Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Isolation and Identification of Histamine Degrading Bacteria from Kwamegi

과메기에서 histamine 분해능을 나타내는 세균의 분리 동정

  • Kim Min-Woo (Department of Food Science and Nutrition, Dong-eui University) ;
  • Kim Young-Man (Department of Food Science and Nutrition, Dong-eui University)
  • 김민우 (동의대학교 식품영양학과) ;
  • 김영만 (동의대학교 식품영양학과)
  • Published : 2006.02.01
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Abstract

To isolate and identify histamine degrading bacteria from Kwamegi, bacteria were screened with restriction media containing histamine. Ten strains were selected through morphological and biochemical identification procedure followed by comparison with DNA sequence of 16 rRNA gene. And also, these strains were confirmed by the histamine degrading assay such as turbidity and enzymatic assay. The results of identification are as followings : Ewingella americana B791, Arthrobacter sp. R45S, Halomonas marisflava, Psychrobacter sp. 9B-7, Bacillus sp. LMC 21002, Psychrohacter cibarius BC-220, Bacillus megaterium KL-197 were identified showing homology of $99\%,\;95\%,\;98\%,\;99\%,\;99\%,\;99\%\;and\;98\%$, respectively. Three strains remain unidentified. Arthrobacter sp. R45S, H. marisflava, Bacillus sp. LMG 21002, B. megaterium KL-197 showed histamine degrading activity, whereas, Psychrobacter sp. 9B-7 only showed weak activity. Three unidentified strains also have histamine degrading activity. In contrast, E. american B791 and p. cibarius JG-220 did not show any significant activity of histamine degradation. The strains isolated from this study showed relatively fast growth rate and histamine degrading rate as compared to those from salted mackerel.

과메기에서 histamine분해능을 가진 세균을 분리 동정하기 위하여 histamine만을 첨가한 제한배지에서 균을 분리했다. 기본적인 형태 및 생화학적 동정시험을 거쳐 10종의 시험균주를 선택하여 16S rDNA 염기서열 비교에 의한 계통발생학적 분석으로 동정하였다. 동정된 세균의 histamine분해능은 탁도측정법과 효소법에 의해 재 확인하였으며 그 실험 결과는 다음과 같다. 16S rDNA 염기서열 비교에 의한 계통 발생학적 분석을 이용하여 동정한 결과, E americana B791, Arthrobacter sp. R45S, H. marisflava, P. sp. 9B-7, Bacillus sp. LMG 21002, P. cibarius JG-220, B. megaterium KL-197 7종이 동정되었고, 각각 $99\%,\;95\%,\;98\%,\;99\%,\;99\%,\;99\%,\;98\%$의 상동성을 보였으며, 3종은 미동정되었다. 동정된 세균의 histamine분해능을 탁도측정법과 효소법에 의해 재 확인한 결과, Arthrobacter sp. R45S, H. marisflava, Bacillus sp. LMG 21002, B. megaterium KL-197이 분해능을 나타내었고, Psychrobacter sp. 9B-7은 미약한 분해능을 보였으며 미동정된 3종도 분해능이 있는 것으로 나타났다. 그리고 E. americana B791과 P. cibarius JG-220은 분해능이 불확실한 것으로 확인되었다 자반고등어에서 분리한 균주보다 시험균주 모두가 생육과 histamine의 분해속도가 비교적 빨랐다.

