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Constructing a Three-Dimensional Endothelial Cell Layer in a Circular PDMS Microchannel

  • Choi, Jong Seob;Piao, Yunxian;Kim, Kyung Hoon;Seo, Tae Seok
    • Proceedings of the Korean Vacuum Society Conference
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    • 2013.08a
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    • pp.274.2-274.2
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    • 2013
  • We described a simple and efficient fabrication method for generating microfluidic channels with a circular-cross sectional geometry by exploiting the reflow phenomenon of a thick positive photoresist. Initial rectangular shaped positive photoresist micropatterns on a silicon wafer, which were fabricated by a conventional photolithography process, were converted into a half-circular shape by tuning the temperature to around $105^{\circ}C$. Through optimization of the reflow conditions, we could obtain a perfect circular micropattern of the positive photoresist, and control the diameter in a range from 100 to 400 ${\mu}m$. The resultant convex half-circular photoresist was used as a template for fabricating a concave polydimethylsiloxane (PDMS) through a replica molding process, and a circular PDMS microchannel was produced by bonding two half-circular PDMS layers. A variety of channel dimensions and patterns can be easily prepared, including straight, S-curve, X-, Y-, and T-shapes to mimic an in vivo vascular network. To inform an endothelial cell layer, we cultured primary human umbilical vein endothelial cells (HUVECs) inside circular PDMS microchannels, and demonstrated successful cell adhesion, proliferation, and alignment along the channel.

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A Study on a Quality Characteristics of Pressure Leak Test of Process Piping for Offshore Plant (해양플랜트 프로세스 배관 Pressure Leak Test의 품질 특성에 관한 연구)

  • Park, Chang-Soo;Kim, Hyungwoo
    • Journal of the Korean Society of Industry Convergence
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    • v.21 no.6
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    • pp.429-437
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    • 2018
  • The process gas piping of the offshore plant can cause a massive explosion if the gas leakage occurs during operation. For the purpose of precaution of gas leakage accident, an air pressure test is performed on the process equipment tests using a test pump as much as the power to the piping inner side, mix 99% nitrogen gas and 1% helium gas. The purpose of the air pressure test is to check the work conformity process by handling and regulation for initial piping process, assembly, installation of module, welding, center alignment of the pipes assembling flange gasket in an unrestrained free state. In this paper, the regulation of the problematic air pressure test was analyzed and the solution criteria were established. And leakage tests of existing equipment were performed applying these solution methods. As a result, it was confirmed that there was no problem.

Deformation Behavior of 6063 Al Alloy Deformed by Shear-Drawing Method (전단-신선 가공된 6063 알루미늄 합금의 변형거동)

  • Ko, Young Gun;Lee, Byung Uk;Shin, Dong Hyuk
    • Korean Journal of Metals and Materials
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    • v.49 no.4
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    • pp.291-297
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    • 2011
  • This work investigated the microstructure and mechanical properties of 6063 Al alloy fabricated by shear-drawing (SD) technique where shear and drawing strains were combined together within a predetermined die. To find the optimum condition for sound deformation, three different dies having different inner angle and diameter of the exit channel were prepared. After single deformation of the present sample, the sound deformation took place without an abrupt failure of the sample if the inner angle would be greater than $135^{\circ}$ in this study, when the channel diameter of the SD die was reduced from 10 to 9 mm. Microstructural observation showed that the inner angle of $135^{\circ}$ was found to be more effective than that of $150^{\circ}$ in terms of the alignment of each grain to the shear direction imposed by SD method. In addition, the yield strength of the SD-deformed sample was twice higher than that of the initial counterpart while loosing ductility in tension.

