• Mycobiology
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• 제46권2호
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• pp.85-91
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• 2018

Endophytic fungi strains (n = 81) were isolated from the leaves, barks, and fruits of Camellia oleifera from Hunan province (China) to delineate their species composition and potential as biological control agents of C. oleifera anthracnose. The fungi were identified by morphological and phylogenetic analyses. Fungal colonization rates of the leaves, barks, and fruits were 58.02, 27.16, and 14.81%, respectively. The isolates were identified as 14 genera, belonging to two subdivisions, Deuteromycotina and Ascomycotina; 87.65% of all isolates belonged to Deuteromycotina. The dominant species, occurring with a high relative frequency, were Pestalotiopsis sp. (14.81%), Penicillium sp. (14.81%), and Fusarium sp. (12.35%). The Simpson's and Shannon's diversity indices revealed the highest species diversity in the leaves, followed by the barks and fruits. The similarity index for the leaves versus barks comparison was the highest, indicating that the number of endophytic fungal species shared by the leaves and barks was higher than barks and fruits or leaves and fruits. Based on the results of dual culture experiments, only five strains exhibited antifungal activity against C. oleifera anthracnose pathogen, with isolate ty-64 (Oidium sp.) generating the broadest inhibition zones. Our results indicate that the endophytes associated with C. oleifera could be employed as natural agents controlling C. oleifera anthracnose.

• 환경생물
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• 제38권4호
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• pp.594-602
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• 2020

지형과 고도가 논둑에 서식하는 토양성거미의 군집과 다양성에 미치는 영향을 조사하였다. 지형의 영향평가를 위해 벼재배기에 산간지와 평야지에서 비교한 결과 거미의 군집구조가 달랐으며 발생밀도는 산간지보다 평야지에서 높았다. 늑대거미과가 가장 우점하였으며 이사고늑대거미(P. isago)의 발생밀도는 산간지보다 평야지에서 높았다. 거미군집의 발생은 국지(100 km²) 및 지역(1,000 km²) 범위에서 유사하였다. 고도의 영향과 관련하여 휴한기에 지형이 유사한 장수와 정읍 지역을 비교한 결과 발생밀도는 지역 간 차이가 없었으나, 긴마디늑대거미속(Pardosa spp.) 3종의 발생밀도는 정읍에서 높았다. 이러한 결과는 지형과 고도가 군집 수준에서 토양성 논거미의 다양성지수에 미치는 영향은 크지 않으나, 종 수준에서 논에 의존적인 늑대거미과의 우점종에 영향을 줄 수 있다는 것을 보여준다.

• The Korean Journal of Physiology and Pharmacology
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• 제17권6호
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• pp.517-523
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• 2013

Naphthyridine compounds are important, because they exhibit various biological activities including anticancer, antimicrobial, and anti-inflammatory activity. Some naphthyridines have antimitotic effects or demonstrate anticancer activity by inhibiting topoisomerase II. These compounds have been investigated as potential anticancer agents, and several compounds are now part of clinical trials. A series of naphthyridine derivatives were evaluated for their in vitro cytotoxic activities against human cervical cancer (HeLa), leukemia (HL-60), and prostate cancer (PC-3) cell lines using an MTT assay. Some compounds (14, 15, and 16) were more potent than colchicine against all three human cancer cell lines and compound (16) demonstrated potency with $IC_{50}$ values of 0.7, 0.1, and $5.1{\mu}M$, respectively. Comparative molecular field analysis (CoMFA) and comparative molecular similarity indices analysis (CoMSIA) were used for quantitative structure-activity relationship (QSAR) molecular modeling of these compounds. We obtained accurate and predictive three-dimensional QSAR (3D-QSAR) models as indicated by the high PLS parameters of the HeLa ($q^2$, 0.857; $r^2$, 0.984; $r^2\;_{pred}$, 0.966), HL-60 ($q^2$, 0.777; $q^2$, 0.937; $r^2\;_{pred}$, 0.913), and PC-3 ($q^2$, 0.702; $q^2$, 0.983; $r^2\;_{pred}$, 0.974) cell lines. The 3D-QSAR contour maps suggested that the C-1 NH and C-4 carbonyl group of the naphthyridine ring and the C-2 naphthyl ring were important for cytotoxicity in all three human cancer cell lines.

