• Title/Summary/Keyword: Injection level

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EFFECT OF GONADOTROPHINS ON FOLLICULAR STEROID HORMONE PRODUCTION IN HYPOPHYSECTOMISED HENS (Gallus domesticus)

  • Li, Z.D.;Koga, O.;Tanaka, K.;Fujihara, N.
    • Asian-Australasian Journal of Animal Sciences
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    • v.6 no.3
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    • pp.411-416
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    • 1993
  • We assessed effects of ovine luteinizing hormone (oLH) and follicle-stimulating hormone (oFSH) on the granulose and theca layers from the four largest follicles, $F_1-F_4$ of hens which had been hypophysectomized 12 h before expected ovulation. Ovine LH (0.4 mg), oFSH (0.4 mg) or oLH in combination with oFSH (0.4 mg each) was injected intravenously 6 h after hypophysectomy. Progesterone, testosterone and $estradiol-17{\beta}$ levels of the granulose and theca layers which were removed 6 h after hormone injection, were measured by radioimmunoassay. Progesterone contents of $F_1-F_3$ granulosa layer at 12 h after hypophysectomy were much lower than those of control hens. This reduced progesterone level was restored partially by the injection of oLH alone for $F_1$, while no follicles responded to oFSH treatment. In contrast, the injection of oLH in combination with oFSH resulted in high progesterone content of the granulose layer from all four follicles. Progesterone content of the theca layer was negligible in all treatments. Simultaneous injection of oLH and oFSH also elevated $estradiol-17{\beta}$ level accumulating in the theca layer from all follicles, of which much higher concentrations of $estradiol-17{\beta}$ were observed when comparison were made to each of their corresponding controls. No appreciable change in testosterone contents of two layers was observed in the present experiments. These results suggest that oFSH augments function of oLH to stimulate the production of progesterone in the granulose layer and $estradiol-17{\beta}$ in the theca layer.

A Study on the analytical derivation of the L-I-V characteristics for a SCH QW Laser Diode (SCH 양자우물 레이저 다이오드에 대한 L-I-V 특성의 해석적도출에 관한 연구)

  • Park, Ryung-Sik;Bang, Seong-Man;Sim, Jae-Hun;Seo, Jeong-Ha
    • Journal of the Institute of Electronics Engineers of Korea SD
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    • v.39 no.3
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    • pp.9-19
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    • 2002
  • By using the thermionic emission model, the L-I-V(power-current-voltage) characteristics of a SCH(seperate confinement heterostructure) QW(quantum well) laser diode is analytically derived. We derived the relationships between the bulk carrier density of SCH regions and the confined carrier density of QW. The L-I-V characteristics is derived analytically by using current continuity equations. Solving the ambipolar diffusion equation under the condition of high level injection and charge neutrality, the current distribution in the SCH regions is considered. Results showed that the major factor affecting the laser I-V characteristics was the change of potential barrier at the cladding-SCH interface. Also the series resistance of a laser diode was decreased and the carrier injection was increased by increasing the forward flux of injection current from cladding to SCH region.

17Beta-Estradiol Regulates NUCB2/Nesfatin-1 Expression in Mouse Oviduct

  • Sun, Sojung;Shin, Jungwoo;Jang, Jiwon;Hwang, Seungyeon;Kim, Jeongwoo;Kong, Jinseong;Yang, Hyunwon
    • Development and Reproduction
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    • v.24 no.1
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    • pp.43-52
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    • 2020
  • NUCB2/nesfatin-1 known to regulate appetite and energy homeostasis is expressed not only in the hypothalamus, but also in various organs and tissues. Our previous reports also demonstrated that NUCB2/nesfatin-1 was expressed in the reproductive organs, including the ovaries, uterus, and testes of mice. However, it is yet known whether NUCB2/nesfatin-1 is expressed in the oviduct and how its expression is regulated. Therefore, we investigated the expression of NUCB2/nesfatin-1 in the oviduct and its expression is regulated by gonadotropin. Immunohistochemical staining results showed that nesfatin-1 protein was localized in epithelial cells of the oviduct. As a result of quantitative real-time PCR (qRT-PCR) and Western blot, NUCB2/nesfatin-1 was detected strongly in the oviducts. During the estrus cycle, NUCB2/nesfatin-1 expression in the oviducts was markedly higher in the proestrus stage than in other estrus stages. In order to elucidate whether the expression of NUCB2 mRNA is controlled by the gonadotropins, we injected PMSG and hCG and measured NUCB2 mRNA level in the oviduct after injection. Its level was increased in the oviduct after PMSG injection, but no significant change after hCG injection. In addition, NUCB2 mRNA levels were markedly reduced after ovariectomy, while recovered after 17β-estradiol (E2) injection, but not by progesterone (P4). This study demonstrated that NUCB2/nesfatin-1 is highly expressed in the oviduct of mouse and its expression is regulated by E2 secreted by the ovaries. These results suggest that NUCB2/nesfatin-1 expressed by the oviduct may affect the function of the oviduct regulated by the ovaries.

