• 한국신재생에너지학회:학술대회논문집
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• 한국신재생에너지학회 2005년도 제17회 워크샵 및 추계학술대회
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• pp.561-566
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• 2005

Polycrystalline silicon films were deposited using hot wire CVD (HWCVD). The deposition of silicon thin films was approached by the theory of charged clusters (TCC). The TCC states that thin films grow by self-assembly of charged clusters or nanoparticles that have nucleated in the gas phase during the normal thin film process. Negatively charged clusters of a few nanometer in size were captured on a transmission electron microscopy (TEM) grid and observed by TEM. The negatively charged clusters are believed to have been generated by ion-induced nucleation on negative ions, which are produced by negative surface ionization on a tungsten hot wire. The electric current on the substrate carried by the negatively charged clusters during deposition was measured to be approximately $-2{\mu}A/cm^2$. Silicon thin films were deposited at different $SiH_4$ and $H_2$ gas mixtures and filament temperatures. The crystalline volume fraction, grain size and the growth rate of the films were measured by Raman spectroscopy, X-ray diffraction and scanning electron microscopy. The deposit ion behavior of the si1icon thin films was related to properties of the charged clusters, which were in turn controlled by the process conditions. In order to verify the effect of the charged clusters on the growth behavior, three different electric biases of -200 V, 0 V and +25 V were applied to the substrate during the process, The deposition rate at an applied bias of +25 V was greater than that at 0 V and -200 V, which means that the si1icon film deposition was the result of the deposit ion of charged clusters generated in the gas phase. The working pressures had a large effect on the growth rate dependency on the bias appled to the substrate, which indicates that pressure affects the charging ratio of neutral to negatively charged clusters. These results suggest that polycrystalline silicon thin films with high crystalline volume fraction and large grain size can be produced by control1ing the behavior of the charged clusters generated in the gas phase of a normal HWCVD reactor.

• Journal of Korean Neurosurgical Society
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• 제30권4호
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• pp.417-424
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• 2001

Objective : Adrenal medullary chromaffin cells are known to release analgesic substances such as opioides and catecholamines. Transplantation of them is a novel method that challenges current approaches in treating chronic pain. The transplantation of xenogeneic chromaffin cells into the central nervous system(CNS) supply antinociception in animals. In this study, we investigated the analgesic effects of rat adrenal medullary chromaffin cells transplanted into the CNS of the mouse. To study the antinociceptive efficacy of transplanted chromaffin cells, the survival of rat adrenal medullary chromaffin cells transplanted into the CNS of mouse was determined. Methods : The adrenal medullary chromaffin cells isolated from rat were transplanted into the striatum of mouse. These cells were confirmed of the release of Met-enkephalin and Leu-enkephalin by HPLC, and immunoblots for tyrosine hydroxylase(TH). Two weeks after transplantation, we performed immunohistochemistry for TH to determine the survival of implanted cells and assessed pain sensitivity at the same time. Results : The isolated rat adrenal medullary chromaffin cells were positive for anti-TH antibody and released Met-enkephalin and Leu-enkephalin more than rat endothelial cells. Transplanted rat chromaffin cells were stained with anti-TH antibody in striatum of mouse after 2 weeks. Pain sensitivity was reduced on the chromaffin cell-transplanted mouse compared to endothelial cell-transplanted mouse by the hot plate test. Conclusion : These results suggest that the rat chromaffin cells were suitably transplanted into the CNS of mouse. This approach could be used as a therapy for reducing of chronic pain induced by cancer or neuronal injury.

• Current Research on Agriculture and Life Sciences
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• 제7권
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• pp.117-125
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• 1989

