• Title/Summary/Keyword: Inner surface

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Next-generation sequencing for the genetic characterization of Maedi/Visna virus isolated from the northwest of China

  • Zhao, Ling;Zhang, Liang;Shi, Xiaona;Duan, Xujie;Li, Huiping;Liu, Shuying
    • Journal of Veterinary Science
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    • v.22 no.6
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    • pp.66.1-66.9
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    • 2021
  • Background: Maedi/Visna virus (MVV) is a contagious viral pathogen that causes considerable economic losses to the sheep industry worldwide. Objectives: In China, MVV has been detected in several regions, but its molecular characteristics and genetic variations were not thoroughly investigated. Methods: Therefore, in this study, we conducted next-generation sequencing on an MVV strain obtained from northwest China to reveal its genetic evolution via phylogenetic analysis. Results: A MVV strain obtained from Inner Mongolia (NM) of China was identified. Sequence analysis indicated that its whole-genome length is 9193 bp. Homology comparison of nucleotides between the NM strain and reference strains showed that the sequence homology of gag and env were 77.1%-86.8% and 67.7%-75.5%, respectively. Phylogenetic analysis revealed that the NM strain was closely related to the reference strains isolated from America, which belong to the A2 type. Notably, there were 5 amino acid insertions in variable region 4 and a highly variable motif at the C-terminal of the surface glycoprotein (SU5). Conclusions: The present study is the first to show the whole-genome sequence of an MVV obtained from China. The detailed analyses provide essential information for understanding the genetic characteristics of MVV, and the results enrich the MVV library.

Wear Analysis of the Ti-N Coated Punch in Piercing According to the Volume of Production (생산수량에 따른 Ti-N 코팅 펀치의 마멸해석)

  • 황상홍;고대철;김병민
    • Journal of the Korean Society for Precision Engineering
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    • v.17 no.3
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    • pp.149-157
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    • 2000
  • Tool wear in the shearing process such as blanking, piercing and trimming is very important, because it has great effects on the dimensional accuracy, working efficiency and economy. Most of tools in the shearing process have the coated layer at surface fur good wear and corrosion resistance. When the surface of tool is teated, the wear Phenomena of coated surface layer and inner layer may be different. This paper describes a computer modelling technique by the finite element method in order to investigate the wear mechanism and to predict the wear profile of Ti-N coated tool in piercing process according to the volume of Production. Wear coefficients of the coated layer and inner layer are obtained through Pin-on-Disk wear test, respectively. To verify the effectiveness of the suggested technique, the technique is applied to wear analysis in piercing recess of piston pin and simulation results are compared with experimental ones.

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The study on characteristics of corona ignitor for surface treatment of insulator (절연재료의 표면개질을 위한 코로나 발생기의 특성에 관한 연구)

  • ;;;;Y.Tabata;J.S Chang
    • Electrical & Electronic Materials
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    • v.8 no.4
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    • pp.504-508
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    • 1995
  • The purpose of this research is to study on characteristics of corona ignitor which was designed for ignition of plasma, used at hard coating, surface treatment and thin film preparation, at high pressure. Corona ignitor composed of hollow type inner electrode and ring type external electrode. Though corona voltage increased with increasing distance between electrodes, corona discharge can be controlled stably. The gas flow in hollow type inner electrode and the construction between electrodes affect a length of corona flame and corona phenomenon. It is possible to ignite the . plasma, usually generated at low pressure(10 Torr), at high pressure(100 Torr) by corona ignitor.

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Evaluation on the Properties of Strain Distribution of the sopt welding specimen under tensile-shear load (인장-전단하중을 받는 점 용접재의 변형률 분포 특성 평가)

  • 김덕중
    • Journal of the Korean Society of Manufacturing Technology Engineers
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    • v.8 no.6
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    • pp.113-118
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    • 1999
  • In order to evaluate strength of spot welded joint, at first it is importent that we should know strain distribution near nugget zone. During loading, in HAZ, compressive strain increase with Increase of load, but in nugget zone tensile strain increase. During unloading, on the other hand, even through the decreases, the strain variation is not almost appeared in nugget zone and HAZ. In nugget boundary zone, the strain range increases continuously along with load increase on outer surface, but the strain increases continunously and decreases rapidly beyond yield strength on inner surface. In this paper, strain distribution are measured in inner and outer surface with variation of thickness and load under tensile-shear load. Tensile-shear strength increased as with increase of specimen thickness. As for thickness increase rates are 25%, 50%, 100%, and 150%, tensile-shear strength in crease rates are 40%, 81%, 130% and 228%.

