• The Journal of Internal Korean Medicine
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• v.36 no.2
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• pp.105-121
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• 2015

Objectives: This study investigated the effects of Gagam-daewhang mangcho-tang (GDM) and its components on cerulein-induced acute pancreatitis (AP) in mice Methods: The AP mouse model was induced by intra-peritoneal injection of cerulein (50 μg/kg) at hourly intervals for 6 times. The experimental drug was administrated intraperitoneally 1 hour prior to the first injection of cerulein. Mice were sacrificed at 6 hours after the last injection of cerulein. Blood samples were taken to determine serum amylase level. The pancreas and lungs were rapidly removed for histochemical examination and myeloperoxidase (MPO) assays. Results: Administration of modified GDM significantly reduced the ratio of pancreas/body weight, level of serum amylase, neutrophil infiltration, and histological damage of the pancreas and lung. In a test of the components of GDM, the Salvia miltiorrhiza (SM) group showed a significant suppression of the severity of AP. In an experiment testing the concentration of SM, the 150 mg/kg SM group showed significant attenuation of the severity of AP. Conclusions: Modified GDM and a SM water extract could attenuate AP and AP-associated lung injury via suppression of digestive enzyme secretion and MPO activity.

• Journal of Korean Society on Water Environment
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• v.34 no.3
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• pp.308-315
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• 2018

SWMM-LID was calibrated with flow monitoring data in LHI to evaluate runoff after LID application. The flow rate in the B basin was estimated to be 0.94 and 6.15 for O/S and $D_v$, respectively. In the A and C basins, the difference between the observed and simulated data was greater than in the B basin. As a result of runoff reduction efficiency by the application of LID facilities, the change of infiltration increased from 34.6 % to 45.8 % in the entire watershed, and the runoff decreased from 58.8 % to 46.3 %. In the runoff reduction of each LID facility, rain garden E showed the highest effect with 99.9 % and bioretention B showed the lowest effect with 27.5 %. In order to evaluate the efficiency of each LID facility, a comparison is made between the pore volume (V) of the LID and the catchment area (A). The runoff showed a runoff reduction effect of about 70 % above the 0.1 volume/area (V/A) value. As a result of examining the runoff reduction with LID facilities by the LID module of SWMM, a reasonable design is possible by reflecting the appropriate LID volume to drainage area.

• Biomolecules & Therapeutics
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• v.26 no.2
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• pp.157-166
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• 2018

Acute lung injury (ALI)/acute respiratory distress syndrome (ARDS) is a common clinical syndrome of diffuse lung inflammation with high mortality rates and limited therapeutic methods. Diosmetin, an active component from Chinese herbs, has long been noticed because of its antioxidant and anti-inflammatory activities. The aim of this study was to evaluate the effects of diosmetin on LPS-induced ALI model and unveil the possible mechanisms. Our results revealed that pretreatment with diosmetin effectively alleviated lung histopathological changes, which were further evaluated by lung injury scores. Diosmetin also decreased lung wet/dry ratios, as well as total protein levels, inflammatory cell infiltration and proinflammatory cytokine (eg. $TNF-{\alpha}$, $IL-1{\beta}$ and IL-6) overproduction in bronchoalveolar lavage fluid (BALF). Additionally, increased MPO, MDA and ROS levels induced by LPS were also markly suppressed by diosmetin. Furthermore, diosmetin significantly increased the expression of Nrf2 along with its target gene HO-1 and blocked the activation of NLRP3 inflammasome in the lung tissues, which might be central to the protective effects of diosmetin. Further supporting these results, in vitro experiments also showed that diosmetin activated Nrf2 and HO-1, as well as inhibited the NLRP3 inflammasome in both RAW264.7 and A549 cells. The present study highlights the protective effects of diosmetin on LPS-induced ALI via activation of Nrf2 and inhibition of NLRP3 inflammasome, bringing up the hope of its application as a therapeutic drug towards LPS-induced ALI.

