease, were investigated. The ascospore release of the pathogen occurred following the precipitation of rain. The ascospore release started from the beginning of raining, reached maximum at 2 hours after the precipitation of rain, and then decreased abruptly. When the inoculum source (a file of infected leaves) was submerged in water to imitate raining conditions, 92.5% of the total ascospores were released within 1 hour after submerging, 5.8% were after 2 hours, 1.4% were after 4 hours, 0.1% were after 8 hours, and none detected after 10 hours. The inoculum source overwintered in the field released ascospores much more and earlier than the inoculum source kept in the greenhouse. The first ascospore release was about 10 days earlier, and the amount of the total liberated spores was 3∼4 times higher in the field inoculum source than the greenhouse nioculum source. The early defoliated leaves (in early October) in the previous year produced ascospores twice more than the late defoliated leaves (in early November) produced.

• 한국식품영양학회지
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• 제11권6호
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• pp.606-611
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• 1998

For waste recyle, we were investigated on Gardenia blue color production using Gardenia by-product by Bacillus subtilits. Optimum conditions for producing blue pigment were found to be 30$^{\circ}C$, initial pH 6.5, glucose as a carbon source 3% and yeast extract as a nitrogen source 0.5%, respectively. Optimum conditions for fermentor culture were agitation speed 400rpm, aeration 2 vvm and inoculum 5%. The optimum perculture time for inoculum was 20 hrs for blue pigment production.

• 한국식물병리학회지
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• 제14권5호
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• pp.393-396
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• 1998

The characteristic Ramularia type conidia of Mycosphaerella nawae were formed on naturally infected leaves. Artificial inoculation with the conidia induced typical symptom on leaves, which was not distinguishable from those of ascospore infection, which has been considered as a primary inoculum source in nature. Also the morphology of the conidia produced on PDA was not different from those formed on artificially inoculated leaves or on naturally infected leaves at later stage of symptom development. Accordingly, we report the role of the conidia as a secondary inoculum of the circular leaf spot pathogen of persimmon for the first time.

• 유기물자원화
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• 제22권2호
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• pp.57-66
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• 2014

침출수 순환형 음식물류 폐기물 혐기성 소화 공법은 기존의 일상 혐기성 소화 공법에 비해 투입되는 유기물 부하량 대비 발생하는 메탄가스 수율이 높다는 장점이 있다. 본 연구는 분리 수거된 교내 기숙사 식당 음식물류 폐기물과 하수처리장 혐기성 소화조의 혐기성 미생물을 이용하여 신재생 에너지인 메탄가스를 더욱 효율적으로 얻기 위해 음식물류 폐기물/혐기성 슬러지 비를 달리하여 실험을 실시하였다. 음식물류 폐기물/혐기성 슬러지 비가 2:8이고 유기물 부하율이 9 gVS/L인 침출수 순환형 음식물류 혐기성 소화 반응조의 바이오 가스 발생이 가장 높았다. 또한 이 혐기성 반응조에서 발생하는 바이오가스 내부의 $H_2S$와 $NH_3$가 지속적으로 감소하였고 바이오 가스의 수율은 평균 $1.395m^3$ Biogas/kg VS added로 나타났다. 이에 비해 나머지 음식물류 폐기물/혐기성 슬러지 비가 3:7, 8:2인 실험의 반응조에서는 바이오 가스 발생이 멈추었다.

• 한국응용곤충학회지
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• 제22권4호
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• pp.277-282
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• 1983

엽위별로 형성된 병반수나 병반면적율은 통일계품종에 있어서는 n-1에서 n-3까지 그리고 일본형수수품종에 있어서는 n-2에서 많았으나 통일계품종중에서도 고도로 감수성인 노풍에 있어서는 n-1(지엽)에 가장 많이 분포되어 있었다. 병반의 분포도와 시비수준과의 관계는 시비수준이 높아질수록 뚜렸하게 나타나는 경향을 보여주고 있다. 엽위별로 보면 수잉기 이후 이삭도열병의 전염원으로는 n-1에서 n-5까지의 잎위의 병반이 관여하나 주로 n-1엽과 n-2엽의 관여도가 큰 것으로 고찰되었다. 수잉기 이후의 저온역에서의 변온$(24^{\circ}C\~16^{\circ}C)$은 잎위의 병반에서의 포자 형성량을 증가시키며 포자의 형성, 이탈, 비산에 아무런 지장을 주지않고 9월말까지도 다량의 전염원을 수에 공급하고 있었다.

