• Journal of Plant Biotechnology
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• v.35 no.1
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• pp.81-86
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• 2008

This study describes conditions for plant regeneration from protoplasts-derived from embryogenic callus of satsuma mandarin. Plants were generated via somatic embryogenesis. Protoplasts isolated directly from nucellar callus induced from immature ovule of satsuma mandarin cv. Okitsu (Citrus unshiu Marc.) were cultured in 0.6M $BH_3$ medium. Cell division and plating efficiency were affected by protoplast culture method. The liquid over solid method was the most effective for formation of microcalli. Most of microcalli grew rapidly and transferred onto embryoid formation medium. Optimum embryoid formation medium was MT medium containing 1.5 g/L malt extract, 0.146 M sucrose and the medium for plantlet regeneration was MS medium containing 0.09M sucrose, 1.0 mg/L $GA_3$. No differences were noticed in growth habits and leaf characters such as shape, thickness, and colour between protoplast-derived plants and nucellar seedlings. This plant regeneration system from protoplasts-derived from embryogenic callus provides an alternative way for producing new scion and rootstock cultivar from citrus species which can not be crossed.

• Journal of the Institute of Electronics Engineers of Korea TC
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• v.40 no.7
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• pp.293-299
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• 2003

In this paper, we have proposed a high conversion and isolation characteristic V-band quadruple sub-harmonic mixer monolithic circuit which is designed and fabricated for the millimeter wave down converter applications. While most of the sub-harmonic mixers use a half of fundamental frequency, we adopt a quarter of the fundamental frequency. The proposed circuit is based on a sub-harmonic mixer with APDP(anti-parallel diode pair) and the 0.1 ${\mu}{\textrm}{m}$ PHEMT's (pseudomorphic high electron mobility transistors). Lumped elements at IF port provide better selectivity of IF frequency and increase isolation. Maximum conversion gain of 0.8 ㏈ at a LO frequency of 14.5㎓ and at a RF frequency of 60.4 ㎓ is measured. Both LO-to-RF and LO-to-IF isolations are higher than 50 ㏈. The conversion gain and isolation characteristic are the best performances among the reported quadruple sub-harmonic mixer operating in the V-band millimeter wave frequency thus far.

• Herbal Formula Science
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• v.20 no.2
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• pp.83-92
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• 2012

Objectives : The aim of this study was to evaluate the efficacy of Hwangryunhaedok-tang (HHT) and Gungangbuja-tang (GBT) on lipopolysaccharide (LPS)-induced mouse model of inflammation. HHT and GBT are one of the representative prescriptions of cold drug and one of the representative prescriptions of hot drug, respectively. For experimental evaluation of their efficacy, we investigated the anti-inflammatory effects of HHT and GBT on LPS-induced inflammation and the mechanisms of their action. Methods : ICR mice were given a HHT (50, 500 mg/kg), GBT (100, 1000 mg/kg) extract orally on three consecutive days. On the third day, they were administered LPS intraperitoneally (35 mg/kg), 1 h after the last sample administration. Blood and liver samples were taken 6 h after the LPS challenge. Cytokine expression and inflammation-related protein factor analyses were performed by Western blotting. Results : Oral administration of HHT significantly reduced pro-inflammatory cytokines, including interleukin (IL)-6, and interferon (IFN)-${\gamma}$ in the serum. While GBT inhibited an increase of IL-6, IFN-${\gamma}$ was not affected. Immunoblot analysis showed that LPS-induced NF-${\kappa}b$ activation was inhibited by GBT, meanwhile HHT only inhibited NF-${\kappa}b$ expression at high does (500 mg/kg). In addition, HHT and GBT inhibited LPS-induced phosphorylation of Erk1/2, Jnk and p38 MAPKs. GBT also significantly inhibited i-Nos and Cox-2 expression, and HHT inhibited only i-Nos expression. Conclusions : Both of HHT and GBT showed anti-inflammatory effects against LPS-induced endotoxemia. However, HHT significantly decreased inflammatory cytokine levels, such as IL-6 and IFN-${\gamma}$ more than GBT, while GBT significantly inhibited inflammatory proteins, including NF-${\kappa}b$, MAP Kinases, i-Nos and Cox-2, more than HHT. These results suggest that HHT and GBT regulate the different mechanisms of action and pathways, presumably by regulating cytokine levels (IL-6, IFN-${\gamma}$), NF-${\kappa}b$ activation, and several pro-inflammatory gene expression, although both of HHT and GBT have anti-inflammatory effects.

