• Journal of the Korean Graphic Arts Communication Society
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• v.20 no.2
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• pp.31-43
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• 2002

Screen printing is a stencil process whereby ink is transferred to the substrate through a stencil supported by a fine fabric mesh. Therefore screen had a tendency to distort and swell, as ink was deposited between the fibers, and were difficult to clean. The tow importance of stencil parameters that affect print quality are stencil thread diameter and the fabric thickness because of their influence on both ink deposit and print definition. Since screen printing inks can be formulated to adhere to almost any surface, and the printing process itself can be handled almost any substrate in a wide variety of shape, screen printing is a very versatile process. The small size pronting is reproduced image used screen printing because the surface of substrates is not suited at screen printing method. In screen printing, the need of high definition printing is gradually increasing according to developing special inks. A conventional haftone, so called AM screening, is simple and easy to implement, but the haftone dot patterns by using this method are not free for the moire fringe. This paper is used densitometry and image analysis to investigate relation with printing according to screen mesh, opening size and resolution of copy in image reproduction used FM screen. We had the good result of dot gain and tone reproduction on the screen printing of high definition using FM screen.

• Journal of Animal Reproduction and Biotechnology
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• v.35 no.1
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• pp.65-72
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• 2020

Fish ovarian germline stem cells (OGSCs) that have the abilities to self-renew and differentiate into functional gametes can be used in various researches and applications. A main issue to be solved for effective utilization of fish OGSCs is the development of their stable in vitro culture condition, but only few researches about fish OGSC culture have been reported so far. In this study, in order to find the clues to develop the culture condition for OGSCs from Japanese medaka (Oryzias latipes), we tried to establish somatic cell lines as a candidate for the feeder cells and evaluated its supporting effects on the culture of ovarian cell populations from O. latipes. As the results, the somatic cell lines could be established only from the embryonic tissues among three tissues derived from embryos, fins and ovaries. Three embryonic cell lines were tested as a feeder cell for the culture of ovarian cell population and all three cell lines induced cell aggregation formation of the cultured ovarian cells whereas the feeder-free condition did not. Furthermore, a significant cellular proliferation was observed in the ovarian cells cultured on two of three cell lines. As a trial to increase the capacity of the cell lines as a feeder cell that supports the proliferation of the cultured ovarian cells, we subsequently established a stable line that expresses the foreign O. latipes fibroblast growth factor 2 (FGF2) from an embryonic cell line and evaluated its effectiveness as a feeder cell. The ovarian cells cultured on FGF2 expressing feeder cells still formed cell aggregates but did not show a significant increase in cellular proliferation compared to those cultured on non-transformed feeder cells. The results from this study will provide the fundamental information for in vitro culture of medaka OGSCs.

• Journal of Animal Reproduction and Biotechnology
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• v.39 no.2
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• pp.138-144
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• 2024

Background: As the number of households raising companion dogs increases, the pet genetic analysis market also continues to grow. However, most studies have focused on specific purposes or native breeds. This study aimed to collect genomic data through single nucleotide polymorphism (SNP) chip analysis of companion dogs in South Korea and perform genetic diversity analysis and SNP annotation. Methods: We collected samples from 95 dogs belonging to 26 breeds, including mixed breeds, in South Korea. The SNP genotypes were obtained for each sample using an Axiom^TM Canine HD Array. Quality control (QC) was performed to enhance the accuracy of the analysis. A genetic diversity analysis was performed for each SNP. Results: QC initially selected SNPs, and after excluding non-diverse ones, 621,672 SNPs were identified. Genetic diversity analysis revealed minor allele frequencies, polymorphism information content, expected heterozygosity, and observed heterozygosity values of 0.220, 0.244, 0.301, and 0.261, respectively. The SNP annotation indicated that most variations had an uncertain or minimal impact on gene function. However, approximately 16,000 non-synonymous SNPs (nsSNPs) have been found to significantly alter gene function or affect exons by changing translated amino acids. Conclusions: This study obtained data on SNP genetic diversity and functional SNPs in companion dogs raised in South Korea. The results suggest that establishing an SNP set for individual identification could enable a gene-based registration system. Furthermore, identifying and researching nsSNPs related to behavior and diseases could improve dog care and prevent abandonment.