Prediction of Cement Volume for Vertebroplasty Based on Imaging and Biomechanical Results

  • Lee, Sung-Jae;Tack, Gye-Rae;Lee, Seung-Yong;Jun, Bong-Jae;Lim, Do-Hyung;Shin, Jung-Woog;Kim, Jeong-Koo;Shin, Kyu-Chul
    • Journal of Mechanical Science and Technology
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    • v.15 no.7
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    • pp.1041-1050
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    • 2001
  • Control of bone cement volume (PMMA) may be critical for preventing complications in vertebroplasty, the percutaneous injection of PMMA into vertebra. The purpose of this study was to predict the optimal volume of PMMA injection based on CT images. For this, correlation between PMMA volume and textural features of CT images was examined before and after surgery to evaluate the appropriate PMMA amount. The gray level run length analysis was used to determine the textural features of the trabecular bone. Extimation of PMMA volume was done using 3D visualization with semi-automatic segmentation on postoperative CT images. Then, finite element (FE) models were constructed based on the CT image data of patients and PMMA volume. Appropriate material properties for the trabecular bone were assigned by converting BMD to elastic modulus. Structural reinforcement due to the changes in PMMA volume and BMD was assessed in terms of axial displacement of the superior endplate. A strong correlation was found between the injected PMMA volume and the area of the intertrabecular space and that of trabecular bone calculated from the CT images (r=0.90 and -0.90, respectively). FE results suggested that vertebroplasty could effectively reinforce the osteoporotic vertebra regardless of BMD or PMMA volume. Effectiveness of additional PMMA injection tended to decrease. For patients with BMD well lower than 50mg/ml, injection of up to 30% volume of the vertebral body is recommended. However, less than 30% is recommended otherwise to avoid any complications from excessive PMMA because the strength has already reached the normal level.

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Observation on the Clinical Sign and Blood Pictures of Thoroughbred Racehorses with Acepromazine (경주마에서의 Acepromazine Maleate 투여에 따른 임상증상과 혈액상의 변화 조사)

  • Kim Byung-Sun;Lim Young-Jae;Choi Hee-In;Park Seong-Jun
    • Journal of Veterinary Clinics
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    • v.11 no.1
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    • pp.419-426
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    • 1994
  • Acepromazine maleate(sedaject) was injected to 3 and 5 thoroughbred racehorses at 0.2mg/kg/bw, intramurculary(IM) and 0.1mg/kg/bw, intravenously(IV) respectively, and investigated the changes of clinical signs and blood pictures In before and after injection. Sedation was induced within 15 minutes after injection at the two groups and general sedation with towering of the upper eyelids and penile protrusion lasted about 7~9 hours and 9~11 hours at IV group and IM group following injection respectively. Heart beat and respiratory rates were induced within 15 minutes and then slowly returned to preinjection levels at 3 and 7 hours in the two group following injection respectively. Body temperature was decreased within IS minutes and the effect was Peaked after 30 minutes and 3 hours in IV group and IM group respectively, and then slowly returned to preinjection levels. RBC parameters, Hb and PCV were decreased about 30% at 1~5 hours and then returned to preinjection values at 13~24 hours in two groups Total WBC number were decreased slightly within 30 minutes and then returned to preinjection level at 13~24 hours in IV group but being still decreased 24 hours after dosing in IM group serum glucose level, SGOT and ALP activity were not changed significantly. Generally the set of sedation and awakening signs were faster in IV group than in IM group and RBC parameters and total WBC were depressed markedly in IM group than in IV group.

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Voltage Controlled Injection-Locked Oscillator Design at 2.4 GHz Band for Wideband Applications (광대역 응용을 위한 2.4 GHz 대역 전압 제어 주입 동기 발진기 설계)

  • Yoon, Won-Sang;Lee, Hun-Sung;Lee, Hee-Jong;Pyo, Seong-Min;Kim, Young-Sik;Han, Sang-Min
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.22 no.3
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    • pp.292-298
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    • 2011
  • In this paper, a voltage controlled injection-locked oscillator(VC-ILO) is proposed for wideband applications. From the control of the free-running frequency by a varactor diode, the wide frequency locking range can be obtained for low-level injected signals. The proposed VC-ILO is implemented on an FR-4 substrate with a thickness of 0.8 mm. The free-running frequencies of the oscillator is 2.39~2.52 GHz at the control voltage of 0~5 V. While the frequency locking range of over 50 MHz is presented for -10 dBm injected signal level at a fixed frequency, the locking range of over 90 MHz can be achieved for -30 dBm by controlling the free-running frequency.