• Journal of the Korean GEO-environmental Society
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• v.17 no.12
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• pp.5-16
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• 2016

This study aims to assess the influence of rainfall patterns on shallow landslides initiation. Doing so, five typical rainfall patterns with the same cumulative amount and intensity components comprising Advanced (A1 and A2), Centralized (C), and Delayed (D1 and D2) were designed based on a historical rainstorm event in Jinbu. Mt area. Those patterns were incorporated as the hydrological conditions into the Transient Rainfall Infiltration and Grid-based Regional Slope-stability Model (TRIGRS) to assess their influences on groundwater pressure and changes in the stability of the slope. The results revealed that not only the cumulative rainfall thresholds necessary to initiate landslides, but also the rate at which the factor of safety decreases and the time required to reach the critical state, are governed by rainfall patterns. The sooner the peak rainfall intensity, the smaller the cumulative rainfall threshold, and the shorter the time until landslide occurrence. Left-skewed patterns were found to have a greater effect on landslide initiation. Specifically, among five rainfalls, pattern (A1) produced the most critical state. The severity of response was followed by patterns A2, C, D1, and D2. Our conclusion is that rainfall patterns have a significant effect on the cumulative rainfall threshold, the build-up of groundwater pressure, and the occurrence of shallow landslides.

• Journal of Digital Convergence
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• v.14 no.11
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• pp.607-618
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• 2016

In order to clarify the Gosambaeksunpibokhap-bang(GBBB)'s therapeutic possibility on atopic dermatitis (AD), influences of GBBB on the changes of various immune-related factors and histological changes in NC/Nga mice were evaluated. Experimental results are as follows. Sign of recovery from AD was observed in GBBB treated group with naked eye test. The ratio of white blood cells, neutrophil, lymphocytes, monocyte in blood were decreased to 54%, 63%, 57% and 86% respectively in the GBBB group. IL-4, IL-5, IL-13, Histamine and IgE were significantly decreased to 40%, 80%, 62%, 61% and 57% respectively in the GBBB group. H&E staining showed thickness of epidermis and dermis were decreased by GBBB and inhibited the infiltration of lymphocytes. On the basis of these results, GBBB was confirmed that the possibility as an AD treatment applied externally to the skin. In the further study, immune control mechanism of GBBB will be demonstrated through the additional molecular biological research.

• IMMUNE NETWORK
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• v.11 no.1
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• pp.68-78
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• 2011

Background: Graft-versus-host disease (GVHD) is a huddle for success of hematopoietic stem cell transplantation. In this study, effects of irradiation dose on immune kinetics of GVHD were investigated using B6 ${\rightarrow}$ BALB.B system, a mouse model for GVHD after MHC-matched allogeneic transplantation. Methods: BALB.B mice were transplanted with bone marrow and spleen cells from C57BL/6 mice after irradiation with different doses. Leukocytes residing in the peripheral blood and target organs were collected periodically from the GVHD hosts for analysis of chimerism formation and immune kinetics along the GVHD development via flow cytometry. Myeloid cells were tested for production of IL-17 via flow cytometry. Results: Pre-conditioning of BALB.B hosts with 900 cGy and 400 cGy resulted in different chimerism of leukocytes from the blood and affected survival of GVHD hosts. Profiles of leukocytes infiltrating GVHD target organs, rather than profiles of peripheral blood leukocytes (PBLs), were significantly influenced by irradiation dose. Proportions of IL-17 producing cells in the infiltrating $Gr-1^+$ or $Mac-1^+$ cells were higher in the GVHD hosts with high does irradiation than those with low dose irradiation. Conclusion: Pre-conditioning dose affected tissue infiltration of leukocytes and cytokine production by myeloid cells in the target organs.