• Journal of Animal Reproduction and Biotechnology
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• v.39 no.2
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• pp.67-80
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• 2024

Background: Post-ovulatory aging (POA) of oocytes is related to a decrease in the quality and quantity of oocytes caused by aging. Previous studies on the characteristics of POA have investigated injury to early embryonic developmental ability, but no information is available on its effects on mitochondrial fission and mitophagy-related responses. In this study, we aimed to elucidate the molecular mechanisms underlying mitochondrial fission and mitophagy in in vitro maturation (IVM) oocytes and a POA model based on RNA sequencing analysis. Methods: The POA model was obtained through an additional 24 h culture following the IVM of matured oocytes. NMN treatment was administered at a concentration of 25 μM during the oocyte culture process. We conducted MitoTracker staining and Western blot experiments to confirm changes in mitochondrial function between the IVM and POA groups. Additionally, comparative transcriptome analysis was performed to identify differentially expressed genes and associated changes in mitochondrial dynamics between porcine IVM and POA model oocytes. Results: In total, 32 common genes of apoptosis and 42 mitochondrial fission and function uniquely expressed genes were detected (≥ 1.5-fold change) in POA and porcine metaphase II oocytes, respectively. Functional analyses of mitochondrial fission, oxidative stress, mitophagy, autophagy, and cellular apoptosis were observed as the major changes in regulated biological processes for oocyte quality and maturation ability compared with the POA model. Additionally, we revealed that the activation of NAD⁺ by nicotinamide mononucleotide not only partly improved oocyte quality but also mitochondrial fission and mitophagy activation in the POA porcine model. Conclusions: In summary, our data indicate that mitochondrial fission and function play roles in controlling oxidative stress, mitophagy, and apoptosis during maturation in POA porcine oocytes. Additionally, we found that NAD⁺ biosynthesis is an important pathway that mediates the effects of DRP1-derived mitochondrial morphology, dynamic balance, and mitophagy in the POA model.

• Transactions of the Korean Society for Noise and Vibration Engineering
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• v.14 no.12
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• pp.1268-1278
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• 2004

Measurement of noise is not only to know the information of acoustic pressure but to assess human response to noise. To find human response to transportation noise through the laboratory study we have to measure and reproduce noise. The method of noise reproduction is largely divided into monaural and binaural techniques. But human fundamentally hears sound through both ears, referred as binaural hearing. Binaural signal is different from monaural signal because it includes more information of physical phenomena like acoustical reflection, diffraction and refraction. Especially head and pinna play an important role in perceiving change of signal origin. So, the amplitude of binaural signal is higher than that of monaural signal and spectrum of both signals is discriminated. Most of assessment and regulation of transportation noise are, however, based on monaural measurement techniques. The quantitative difference between monaural and binaural measurement is investigated in this study. Comparison on several transportation noisesshows defect of information in monaural measurements.

• Journal of Korea Multimedia Society
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• v.17 no.7
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• pp.778-786
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• 2014

Buyer-seller watermarking protocol is one of the copyright protection techniques which combine a cryptographic protocol used in electronic commerce with a digital wetermarking scheme aiming at proving the ownership of multimedia contents and preventing the illegal reproduction and redistribution of digital contents. In this paper, it is proposed a new watermarking scheme in an encrypted domain in an image that is based on the block-DCT framework. In order to implement watermarking scheme in a public-key cryptosystem, it is divided that frequency coefficients exist as real number into integer and decimal layer. And the decimal layer is modified integer type through integral-processing. Also, for robustness and invisibility required in watermarking scheme, it is designed a direction-adaptive watermarking scheme based on locally edge-properties of each block in an image through analyzing distribution property of the frequency coefficients in a block using JND threshold.

