• Title/Summary/Keyword: Inactive State

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Purification and Characterization of Superoxide Dismutase from Pseudomonas polycolor

  • LEE SANG-OK;IL-CHUN SEO;SOOK-HYUN CHUNG;TAE-HO LEE
    • Journal of Microbiology and Biotechnology
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    • v.3 no.3
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    • pp.188-193
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    • 1993
  • Superoxide dismutase (SOD) was purified from Pseudomonas polycolor to an electrophoretically homogeneous state and partially characterized. SOD was purified by ammonium sulfate fractionation, column chromatography on DEAE-Sephadex A-50, phenyl-Toyopearl 650 M, and gel filtration on Sephadex G-100. The molecular weight and subunit molecular weight of the purified enzyme were estimated to be 40, 000 and 20, 000, respectively. The purified enzyme remained stable at pH 9.0~11.0, $25^{\circ}C$ for 40 hr, but rapidly became inactive below 9.0. SOD was stable up to $45^{\circ}C$ at pH 9.0 with about 80% relative activity, but rapidly became inactive at temperature above that. The enzyme was insensitive to cyanide and fluoride, and sensitive to hydrogen peroxide and azide. The results suggest that the enzyme be an iron-containing SOD.

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Reorganization of Chromatin Conformation from an Active to an Inactive State After Cessation of Transcription

  • Lee, Myeong-Sok
    • Journal of Microbiology
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    • v.34 no.1
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    • pp.54-60
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    • 1996
  • Taking advantage of the heat inducible HSP82 gene in yeast, chromatin structure after transcription cessation was investigated. Alteration of chromating conformation within the HSP82 gene transcription unit into an active state has been shown to correlate with its transcriptional induction. It was thus of interest to examine whether the active chromatin state within the HSP82 mRNA analysis, the gene ceased its transcription within a few hours of cultivation at a normal condition after heat induction. In this condition, an active chromatin conformation in the HSP82 gene body was changed into an inactie state which was revealed by DNase I resistance and by typical nucleosomal cutting periodicity in the corresponding chromatin. These results thus ruled out the possibility of a long-term maintenance of the DNase I sensitive chromatin after transcription cessation. DNA replication may be a critical event for the chromatin reprogramming.

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STUDY OF SUPERHUMPS IN THE RECENTLY DISCOVERED SU UMA DWARF NOVAE

  • VOLOSHINA, I.;KHRUZINA, T.;METLOV, V.
    • Publications of The Korean Astronomical Society
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    • v.30 no.2
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    • pp.241-245
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    • 2015
  • In this work we present the results of light curve analysis for two cataclysmic variables detected recently in the SDSS project: SDSS J090350.73+330036.1 and J150240.98+333423.9. Photometric observations of the first were obtained during a superoutburst in May 2010. Our observations clearly indicate the presence of superhumps in the light curves, suggesting SDSS J090350.73+330036.1 is an SU UMa dwarf nova. We determined the period of the superhumps. We also carried out fitting using a spiral-arm model in order to determine parameters of the accretion disk, hot line, and other components of this system. Photometric observations of the second, J150240.98+333423.9, were obtained during the post-maximum decline, during April-June 2012. Photometric variability of this system has been studied in an inactive state. We obtained its parameters via a combined model fitted to the observed light curves by ${\chi}^2$ minimization.

Risk Factors for Breast Cancer in Postmenopausal Women in Brazil

  • Borghesan, Deise Helena Pelloso;Dell'Agnolo, Catia Millene;Gravena, Angela Andreia Francam;de Oliveira Demitto, Marcela;Lopes, Tiara Cristina Romeiro;de Barros Carvalho, Maria Dalva;Pelloso, Sandra Marisa
    • Asian Pacific Journal of Cancer Prevention
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    • v.17 no.7
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    • pp.3587-3593
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    • 2016
  • Purpose: To analyze risk factors for postmenopausal breast cancer. Methods: The present case-control study included 600 women treated at a cancer center reference hospital in a municipality in the South of Brazil. Results: Totals of 100 patients and 500 control subjects were evaluated. The mean age of the women was $52.5{\pm}11.9$ years; the average was $57.4{\pm}11.8$ years, and the average age of the control subjects was $51.5{\pm}11.7$ years. The risk factors for breast cancer that were considered included an age ${\geq}40$ years, postmenopausal status, a body mass index $(BMI){\geq}30kg/m^2$, and reduced physical activity. Variables like postmenopausal status and an obese BMI were associated with cases of breast cancer. Women who were postmenopausal or obese were 3.80 or 1.80 times more likely to develop breast cancer, respectively, and physically inactive women were 1.72 times more likely to develop breast cancer. Conclusions: Obesity and postmenopausal status are associated with the occurrence of breast cancer in this population. Being over 40 years of age was also a statistically significant factor for postmenopausal women.