• Journal of Korea Water Resources Association
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• v.44 no.12
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• pp.915-928
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• 2011

The main purpose of this study is to understand the effects of hydroclimatic factors on annual actual evapotranspiration and to suggest the multiple linear regression (MLR) equations for the estimation of annual actual evapotranspiration from watershed. To accomplish this study purpose, 5 dam watersheds (Goesan dam, Seomjingang dam, Soyanggang dam, Andong dam, Hapcheon dam) were selected as study watersheds and annual actual evapotranspiration was estimated based on annual water balance analysis from each watershed. The estimated annual actual evapotranspiration from water balance analysis was used to evaluate the MLR equations. Furthermore, the possibility of the estimation of actual evapotranspiration using potential evapotranspiration equations (Penman equation, FAO P-M equation, Makkink equation, Preistley-Taylor equation, Hargreaves equation) was evaluated. It has turned out that it is not appropriate to use potential evapotranspiration for the estimation of actual evapotranspiration because the correlation between actual evapotranspiration and potential evapotranspiration is very low. The comparison of MLR equations with current actual evapotranspiration equations indicates that MLR equations can be used for the estimation of annual actual evapotranspiration. Furthermore, it has turned out that the effects of hydroclimatic factors on annual actual evapotranspiration from dam watersheds are different in each watershed; however, for all watersheds in common precipitation has turned out to be the most important climatic factor affecting on the estimation of annual actual evapotranspiration.

• Fire Science and Engineering
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• v.22 no.1
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• pp.45-53
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• 2008

The effect of thinned trees which are produced from forest thinning on forest fire was studied in this work. To investigate the effect of thinning slash, Yang-yang, In-je, and Ga-pyeong-gun were selected as thinning-areas and non-thinning areas. The research was carried out with the variations of tree's types, area's characteristics, thinning strength, thinning types, and pile types of thinned tree. The survey areas of 14 areas were selected at Yangyang-gun(5 areas), Gapyeong-gun(4 areas), and Inje-gun(5 areas), and on-the-spot investigations were carried out at the thinning areas of 9 and the non-thinning areas of 5, respectively. Non-thinning areas of 5, which are adjacent to thinning areas, were selected for the comparison with thinning areas and for the analysis of risk of forest fire. It is considered that forest fire have no chance to diffuse to a tree trunk because the height of thinned trees was lower than 1 m. However, it is considered that forest fire may affect directly to a tree trunk if it spread to piled thinned tree because there was no space between thinned trees and trees. Furthermore, it was found that re-ignition had a chance to occur due to lots of piled thinning trees.

• Journal of the Korean Society of Food Science and Nutrition
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• v.43 no.7
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• pp.1036-1041
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• 2014

This study compared quality changes among brined baechu cabbages manufactured by various salting methods and types of salt. Brine, brine and dry salting, and dry salting were used as salting methods. When baechu cabbages were salted by dry salting method, the salinity of brined baechu cabbage increased compared to salting by other methods, even though the quantity of salt used was small. In addition, salinities of leaf and stem were relatively equal among brined baechu cabbages using dry salting method compared to those of other methods. When baechu cabbages were salted using dry method at different salt concentrations (3%, 5%, and 10% of weight of baechu), brined baechu cabbage showed suitable salinity (1.41~1.42%) at 5% salt concentration. Among brined baechu cabbages prepared using dry salting method with different types of salt (purified salt, solar salt, and bamboo salt), bamboo salt produced the highest salinity. Brined baechu cabbages with solar salt and bamboo salt showed significantly lower counts of total aerobic bacteria and higher counts of lactic acid bacteria than others. These results indicate that baechu cabbage can be salted equally, and the amount of salt used can be reduced when baechu cabbage is salted using dry salting method. In addition, using solar salt and bamboo salt can increase the quality of brined baechu cabbage.

