• Journal of Microbiology and Biotechnology
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• v.20 no.8
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• pp.1163-1178
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• 2010

Plasmids play important roles in horizontal gene transfer among Vibrionaceae, but surprisingly little is known about their replication and incompatibility systems. In this study, we successfully developed a bioinformatics-assisted strategy of experimental identification of seven Vibrio plasmid replicons. Comparative sequences analysis of the seven Vibrio plasmid replicons obtained in this study together with eight published Vibrionaceae plasmid sequences revealed replication-participating elements involved in the ColE1 mode of replication initiation and regulation. Like plasmid ColE1, these Vibrionaceae plasmids encode two RNA species (the primer RNA and the antisense RNA) for replication initiation and regulation, and as a result, the 15 Vibrionaceae plasmids were designated as ColE1-like Vibrionaceae (CLV) plasmids. Two subgroups were obtained for the 15 CLV plasmids, based on comparison of replicon organization and phylogenetic analysis of replication regions. Coexistence of CLV plasmids were demonstrated by direct sequencing analysis and Southern hybridization, strongly suggesting that the incompatibility of CLV plasmids is determined mainly by the RNA I species like the ColE1-like plasmids. Sequences resembling the conserved Xer recombination sites were also identified on the CLV plasmids, indicating that the CLV plasmids probably use the host site-specific recombination system for multimer resolution like that used by ColE1-like plasmids. All the results indicated that the 15 plasmids form a new ColE1-like group, providing a basis for the rapid characterization and classification of Vibrionaceae plasmids.

• Korea Journal of Hospital Management
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• v.23 no.2
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• pp.95-105
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• 2018

Objective: This study conducted an empirical research on foreign patients who had visited the medical institutions of Daegu region in order to identify the effects of the factor satisfaction and experience satisfaction with medical services on their revisit intentions. Methods: This study reviewed the existing studies on medical services to foreign patients with a main focus on medical tourism, and conducted the empirical research through a survey on foreign patients from the Russian, Chinese and English-speaking regions based on the Daegu Medical Tourism Promotion Institute's DB of foreign patients. The empirical research conducted a multiple regression analysis on factor satisfaction and experience satisfaction regarding return visit intentions. Results: The multiple regression analysis results showed that both the factor satisfaction and the experience satisfaction with medical services of foreign patients had positive effects on their return visit intentions, especially, with a relatively greater effect from the factor satisfaction. Specifically, it was found that factor satisfaction elements of 'friendliness of employees', 'good communication', and 'expertise of medical workers'; and experience satisfaction elements of 'reliability of medical information', 'efforts to minimize incompatibility towards foreigners', 'convenient access to medical information' and the like enhanced the return visit intentions. Conclusion: We discovered that, in the context of medical tourism activities, it is important to enhance factor satisfaction and experience satisfaction with medical services in order to promote return visits of the foreign patients who we once attracted. Especially, there are implications that reliability of medical information and efforts to minimize incompatibility towards foreigners enhance return visits of foreign patients.

• Steel and Composite Structures
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• v.7 no.6
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• pp.421-440
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• 2007

An optimal design method in cooperated with nonlinear inelastic analysis is presented. The proposed nonlinear inelastic method overcomes the difficulties due to incompatibility between the elastic global analysis and the limit state member design in the conventional LRFD method. The genetic algorithm used is a procedure based on Darwinian notions of survival of the fittest, where selection, crossover, and mutation operators are used to look for high performance ones among sections in the database. They are satisfied with the constraint functions and give the lightest weight to the structure. The objective function taken is the total weight of the steel structure and the constraint functions are load-carrying capacity, serviceability, and ductility requirement. Case studies of a planar portal frame, a space two-story frame, and a three-dimensional steel arch bridge are presented.

• Special Issue of the Society of Naval Architects of Korea
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• 2005.06a
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• pp.134-137
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• 2005

Recently, in VLCC, shafting system is stiffer due to large engine power whereas hull structure is more flexible due to scantling optimization, which can be suffered from alignment damage by incompatibility between shafting and hull, In this study, shafting system without stern tube forward bush was adapted for less sensitive system against external factors. Also, shaft alignment analysis was considered with hull deflection at various ship loading conditions and stern tube after bush of long journal bearing was evaluated by static squeezing pressure and dynamic oil film pressure with sloping control. Whirling vibration was also reviewed to avoid resonance with propeller blade order. So, reliable shafting design for VLCC could be achieved through optimized alignment analysis for the system without stern tube forward bush.

• Proceedings of the Computational Structural Engineering Institute Conference
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• 2003.10a
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• pp.145-152
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• 2003

An optimal design method in cooperated with nonlinear inelastic analysis method is presented. The proposed nonlinear inelastic method overcomes the difficulties due to incompatibility between the elastic global analysis and the limit state member design in the conventional LRFD method. The genetic algorithm uses a procedure based on Darwinian notions of survival of the fittest, where selection, crossover, and mutation operators are used among sections in the database to look for high performance ones. They satisfy the constraint functions and give the lightest weight to the structure. The objective function is set to the total weight of the steel structure and the constraint functions are load-carrying capacities, serviceability, and ductility requirement. Case studies of a three-dimensional frame and a three-dimensional steel arch bridge are presented.