The manufacturing Process Improvement usung Display of high VacuumExhausted Sealing Equipment by Tipless Manner (Tipless 방식에 의한 디스플레이 고진공 배기 밀봉장치 개발에관한 공정개발연구)

  • 김수용;박승곤;정원채
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2001.07a
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    • pp.817-820
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    • 2001
  • This machine is a high-vacuum exhaust sealing device which makes the inside of PDP element in high vacuum state, blows inactive gases into it and finally seals it. This machine consists of vacuuming parts, heating parts and exhausting parts. Applying the energy saving technology, this machine improves the temperature uniformity of vacuuming and heating parts.

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The manufacturing Improvement of high VacuumExhausted Sealing Equipment (고진공배기 시스템 장치개발에 관한연구)

  • Kim, Soo-Yong;Lee, Oh-Keol
    • Proceedings of the KIEE Conference
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    • 2001.07c
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    • pp.1795-1797
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    • 2001
  • This machine is a high-vacuum exhaust sealing device which makes the inside of PDP element in high vacuum state, blows inactive gases into it and finally seals it. This machine consists of vacuuming parts, heating parts and exhausting parts. Applying the energy saving technology, this machine improves the temperature uniformity of vacuuming and heating parts.

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A Technique for Full Wave Rectification using a Single Transistor (Single Transistor에 의한 전파정류 Technique)

  • 이주근;이동근
    • Journal of the Korean Institute of Telematics and Electronics
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    • v.15 no.3
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    • pp.7-10
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    • 1978
  • A method of full wave rectification is proposed which is accomplished by an inverter circuit including R-feed back. Both halves of the input cycle can be presented in the out put by composing a conductive half cycle and an inactive half cycle substituted by feed back in the cut off state.

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Slotted Transmission: A New MAC Scheme for Reduced Frame Latency in Ad-hoc Networks

  • Rahman, Md. Mustafizur;Hong, Choong-Seon
    • Proceedings of the Korea Information Processing Society Conference
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    • 2007.05a
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    • pp.1294-1296
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    • 2007
  • The IEEE 802.11 DCF forces neighboring nodes of an active transmitter to switch into inactive state. This conservative nature brings frame latency at transmitter neighborhood. This work exploits the IEEE 802.11n Frame Aggregation scheme to allow simultaneous transmissions from nodes that are neighbors to each-other. This is accomplished by the synchronization of control and data transmissions in slots of fixed length. Proposed scheme reduces the frame latency and improves aggregated network throughput.

The Effects of Meridian Massage on Behavioral State of Infants, Mothering Role Satisfaction and Mother-Infant Attachmen (경락마사지가 모성 애착과 역할 만족도 및 영아의 행동상태에 미치는 효과)

  • Cho, Kyoul-Ja;Lee, Myung-Hee;Ji, Eun-Sun
    • Child Health Nursing Research
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    • v.12 no.4
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    • pp.523-530
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    • 2006
  • Purpose: This study was to develop a nursing intervention for infants nursing. Meridian massage and to investigate its effects on the behavioral state of the infants, mothering role satisfaction and mother-infant attachment. Method: This study was conducted using a nonequivalent control group non-synchronized design in quasi experimental basis. Infants and mothers from Y health center were selected for the experimental group(17) and control group(14). Data were collected from August 11, 2005 to February 24, 2006. Infants in the experimental group were given Meridian massage education and practice for 50min three times a week for 3 weeks. Results: For behavioral state, when inactive infants in the experimental group showed a statistical significant difference by being in a greater state of alert as compared to the control group. The attachment of mother and infant was significantly higher in the experimental group than the control group. Satisfaction with mothering role was significantly higher in the experimental group than the control group. Conclusion: Meridian massage is an effective nursing intervention to improve positive behavioral state in infants and also in maternal-infant interaction.

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Expression and Characterization of RNA-dependent RNA Polymerase of Dendrolimus punctatus Tetravirus

  • Zhou, Liang;Zhang, Jiamin;Wang, Xiaochun;Jiang, Hong;Yi, Fuming;Hu, Yuanyang
    • BMB Reports
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    • v.39 no.5
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    • pp.571-577
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    • 2006
  • Dendrolimus punctatus tetravirus (DpTV) has been identified as a new member of the genus Omegatetravirus of the family Tetraviridae that may be related serologically to Nudaurelia capensis virus ($N{\omega}V$). To establish the function of DpTV RNA genome and to better understand the mechanism of viral replication, the putative RNA-dependent RNA polymerase (RdRp) domain has been cloned and expressed in Escherichia coli. The recombinant protein was purified on a Ni-chelating HisTrap affinity column and demonstrated to initiate viral RNA synthesis in a primer-independent manner but not by terminal nucleotidyle transferase activity in the presence of $Mg^{2+}$ and RNA template. Mutation of the GDD to GAA interferes with the residues at the polymerase active site and metal ions, and thus renders the polymerase inactive.