벼잎선충(線蟲) 방제(防除)를 위(爲)한 볍씨의 온탕침적조건(溫湯浸績條件), 벼품종간(品種間)의 저항성(抵抗性) 정도(程度), 피해경율(被害莖率)과 수량감소(收量減少) 정도(程度) 등을 구명(究明)하기 위(爲)해 시험(試驗)한 결과(結果)를 요약(要約)하면 다음과 같다. 볍씨 온탕침법(溫湯浸法)에 의(依)한 선충(線蟲) 방제효과(防除效果)는 $61^{\circ}C$에서 10-15분간(分間) 처리(處理)하였을때 가장 좋았다. 벼잎선충(線蟲)에 대한 품종간(品種間)의 피해경율(被害莖率)은 통일형(統一型) 품종(品種)의 경우 White tip 증상(症狀)이 나타나지 않았고 일본형(日本型) 품종(品種)들은 대부분 White tip 증상(症狀)이 잘 나타났는데 그 중(中)에서도 오태(五台)벼와 부광벼는 각각(各各) 95.6%, 81.8%의 높은 피해경율(被害脛率)을 보였다. 그러나 볍씨내 선충밀도(線蟲密度)에 있어서는 통일형(統一型), 일본형(日本型) 품종(品種) 모두 벼 10g당 100마리 이상(以上) 검출되었다. White tip 증상(症狀)이 나타나지 않았던 통일형(統一型) 품종(品種)들도 선충(線蟲) 접종구(接種區)에서는 수량(收量) 및 수량구성요소(收量構成要素)가 감소(減少)되는 품종(品種)이 많았고 일본형(日本型) 품종(品種)에서는 피해경율(被害莖率)이 높았던 오태(五台)벼와 부광벼는 40% 가까운 수량감소율(收量減少率)을 보였으며 전체적(全體的)으로 통일형(統一型) 보다는 일본형(日本型) 품종(品種)에서 수량구성요소(收量構成要素)와 수량(收量)의 감수정도(減收程度)가 높게 나타났다. 일본형(日本型) 품종(品種) 중(中) 영덕(盈德)5호(號)는 접종구(接種區)에서 White tip 증상(症狀)이 전혀 나타나지 않았고 수량(收量)도 감소(減少)되지 않아 벼잎선충(線蟲)에 대하여 저항성(抵抗性) 품종(品種)으로 생각된다.

• 한국콘텐츠학회논문지
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• 제11권4호
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• pp.273-282
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• 2011

본 연구는 국소 허혈성 뇌손상 흰쥐 모델에서 tDCS의 자극 적용시간을 달리하였을 때, 앞다리 운동감각 기능변화와 신경영양인자(GAP-43)발현에 미치는 영향을 알아보고자 하였다. 뇌손상 모델은 Sprague -Dawley계 흰쥐 80마리를 'Longa'방법을 이용하여 중대뇌동맥(middle cerebral artery)을 폐색하여 유발하였고, 실험군을 4개로 나누었다; 실험군I은 허혈성 뇌손상 유발군(n=20), 실험군II는 허혈성 뇌손상 유발 후 tDCS(10분) 적용군(n=20), 실험군III은 허혈성 뇌손상 유발 후 tDCS(20분) 적용군(n=20), 실험군IV는 허혈성 뇌손상 유발 후 tDCS(30분) 적용군(n=20)으로 나누었다. 앞다리운동감각 기능검사를 위해 수정된 앞다리배치 검사와 단일 팰릿 닿기 검사를 실시하였으며, 신경가소성에 대한 면역조직화학적 검사로 운동감각 영역에서의 GAP-43 단백질 발현을 관찰하였다. 앞다리운동감각 검사는 14일에서 실험군III (p<0.05)이 다른 군들에 비해 유의한 차이를 보였으며, 단일 팰릿 닿기 검사는 14일에서 실험군III(p<0.01)과 실험군IV(p<0.05)에서 유의한 차이를 보였다. 또한, 면역조직학적 검사는 14일에 실험군III이 다른 군들에 비해 현저한 면역양성반응의 증가를 보였다. 따라서, 0.1 mA의 강도로 20분간 적용했을 때가 앞다리운동감각 기능과 신경가역성 인자 GAP-43 발현에 가장 좋은 조건임을 알 수 있었다.

• Journal of Microbiology and Biotechnology
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• 제18권6호
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• pp.1179-1185
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• 2008

The immune-stimulating activities of Bordetella bronchiseptica antigens containing dermonecrotoxin (BBD) loaded in chitosan microspheres (CMs) have already been reported in vitro and in vivo with a mouse alveolar macrophage cell line (RAW264.7) and mice. Therefore, this study attempted to demonstrate the successful induction of mucosal immune responses after the intranasal administration of BBD loaded in CMs (BBD-CMs) in colostrum-deprived pigs. The BBD was introduced to the CMs using an ionic gelation process involving tripolyphosphate (TPP). Colostrum-deprived pigs were then directly immunized through intranasal administration of the BBD-CMs. A challenge with a field isolate of B. bronchiseptica was performed ten days following the final immunization. The BBD-specific IgG and IgA titers, evident in the nasal wash and serum from the vaccinated pigs, increased with time (p<0.05). Following the challenge, the clinical signs of infection were about 6-fold lower in the vaccinated pigs compared with the nonvaccinated pigs. The grades for gross morphological changes in the turbinate bones from the vaccinated pigs were also significantly lower than the grades recorded for the nonvaccinated pigs (p<0.001). Therefore, the mucosal and systemic immune responses induced in the current study would seem to indicate that the intranasal administration of BBD-CMs may be an effective vaccine against atrophic rhinitis in pigs.

