• Title/Summary/Keyword: Initial growth

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Growth and Fermentation Characteristics of Saccharomyces cerevisiae NK28 Isolated from Kiwi Fruit

  • Lee, Jong-Sub;Park, Eun-Hee;Kim, Jung-Wan;Yeo, Soo-Hwan;Kim, Myoung-Dong
    • Journal of Microbiology and Biotechnology
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    • v.23 no.9
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    • pp.1253-1259
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    • 2013
  • The influences of glucose concentration, initial medium acidity (pH), and temperature on the growth and ethanol production of Saccharomyces cerevisiae NK28, which was isolated from kiwi fruit, were examined in shake flask cultures. The optimal glucose concentration, initial medium pH, and temperature for ethanol production were 200 g/l, pH 6.0, and $35^{\circ}C$, respectively. Under this growth condition, S. cerevisiae NK28 produced $98.9{\pm}5.67$ g/l ethanol in 24 h with a volumetric ethanol production rate of $4.12{\pm}0.24g/l{\cdot}h$. S. cerevisiae NK28 was more tolerant to heat and ethanol than laboratory strain S. cerevisiae BY4742, and its tolerance to ethanol and fermentation inhibitors was comparable to that of an ethanologen, S. cerevisiae D5A.

Aquatic Plants for Wastewater Treatment (수생식물을 이용한 수질정화에 관한 연구)

  • 나규환;권성환;이장훈
    • Journal of Environmental Health Sciences
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    • v.22 no.3
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    • pp.49-55
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    • 1996
  • Water parsley(Oenanthe javanica(Blume) DC) was raised with varying population density(S) in the laboratory aquarium unit to determine the growth equation. The population density was measure after 7 days. The resultant growth curve was well fit to the equation 1/S = A+B (1/S0) with a high correlation coefficient ($R^2$ = 0.999). The maximum specific absorption rate was $9.011 \times 10^{-5}$ kg $NO_x-N/kg$ water parsley$\cdot$day and $1.31 \times 10^{-5}$ kg $PO_4-P/kg$ water parsley$\cdot$day when the average population density was $2.62 kg/m^2$. The relationship between population density and nutrient absorption rate, the absorption rate of $NO_x-N$ was 5.04~5.24 mg/l$\cdot$day when the population density was $7.51~10.0 $mg/m^2\cdot day$ and the absorption rate of $PO_4-P$ was 0.56~0.78 mg/l$\cdot$day when the population density was 5.02~10.0 $kg/m^2\cdot day$. Taking into account the nutrient absorption rate and growth rate, the population density between $7.0 kg/m^2\cdot day$ and $8.0 kg/m^2 \cdot day$ was selected. The removal rate of nutrient was investigated after 7 days culture. Removal rate of $NO_x-N$ was 95.6~99.95% with initial concentration of 35 mg $NO_x-N/l$, and the removal rate of $PO_4-P$ was also high, indicating 80.24~98.9% with initial concentration of 5.95 mg $PO_x-P/l$.

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PWSCC Crack Growth Analysis Using Numerical Method in the Inner Surface Repair Weld of A Nozzle (노즐 이종금속용접부의 내면 보수용접부에서 수치해석법을 이용한 PWSCC 균열성장해석)

  • Kim, Sang-Chul;Kim, Mann-Won
    • Journal of Welding and Joining
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    • v.29 no.2
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    • pp.64-71
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    • 2011
  • In this paper, crack propagation analyses in the inner diameter (ID) repair weld of the dissimilar metal weldment of a nozzle were performed using a finite element alternating method (FEAM). To calculate the theoretical solution for the crack tip stress intensity factor, a weak type singular integral equation consisted of crack surface traction and dislocation density function was constructed and solved in conjunction with the FEAM. A two-dimensional axisymmetric finite element nozzle model was prepared and ID repair welding was simulated. An initial crack, 10% depth of weld thickness, was assumed and crack propagation trajectory from the initial crack to the 75% depth of thickness was calculated using the FEAM. Crack growth versus time curve was also calculated and compared with the curves obtained from ASME code method. With the method constructed in this paper, crack propagation trajectory and crack growth time were calculated automatically and effectively.