• Journal of Life Science
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• v.23 no.4
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• pp.560-568
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• 2013

Chungkookjang was fermented by B. subtilis MC31, a ${\gamma}$-amino butyric acid (GABA) producing microorganism. The characteristics of Chungkookjang were investigated while fermenting. Twenty four amino acids were detected in Chungkookjang, leucine was the highest of them all. Total cell populations of B. subtilis MC31 phase were between log $9.52{\pm}0.5$ ~ log $9.049{\pm}0.5$ CFU/g at stationary phase. Contents of moisture, crude ash, crude protein, crude lipid and crude fiber are $61.07{\pm}0.01%$, $1.52{\pm}0.01%$, $17.66{\pm}0.04%$, $8.96{\pm}0.03%$ and 2.61%, respectively. Contents of ammonia type nitrogen, amino type nitrogen and reducing sugar were increased during fermentation at $40^{\circ}C$ for 72 hr, however those of titratable acidity and total sugar were decreased. pH was slowly alkalized during fermentation. Viscous substance and protease contents in Chungkookjang were $4.7{\pm}0.05%$ and $0.519{\pm}7.36$ g/l, apiece. When the fibrin plate and Robbin method for fibrinolytic activity were applied, B. subtilis MC31 showed high activity. These results suggested that B. subtilis MC31 is suitable to be used as a starter to enhance the quality of Chungkookjang.

• Korean Journal of Environment and Ecology
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• v.32 no.1
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• pp.55-65
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• 2018

This study was conducted to clarify diet items and predatory behavior of American bullfrog (Lithobates catesbeianus) according to the sex, maturity and season from April to September 2014 at Gahang wetland of Changnyeong-gun, Gyeongsangnamdo province, Korea. We examined the stomach contents of L. catesbeianus using a gastrectomy technique and identified the contents to a genus or species. The examination showed that large and heavy individual of L. catesbeianus fed on larger amounts of food. However, there were no statistically significant differences in predation amount according to the sex and maturity of L. catesbeianus. The main diet item of during the study period was mostly Insecta (average population rate of 65.5%), followed by Crustacea (13.5%) and Gastropoda (7.9%). The most preferred diet item of L. catesbeianus was Muljarus japonicus. Surprisingly, L. catesbeianus also foraged Parus major, Apodemus agrarius, and Crocidura lasiura. Ths findings showed that L. catesbeianus directly disturbed the wetland ecosystem. We expect the results will be the important reference data for checking the impact of L. catesbeianus, which is designated as invasive species by the Ministry of Environment, on wetland ecosystem.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.29 no.10A
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• pp.1223-1229
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• 2004

A variable-gain low-voltage low noise amplifier MMIC operating at 5GHz frequency band is designed and implemented using the ETRI 0.5$\mu\textrm{m}$ GaAs MESFET library process. This low noise amplifier is designed to have the variable gain for adaptive antenna array combined in HIPERLAN/2. The feedback circuit of a resistor and channel resistance controlled by the gate voltage of enhancement MESFET is proposed for the variable-gain low noise amplifier consisted of cascaded two stages. The fabricated variable gain amplifier exhibits 5.5GHz center frequency, 14.7dB small signal gain, 10.6dB input return loss, 10.7dB output return loss, 14.4dB variable gain, and 2.98dB noise figure at V$\_$DD/=1.5V, V$\_$GGl/=0.4V, and V$\_$GG2/=0.5V. This low noise amplifier also shows-19.7dBm input PldB, -10dBm IIP3, 52.6dB SFDR, and 9.5mW power consumption.

• Journal of Life Science
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• v.16 no.1
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• pp.114-119
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• 2006

The purpose of this study was to determine effects of the Ga-As (Gallium-Arsenide) Dens-Bio laser on mechanically injured sciatic nerves of rats. The improvement of the injured rat sciatic nerve was evaluated by measuring of nerve conduction velocity and amplitude of compound muscle action potential. The sciatic nerves of forty male Sprague-Dawley rats were compressed with hemostatic forceps for 30 seconds. The experimental group was divided into 4 subgroups according to the duration of treatment. Lower power infrared laser irradiation was done transcutaneously to the injured sciatic nerve area, 3 minutes daily to each of four treatment groups for 1, 3, 5, and 7 weeks, respectively. Compound muscle action potential and nerve conduction velocity of sciatic nerve were obtained before nerve injury and at 1, 3, 5, and 7 weeks after injury. There were significant difference of the nerve conduction velocity and amplitudes of compound muscle action potential between the treatment group and non-treatment group at 1, 3, and 5 weeks after laser treatment. However, there were no differences found between the electrophysiologic parameters that were measured after 7 weeks in two groups. There was significant correlation between the increment of compound muscle action potential and nerve conduction velocity after time course according to laser treatment. In conclusion, the low power laser treatment had improved the sciatic nerve function, and therefore these results may provide the basic data to clarify the neurological recovery and treatment after incomplete peripheral nerve injury.