• 대한의용생체공학회:의공학회지
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• 제28권1호
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• pp.95-101
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• 2007

Recently, small-animal imaging technology has been rapidly developed for longitudinal screening of laboratory animals such as mice and rats. One of newly developed imaging modalities for small animals is an x-ray micro-CT (computed tomography). We have developed two types of x-ray micro-CT systems for small animal imaging. Both systems use flat-panel x-ray detectors and micro-focus x-ray sources to obtain high spatial resolution of $10{\mu}m$. In spite of the relatively large field-of-view (FOV) of flat-panel detectors, the spatial resolution in the whole-body imaging of rats should be sacrificed down to the order of $100{\mu}m$ due to the limited number of x-ray detector pixels. Though the spatial resolution of cone-beam CTs can be improved by moving an object toward an x-ray source, the FOV should be reduced and the object size is also limited. To overcome the limitation of the object size and resolution, we introduce zoom-in micro-tomography for high-resolution imaging of a local region-of-interest (ROI) inside a large object. For zoom-in imaging, we use two kinds of projection data in combination, one from a full FOV scan of the whole object and the other from a limited FOV scan of the ROI. Both of our micro-CT systems have zoom-in micro-tomography capability. One of both is a micro-CT system with a fixed gantry mounted with an x-ray source and a detector. An imaged object is laid on a rotating table between a source and a detector. The other micro-CT system has a rotating gantry with a fixed object table, which makes whole scans without rotating an object. In this paper, we report the results of in vivo small animal study using the developed micro-CTs.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 2005년도 춘계학술대회 논문집
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• pp.892-895
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• 2005

This study investigated changes of mechanical characteristics in the trabecular and cortical bone of the lumbar vertebrae of the ovariectomised(OVX) rat. In previous researches, there were many studies for morphology of osteoporotic bones based on Micro-CT (Micro - Computed Tomography). However, there were few studies for detecting and tracking changes of mechanical characteristics in the trabecular bone and cortical bone of the lumbar vertebrae for the OVX rat. For this study, one female Sprague-Dawley rat was used: an OVX rat. The 4th lumbar of the OVX rat was utilized as a specimen. Detecting and tracking changes of mechanical characteristics could be investigated in the trabecular and cortical bone of the lumbar vertebrae for an OVX rat using In-vivo Micro-CT. An OVX rat was scanned at week 0 (just before surgery), at week 4, and week 8 after surgery. Micro finite element(${\mu}\;FE$) analysis was used to investigate mechanical characteristics in the trabecular and cortical bone of the lumbar vertebrae for an OVX rat.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 2010년도 춘계학술대회 논문집
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• pp.1359-1360
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• 2010

• 한국정밀공학회지
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• 제24권1호
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• pp.110-117
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• 2007

This study investigated changes of mechanical characteristics of the trabecular and cortical bone in the lumbar vertebrae of the ovariectomised (OVX) rat. In previous researches, there were many studies for morphology of osteoporotic bones based on Micro-Computed Tomography (Micro-CT). However, there were few studies for detecting and tracking changes of mechanical characteristics of the trabecular and cortical bone in the lumbar vertebrae of the OVX rat. For this study, the 4th lumbar of the OVX rat (female Sprague-Dawley) was utilized as a specimen. An OVX rat was scanned at week 0 (just before surgery), at week 4, and week 8 after surgery. Micro-finite element (${\mu}-FE$) analysis was used to investigate mechanical characteristics of the trabecular and cortical bone in the lumbar vertebrae for an OVX rat. When the OVX rat (at week 8) was compared with the OVX rat (at week 0), the structural modulus of cortical and trabecualr bone was decreased by 52% and 99%, respectively. This study showed the change of mechanical characteristics of cortical bone as well as trabecular bone of an OVX rat. Detecting and tracking changes of mechanical characteristics could greatly contribute to an experiment test for the trabecular and cortical bone in the lumbar vertebrae of an OVX rat by using In-vivo Micro-CT.

• Imaging Science in Dentistry
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• 제38권1호
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• pp.29-37
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• 2008

Purpose: The aim of this study was to observe the bony change in the OVX rat longitudinally and to study the alendronate effect. Materials and Methods: Eighteen Sprague-Dewley rats, eight-week old each, were randomly assigned into three groups: one of those sham-operated (N=4), the other two were OVX: saline-treated (N=7) and alendronate-treated group (N=7). The saline-treated group was administered with saline solution (0.1mL/100g) daily, while the alendronate-treated group was given alendronate (1mg/kg, Sigma-Aldrich Corp. Korea) daily. Micro-CT scannings of the lumbar were consecutively done at baseline, at 3-week intervals during 9 weeks. Two and three dimensional bony analysis were done. Bone mineral density (BMD) was measured with Piximus (GE Lunar Co. USA). The average values of these three methods were compared with each group. Results: After 6 weeks the BMD of the OVX group showed lower tendency than that of sham group. After 6 weeks many 3D parameters of micro-CT showed higher values in the OVX-alendronate group compared with the OVXsaline group. Most 2D bony parameters were higher in the OVX-alendronate group compared with the OVX-saline group at 9 weeks. Conclusion: This study showed low BMD of the OVX group after 6 weeks and showed the effect of alendronate on the BMD and bony structures of ovariectomized rats. This study also showed usefulness of in vivo micro-CT in monitoring individual bone changes over time.