Nocardia orientalis 변이주에 의한 고농도 혼합당을 이용한 반코마이신 생산

  • 김창호;고영환;고중환
    • Microbiology and Biotechnology Letters
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    • v.24 no.4
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    • pp.404-407
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    • 1996
  • The effects of carbon sources on vancomycin production were investigated using Nocardia orientalis CSVC 3300. Among carbon sources tested, glucose, maltose and fructose were effective for the production of vancomycin. Glucose was favored for growth, but decrease the production of vancomycin at the concentration above 7.5%. In comparison, maltose did not decrease the production of vancomycin up to the concentration of 20%. When the mixture of glucose and maltose was used in the ratio 1:3 to 1:4, the highest production of vancomycin was achieved. When glucose concentration was set at 3.0%, catabolite repression could not be observed up to total sugar concentration of 16.0%. Fermentation was carried out using commercial hydrolyzed starch composed of glucose, maltose, maltotriose and maltotetraose, The initial glucose concentration was set at 3.0% and subsequent oligosaccharide consumption was monitored by checking their supernatant with HPLC. During initial cultivation for 38 hour, glucose was the sole carbon source leading to rapid growth. After cell growth stopped, the maltose and glucose concentrations increased due to degradation of maltotriose and maltotetraose, but glucose level was maintained at around 3.0%. After 70 hour fermentation, maltose slowly converted to glucose, and vancomycin production continued during the period.

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Ethanol Production from Xylulose by Saccharomyces cerevisiae (효모에의한 Xylulose로부터의 에탄올 생산)

  • 안동군;이광근서진호
    • KSBB Journal
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    • v.7 no.1
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    • pp.27-31
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    • 1992
  • Xylose represents a major component of cellulosic materials. This paper describes patterns of ethanol fermentation by Saccharomyces cerevisiae from xylulose, which is an isomer of xylose. Special emphasis was placed on the effects of xylulose concentration and growth temperature on cell growth and ethanol yield. The maximum specific growth of $0.087 1/hr^{-1}$ was obtained at an initial xylulose concentration of 5 g/1. The ethanol yield was propotional to initial xylulose concentrations. A xylulose concentration of 16 g/l resulted in the maximum ethanol yield of 0.49 g EtOH/g xylulose, which corresponds to 90% of a theoretical value. It is interesting to nota that xylulose metabolism was accelerated by the presence of glucose as a carbon source.

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Effect of the Yea-Sacc yeast culture on growth performance, nutrient digestibility and fecal score in weanling pigs

  • Li, Yanjiao;Li, Tianshui;Kim, Inho
    • Korean Journal of Agricultural Science
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    • v.46 no.2
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    • pp.229-237
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    • 2019
  • The influence of dietary supplementation with a yeast culture on growth performance, fecal score and nutrient digestibility was evaluated in weaned pigs in a 6-week feeding trial. A total of 50 weaning pigs with an initial average body weight (BW) of $7.46{\pm}1.60kg$ were randomly allotted into 1 of 2 dietary treatments according to the initial BW. There were 5 replicate pens in each treatment with 5 pigs per pen. The dietary treatments were as follows: 1) control, basal diet (CON) and 2) 0.10% yeast culture, basal diet supplemented with 0.1% yeast culture (YC). The average daily feed intake was significantly improved with the dietary supplementation of the yeast culture compared with the control during phases 1 and 3. Overall (0 to 6 weeks), dietary supplementation with the 0.1% yeast culture had a significant effect on the feed conversion ratio (FCR). There was no significant difference in the fecal score between the CON and YC dietary treatments. In addition, no difference in the apparent total tract digestibility was observed between the CON and YC dietary treatments. Collectively, the results of this study indicate that dietary supplementation of 0.1% yeast only improved the feed intake of weaning pigs; however, yeast culture supplementation did not affect the average daily gain, feed efficiency, total tract digestibility of dry matter, and nitrogen and energy levels as well as the fecal scores.

Analysis of Korea-Canada FTA's export effect (한-캐나다 FTA 수출효과 분석)

  • Kyung-Yun Yeom;Ki-Baeg Park
    • Korea Trade Review
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    • v.45 no.4
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    • pp.1-24
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    • 2020
  • This study aims to analyze the export effects of Korea-Canada FTA's tariff reduction, which was launched on January 1, 2015. First, as a groundwork, this study reviewed trade creation and trade diversion by investigating all possible cases of both initial FTA and additional FTA. The review was done by using the simple model of three countries assuming FTA means elimination of tariff. The review suggested that additional FTA doesn't have any negative impact on import country's social welfare contrary to initial FTA. In additional FTA, Trade diversion could happen between FTA partner countries, but it always increases import country's social welfare. In this sense, it can be called favorable trade diversion. Second, this study analyzed the export effects of Korea-Canada FTA using Clausing's product level model based on Canadian government import data from Korea which is not open to the public. It turned out that Korea-Canada FTA's tariff reduction boosted Canadian import growth rate from Korea. On the other hand, Canadian import growth rate from rest of the world was not negatively affected by Korea-Canada FTA's tariff reduction. Rather, it increased as Canadian import growth rate from Korea was higher. These findings implied trade creation rather than trade diversion of Korea-Canada FTA when it comes to Korea's export to Canada.

