• Title/Summary/Keyword: Initial growth

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Cellular Automaton Models Revealing Effects of Initial Bacterial Distribution on Biofilm Growth (생물막 성장에 대한 세균의 초기 분포영향을 나타내는 셀룰라오토마톤 모델)

  • Lee, Sang-Hee;Choi, Kyung-Hee;Chon, Tae-Soo
    • Korean Journal of Ecology and Environment
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    • v.37 no.3 s.108
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    • pp.297-303
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    • 2004
  • Two dimensional cellular automaton (CA) models were developed to investigate growth of biofilms in aquatic ecosystems. Simple local rules on CA were applied to governing growth of bacterial populations in relation to different nutrient concentrations. Initial bacterial distribution played an important role in determining population size and morphology of biofilm at low concentrations of nutrition. With clumped distribution, population size increased slowly compared with uniform and random distributions, while the porosity tented to be higher with uniform distribution compared with other initial distributions.

Studies on the Brewing of Apple Wine -Culture Conditions of a Cider Yeast, Saccharomyces sp. R-11 on the Synthetic Medium (사과주(酒) 양조(釀造)에 관한 연구(硏究) -사과주효모(酒酵母) Saccharomyces sp. R-11의 합성배지((合成培地)에서의 배양(培養) 조건(條件)-)

  • Chung, Ki-Taek;Lee, Jong-Soo
    • The Korean Journal of Mycology
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    • v.10 no.2
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    • pp.75-83
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    • 1982
  • As a primary study for cell growth and alcohol production of a cider yeast, Saccharomyces sp. R-11, cultural and nutritional characteristics of the strain were investigated. The results obtained were as follows: The optimum culture medium for this strain was a synthetic medium, Henneberg B, and sucrose was the best carbon source for yeast growth and alcohol production. Optimum sugar concentrations for yeast growth and alcohol production were 15% and 25%, respectively. Optimum pH and temperature of the basal medium for growth of this strain were 4.5 and $30^{\circ}C$ respectively. The yeast growth was enhanced by the addition of 100 ppm of $Mg^{2+}$, but significantly inhibited by the addition of 100 ppm of $Co^{2+}$. Lower temperature and maintenance of optimum pH for yeast growth increased the final alcohol concentration. Under optimum condition for cell growth at stationary culture, generation time and specific growth rate of the strain were 7.5 hr and 0.092 $hr^{-1}$, respectively. At 8% initial alcohol concentration, yeast growth was inhibited about 50% and this strain could not be grown at more than 12% initial alcohol. The strain could be grown at less than 125ppm $SO_2$without alcohol addition, and at less than 75 ppm $SO_2$ with 8% initial alcohol. The higher sulfur dioxide concentration of a medium, the longer lag phase in yeast growth was observed. This strain could induced alcoholic fermentation at less than 10% initial alcohol concentration with 0 and 25 ppm $SO_2$, at less than 8% initial alcohol with 50 and 75 ppm $SO_2$, and at less than 6% initial alcohol with 100 and 125 ppm $SO_2$.

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A Study on the Mathematical Model of Cell Growth by Carrot Cell Suspension Culture (당근세포의 현탁배양에 의한 세포성장 모델연구)

  • 채보희;허병기
    • KSBB Journal
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    • v.4 no.3
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    • pp.259-265
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    • 1989
  • In a batch fermentation process using carrot cell suspension culture, the effect of initial concentration of limiting nutrients(glucose and phosphate) on the specific growth rate and cell yield was investigated. The period of exponential growth is about 2 days and the consumption of glucose and phosphate in culture medium was very small when the initial concentrations of glucose and phosphate are 1.49g/1 ~ 3.01g/l and 0.08 ~ 0.32mM respectively. The specific growth rate of cells ranged from TEX>$0.15\;day^{-1}$ to $0.3\;day^{-1}$ irregularly. And the ratio of the initial concentration of glucose to phosphate did not affect the specific growth rate and the cell yield. The increase on cells had linear relationship with the consumption of limiting nutrients. Therefore, the increase of cells was found to be more influenced by the concentration of glucose than that of phosphate.

