Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
권호 표지
DOI QR코드

DOI QR Code

Electrophysiological Changes after Low-Power Infrared Laser Irradiation on Injured Rat Sciatic Nerves

손상된 흰쥐의 좌골신경에 저출력 레이저 조사후 전기생리학적 변화

  • Bae Chun-Sik (College of Veterinary Medicine, Biotechnology Research Institute, Chonnam National University) ;
  • Shin Soo-Beom (Department of Rehabilitation Medicine, College of Medicine, Chosun University) ;
  • Kim Kweon-Young (Department of Rehabilitation Medicine, College of Medicine, Chosun University)
  • 배춘식 (전남대학교 수의과대학 및 생물공학연구소) ;
  • 신수범 (조선대학교 의과대학 재활의학과) ;
  • 김권영 (조선대학교 의과대학 재활의학과)
  • Published : 2006.02.01
Download PDF
⟨ Previous Next ⟩

Abstract

The purpose of this study was to determine effects of the Ga-As (Gallium-Arsenide) Dens-Bio laser on mechanically injured sciatic nerves of rats. The improvement of the injured rat sciatic nerve was evaluated by measuring of nerve conduction velocity and amplitude of compound muscle action potential. The sciatic nerves of forty male Sprague-Dawley rats were compressed with hemostatic forceps for 30 seconds. The experimental group was divided into 4 subgroups according to the duration of treatment. Lower power infrared laser irradiation was done transcutaneously to the injured sciatic nerve area, 3 minutes daily to each of four treatment groups for 1, 3, 5, and 7 weeks, respectively. Compound muscle action potential and nerve conduction velocity of sciatic nerve were obtained before nerve injury and at 1, 3, 5, and 7 weeks after injury. There were significant difference of the nerve conduction velocity and amplitudes of compound muscle action potential between the treatment group and non-treatment group at 1, 3, and 5 weeks after laser treatment. However, there were no differences found between the electrophysiologic parameters that were measured after 7 weeks in two groups. There was significant correlation between the increment of compound muscle action potential and nerve conduction velocity after time course according to laser treatment. In conclusion, the low power laser treatment had improved the sciatic nerve function, and therefore these results may provide the basic data to clarify the neurological recovery and treatment after incomplete peripheral nerve injury.

저출력 적외선 레이저가 손상된 말초신경의 재생에 미치는 영향을 알아보기 위하여 흰쥐의 양측 좌골신경에 압궤손상을 준 뒤, 레이저 조사 기간에 따라 1, 3, 5 및 7주군으로 나누어 손상된 좌골신경의 신경전도속도와 진폭을 측정하여 신경재생의 정도를 관찰한 결과는 다음과 같았다. 좌골신경전도속도 검사에서 압궤손상 유발후에 손상전보다 유의하게 전도속도가 지연되었으나, 레이저를 조사한 실험측은 치료후 3주에 현저하게 신경전도 속도가 증가하였다. 좌골신경 진폭 검사에서 압궤손상 유발후에 손상전보다 유의하게 진폭이 감소하였으나, 레이저를 조사한 실험측은 치료후 3주에 복합근 활동전위의 진폭이 유의하게 증가하였다. 이상의 결과로 보아, 저출력 레이저 조사는 손상된 좌골신경의 기능회복에 있어, 주로 손상 초기의 회복속도에 영향을 주는 것으로 생각되는 바, 향후 말초신경 손상의 재활치료에 고려해야 할 것으로 생각된다.