Influence of dietary nonstructural carbohydrate concentration on growth performance and carcass characteristics of Holstein steers

  • Ramos-Avina, Daniel;Plascencia, Alejandro;Zinn, Richard
    • Asian-Australasian Journal of Animal Sciences
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    • v.31 no.6
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    • pp.859-863
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    • 2018
  • Objective: Since very little information exists about the topic; in this experiment we compare, in a long-term finishing program, the growth-performance responses and carcass characteristics of Holstein steers where non-structural carbohydrate concentration of the diet is reduced from 64% to 51% (dry matter basis). Methods: Sixty Holstein steer calves ($129{\pm}2.2kg$) were blocked by initial weight into five groups and randomly assigned within weight groupings to 10 pens. Calves were fed with a steam-flaked corn-based finishing diets containing 51% higher fiber (HF) or 64% lower fiber (LF) nonstructural carbohydrates. Non-structural carbohydrates concentrations were manipulated substituting dried distiller grain with solubles and alfalfa hay for flaked corn. Cattle were weighed every 112 days and at the end of the experiment (day 308) when the cattle were harvested and carcass characteristics were evaluated. Results: Steers fed the HF diet showed improvement (8.8%) in average daily gain (ADG) during the initial 112-d period. This effect was followed by a numerical trend for greater ADG throughout the remainder of the study so that overall ADG tended to be greater (4.9%, p = 0.06) for the HF than for LF. There were no treatment effects on dry matter intake. Gain efficiency and estimated dietary net energy (NE) were greater 8.3% and 5.2%, respectively for HF during the initial 112-d period. Overall (308-d) gain efficiency and estimated dietary NE were similar for both dietary treatments. However, due to differences in tabular dietary NE, the ratio of observed:expected dietary NE tended to be greater (4.1%, p = 0.06) for the HF vs LF diet. There were no treatment effects on carcass characteristics except for a tendency toward a slightly greater (0.5%, p = 0.09) estimated carcass yield. Conclusion: Reducing the non-structural carbohydrate concentration of a conventional steam-flaked corn-based growing finishing diet for Holstein steers can effectively enhance growth performance, particularly during the early growing and late finishing phases.

Studies on Cultural Characteristics for High Density Fermentation of Phellinus linteus WI-001 (Phellinus linteus WI-001 균사체의 고밀도 배양을 위한 배양학적 특성 연구)

  • 김종래;권호균;전계택;김규중;이계관
    • Microbiology and Biotechnology Letters
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    • v.28 no.2
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    • pp.105-110
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    • 2000
  • Various environmental factors such as pH, temperature and initial glucose concentration were investigated for enhancing cell growth in fermentations of Phellinus linteus WI-OOl, a producer of polysaccarides with potent anticancer activities. Optimal pH and temperature were around 5.5 and $28^{\circ}C$, respectively. Relatively little variation of pH was observed ranging between 5.5 and 6.5 during the whole fermentation period. Maximum cell concentration and specific growth rate were investigated in the media containing initial glucose concentrations of 0.5%, 1 %, 2%, 3% and 4%. High initial glucose concentration enhanced biomass production but showed negative effect on specific growth rate. In bioreactor experiments with various feeding strategies, increases of 28% and 42% in final cell concentration were obtaind as compared to conventional batch process, by adopting pulse and continuous supplement of 2% glucose solution, respectively.

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The Effect of Achievement Goal Orientation on College Life Satisfaction and Sense of Belongings: The Longitudinal Analysis with Latent Growth Modeling (성취목표지향성이 대학생활만족도와 소속감에 미치는 영향: 잠재성장모형을 이용한 종단 분석)

  • Kim, Yong Suk
    • Journal of Practical Engineering Education
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    • v.11 no.2
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    • pp.291-303
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    • 2019
  • The purpose of this research was to investigate the effect of the achievement goal orientation on college students' life satisfaction and sense of the belonging. This study used the analysis of the latent growth model to comprehend the slope of the 3 years longitudinal data. The result was that mastery approach and mastery avoidance affected the initial value of college life satisfaction, but not its slope as the time of 3 years went. On the other hand, performance approach was the opposite of mastery approach and mastery avoidance to college students' life satisfaction. Performance avoidance did not have an influence on both the initial value and the slope of college students' life satisfaction. The effect of the achievement goal orientation to the sense of belonging was also explored and its result was that mastery approach and mastery avoidance affected the initial value of the sense of belonging. However, it was different that performance approach did not influence the slope of the sense of belonging.