• Computers and Concrete
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• v.14 no.4
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• pp.463-477
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• 2014

The modelling approach in the case of connections in precast buildings is specific. The assembly of the constitutive parts of the connection requires the inclusion of contact definitions in the model. In addition, the material non-linearity including the influence of the spatial stress distribution should be taken into account where appropriate. Here a complex model of a beam-to-column dowel connection is presented. Experiments on the analysed connection were performed within the framework of the European project SAFECAST (Performance of Innovative Mechanical Connections in Precast Building Structures under Seismic Conditions). Several material and interaction parameters were investigated and the influence of each of them was evaluated. The set of parameters which gave the best match with the experiments was chosen.

• Proceedings of the KSRS Conference
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• 1999.11a
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• pp.271-280
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• 1999

The topographical effect resulted from rugged terrains and inhomogeneous spectral characteristics due to the complexly mixed land cover condition of Korea substantially lower the remotely sensed land cover classification accuracy In this study, a topographic correction method using digital elevation model to alleviate the topographic effects. To deal with inhomogeneous spectral characteristic, a hybrid classifier with inclusion of prior probabilities was introduced. This investigation concluded that the topographical normalization and hybrid classification with prior probabilities are effective on rugged landscape. The overall and average classification accuracies were improved by 0.92％ and 1.016％ respectively. The most substantial and noticeable accuracy improvement was observed in forest areas.

• 한국연소학회:학술대회논문집
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• 2001.11a
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• pp.125-135
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• 2001

Aiming at a direct, also more realistic, prediction of unstable waves evolving in the combustion chamber, present paper introduces a new analytical method. Instability equations are freshly formulated, and solve the time-integrated ODEs for amplification factors to find the transients of pressure and velocity fluctuations. Present numerical approach requires no separate treatments for nonlinearities. Preliminary numerical experiments for unstable waves in quasi-one-dimensional rocket combustor, show validity and applicability of present model, and promise for its practical use. Study for the complex models for physics, especially velocity- and pressure-coupled responses, and inclusion of multi dimensionality remains as future tasks.

• Communications of the Korean Mathematical Society
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• v.29 no.4
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• pp.527-538
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• 2014

Let K be a compact Hausdorff space, $\mathfrak{A}$ a commutative complex Banach algebra with identity and $\mathfrak{C}(\mathfrak{A})$ the set of characters of $\mathfrak{A}$. In this note, we study the class of functions $f:K{\rightarrow}\mathfrak{A}$ such that ${\Omega}_{\mathfrak{A}}{\circ}f$ is continuous, where ${\Omega}_{\mathfrak{A}}$ denotes the Gelfand representation of $\mathfrak{A}$. The inclusion relations between this class, the class of continuous functions, the class of bounded functions and the class of weakly continuous functions relative to the weak topology ${\sigma}(\mathfrak{A},\mathfrak{C}(\mathfrak{A}))$, are discussed. We also provide some results on its completeness under the norm defined by ${\mid}{\parallel}f{\parallel}{\mid}={\parallel}{\Omega}_{\mathfrak{A}}{\circ}f{\parallel}_{\infty}$.

• Journal of Microbiology and Biotechnology
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• v.25 no.2
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• pp.247-254
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• 2015

In this presentation, I describe the expression and purification of the recombinant liver X receptor β-ligand binding domain proteins in E. coli using a commercially available double cistronic vector, pACYCDuet-1, to express the receptor heterodimer in a single cell as the soluble form. I describe here the expression and characterization of a biologically active heterodimer composed of the liver X receptor β-ligand binding domain and retinoid X receptor α-ligand binding domain. Although many of these proteins were previously seen to be produced in E. coli as insoluble aggregates or "inclusion bodies", I show here that as a form of heterodimer they can be made in soluble forms that are biologically active. This suggests that co-expression of the liver X receptor β-ligand binding domain with its binding partner improves the solubility of the complex and probably assists in their correct folding, thereby functioning as a type of molecular chaperone.