• Bulletin of the Korean Chemical Society
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• 제32권5호
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• pp.1604-1612
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• 2011

We developed three-dimensional quantitative structure activity relationship (3D-QASR) models for 17 adamantyl derivatives as P-glycoprotein (P-gp) inhibitors. Eighteen different partial charge calculation methods were tested to check the feasibility of the 3D-QSAR models. Best predictive comparative molecular field analysis (CoMFA) model was obtained with the Austin Model 1-Bond Charge Correction (AM1-BCC) atomic charge. The 3D-QSAR models were derived with CoMFA and comparative molecular similarity indices analysis (CoMSIA). The final CoMFA model ($q^2$ = 0.764, $r^2$ = 0.988) was calculated with an AM1-BCC charge and electrostatic parameter, whereas the CoMSIA model ($q^2$ = 0.655, $r^2$ = 0.964) was derived with an AM1-BCC charge and combined steric, electrostatic, hydrophobic and HB-acceptor parameters. Leave-five-out (LFO) cross-validation was also performed, which yielded good correlation coefficient for both CoMFA (0.801) and CoMSIA (0.656) models. Robustness of the developed models was checked further with 1000 run bootstrapping analyses, which gave an acceptable correlation coefficient for CoMFA (BS-$r^2$ = 0.997, BS-SD = 0.003) and CoMSIA (BS-$r^2$ = 0.996, BS-SD = 0.018).

• 한국산림과학회지
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• 제97권2호
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• pp.171-180
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• 2008

The characterization of tree species and forest stand conditions is useful in the planning of activities aimed to conserve biodiversity. The main objective of this study was to describe tree species diversity, species composition and stand structure of tropical deciduous forests distributed in three regions in Myanmar. Forest inventory was conducted in the Oktwin teak bearing forest, the Letpanpin community forest and Alaungdaw Kathapa National Park. According to the Jackknife estimator of species richness, 85 species (${\pm}18.16$), 70 species (${\pm}5.88$) and 186 species (${\pm}17.10$) belonging to 31 families were found in the Oktwin teak bearing forest, 33 families in Letpanpin community forest and 53 families in Alaungdaw Kathapa national park, respectively. Shannon's diversity indices were significantly different among the forests (p<0.05). It ranged from 3.36 to 4.36. Mean tree density (n/ha) of the Oktwin teak bearing forest, Letpanpin community forest and Alaungdaw Kathapa National Park were 488 (${\pm}18.6$), 535 (${\pm}15.6$) and 412 (${\pm}14.1$), while basal areas per hectare were $46.96m^2({\pm}3.23),\;49.01m^2({\pm}5.08)\;and\;60.03m^2({\pm}3.88)$, respectively. At the family level, Verbenaceae, Myrtaceae and Combretaceae occupied the highest importance value index, while at the species level it was Tectona grandis, Lagerstoremia speciosa and Xylia xylocarpa.

• Bulletin of the Korean Chemical Society
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• 제29권3호
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• pp.647-655
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• 2008

Microsomal prostaglandin E2 synthase (mPGES-1) is a potent target for pain and inflammation. Various QSAR (quantitative structure activity relationship) analyses used to understand the factors affecting inhibitory potency for a series of MK886 analogues. We derived four QSAR models utilizing various quantum mechanical (QM) descriptors. These QM models indicate that steric, electrostatic and hydrophobic interaction can be important factors. Common pharmacophore hypotheses (CPHs) also have studied. The QSAR model derived by best-fitted CPHs considering hydrophobic, negative group and ring effect gave a reasonable result (q2 = 0.77, r2 = 0.97 and Rtestset = 0.90). The pharmacophore-derived molecular alignment subsequently used for 3D-QSAR. The CoMFA (Comparative Molecular Field Analysis) and CoMSIA (Comparative Molecular Similarity Indices Analysis) techniques employed on same series of mPGES-1 inhibitors which gives a statistically reasonable result (CoMFA; q2 = 0.90, r2 = 0.99. CoMSIA; q2 = 0.93, r2 = 1.00). All modeling results (QM-based QSAR, pharmacophore modeling and 3D-QSAR) imply steric, electrostatic and hydrophobic contribution to the inhibitory activity. CoMFA and CoMSIA models suggest the introduction of bulky group around ring B may enhance the inhibitory activity.

• Journal of Contemporary Eastern Asia
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• 제19권1호
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• pp.43-58
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• 2020

This study examines media used for information in the East Asian countries of China, Japan, South Korea, Taiwan, and Singapore, using data from the World Values Survey. The sharing of Confucian culture may lead to a uniform media structure across these nations. Another possibility is technological determinism, which would also lead to similarity across nations. However, it is possible that countries are at different stages of technology development and will eventually become more similar. An opposing notion is that differences in other values among nations predict digital media use. To examine the evidence considering these possibilities, we factor analyze each population's use of nine traditional and digital media to see how similar the structures are. What results is a three-dimensional solution for four out of five countries, except Singapore, which has a more simple two-dimensional structure. Analysts regard Singapore as the most digitally connected society, which raises the question as to whether it is higher on a technological development trajectory, to which other countries may transition. Perhaps a more simple media use structure is an adaptation to increasing information load. As well, as mobile devices have become a primary means of accessing the range of traditional and social media, it may have an expanded role in reducing media channel entropy. In terms of frequency of media use, Singapore is highest, while China is the lowest. Singapore stands out in high mobile use, and China for low Internet use. There appear to be developmental differences across the nations. Regressions on Internet use for 18 values indices find different values predictors in the East Asian countries, ruling out Confucianism as producing similar media patterns.