• 한국터널지하공간학회 논문집
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• 제15권3호
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• pp.333-344
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• 2013

본 연구에서는 절리 암반 내 터널굴착 시 지하수 유출량 예측량이 실제 계측치와 큰 차이가 나는 이유 중 하나인 터널주변 절리암반의 투수계수의 감소 현상에 대해 논의하였다. 현재 터널 설계 시 일반적으로 사용되고 있는 지하수 유출량 산정식은 터널주변 암반이 등방, 균질하고 일정한 투수계수를 유지한다고 가정한다. 하지만, 실제로는 터널주변 절리암반의 투수계수는 터널주변 유효응력 상태에 따라 변화하며, 절리 내 지하수 흐름에 따라 다시 터널주변 유효응력 분포가 영향을 받는 수리-역학적 상호거동을 보인다. 터널굴착 직후 터널 접선방향 유효응력이 응력집중과 간극수압 감소로 인해 급증하고 그에 따라 절리의 닫힘현상이 발생하며, 결과적으로 터널인접 절리암반 링 구간에서 투수계수가 급격히 감소하게 된다. 이러한 터널인접 링 구간 내에서 상당히 큰 간극수압 감소가 발생하게 되어 터널주변 간극수압 분포는 등방 균질의 절리암반으로 가정한 산정식과 큰 차이를 보인다. 본 연구에서는 절리암반의 수리-역학적 상호거동의 개념을 도입하여 터널주변 간극수압 분포와 터널 내 지하수 유입량 산정방법을 제안하고 이를 수치해석을 통해 검증하였다.

• 한국해양환경ㆍ에너지학회지
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• 제19권3호
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• pp.194-202
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• 2016

본 연구에서는 10 MW급 부유식 파력-풍력 복합발전 시스템의 플랫폼 초기 개념설계를 위해 유한요소해석 기반 위상 최적화를 검토하였다. 실제 파력-풍력 복합발전 시스템 플랫폼의 위상최적화를 수행하기 전에 단순화된 구조설계 문제를 이용하여 효율적인 위상최적화 이론을 확인하고자 밀도법과 균질화설계법의 두 가지 위상최적화 이론을 적용하였다. 단순화된 설계 문제의 결과로부터 균질화설계법 이론을 파력-풍력 복합발전 시스템의 플랫폼 위상최적화에 적용하였다. 파력-풍력 복합발전 시스템의 플랫폼 개념설계를 위해서 유한요소해석 모델을 생성하고 설치해역의 해양환경하중을 고려하여 구조해석을 수행하였다. 설계파 및 조류와 같은 해양환경하중으로부터 기인하는 플랫폼 상의 압력과 계류삭의 인장력을 산출하기 위하여 동수력학 해석을 수행하였다. 구조해석을 위한 하중조건은 부유체 동수력학 해석으로부터의 결과와 파력-풍력 복합발전 시스템 중량을 고려하였고, 경계조건은 관성제거법을 사용하여 구현하였다. 밀도법 기반 파력-풍력 복합발전 시스템 플랫폼의 위상최적화 결과로부터 개념설계 단계에서 주요 구조부재의 배치방안을 제시하였다. 본 연구결과로부터 위상최적화는 부유식 파력-풍력 복합발전 시스템과 같은 새로운 형식의 해양구조물 개발에 있어서 주요 구조부재 배치의 개념설계에 대해 유용한 설계도구임을 확인하였다.

• Nutrition Research and Practice
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• 제9권2호
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• pp.129-136
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• 2015

BACKGROUND/OBJECTIVES: A variety of immunomodulators can improve the efficacy of low-dose chemotherapeutics. Active hexose correlated compound (AHCC), a mushroom mycelia extract, has been shown to be a strong immunomodulator. Whether AHCC could enhance the antitumor effect of low-dose 5-fluorouracil (5-FU) via regulation of host immunity is unknown. MATERIALS/METHODS: In the current study Hepatoma 22 (H22) tumor-bearing mice were treated with PBS, 5-FU ($10mg{\cdot}kg^{-1}{\cdot}d^{-1}$, i.p), or AHCC ($360mg{\cdot}kg^{-1}{\cdot}d^{-1}$, i.g) plus 5-FU, respectively, for 5 d. $CD^{3+}$, $CD^{4+}$, $CD^{8+}$, and NK in peripheral blood were detected by flow cytometry. ALT, AST, BUN, and Cr levels were measured by biochemical assay. IL-2 and $TNF{\alpha}$ in serum were measured using the RIA kit and apoptosis of tumor was detected by TUNEL staining. Bax, Bcl-2, and TS protein levels were measured by immunohistochemical staining and mRNA level was evaluated by RT-PCR. RESULTS: Diet consumption and body weight showed that AHCC had no apparent toxicity. AHCC could reverse liver injury and myelosuppression induced by 5-FU (P < 0.05). Compared to mice treated with 5-FU, mice treated with AHCC plus 5-FU had higher thymus index, percentages of $CD^{3+}$, $CD^{4+}$, and NK cells (P < 0.01), and ratio of $CD^{4+}$/$CD^{8+}$ (P < 0.01) in peripheral blood. Radioimmunoassay showed that mice treated with AHCC plus 5-FU had the highest serum levels of IL-2 and $TNF{\alpha}$ compared with the vehicle group and 5-FU group. More importantly, the combination of AHCC and 5-FU produced a more potent antitumor effect (P < 0.05) and caused more severe apoptosis in tumor tissue (P < 0.05) compared with the 5-FU group. In addition, the combination of AHCC and 5-FU further up-regulated the expression of Bcl-2 associated X protein (Bax) (P < 0.01), while it down-regulated the expression of B cell lymphoma 2 (Bcl-2) (P < 0.01). CONCLUSIONS: These results support the claim that AHCC might be beneficial for cancer patients receiving chemotherapy.