• Macromolecular Research
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• v.14 no.2
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• pp.214-219
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• 2006

Cast Nafion-based composite membranes containing different amounts of organic, nanorod-shaped polyrotaxane were prepared and characterized, with the aim of improving the properties of polymer electrolyte membranes for direct methanol fuel cell applications. Polyrotaxane was prepared using the inclusion-complex reaction between ${\alpha}$-cyclodextrin and poly(ethylene glycol) (PEG) of different molecular weights. The addition of polyrotaxane to Nafion changed the morphology and reduced the crystallinity. The conductivity of the composite membranes increased with increasing polyrotaxane content up to 5 wt%, but then decreased at higher polyrotaxane contents. Well-dispersed, organic polyrotaxane inside the membrane can provide a tortuous path for the transport of methanol, as the methanol permeability depends on the aspect ratio of polyrotaxane, which is controlled by the molecular weight of PEG. All of the Nafion-based, polyrotaxane composite membranes showed a higher selectivity parameter than the commercial Nafion films did.

• Proceedings of the Polymer Society of Korea Conference
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• 2006.10a
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• pp.351-351
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• 2006

The possible interactions between cyclodextrins and biodegradable polyesters were investigated. The hydrophobicity of cyclodextrin could be varied with the methyl substitution of host CD, and the possibility of IC formation and the types of interaction between respective CDs and polyesters were subsequently changed. Further, the effect of cyclodextrins on the morphological change of biodegradable polymer was shown to depend on the degree of IC formation between cyclodextrin and biodegradable polymer as well as on the type of interaction between respective CDs and polyesters. That is, the enhancement and/or the restriction of the crystallization of P(3HB) were observed by the incorporation of various kind of cyclodextrins with different cavity size and hydrophobicity.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.65 no.1
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• pp.81-87
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• 2016

An LCL-type Isolated dc-dc converter operating for constant output voltage is analyzed, including the effect of a high frequency transformer using ac complex circuit approximation. Its solution is derived and is used to obtain the characteristics of the proposed converter. The analyses show through converter modeling, phasor diagram and gain comparison that inclusion of a high frequency transformer results in introduction of magnetizing inductance and leakage inductances at conventional LCL dc-dc converter with ideal transformer. The theoretical and simulation results are presented in case of the wide variations in input voltage and load current in detail. Analysis and simulation results observed that introduction of a transformer in the dc-dc converter had considerable effect on the performance, especially in the case of low output voltage and large load.

• Bulletin of the Korean Chemical Society
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• v.31 no.9
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• pp.2607-2612
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• 2010

Human tissue-type plasminogen activator (tPA) is a valuable thrombolytic agent used to successfully treat acute myocardial infarction, thromboembolic stroke, peripheral arterial occlusion, and venous thromboembolism. Recombinant tPA is accumulated as an inactive form in inclusion bodies of E. coli and is refolded in vitro, which is accompanied by extensive aggregation. In the present study, a tPA protease domain was expressed in an active soluble form in the cytosol of E. coli Rosetta-gami cells, which allowed disulfide bond formation and supplied the tRNA molecules required for six rarely used codons in E. coli. This strategy increased the amount of soluble protease domain protein and avoided the cumbersome refolding process. The purified protease domain not only degraded tPA substrate peptides but also formed a covalently bound complex with plasminogen activator inhibitor-1, as does full-length tPA. Soluble expression and purification of tPA domains may aid in functional analyses of this multi-domain protein, which has been implicated in many physiological and pathological processes.

• Bulletin of the Korean Chemical Society
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• v.35 no.7
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• pp.2114-2122
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• 2014

The association of silybin with ${\beta}$-cyclodextrin and its influence on silybin's binding with bovine serum albumin are reported. The stoichiometry, binding constant, and the structure of silybin-${\beta}$-cyclodextrin inclusion complex are reported. The titrations of silybin with bovine serum albumin in the absence and presence of ${\beta}$-cyclodextrin are carried out and the differences in binding strengths are discussed. Molecular modeling is used to optimize the sites and mode of binding of silybin with bovine serum albumin. F$\ddot{o}$rster resonance energy transfer is calculated and the proximity of interacting molecules is reported in the presence and absence of ${\beta}$-cyclodextrin.