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The effect of LED light wavelength on the growth of fingerling Sebastes inermis (볼락(Sebastes inermis)치어의 성장에 미치는 LED 광파장의 영향)

  • Shin, Hyeon-Ok;Heo, Min-A;Heo, Gyeom
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.51 no.2
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    • pp.179-187
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    • 2015
  • Experiments designed to measure the effect of LED (light-emitting diode) light wavelength on the growth of fingerling rockfishes (Sebastes inermis) were conducted. Fingerling rockfishes (average body weight of individual: 1.13g) were divided into two groups by wavelength of the LED light [light power: 1,620 mW; wavelength: 518 nm (green color), 622 nm (red color)]. Triplicate groups of 180 individuals were reared over 7 weeks. Lighting duration was 14 hours from 06:00 to 20:00. A water tank exposed on the natural light in a room through the windows was used as a control. At results of the first experiment [initial average body weight (BW) of individual: 1.13 g; standard deviation (SD): 0.13 g], the final individual BW exposed on the green color was increased 0.39 g than the red color, and decreased 0.12 g than the natural light in the room. At results of the second (initial individual BW: 5.07 g; SD: 0.70 g) and the third experiment (initial individual BW: 10.67 g; SD: 0.67 g), the final individual BW exposed on the green color was increased 1.07 g and 2.55 g than the red color, respectively, and increased 0.57 g and 0.84 g than the natural light, respectively. The relative growth rate of the green color was higher about 8% significantly (p<0.05) than the natural light. In the case of the red color the relative growth rate was lower significantly (p<0.05) than the natural light.

New Evaluation of Initial Growth Mechanisms of Hydroxyapatite on Self-assembled Collagen Nanofibrils by Using ToF-SIMS and AFM Techniques

  • Park, Young-Jae;Choi, Gyu-Jin;Lee, Tae-Geol;Lee, Won-Jong;Moon, Dae-Won
    • Proceedings of the Korean Vacuum Society Conference
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    • 2010.02a
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    • pp.397-397
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    • 2010
  • Bone is considered as hierarchically organized biocomposites of organic (collagen) and inorganic (hydroxyapatite) materials. The precise structural dependence between hydroxyapatite (HAp, $Ca_{10}(PO_4)_6(OH)_2)$ crystals and collagen fibril is critical to unique characteristics of bone. To meet those conditions and obtain optimal properties, it is essential to understand and control the initial growth mechanisms of hydroxyapatite at the molecular level, such as other nano-structured materials. In this study, collagen fibrils were prepared by adsorbing native type I collagen molecules onto hydrophobic surface. Hydrophobicity was introduced on the Si wafer surface by using PECVD (plasma enhanced chemical vapor deposition) method and cyclohexane as a precursor. Biomimetic nucleation and growth of HAp on the self-assembled collagen nanofibrils were occurred through incubation of the sample in SBF (simulated body fluid). Chemical and morphological evolution of HAp nanocrystals was investigated by surface-sensitive analytical techniques such as ToF-SIMS (Time-of-Flight Secondary Ion Mass Spectrometry) and AFM (Atomic Force Microscopy) in the early growth stages (< 24 hrs). The very initial stages (< 12 hrs) of mineralization could be clearly demonstrated by ToF-SIMS chemical mapping of surface. In addition to ToF-SIMS and AFM measurement, scanning electron microscopy, energy dispersive spectroscopy and X-ray diffraction analysis were conducted to characterize the HAp layer in the late stages. This study is of great importance in the growth of real bone-like materials with a structure analogous to that of natural bones and the development of biomimetic nanomaterials.

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Detection of Lignans from Transformed Root Cultures of Schisandra chinensis Baillon (오미자의 형질전환된 근으로부터 리그난 화합물의 검출)

  • Hwang, Sung-Jin;Pyo, Byoung-Sik;Hwang, Baik
    • Korean Journal of Medicinal Crop Science
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    • v.12 no.6
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    • pp.448-453
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    • 2004
  • Transformed roots of Schisandra chinensis were obtained following co-cultivation of in vitro cultivated plantlet segments with Agrobaterium rhizogens ATCC15834. This root was examined for its growth and gomisin J contents under various culture conditions. Among the six basal culture media tested, WPM (Lloyd & McCown, 1980) medium supplemented with 5% sucrose was the best roots growth 6.2 (g D.W/flask) and gomisin J accumulation 1.56 $(X10^{-3}\;ug/g\;D.W)$. Initial inoculum size correlated with the yield of biomass while gomisin J contents was not affect. Gomisin J production was influenced by the initial sucrose concentration and the highest production yield was achieved at the concentration of 7%. The optimal shaking speeds for roots growth and gomisin J production was 120 and 140 rpm, respectively.