• 한국터널지하공간학회 논문집
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• 제22권1호
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• pp.23-46
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• 2020

본 연구에서는 터널 근접 시공으로 인한 기 존재 단독말뚝의 공학적 거동을 파악하기 위하여 터널로부터 말뚝선단의 이격거리와 막장압의 변화를 고려한 3차원 유한요소해석을 수행하였다. 수치해석에서는 터널 막장압을 고려하여 말뚝의 거동을 분석하였으며, 터널굴착으로 유발되는 지반침하, 말뚝두부침하, 말뚝축력 및 말뚝-지반 사이의 경계면에서 발생하는 전단응력을 고찰하였다. 말뚝이 터널 크라운(crown) 바로 상부에 위치하고 말뚝선단까지의 수직 이격거리가 0.25D (여기서, D는 터널직경)인 경우 초기 응력의 50%에 해당하는 막장압을 적용할 경우 25%의 막장압을 적용한 것과 비교한 결과 말뚝두부의 침하가 약 38% 감소하였다. 또한, 막장압의 크기가 작을수록 지반침하, 말뚝의 축력 및 말뚝-지반 사이에서 발생하는 전단응력이 증가하며, 말뚝이 터널굴착 영향권 밖에 존재할 경우 말뚝에는 압축력 형태의 축력이 발생하였다. 따라서 막장압의 크기 및 터널-말뚝선단의 상대위치는 지반 침하와 말뚝 침하에 큰 영향을 미치는 것으로 분석되었다. 본 연구에서 수행된 연구결과의 경우 기존에 보고된 연구결과를 바탕으로 비교분석을 실시하였으며, 터널굴착으로 인한 말뚝의 거동을 심도 있게 분석하였다.

• 미술이론과 현장
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• 제2호
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• pp.49-74
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• 2004

It has been frequently pointed out that the established art history with the stylistic and iconographic interpretations and monographic analysis is fallen behind the currency of modern art. Among those who claimed the crisis in the discipline of art history, there is a suggestion that the art historical study should be fostered by other factors in the fields of the humanities. The so called New Art History or 'visual Culture Studies' insists that art history has to be restructured to integrate the broader study of culture and society, and by now, such an opinion is not a novelty at all. One of the most significant yet overlooked elements that induced the new currency of art history is properties of contemporary art that conflict the traditional claim of art historians. Although the idea that art is not purely aesthetic but that it has many other functions has been brought up by the art historians, it was the artists that provoked such a perception. When Arthur C. Danto and Hans Belting proclaimed the End of Art and Art History in the 1980s, the concept of art has been changed radically through the avant-garde tendency of Modernism and a new pluralism of Postmodernism. One dominant concern that strikes art historians is to find a new approach to art, since the traditional method and goal of analysis for past art and past art history seem unavailable. The perplexity arising from the situation is intensified in the field of teaching art, especially for those who teach art history in art school. Basically art history is a pursuit of learning of art in history, and its purpose is to reconcile the present with the past and the future as well. Since Modernism, as it is confusing sometimes because it implies the present state, somehow art became considered 'tradition-less'. It does not mean that a work of art stands aloof from the past attainments, hut modern art imposed itself on a task seeking after the new for its own sake, turning its back on the tradition. And now in the era of Postmodernism, an historians face the requirement to revaluate the whole history of art including modernism. The necessity of art history in art education is indisputable, but methods and contents in the academic courses should he reexamined now. Because artists' concept of history and past art has been altered, and art history as a humanistic discipline can only maintain its identity through incorporation with art itself. Academics teaching art history, or, strictly speaking, past works of art and history, to the student in art school, confront with the need to rethink the object of art history and its meaning to the artists.