Keywords

References

  1. Yook, H. S., Y. J. Chung, H. P. Song, J. W. Lee and M. W. Byun. 2004. Genotoxicological safety of gamma-irradiated Kwamegi. J Korean Soc. Food. Sci. Nutr. 33, 182-192 https://doi.org/10.3746/jkfn.2004.33.1.182
  2. Hwang, S. J and Y. M. Kim. 2005. Isolation and Identification of a histamine-degrading bacteria from salted mackerel. Journal of Life Science 15, 743-748 https://doi.org/10.5352/JLS.2005.15.5.743
  3. Satake, K., S. Ando and H. Fugita. 1953. Bacterial oxidation of some primary amines. The Journal of biochemistry 49, 299-315
  4. Food and Drug Administration. 1992. Bacteriological analytical manual. 7th eds. AOAC International
  5. Holt, John G., Noel R., Krieg, Peter H. A. Sneath, James T. Staley and Stanley T. Williams. 1994. Bergey's manual of Determinative bacteriology. 9th eds. Baltimore, Williams ＆ Wilkins Co. 260-274
  6. Yoguchi, R., M. Okuzumi and T. Fujii. 1990. Seasonal variation in numbers of mesophilic and halophilic histamine-forming bacteria in inshore of Tokyo Bay and Sagami Bay. Nippon Suisan Gakkishi 56, 1467-1472 https://doi.org/10.2331/suisan.56.1467
  7. Moyer, C. L., F. C. Dobbs and D. M. Karl. 1994. Estimation of diversity and community structure through restriction fragment length polymorphism distribution analysis of bacterial 16S rRNA genes from a microbial mat at an active, hydrothermal vent system, Loiho Seamount, Hawaii, Appl. Environ. Microbiol. 60, 871-879
  8. Mullins, T. D., T. B. Britschgi, R. L. Krest and S. J. Giovannoni. 1995. Genetic comparison reveal the same unknown bacterial lineages in Atlantic and Pacific bacterioplankton communities, Limnol. Oceanigr. 40, 148-158 https://doi.org/10.4319/lo.1995.40.1.0148
  9. Wise, M. G., J. V. McARTHUR and L. J. Shimkets. 1997. Bacterial diversity of a Carolina Bay as determined by 16S rRNA gene analysis, conformation of novel taxa, Appl. Environ. Microbial. 63, 1505-1514
  10. Lee, T. C. 1992. Characterization and Utilization of the Amine Dehydrogenase and the Protease of Pseudomonas fluorescens P-3 Isolated from Salt-fermented Sardine, Sardinops melanosticta. Ph. D. Thesis, Kyungsung University, Busan, Korea
  11. Lerke, P. A., M. H. Porcuna and H. B. Chin. 1983. Screening test for histamine in fish. Journal of Food Science 48, 155-157 https://doi.org/10.1111/j.1365-2621.1983.tb14812.x
  12. Summer, S. S and S. L. Taylor. 1989. Detection method for histamine-protection dairy-related bacteria using diamine oxidase and leucocrystal violet. Journal of Food Protection 52, 105-108 https://doi.org/10.4315/0362-028X-52.2.105
  13. Grimont, P. A., J. J. Farmer 3rd, F. Grimont, M. A. Asbury, D. J. Brenner and C. Deval. 1983. Ewingella americana gen. nov., sp. nov., a new Enterobacteriaceae isolated from clinical specimens. Ann. Microbiol.(Paris) 134, 39-52
  14. Ryoo, N. H., S. H. Jung, D. S. Jeon, J. R. Kim and H. C. Kim. 2005. A Case of Pneumonia Caused by Ewingella americana in a Patient with Chronic Renal Failure. J Korean Med. Sci. 20, 143-145 https://doi.org/10.3346/jkms.2005.20.1.143
  15. Yoon, J. H., S. H. Choi, K. C. Lee, Y. H. Kho, K. H. Kang and Y. H. Park. 2001. Halomonas marisflavae sp. nov., a halophilic bacterium isolated from the Yellow Sea in Korea. International Journal of Systematic and Evolutionary Microbiology 51, 1171-1177 https://doi.org/10.1099/00207713-51-3-1171
  16. Juni, E and G. A. Heym. 1986. Psychrobacter immobilis gen. nov., sp. nov.: genospecies composed of gram-negative, aerobic, oxidase positive coccobacilli. Int. J. Syst. Bacteriol. 36, 388-391 https://doi.org/10.1099/00207713-36-3-388
  17. Bozal, N., M. J. Montes, E. Tudela and J. Guinea. 2003. Characterization of several Psychrobacter strains isolated from Antarctic environments and description of Psychrobacter Luti sp. nov. and Psychrobacter fozii sp. nov. International Journal of Systematic and Evolutionary Microbiology 53, 1093-1100 https://doi.org/10.1099/ijs.0.02457-0
  18. Jung, S. Y., M. H. Lee, T. K. Oh, Y. H. Park and J. H. Yoon. 2005. Psychrobacter cibarius sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. Int. J. Syst. Evol. Microbiol. 55, 577-582 https://doi.org/10.1099/ijs.0.63398-0
  19. Nekolny, D and J. Chaloupka. 2000. Protein catabolism in growing Bacillus megaterium during adaptation to salt stress. FEMS Microbiology Letters 184, 173-177 https://doi.org/10.1111/j.1574-6968.2000.tb09010.x
  20. Metcalfe, D. D. 1984. Food hypersensitivity. J. Allergy. Clin. Immunol. 73, pp. 749 https://doi.org/10.1016/0091-6749(84)90442-1
  21. Wantke, F., M. Gotz and R. Jarisch. 1993. The histamine-free diet. Hautarzt 44, 512
  22. Adams, E. J. 1992. Nutritional care in food allergy and food intolerance. In: Food, Nutrition and Diet Therapy. 8th eds. W. B. Saunders Company pp. 653
  23. Nam, H. W., K. W. Lee, C. O. Myung, J. S. Rhee, Y. C. Lee and C. S. Hong. Analysis on the contents of Histamine in Korean Foods. Korean J. Soc. Food Sci. 12, 489-491

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