• Asian-Australasian Journal of Animal Sciences
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• 제19권11호
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• pp.1649-1657
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• 2006

In order to find an alternative source of inoculum to caecal content for studying the fermentation activity of rabbit hindgut, caecal content and faeces of 25 hybrid Hyla rabbits were used as inocula for an in vitro gas production trial. About 1 g of three substrates (dehydrated alfalfa meal, dehydrated beet pulp, barley) was weighed, in quadruplicate per inoculum, in 120 ml bottles; 75 ml of anaerobic medium and 4 ml of reducing solution were added and bottles were placed at $39^{\circ}C$. Caecal content and faeces were diluted respectively 1:2 (CI) and 1:8 (FI) with anaerobic medium and were introduced in the respective bottles (10 ml). Gas production was recorded 20 times at 2-24 h intervals throughout fermentation (96 h). The fermentation characteristics (i.e. degraded organic matter, OMd; potential gas production, A; fermentation rate, Rmax; time at which it is reached, Tmax; pH, volatile fatty acid, VFA) were studied by inoculum and feedstuffs. The feedstuffs, according to their chemical composition, showed very different fermentation characteristics. In particular, OMd, A and Rmax allowed feedstuff classification as follows: barley>beet pulp>alfalfa. The inocula differ (p<0.05) in Tmax, were higher for CI (15.53 vs. 11.96 h) and in VFA production. In particular, CI produced higher levels of acetate (38.9 vs. 33.4 mM/g OM incubated, p<0.01) and isobutyrate (0.72 vs. 0.42, p<0.01) but less propionate (7.1 vs. 10.3, p<0.01) and butyrate (11.3 vs. 14.0, p<0.01). However, the trend of gas production, similar for the inocula according to the fermented substrate, and the good regression equation to estimate some caecal fermentation parameters from faeces suggest that, after standardisation, the faeces could be used as an alternative inoculum for gas tests in rabbit.

• Asian-Australasian Journal of Animal Sciences
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• 제9권4호
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• pp.375-379
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• 1996

The experiment investigated the possibility of using effluent from RUSITEC (rumen simulation technique) inoculated with rumen liquor or cow faeces as sources of micro-organisms for in vitro digestion of forages. Nine forages ${\times}3$ sources of inoculum were used in a factorial arrangement of treatments. Rumen liquor was collected from fistulated sheep and faeces was collected from cows. The RUSITEC apparatus consisted of 4 vessels, 2 vessels were charged with faecal liquor and 2 with rumen liquor. On the 8th day of the experiment RUSITEC effluent were collected to use in in vitro studies. In vitro OMD (g/kg) values using three sources of inoculum (fresh rumen liquor, RUSITEC effluent from rumen liquor or cow faeces) were statistically significant (p < 0.001). The regression relationships between OMD using fresh rumen liquor and RUSITEC effluent were highly significant ($R^2>0.90$). The results suggest that RUSITEC effluent either from rumen liquor or cow faeces can be used as a source of micro-organisms for in vitro digestion of forages.

• International Journal of Industrial Entomology and Biomaterials
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• 제6권2호
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• pp.163-169
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• 2003

Studies were conducted on the effect of pruning time, host age, conidial dispersal and weather parameters on the incidence and severity of mulberry leaf spot (Myrothecium roridum). The disease severity (%) increased with increase in shoot age irrespective of pruning date. Maximum disease severity was observed in plants pruned during first week of April and minimum disease severity in plants pruned during first week of March. Significant (P ＜ 0.01) influence of date of pruning, shoot age and their interaction was observed on severity of the disease. Apparent infection rate (r) was significantly higher during the plant growth period from day 48 to day 55. Average apparent yale was higher in plants pruned during first week of April and least in plants pruned during first week of July. The disease infection was negatively correlated to distance from the inoculum source. Leaf spot severity (%) was influenced by weather parameters. Multiple regression analysis revealed contribution of various combinations of weather parameters on the disease severity. Linear prediction model $(Y ＝ －81.803＋1.176x_2＋0.765x_3) with significant $R^2$ was developed for prediction of the disease under natural epiphytotic condition.

• 한국미생물·생명공학회지
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• 제20권5호
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• pp.527-533
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• 1992

효모탈묵용 cellulase와 xylanase 생산을 위한 조건을 조사한 결과 배지초기 pH, Tween 80, 포자접종량, 질소원의 종류 및 탄소원의 종류에 따라 효소 생산량이 크게 변하였다. Cellulase 생산을 위한 최적조건으로는 pH5.0-6.5, Tween 80 0.02, 포자현탄액($1{\times}10^{7}$/ml) 접종량 0.5-1.0, 질소원으로 면실박, 탄소원으로 옥분을 사용하였을 때 가장 효과적 이었다. 또한, xylanase 생산을 위한 최적조건으로는 pH6.5, Tween 80 0.01%, 질소원으로 corn steep liquor, 탄소원으로는 신문고지를 사용하였을 때 효과적이었으며, 이때 포자현탁액의 접종량은 cellulase 생산의 경우와 동일하였다.한편, cellulasedhk xylanase의 동시생산을 위해서는 플라스크 규모에서 질소원으로 면실박 0.5%, 탄소원으로 신문고지 1.0%와 옥분 2.0%를 첨가하였을 때 효과적이었으며, 이때 생상된 배양약의 cellulase의활성은 6.11~7.22IU/ml, xylanase의 활성은 97.7IU/ml이었다. 그러나 fermentor 규모에서는 동일 배지조성에서 효소생산이 다소 감소하였으며, 특히 xylanase 경우 배양액의 효소활성이 낮아서 이에 대한 보완이 요구된다.