• 정보처리학회논문지B
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• 제16B권1호
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• pp.71-78
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• 2009

보다 효과적인 색인어 추출 및 색인어 가중치 결정을 위하여 문서의 내용뿐 아니라 구조를 이용하여 색인을 추출하는 연구가 이루어지고 있는데, 대부분의 연구들이 XML 태그의 중요도가 아닌, 문맥상의 단락에 대한 중요도를 계산하는게 일반적이다. 이러한 기존 연구들은 대부분이 객관적인 실험을 통해서 중요도를 입증하기보다는 상식적인 관점에서 단순한 수치로 중요도를 결정하고 있다. 본 논문에서는 웹 문서 관리를 위한 표준으로 자리잡아가고 있는 XML 문서의 태그 정보를 이용한 자동색인을 위하여, 논문을 구성하는 주요 태그를 중요도에 따라 분류하고, 낮은 태그에서 추출된 용어 가중치를 계산하고, 그 가중치로 높은 가중치의 태그에서 추출된 용어의 가중치를 갱신해 가면서 최종 가중치를 계산하는 방법을 제안한다. 보다 객관적인 가중치 결정을 위하여 사용자가 중요하게 생각하는 태그를 실험해 보고 그에 따라 중요도를 분류하여 가중치 계산에 반영한다. 그리고 기존 태그 중요도 결정 방법을 적용하여 계산된 색인어 가중치를 이용한 검색성능과 비교함으로써 본 논문에서 제안한 방법을 적용하여 계산된 색인어 가중치의 효과를 검증한다.

• 한국지능시스템학회논문지
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• 제6권3호
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• pp.81-88
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• 1996

클러스터링은 주어진 데이타 집합의 패턴을 비슷한 성질을 가지는 그룹으로 나누어 패턴 상호간의 관계를 정립하기 위한 방법론이다. 이러한 클러스터링 기법을 위하여 많은 알고리즘이 개발되었고, 패턴인식과 영상처리 등의 여러 공학영역에 적용되어 왔다. 대부분의 실세계 데이타는 그 경계가 명확하지 않으므로 그 특성을 보다 정확히 반영하기 위하여 퍼지이론이 도입되었다.이와 같은 클러스터 분석 방법은 보다 적절히 으용하기 위하여 클러스터링의 적절성을 평가하기 위한 방법론과 함께 연구되어야 한다. 이를 위하여 각 데이타 패턴이 얼마나 잘 분류되었는지를 수학적으로 계산하기 위한 함수들이 제안되었다. 그러나 클로스터 타당성 문제는 주어지 클러스터링 방법론의 특성, 그 알고리즘에서 사용한 파라메터의 성질, 주어진 입력 데이타 집합의 특성 등 여러 복잡한 상황을 포함하고 있으므로 기존의 연구에서와 같이 하나의 함수를 이용하여 해결하기는 어렵다. 그러므로 본 논문에서는 기존에 연구되어온 타당성 측정 함수를 조사하고 그의 단점을 고찰하여 이를 해결하기 위한 방법으로 4가지성능 측정자를 제안하고 이의 결합에 의하여 형성된 클러스터 타당성의 정도를 구하는 방법론을 제시하고자 한다. 또한 이러한 방법은 퍼지 클러스터링을 위한 학습 알고리즘과 결함하여 클러스터의 수나 데이타의 분포에 대한 정보없이 최적 클러스터를 찾아주는 방법에 응용될 수 있음을 보인다.

• Applied Biological Chemistry
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• 제48권3호
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• pp.252-257
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• 2005

새로운 2-(4-(6-chloro-2-benzoxazolyloxy)phenoxy)-N-phenylpropionamide 유도체들의 구조 변화에 따른 발아 전, 논피(Echinochloa crus-galli)에 대한 제초활성과의 3D-QSAR 관계를 상이한 정렬방법에 따라 비교 분자장 분석(CoMFA)과 비교분자 유사성 지수분석(CoMSIA) 방법으로 연구하였다. 가장 양호한 3D-QSAR 모델은 atom based fit 정렬과 CoMFA장과 CoMSIA장의 조합 조건에서 유도된 CoMFA 모델(AI-2)과 CoMSIA 모델(AII-4)이었다. CoMFA 및 CoMSIA 등고도로부터 제초활성은 N-phenyl 고리 상 치환기의 구조변화로 개선될 수 있었다.