Biodegradation and Kinetics of Trichloroethylene by Micrococcus sp. MS-64K (Micrococcus sp. MS-64K에 의한 Trichloroethylene의 분해특성 및 Kinetics)

  • 김종수;박근태
    • Journal of Environmental Science International
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    • v.6 no.5
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    • pp.481-488
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    • 1997
  • Microorganisms capable of degrading trlchloroethylene(TCEI using phenol as a induction substrate were isolated from industrial effluents and soil. The strain MS-64K which had the highest blodegradablllty was identified as the genus Micrococcus. The optimal conditions of medium for the growth and blodegadatlon of trlchloroethylene were observed as follows; the initial pH 7.0, trlchloroethylene 1, 000ppm as the carbon source, 0.2% ${(NH_4)}_2SO_4$, as the nitrogen source. respectively. Lag period and degradation time on optimal medium were shorter than those on Isolation medium. Growth on the optimal medium was Increased. Addition of 0.1% Triton X-100 Increased the growth rate of Micrococcus sp. MS-64K, but degradation was equal to optimal medium. Trlchloroethylene degradation by Micrococcus sp. MS-64K was shown to fit logarithmic model when the compound was added at initial concentration of 1, 000ppm.

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Development of Indicators for Evaluating the Size of Economic Investment for Child Care in Korea (한국아동보육사업의 경제적 규모 평가를 위한 지표개발 연구)

  • Lee, Seung-Hee;Paik, Il-Woo;Kim, Myoung-Soon
    • Korean Journal of Child Studies
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    • v.26 no.1
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    • pp.183-202
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    • 2005
  • This study performed intensive indicators based on a model of economics of education. Trends of childcare in Korea were obtained by producing values developed with statistical data. Results showed that such simple initial indicator values as numbers of children, institutions and teachers have improved. However, intensive indicator values that take demographic and economic conditions into consideration have not reached the same rate of progress as the initial indicator values. In other words, qualitative growth remains at an unsatisfactory level in comparison to quantitative growth and to qualitative growth in members of the Organization for Economics Cooperation and Development (OECD).Thus, financial investment by the government should be expanded in order to reach the desired level of high quality in daycare for children.

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The Effect of Initial DC Bias Voltage on Highly Oriented Diamond Film Growth on Silicon

  • Dae Hwan Kang;Seok Hong Min;Ki Bum Kim
    • The Korean Journal of Ceramics
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    • v.3 no.1
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    • pp.13-17
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    • 1997
  • It is identified that the diamond films grown o bias-treated (100) silicon showed different surface morphologies and film textures according to the initial applied dc bias voltage at the same growth condition. The highly oriented diamond film (HODF) was successfully grown on -200 V bias-treated silicon substrate in which the heteroepitaxial relation of $(100)_{dimond}//(100)_{si}\; and\; [110]_{diamond}//[110]_{si}$ was identified. On the contrary, the heteroepitaxial relation was considerably disturbed in the samples bias-voltage was a key factor in growing the highly oriented diamond film on (100) silicon substrate. Considering the experimental results, we proposed a new model about heteroepitaxial diamond growth on silicon, in which 9 diamond unit cell are matched with 4 silicon cells and the bond covalency of both atoms is satisfied via the intermediate layer at the interface as well.

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Behavior of Initial Formation of Iron Nitride on Carbon Steel at Low Pressure Gas Nitriding (저압가스질화에서 탄소강의 초기 화합물층 형성 거동)

  • Kim, Yoon-Kee;Kim, Sang-Gweon
    • Journal of the Korean institute of surface engineering
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    • v.44 no.3
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    • pp.75-81
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    • 2011
  • Growth behaviors of iron-nitride on S45C steels at low pressure gas nitriding were examined. Surfaces of the steels covered with fine and porous oxide during the pre-oxidation using $N_2O$ gas. Well faceted particles connected with them were observed after 1 min nitriding. They grew steadily and filled inter-pores during additional nitriding process. From the X-ray diffraction analysis, ${\gamma}'$-iron nitride was dominantly formed at the initial stage but the amount of ${\varepsilon}$-iron nitride was rapidly increased as nitriding treatment time. The porous layer was formed on the particles and thickened up to half of nitride layer after 60 min nitriding. The observed growth behaviors were discussed in internal stress related with volume expansion involved in transforming from iron to iron-nitrides.