Evaluation of Real-time Target Positioning Accuracy in Spinal Radiosurgery (척추방사선수술시 실시간 추적검사에 의한 병소목표점 위치변이 평가)

  • Lee, Dong Joon
    • Progress in Medical Physics
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    • v.24 no.4
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    • pp.290-294
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    • 2013
  • Stereotactic Radiosurgery require high accuracy and precision of patient positioning and target localization. We evaluate the real time positioning accuracy of isocenter using optic guided patient positioning system, ExacTrac (BrainLab, Germany), during spinal radiosurgery procedure. The system is based on real time detect multiple body markers attached on the selected patient skin landmarks. And a custom designed patient positioning verification tool (PPVT) was used to check the patient alignment and correct the patient repositioning before radiosurgery. In this study, We investigate the selected 8 metastatic spinal tumor cases. All type of tumors commonly closed to thoracic spinal code. To evaluate the isocenter positioning, real time patient alignment and positioning monitoring was carried out for comparing the current 3-dimensional position of markers with those of an initial reference positions. For a selected patient case, we have check the isocenter positioning per every 20 millisecond for 45 seconds during spinal radiosurgery. In this study, real time average isocenter positioning translation were $0.07{\pm}0.17$ mm, $0.11{\pm}0.18$ mm, $0.13{\pm}0.26$ mm, and $0.20{\pm}0.37$ mm in the x (lateral), y (longitudinal), z (vertical) directions and mean spatial error, respectively. And body rotations were $0.14{\pm}0.07^{\circ}$, $0.11{\pm}0.07^{\circ}$, $0.03{\pm}0.04^{\circ}$ in longitudinal, lateral, table directions and mean body rotation $0.20{\pm}0.11^{\circ}$, respectively. In this study, the maximum mean deviation of real time isocenter positioning translation during spinal radiosurgery was acceptable accuracy clinically.

Growth of vertically aligned carbon nanotubes on Co-Ni alloy metal (Co-Ni 합금위에서 수직방향으로 정렬된 탄소나노튜브의 성장)

  • Lee, Cheol-Jin;Kim, Dae-Woon;Lee, Tae-Jae;Park, Jeong-Hoon;Son, Kwon-Hee;Lyu, Seung-Chul;Song, Hong-Ki;Choi, Young-Chul;Lee, Young-Hee
    • Proceedings of the KIEE Conference
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    • 1999.07d
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    • pp.1504-1507
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    • 1999
  • We have grown vertically aligned carbon nanotubes in a large area of Co-Ni codeposited Si substrates by the thermal CVD using $C_2H_2$ gas. Since the discovery of carbon nanotubes, Synthesis of carbon nanotubes for mass production has been achieved by several methods such as laser vaporization arc discharge, and pyrolysis. In particular, growth of vertically aligned nanotubes is of technological importance for applications to FED. Recently, vertically aligned carbon nanotubes have been grown on glass by PECVD Aligned carbon nanotubes can be also grown on mesoporous silica and Fe patterned porous silicon using CVD. Despite such breakthroughs in the growth, the growth mechanism of the alignment are still far from being clearly understood. Furthermore, FED has not been clearly demonstrated yet at a practical level. Here, we demonstrate that carbon nanotubes can be vertically aligned on catalyzed Si substrate when the domain density reaches a certain value. We suggest that steric hindrance between nanotubes at an initial stage of the growth forces nanotubes to align vertically and then nanotubes are further grown by the cap growth mechanism.

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Types and Yields of Carbon Nanotubes Synthesized Depending on Catalyst Pretreatment

  • Go, Jae-Seong;Lee, Nae-Seong
    • Proceedings of the Materials Research Society of Korea Conference
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    • 2011.10a
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    • pp.17.2-17.2
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    • 2011
  • Double-walled carbon nanotubes (DWCNTs) were grown with vertical alignment on a Si wafer by using catalytic thermal chemical vapor deposition. This study investigated the effect of pre-annealing time of catalyst on the types of CNTs grown on the substrate. The catalyst layer is usually evolved into discretely distributed nanoparticles during the annealing and initial growth of CNTs. The 0.5-nm-thick Fe served as a catalyst, underneath which Al was coated as a catalyst support as well as a diffusion barrier on the Si substrate. Both the catalyst and support layers were coated by using thermal evaporation. CNTs were synthesized for 10 min by flowing 60 sccm of Ar and 60 sccm of H2 as a carrier gas and 20 sccm of C2H2 as a feedstock at 95 torr and $750^{\circ}C$. In this study, the catalyst and support layers were subject to annealing for 0~420 sec. As-grown CNTs were characterized by using field emission scanning electron microscopy, high resolution transmission electron microscopy, Raman spectroscopy, and atomic force microscopy. The annealing for 90~300 sec caused the growth of DWCNTs as high as ~670 ${\mu}m$ for 10 min while below 90 sec and over 420 sec 300~830 ${\mu}m$-thick triple and multiwalled CNTs occurred, respectively. Several radial breathing mode (RBM) peaks in the Raman spectra were observed at the Raman shifts of 112~191 cm-1, implying the presence of DWCNTs, TWCNTs, MWCNTs with the tube diameters 3.4, 4.0, 6.5 nm, respectively. The maximum ratio of DWCNTs was observed to be ~85% at the annealing time of 180 sec. The Raman spectra of the as-grown DWCNTs showed low G/D peak intensity ratios, indicating their low defect concentrations. As increasing the annealing time, the catalyst layer seemed to be granulated, and then grown to particles with larger sizes but fewer numbers by Ostwald ripening.