Sensitivity Analysis of Artificial Recharge in Consideration of Hydrogeologic Characteristics of Facility Agricultural Complex in Korea : Hydraulic Conductivity and Separation Distance from Injection Well to Pumping Well (국내 시설농업단지의 수리지질 특성을 고려한 인공함양 민감도 분석 : 수리전도도 및 주입정과 양수정의 이격거리)

  • Choi, Jung Chan;Kang, Dong-hwan
    • Journal of Environmental Science International
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    • v.28 no.9
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    • pp.737-749
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    • 2019
  • In this study, the sensitivity analysis of hydraulic conductivity and separation distance (distance between injection well and pumping well) was analyzed by establishing a conceptual model considering the hydrogeologic characteristics of facility agricultural complex in Korea. In the conceptual model, natural characteristics (topography and geology, precipitation, hydraulic conductivity, etc.) and artificial characteristics (separation distance from injection well to pumping well, injection rate and pumping rate, etc.) is entered, and sensitivity analysis was performed 12 scenarios using a combination of hydraulic conductivity ($10^{-1}cm/sec$, $10^{-2}cm/sec$, $10^{-3}cm/sec$, $10^{-4}cm/sec$) and separation distance (10 m, 50 m, 100 m). Groundwater drawdown at the monitoring well was increased as the hydraulic conductivity decreased and the separation distance increased. From the regression analysis of groundwater drawdown as a hydraulic conductivity at the same separation distance, it was found that the groundwater level fluctuation of artificial recharge aquifer was dominantly influenced by hydraulic conductivity. In the condition that the hydraulic conductivity of artificial recharge aquifer was $10^{-2}cm/sec$ or more, the radius of influence of groundwater level was within 20 m, but In the condition that the hydraulic conductivity is $10^{-3}cm/sec$ or less, it is confirmed that the radius of influence of groundwater increases sharply as the separation distance increases.

Muscle Weakness after Repeated Injection of Botulinum Toxin Type A Evaluated by Dental Prescale (덴탈 프리스케일로 평가한 보툴리눔 A형 독소 반복 주사 후 근육의 약화)

  • Byun, Young-Sub;Song, Ji-Hee;Choi, Young-Chan;Kim, Seong-Taek
    • Journal of Dental Rehabilitation and Applied Science
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    • v.26 no.1
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    • pp.69-75
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    • 2010
  • Botulinum toxin type A(BTX-A) has been applied successfully to treat masseteric hypertrophy. However it can cause muscle weakness. This study was designed to measure the change in maximum bite force(MBF) after BTX-A injection into human masseter muscle and to evaluate the influence of a booster(repeated) injection. Thirty volunteers completed 18-week follow-up and MBF was measured. At 18 weeks after the first injection, a booster injection was given to 14 patients and they were followed up until 18 weeks from the booster injection. The mean MBF was approximately 20% lower at 2 weeks than before the injection, and it recovered gradually after 4 weeks to return to the preinjection level at 12 weeks. The MBF differed significantly between before the injection and at 2, 4, and 8 weeks after the injection(p<0.05). In booster injection group(n=14),the MBF decreased markedly at 6 weeks(p<0.05),and it recovered gradually in 12 weeks. The MBF was significantly reduced after booster injection of BTX-A into the human masseter muscle. The degree of discomfort experienced by the subjects had little effect on normal mastication.

Advanced Static Over-modulation Scheme using Offset Voltages Injection for Simple Implementation and Less Harmonics

  • Lee, Dong-Myung
    • Journal of Electrical Engineering and Technology
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    • v.10 no.1
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    • pp.138-145
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    • 2015
  • In this paper, a novel static overmodulation scheme (OVM) for space-vector PWM (SVPWM) is proposed. The proposed static OVM scheme uses the concept of adding offset voltages in linear region as well as overmodulation region to fully utilize DC-link voltage. By employing zero sequence voltage injection, the proposed scheme reduces procedures for achieving SVPWM such as complicated gating time calculation. In addition, this paper proposes a stepwise discontinuous angle movement in high modulation region in order to reduce Total Harmonic Distortion (THD). The validity of the proposed scheme is verified through theoretical analysis and experimental results.