• Proceedings of the Korean Geotechical Society Conference
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• 2008.10a
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• pp.1559-1570
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• 2008

A new grouting material named 3S is developed that can be used effectively for reinforcing cut surface of weathered rock in processing of tunneling. The new material is composed of mostly micro slag cement and general Portland cement, but the material is foundered again upto $8,000\;cm^2/g$ of specific area so that it can be easily infiltrated in to the ground. For verifying technical and engineering quality of the material several laboratory tests with specially designed test apparatus were performed including compression tests, infiltration tests and resonant column tests. It was verified that the newly developed grouting material at early age of 1 or 3 days generates 200~1500% higher compressive strength and 400~560% larger elastic modulus than those of the LW(LW-1) or micro-cement(LW-2) grouting materials in the market. In addition, the new 3S grouting material could be so easily infiltrated into the model ground in the lab tests that it produces 4 times larger grouted roots in average compared to the usual water glass type grouting material(LW-1). Thus, it can be said that the newly developed grouting material can effectively prevent inflow water into tunnel compared to LW grouting materials.

• Journal of Periodontal and Implant Science
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• v.28 no.3
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• pp.431-451
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• 1998

4 beagle dogs aged over one and half years and weighed 15 to 16 Kg were utilized in this study. Experimental dehiscent defects were made in the mandibular edentulous area after removal of lower premolar. e-PTFE membrane resorbable membrane, and PDGF-BB-loaded resorbable membrane were covered at the dehiscent defects around the dental implants respectively. Animal was sacrificed at 1, 2, 3 months respectively. Non-decalicifed specimens were made and mutiple-stained for light microscopic study. The results were as follows: 1. Dehiscent defects around the implant installed in the beagle dog were an excellent defect model for studying guided bone regeneration. 2. Fibroblasts penetrated into expanded-PTFE membrane was observed and inflammatory cell infiltration was also observed around the membrane. 3. Resorbable membrane was degradaded and resorbed at 1 month after application to the dehiscent defect. Though multinucleated giant cells were observed adjacent to the membrane, that had no reverse effect on the boe regeneration. 4. PDGF-BB-Ioaded resorbable membrane was same capability as the resorbable membrane and e-PTFE membrane in the guided bone regeneration. 5. PDGF-BB-Ioaded resorbable membrane-applied site was better than resorbable membrane-applied site in the speed and maturity of bone formation. Within the above results, it was suggested that PDGF-BB-Ioaded resorbable membrane might have same bone regeneration capacity as nonresorbable membrane in the dehisced implant of the beagle dog and potentiality to use in human subjects.

• Journal of Korea Water Resources Association
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• v.47 no.1
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• pp.49-62
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• 2014

As the Urban area grows and more land is developed both within the city and in surrounding areas, hydrologic functions of the natural water cycle are altered. Urbanization creates impervious areas that negatively impact stormwater runoff characteristics. these changes to the natural hydrologic cycle result in the increased flooding, decreased groundwater recharge, increased urban heat island effects. Finally, the land use and other activities result in accumulation and washoff of pollutants from surface, resulting in water quality degradation. Therefore, in this study, evaluating and quantitative analysis of the percolation effect through infiltration experiment of permeable pavement, which is one of the ways that can reduce the problem of the dry stream. Also the SWMM model is used to study the effect of the hydrologic cycle for permeable pavement block contribution.

• Journal of Society of Preventive Korean Medicine
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• v.18 no.3
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• pp.69-80
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• 2014

Objective : Allergic rhinitis (AR) is an allergic inflammation of the nasal airways. Socheongryong-Tang traditionally has been used in Korea for treatment of allergy diseases. In the present study, we investigated whether SCRT suppresses the progression of AR in vivo and in vitro. Method : In this study, we investigated the effect of oral administration of SCRT on the Ovalbumin(OVA)-induced allergic rhinitis mouse models. Additionally, to find a possible explanation for the anti-inflammation effects of SCRT, we evaluated the inflammatory cytokine levels in LPS-stimulated mouse inflammation cell (RAW264.7) and mouse splenocyte. Results : The finding of this study demonstrated that SCRT reduced mast cells and inflammation cells infiltration in OVA-induced nasal cavity. Additionally, SCRT inhibited the production of inflammatory cytokines in splenocyte. However SCRT don't inhibited the production of inflammatory cytokines in RAW264.7 cells. Conclusion : Taken together, our results showed that oral administration of SCRT beneficial effects in allergic rhinitis, suggesting that SCRT might be a useful candidate for the treatment of allergic rhinitis.