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Ultrastructure of the fertilized egg envelopes in Ancistrus cirrhosus, Loricariidae, Teleostei

  • Dong Heui Kim
    • Applied Microscopy
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    • v.50
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    • pp.13.1-13.7
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    • 2020
  • We examined the morphology of fertilized egg and ultrastructures of fertilized egg envelopes of Ancistrus cirrhosus belong to Loricariidae using light and electron microscopes. The fertilized eggs formed a mass on the spawning place and were yellowish, spherical, non-transparent, demersal, adhesive, and a narrow perivitelline space. But, the adhesiveness of fertilized eggs was disappeared after spawning excluding contact parts. The micropyle with funnel shape was surrounded by 15-19 furrow lines of egg envelope in a spoke-like pattern. The outer surface of egg envelope has smooth side and inner surface of egg envelope was rough with grooves. Also, the total thickness of the fertilized egg envelope was about 32.58 ± 0.85 ㎛ (n = 20), and the fertilized egg envelope consisted of three layers, an outer adhesive electron-dense layer, a middle layer with low electron density and an inner electron-dense layer with grooves in counter structure from other most teleost. Collectively, these morphological characteristics and adhesive property of fertilized egg, and ultrastructures of micropyle, outer surface, and section of fertilized egg envelope are showed species specificity.

Heat Transfer Experiment and Analysis to Predict the Efficiency of Heat Exchanger for Deep Geothermal System (심부지열 용 동축 열교환기 성능예측을 위한 열전달 실험 및 해석)

  • Jung, Kuk-Jin;Jeong, Yoon-Seong;Park, Jun Su;Lee, Dong Hyun
    • Journal of Institute of Convergence Technology
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    • v.7 no.1
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    • pp.1-6
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    • 2017
  • The Heat exchanger for deep geothermal system is very important to enhance the efficiency of the system. The co-axial heat exchanger is used due to the limitation of digging space. The heat transfer on the external surface of outer pipe should be high to receive a large amount of heat from the ground. However, the inner pipe should be insulated to reduce the heat loss and increase the temperature of discharge water. This study made experiment apparatus to describe the co-axial heat exchanger and measure the heat transfer coefficients on the internal and external surface. And the pin-fin was designed and fixed on the internal surface to increase the efficiency of heat exchanger. Finally, we calculated the temperature of discharge water using the heat transfer circuit of co-axial heat exchanger and heat transfer coefficient which from experimental results. The water temperature was reached the ground temperature at -500 m and following the ground temperature. When the water return to the ground surface, the water temperature was decreased due to heat loss. As the pin-fin case, the heat transfer coefficient on the internal surface was decreased by 30% and it mean that the pin-fin help to insulate the inner pipe. However, the discharge water temperature did not change although pin-fin fixed on the inner pipe.

An Application of the Localized Finite Element Method to Two-dimensional Free Surface Wave Problems (2차원 자유표면파 문제에서의 국소 유한요소법의 응용)

  • Hyun-Kwon,Kil;K.J.,Bai
    • Bulletin of the Society of Naval Architects of Korea
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    • v.22 no.3
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    • pp.9-18
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    • 1985
  • The numerical calculation for solving boundary-value problem related to potential flows with a free surface is carried out by application of the localized finite element method. Only forced motion of 2-D body in infinitely deep fluid is considered, although this schemes is equally applicable to any first order time-harmonic problems of similar nature. The infinite domain of the fluid is separated into the inner flow field and the outer flow field with common inter-surface boundary. The finite element method is applied to obtain the solution in the inner flow field and the Green functions are utilized to represent the solution in the outer flow field. At the inter-surface boundary, the continuity of the value of potential and the normal derivative of the potential(i.e. matching condition) is conserved. The present method has better computational efficiency than the previous LFEM and the integral equation method of Frank. This enhanced computational efficiency is presumably due to the fact that the present method gives a symmetric coefficient matrix and requires less computational time in calculating the influence coefficient matrix of Green function than the integral equation method. And the irregular frequency desen't exist because the uniqueness of the solution is assured by the such that the exact free surface condition is satisfied on the boundary of the localized finite element region(i.e. inner region). As an example of the above method, the hydrodynamic forces for the circular cylinder and the rectangular cylinders are calculated. In the computed results, the small number of singularity distribution segments($3{\sim}6$) give good result relative to Ursell's and Vugts'.