• Applied Microscopy
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• v.50
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• pp.12.1-12.11
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• 2020

Hot stage microscopy (HSM) is a thermal analysis technique that combines the best properties of thermal analysis and microscopy. HSM is rapidly gaining interest in pharmaceuticals as well as in other fields as a regular characterization technique. In pharmaceuticals HSM is used to support differential scanning calorimetry (DSC) and thermo-gravimetric analysis (TGA) observations and to detect small changes in the sample that may be missed by DSC and TGA during a thermal experiment. Study of various physical and chemical properties such sample morphology, crystalline nature, polymorphism, desolvation, miscibility, melting, solid state transitions and incompatibility between various pharmaceutical compounds can be carried out using HSM. HSM is also widely used to screen cocrystals, excipients and polymers for solid dispersions. With the advancements in research methodologies, it is now possible to use HSM in conjunction with other characterization techniques such as Fourier transform infrared spectroscopy (FTIR), DSC, Raman spectroscopy, scanning electron microscopy (SEM) which may have additional benefits over traditional characterization techniques for rapid and comprehensive solid state characterization.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.51 no.spc1
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• pp.264-268
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• 2006

This study investigated the effect of self-incompatibility control substance 'Apple plus' in improving self-fertilization and quality on apple. Results showed that the rate of fruit setting of disc florets of Fuji apple in the open field increased from 17.2% in 2004 to 44.4% in 2005 for the treated plots and from 0.5% in 2004 to 3.0% in 2005 for the control. In the case of using net covering, the rate of fruit setting increased from 3.3% in 2004 to 5.2% in 2005 for the treated plots and from 0.4% in 2004 to 0.1% in 2005 for the control. For the quality of apple fruits, results showed that there was no significant difference on the fruit L/D and firmness for both control and treated plots. However, the number of seeds was affected by the different substances which produced the following average number of seeds: control, 0.9; IS-1, 1.2; IS-2, 1.2; IS-3, 1.5 and IS-4, 0.7. The SSR genetic analysis showed that the rate of self-fertilization was highest for IS-1 (100%), followed by IS-2 (73%), IS-3 (68%), IS-4 (62%) and control (0%).

• Journal of Plant Biotechnology
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• v.42 no.3
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• pp.271-276
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• 2015

Crab apple cultivars, 'Maypole', 'Tuscan', and 'Manchurian', were evaluated as potential pollinizers of major apple cultivars, 'Fuji', 'Hongro', and 'Tsugaru', cultivated in high-density apple orchard systems. Numerous cultivar characteristics, including blooming time, pollen germination, fruit set, disease and pest resistance, and self-incompatibility, were examined. The blooming times of both 'Maypole' and 'Tuscan' ranged from April 19 to May 5, which was 2-4 days earlier than those of the major commercial apple cultivars. PCR analysis did not reveal the presence of any of the S-alleles (S1, S3, S7, or S9) identified in major commercial apple cultivars. In addition, the percentage of the fruit set was high after trees were artificially fertilized with crab apple pollen. Artificially cross-pollinated fruits were of similar or higher quality than open-pollinated fruits. They also demonstrated resistance to apple blotch, sooty blotch, and fly speck. The results indicate that the two crab apples, 'Maypole' and 'Tuscan' would be potential candidates for pollinizers of major apple cultivars in Korea. Use of the pollen of these crap apples in commercial production will improve fruit quality and promote sustainable and robust fruit production.

• Korean Journal of Plant Resources
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• v.24 no.6
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• pp.702-711
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• 2011

Buckwheat (Fagopyrum esculentum Moench), one of the minor crops grown in Korea belonging to the Polygonaceae family, is an annual crop widely cultivated in Asia, Europe, and America and has a character of outcrossing and self-incompatibility. The objective of this study was to analyze the genetic variability, phylogenetic relationships and population structure of buckwheat landraces of Korea using SSR markers. Ten microsatellite markers have been detected from a total of 79 alleles among the 179 buckwheat accessions were collected from Korea. The number of allele per marker locus ($N_A$) ranged from 2 (GB-FE-001, GB-FE-043 and GB-FE-055) to 31 (GB-FE-035) with an average of 7.9 alleles. GB-FE-035 was the most polymorphic with the highest PIC value 0.93. Major allele frequencies ($M_{AF}$) for the 10 polymorphic loci varied from 0.12 to 0.97 with a mean allele frequency of 0.57. The expected heterozygosity ($H_E$) values ranged from 0.05 to 0.94 with an average of 0.53. The observed heterozygosity ($H_O$) ranged from 0.06 to 0.92 with an average of 0.42. The overall polymorphic information contents (PIC) values ranged from 0.05 to 0.93 with an average of 0.48. The landrace accessions of buckwheat used in the present study were not distinctly grouped according to geographic distribution. The study concludes that the results revealed genetic differentiation was low according to the geographic region because of outcrossing and self-incompatibility. We reported that our analyses on the genetic diversity of common buckwheat cultivars of Korea were performed by using of microsatellite markers.

• Journal of Conservation Science
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• v.37 no.3
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• pp.255-269
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• 2021

A vast amount of materials-such as prints, woodblock prints, manuscripts, old novels, and letters-written in old Korean and using old grammar and/or obsolete characters, are collected in many institutions, including the Jangseogak at the Academy of Korean Studies. Digitization of these texts has required a prolonged manual inputting process. Individual researchers, who majored in old Korean, have read and typed the characters into electronic documents, which depends upon individual skill, effort, and approach, and is particularly limiting because none can be significantly increased. To date, only a small proportion of the old Korean document collections, currently kept in storage, have been digitized and made available to the public. Even the electronic formats of the texts prove difficult to displaying correctly, due to the incompatibility between the old Korean characters and the character set on today's electronic devices. To improve the techniques and efficiency of digitizing old Korean texts, it is necessary to develop optical character recognition (OCR), which will analyze images of old Korean documents, as well as input, display, and storage methods.