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Automatic Segmentation of Renal Parenchyma using Graph-cuts with Shape Constraint based on Multi-probabilistic Atlas in Abdominal CT Images (복부 컴퓨터 단층촬영영상에서 다중 확률 아틀라스 기반 형상제한 그래프-컷을 사용한 신실질 자동 분할)

  • Lee, Jaeseon;Hong, Helen;Rha, Koon Ho
    • Journal of the Korea Computer Graphics Society
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    • v.22 no.4
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    • pp.11-19
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    • 2016
  • In this paper, we propose an automatic segmentation method of renal parenchyma on abdominal CT image using graph-cuts with shape constraint based on multi-probabilistic atlas. The proposed method consists of following three steps. First, to use the various shape information of renal parenchyma, multi-probabilistic atlas is generated by cortex-based similarity registration. Second, initial seeds for graph-cuts are extracted by maximum a posteriori (MAP) estimation and renal parenchyma is segmented by graph-cuts with shape constraint. Third, to reduce alignment error of probabilistic atlas and increase segmentation accuracy, registration and segmentation are iteratively performed. To evaluate the performance of proposed method, qualitative and quantitative evaluation are performed. Experimental results show that the proposed method avoids a leakage into neighbor regions with similar intensity of renal parenchyma and shows improved segmentation accuracy.

Registration between High-resolution Optical and SAR Images Using linear Features (선형정보를 이용한 고해상도 광학영상과 SAR 영상 간 기하보정)

  • Han, You-Kyung;Kim, Duk-Jin;Kim, Yong-Il
    • Korean Journal of Remote Sensing
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    • v.27 no.2
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    • pp.141-150
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    • 2011
  • Precise image-to-image registration is required to process multi-sensor data together. The purpose of this paper is to develop an algorithm that register between high-resolution optical and SAR images using linear features. As a pre-processing step, initial alignment was fulfilled using manually selected tie points to remove any dislocations caused by scale difference, rotation, and translation of images. Canny edge operator was applied to both images to extract linear features. These features were used to design a cost function that finds matching points based on their similarity. Outliers having larger geometric differences than general matching points were eliminated. The remaining points were used to construct a new transformation model, which was combined the piecewise linear function with the global affine transformation, and applied to increase the accuracy of geometric correction.

Pathophysiology of Articular Cartilage Injury (관절 연골 손상의 병태 생리)

  • Park, Jung-Ho
    • Journal of Korean Orthopaedic Sports Medicine
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    • v.4 no.1
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    • pp.6-11
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    • 2005
  • Injury of articular cartilage can be classified into acute injury and chronic degenerative osteoarthritis Acute mechanical trauma on articular cartilage causes injuries that are divided into three distinct types based on the depth of injury: microdamage, chondral fracture, osteochondral fracture and each type has different potential of healing response and long-term prognosis. Articular cartilage undergoes degradation in response to a number of stimuli and eventually degenerative osteoarthritic changes will progress. The extent of initial injury to the articular cartilage is the most important factor affecting the long-term outcome of the healing response and other variables such as the size of lesion, site, age, activity level, obesity, limb alignment are also important factors. In this review, the pathophysiology that occurs within articular cartilage after different injuries and the effect of nonsurgical treatment mainly in physicochemical aspect and biological aspect will be discussed.

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