Keywords

References

  1. Bae, C. S., S. C. Lim and S. C. Pak. 2002. Stimulatory effect of Ga-As infrared laser on the regeneration of injured sciatic nerves. J. Vet. Clin. 19, 316-321
  2. Chang, Y. U., S. S. Kim, S. W. Park, J. H. Son, K. E. Jang and D. S. Park. 2001. Electrophysiologic and Histologic Findings after Ethanol and Phenol Injection into the Sciatic Nerve of Rat. J. of Korean Acad. of Rehab. Med. 25, 69-78
  3. Cuadros, C. L. and C. E. Granatir. 1987. Nerve regeneration through a synthetic microporous tube (expanded polytetrafluoroethylene): Experimental study in the sciatic nerve of the rat. Microsurgery 8, 41-46 https://doi.org/10.1002/micr.1920080202
  4. Edward, M. S. B., J. E. Boggan and T. A. Fuller. 1983. The laser in neurosurgical surgery. J. Neurosurg. 59, 555-566 https://doi.org/10.3171/jns.1983.59.4.0555
  5. Gorio, A., P. Marini and R. Zanoni. 1983. Muscle reinnervation. III. Motoneuron sprouting capacity, enhancement by gangliosides. Neuroscience 8, 417-429 https://doi.org/10.1016/0306-4522(83)90189-6
  6. Gorio, A. and M. Vitadello. 1987. Ganglioside prevention of neuronal functional decay. pp. 289-325, In Seil, F. J., E. Herbert and B. M. Carlson (eds.), Progress in Brain Research, Vol 71, Elsevier, Amsterdam
  7. Horowitz, S. H. 1989. Therapeutic strategies in promoting peripheral nerve regeneration. Muscle Nerve 12, 314-322 https://doi.org/10.1002/mus.880120410
  8. Ito, H. and C. A. Bassett. 1983. Effect of weak, pulsing electromagnetic fields on neural regeneration in the rat. Clin. Orthop. Relat. Res. 181, 283-290
  9. Khullar, S. M., P. Brodin, E. B. Messelt and H. R. Haanes. 1995. The effects of low level laser treatment on recovery of nerve conduction and motor function after compression injury the rat sciatic nerve. Eur. J. Oral Sci. 103, 299-305 https://doi.org/10.1111/j.1600-0722.1995.tb00030.x
  10. Lazar, D. A., F. P. Curra, B. Mohr, L. D. McNutt, M. Kliot and P. D. Mourad. 2001. Acceleration of recovery after injury to the peripheral nervous system using ultrasound and other therapeutic modalities. Neurosurg. Clin. N. Am. 12, 353-357
  11. Mengs, U., K. F. Schmidt, H. G. Wolff, R. Goldschmidt and H. U. Tullner. 1984. Effects of gangliosides on nerve regeneration in rats. Arch. Int. Pharmacodyn. Ther. 271, 315-323
  12. Mester, E., T. Spiry, B. Szende and G. Tota. 1971. Effect of lasers on wound healing. Am. J. Surg. 122, 532-535 https://doi.org/10.1016/0002-9610(71)90482-X
  13. Oguzhanoglu, A., T. Kurt and T. Sahiner. 2000. Nerve conduction parameters during ischemia-reperfusion in the rat sciatic nerve. Electromyogr. Clin. Neurophysiol. 40, 487-490
  14. Pecot-Dechavassine, M. and J. C. Mira. 1994. Electrophysiological evaluation of the effects of naftidrofuryl on skeletal muscle reinnervation in the rat. Microsurgery 15, 116-122 https://doi.org/10.1002/micr.1920150206
  15. Power, S. K., J. E. Adams, M. S. B. Edwards, J. E. Boggan and Y. Hosobuchi. 1984. Pain relief from dorsal root entry zone lesions made with argon and carbon dioxide microsurgical lasers. J. Neurosurg. 61, 841-847 https://doi.org/10.3171/jns.1984.61.5.0841
  16. Ramon, Y. and S. Cajal. 1982. Degeneration and regeneration of the nervous system, pp. 228-229, Oxford University Press, London
  17. Rochkind, S., L. Barrnea, N. Razon, A. Bartal and M. Schwartz. 1987. Stimulatory effect of He-Ne low dose laser on injured sciatic nerves of rats. Neurosurgery 20, 843-847 https://doi.org/10.1227/00006123-198706000-00004
  18. Seddon, H. J. 1943. Three types of nerve injury. Brain 66, 237-288 https://doi.org/10.1093/brain/66.4.237
  19. Seddon, H. J. 1947. The use of autogenous grafts for the repair of large gaps in peripheral nerves. Br. J. Surg. 35, 151-167 https://doi.org/10.1002/bjs.18003513808
  20. Shi, K., R. Lu and X. Xu. 1997. Influence of low energy He-Ne laser on regeneration of peripheral nerve. Zhongguo. Xiu. Fu. Chong. Jian. Wai. Ke. Za. Zhi. 11, 14-18
  21. Tarlov, I. M. and J. A. Epstein. 1945. Nerve grafts: The importance of an adequate blood supply. J. Neurosurg. 2, 49-71 https://doi.org/10.3171/jns.1945.2.1.0049
  22. Varon, S. and R. P. Bunge. 1978. Trophic mechanism in the peripheral nervous system. Ann. Rev. Neurosic. 1, 327-361 https://doi.org/10.1146/annurev.ne.01.030178.001551
  23. Vita, G., R. Dattola and P. Girlanda. 1983. Effects of steroid hormones on muscle reinnervation after nerve crush in rabbit. Exp. Neurol. 80, 279-287 https://doi.org/10.1016/0014-4886(83)90282-0
  24. Wang, J. Z., D. C. Bai, C. M. Rao, W. G. Li, M. T. Gao, X. Q. Wang and Y. J. Wu. 1997. Effects of 2.5s mouse nerve growth factor on regeneration of injured sciatic nerves in mice and rats. Zhongguo. Yao. Li. Xue. Bao. 18, 501-504
  25. Wattig, B., G. Schalow, F. Heydenreich, R. Warzok and J. Cervos-Navarro. 1992. Enhancement of nerve fiber regeneration by nucleotides after peripheral nerve crush damage. Arzneimittelforschung 42, 1075-1078
  26. Wesselmann, U., J. M. Kerns and W. Z. Rymer. 1994. Laser effects on myelinated and nonmyelinated fibers in the rat peroneal nerve: A quantitative ultrastructural analysis. Exp. Neurol. 129, 257-265 https://doi.org/10.1006/exnr.1994.1168
  27. Zorko, B., J. Rozman and A. Seliskar. 2000. Influence of electrical stimulation on regeneration of the radial nerve in dogs. Acta. Vet. Hung. 48, 99-105 https://doi.org/10.1556/AVet.48.2000.1.11