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A Study on the Optimization of Ballast Water Treatment System for Scale-up (선박평형수 처리장치의 Scale-up을 위한 조건 최적화 연구)

  • Kang, Ahyoung;Song, Ju Yeong
    • Korean Chemical Engineering Research
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    • v.54 no.5
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    • pp.630-635
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    • 2016
  • In the prior work, we studied a ballast water treatment apparatus, which is secondary pollution free by using physical treatment of shear stress. The principle of this apparatus is smashing various microorganisms by shear stress generated between stationary outer cylinder and revolving inner cylinder. Because of various magnitude of shear stress according to the inner cylinder surface type and revolution speed, an appropriate surface type and optimum revolution speed should be studied by consecutive experiment to determine the reference data for commercial apparatus. Through a comparative study of disk type and cylinder type of ballast water treatment apparatus, cylinder type is turned out to be superb to disk type. In this study, we studied to determine the superior collaboration of cylinder type, groove type and knurling type of inner cylinder to non patterned outer cylinder, and to optimize the revolution speed and flow rate according to the gap between inner cylinder and outer cylinder. As a result, we could get perfect sterilization effect at groove type under the conditions of 250 mL/min of flow rate at 8,000 rpm and 500 mL/min of flow rate at 10,000 rpm respectively.

Optimal Design of the Safety Valve by Response Surface Method (반응표면법을 이용한 안전밸브의 최적화)

  • Lee, Sang-Woo;Shin, Dae-Young;Byun, Cheol-Woong
    • Journal of the Computational Structural Engineering Institute of Korea
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    • v.20 no.5
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    • pp.551-556
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    • 2007
  • High pressure storage of the agent gas in fire suppression system was composed of tank, main valve and safety valve, which prevents the fracture of the high pressure storage. The safety valve has circular thin plate as fracture plate that was destroyed over fracture pressure. When inner pressure of the storage is reached the fracture pressure, the safety valve discharges gas and degrades simultaneously the inner pressure of the storage. There are design variables such as flow path diameter, inner diameter of the plastic packing ring, thickness of plate and fillet radius. In this variables, thickness of plate is set to be a value of 0.2mm. The main effect of variables on the inner pressure, has been decided using factorial design and statistical analysis. Therefore, the relation of variables are expressed by regression equation. It is disclosed results that the difference of fracture pressures between the equation and experiment has $2{\sim}5%$. Finally, using response surface method, the optimal design of the safety valve could be decided with safety pressure of 25MPa, where the fracture occurs on circular thin plate.

Environmental Improvement in a Welding Factory by the Jet Ventilation System (제트 환기 시스템 도입에 의한 플랜트 기자재 용접장의 환경 개선 효과 분석)

  • Lim, Jung-Ho;Lee, Tae-Gu;Moon, Seung-Jae;Lee, Jae-Heon
    • Plant Journal
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    • v.4 no.2
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    • pp.66-72
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    • 2008
  • In this inquiry, I would suggest jet ventilation system for effective elimination of welding flaw at machinery material welding shop on plant and evaluate the airstream on inner space and property of welding flaw's density through the examination. We can know outer atmosphere inflows at the speed of about 0.05m/s from western entrance in case of stopping the jet ventilation system, but airstream is accumulated on entire space. At height of worker's breathing surface(Ground Level = 1.5 m, below of GL) and welding work center, density of welding flaw on upper part(GL = 12m) is appeared 4 times higher than outer atmosphere at surplus range besides nearby of western entrance. At operation of jet ventilation system, since the smooth air current transfer at inner space and exhaust effect the wind speed is maintained at 0.932 m/s at the point of height of worker's breathing surface on inner space and it's concluded about the working conditions have been better than before operation of jet ventilation system because of that results show that inner space density of welding flaw at height of worker's breathing surface is 40.5%, and in the work shop, it is 20.3% at upper part.

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