• The Journal of Information Systems
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• v.22 no.4
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• pp.85-115
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• 2013

Digital knowledge and information goods as experience goods have some unique characteristics such as close to zero reproduction and distribution cost, high price volatility, and low price acceptability. For the reasons, the pricing policies of digital knowledge goods are very difficult and complicate. Also, most consumers of digital goods have experienced cognitive dissonance after buying decision. The purpose of this paper is to investigate what factors affect the price acceptability level and cognitive dissonance of digital knowledge goods buyers. This paper suggest a structural model that was established by the cognitive dissonance theory and S-O-R(Stimulus-Organization-Response) model. The model is consisted of four exogenous variables and three endogenous variables. The empirical test and statistical analysis suggest following results and practical implications. The variables such as product involvement and perception of price fairness that have positive roles to price acceptability have strong influence on the all the three endogenous variables. But the variables such as sale proneness and price mavenism that have negative roles to price acceptability have little influence on the all the three endogenous variables. In the model, the payment intention was very important mediating variable between exogenous variables and two dependent variables, ie. price acceptability and cognitive dissonance. These results imply that the digital knowledge portals must have some differentiated pricing policies to the customers who have price consciousness and price mavenism. Also, they need some special promotions to whom have positive attitude to the value of digital goods.

• Journal of the Korea Convergence Society
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• v.3 no.1
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• pp.19-25
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• 2012

This paper tries to solve the problems which previous methods have such as the WCDRM(Watermark and Cryptography DRM) and the model using smart card for protecting digital contents. This study provides a contents distribution model to protect the rights of author, distributor, and user as well as user's information by using technologies such as cryptography, DRM(Digital Right Management), access control, etc. The proposed system is evaluated as the most safety model compared with previous methods because it not only solves the problems which the previous methods have, but also protects four type of risks such as use of contents which other mobile devices download, the attack on the key to decode the message, the attack on leaking the contents, and the internal attack such as an illegal reproduction.

• Journal of the Korean Institute of Telematics and Electronics S
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• v.36S no.8
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• pp.55-64
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• 1999

In this paper, new gamut-mapping algorithm (GMA) that utilizes variable anchor points (center of gravity on the luminance axis) is proposed. The proposed algorithm increases luminance range, which is reduced from conventional gamut mapping toward an anchor point. In this process, this algorithm utilizes multiple anchor points with constant slopes to both reduce a sudden color change on the gamut boundary of the printer and to maintain a uniform color change during the mapping process. Accordingly, the proposed algorithm can reproduce high quality images with low-cost color devices.

• Reproductive and Developmental Biology
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• v.34 no.4
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• pp.283-288
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• 2010

During normal early embryonic development in mammals, the global pattern of genomic DNA methylation undergoes marked. changes. The level of methylation is high in male and female gametes. Thus, we cloned the cDNA of the porcine DNA methyltransferase 1 (Dnmt1) gene to promote the efficiency of the generation of porcine clones. In this study, porcine Dnmt1 cDNA was sequenced, and Dnmt1 mRNA expression was detected by reverse transcription-polymerase reaction (RT-PCR) in porcine tissues during embryonic development. The porcine Dnmt1 cDNA sequence showed more homology with that of bovine than human, mouse, and rat. The complete sequence of porcine Dnmt1 cDNA was 4,774-bp long and consisted of an open reading frame encoding a protein of 1611 amino acids. The amino acid sequence of porcine DNMT1 showed significant homology with those of bovine (91%), human (88%), rat (76%), and mouse (75%) Dnmt1. The expression of porcine Dnmt1 mRNA was detected during porcine embryogenesis. The mRNA was detected at stages of porcine preimplantation development (1-cell, 2-cell, 4-cell, 8-cell, morula, and blastocyst stages). It was also abundantly expressed in tissues (lung, ovary, kidney and somatic cells). Further investigations are necessary to understand the complex links between methyltransferase 1 and the transcriptional activity in cloned porcine tissues.