• Journal of Periodontal and Implant Science
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• 제47권1호
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• pp.30-40
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• 2017

Purpose: Optical coherence tomography (OCT) is a noninvasive diagnostic technique that may be useful for both qualitative and quantitative analyses of the periodontium. Micro-computed tomography (micro-CT) is another noninvasive imaging technique capable of providing submicron spatial resolution. The purpose of this study was to present periodontal images obtained using ex vivo dental OCT and to compare OCT images with micro-CT images and histologic sections. Methods: Images of ex vivo canine periodontal structures were obtained using OCT. Biologic depth measurements made using OCT were compared to measurements made on histologic sections prepared from the same sites. Visual comparisons were made among OCT, micro-CT, and histologic sections to evaluate whether anatomical details were accurately revealed by OCT. Results: The periodontal tissue contour, gingival sulcus, and the presence of supragingival and subgingival calculus could be visualized using OCT. OCT was able to depict the surface topography of the dentogingival complex with higher resolution than micro-CT, but the imaging depth was typically limited to 1.2-1.5 mm. Biologic depth measurements made using OCT were a mean of 0.51 mm shallower than the histologic measurements. Conclusions: Dental OCT as used in this study was able to generate high-resolution, cross-sectional images of the superficial portions of periodontal structures. Improvements in imaging depth and the development of an intraoral sensor are likely to make OCT a useful technique for periodontal applications.

• 한국컴퓨터정보학회논문지
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• 제12권2호
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• pp.123-130
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• 2007

본 논문에서는 소동물 생체내 실험시 서로 다른 장비에서 획득된 영상의 융합 및 정합을 위한 방법을 제안한다. 마우스의 꼬리 정맥에 $[[^{18}F]FDG$를 주사하여 60분 섭취후 서로 다른 장비에서 동일한 위치의 영상을 획득하기 위하여 아크릴 재질의 소동물 가이드에 기준마크를 설정하고 microPET과 CT 영상을 획득하였다. MicroPET으로 획득된 리스트모드(list-mode) 데이터는 Fourier Rebinning(FRB) 방법을 사용하여 사이노그램(Sinogram)으로 변환 후 4 번의 반복횟수를 가지는 Ordered Subset Expectation Maximization(OSEM) 알고리즘으로 재구성하였다. MicroPET 영상획득후 PET/CT의 CT를 이용하여 CT영상을 획득하였다. MicroPET 영상에서 폐영역을 정확히 찾아내는 어려움이 있어. 해부학적 정보를 제공하는 CT 영상을 이용하여 폐 영역을 구분하였다. 영상 융합을 위한 불일치 부분을 해결하기 위하여 기준마크의 정보와 폐 영역의 정보를 이용하여 회전과 이동정보를 가지는 어파인 (affine) 변환 행렬 구하여 영상 정합에 사용하였다. 이 방법은 정량적 정확성과 영상 해석의 정확성을 개선할 것으로 기대된다.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 2005년도 추계학술대회 논문집
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• pp.236-239
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• 2005

This study investigated the effect of ovariectomy (OVX) on the rat bone fur long term (22 weeks). In previous researches, there were many studies for morphology of OVX-induced osteoporotic bones based on micro-Computed Tomography (micro-CT). However, there were few studies fpr detecting and tracking changes of mechanical characteristics in the lumbar vertebrae of OVX rat fur long-term. For this study, one female Sprague-Dawley rat was used: an OVX rat. The 4th lumbar of the OVX rat was utilized as a specimen. Morphological characteristics could be investigated fur the lumbar vertebrae in an OVX rat by using in-vivo Micro-CT. An OVX rat was scanned at week 0 (just before surgery), at week 4, at week 8, at week 16 and at week 22 after surgery. Micro finite element $({\mu}FE)$ analysis was used to investigate mechanical characteristics in the lumbar vertebrae for an OVX rat.

• 대한약리학회:학술대회논문집
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• 대한약리학회 2006년도 58th Annual Meeting of The Korean Society of Pharmacology
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• pp.54-54
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• 2006

• 대한의용생체공학회:의공학회지
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• 제33권4호
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• pp.213-222
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• 2012

While much has been learned about the mechanisms of metastatic spread of cancer to bone, there has been little headway in establishing guidelines for monitoring the alteration in bone quality and estimating fracture risk. The aims of this study are, therefore, 1) to evaluate bone quality induced by metastatic bone tumor by analyzing the characteristics on bone microarchitecture and degree of bone mineralization and 2) analyze fracture risk increased secondary to the bone quality changes by metastatic bone tumor through calculating mechanical rigidities based on in-vivo micro CT images. For this study, eighteen female SD rats (12 weeks old, approximate 250 g) were randomly allocated in Sham and Tumor groups. W256 (Walker carcinosarcoma 256 malignant breast cancer cell) was inoculated in the right femur (intraosseous injection) in Tumor group, while 0.9% NaCl (saline solution) was injected in Sham group. The right hind limbs of all rats were scanned by in-vivo micro-CT to acquire structural parameters and degree of bone mineralization at 0 week, 4 weeks, 8 weeks, and 12 weeks after surgery. At the same time, urine was collected by metabolic cages for a biochemical marker test in order to evaluate bone resorption. Then, bone metastasis had been directly identified by positron emission tomography. Finally, axial, bending and torsional rigidities had been calculated based on in-vivo micro CT images for predict fracture risk. The results of this study showed that metastatic bone tumor might induce significant decrease in bone quality and increase of fracture risk. This study may be helpful to monitoring a degree of bone metastasis and predicting fracture risk due to metastatic bone tumor. In addition, this noninvasive diagnostic methodology may be utilized for evaluating other bone metabolic diseases such as osteoporosis.