• 한국수정란이식학회지
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• 제15권1호
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• pp.85-94
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• 2000

These experiments were conducted to examine the effects of theophylline, pentoxifylline and heparin on frozen-thawed Hanwoo sperm for enhancing motility and viability of sperm. Frozen-thawed semen collected from one bull was treated in TALP(tyrode-albuminlactate-pyruvate) containing varous concentrations of theophylline and pentoxifylline. After incubated at 5% CO2 in air atmosphere for 6 hours, the motility of sperm after the treatments was characterized by CASA(computer aided semen analysis) system. When monitored notility(MOT) and curvilinear velocity(VCL), theophylline and pentoxifylline exerted their optimal action at the concentration of 30 mM and 3 mM, respectively. No difference of sperm motility was observed when the sperm was treated with both substances compared with a single treatment of each substance. Comparison was then made for evaluating the effect of theophylline and / or pentoxiophylline on the motility and viability of significant treatment effects of each substance, high MOT and VCL values were detected in sperm treated with theophylline. In the case of sperm viability examined by an eosin-nigrosin staining, however, a significant decrease was found after the combined treatment of theophylline+pentoxyphilline than after the treatment with heparin alone or no treatment(P<0.05). In conclusion, theophylline, pentoxiphylline or heparin can be used for enhancing the motional characteristics and viability of frozen thawed Hanwoo semen. Considering characteristics of these substances, theophyline may be useful in the artificial insemination system, which requires vigorous sperm motility. While, heparin supporting sperm viability in vitro can be effectively used for improving in vitro-fertilization system.

• KSBB Journal
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• 제19권5호
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• pp.341-347
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• 2004

Several gene delivery therapies are being developed for treatment of serum protein deficiency. EPO is one of the most promising therapeutic agent for this treatment which is currently being investigated in depth. This study has the ultimate purpose of improving the gene delivery system for an increase of red blood cell production. A plasmid DNA was constructed smaller than other plasmids for an increase in penetration into animal cells, and two genes were cloned into each vector as a co-delivery system to express erythropoietin, and interluekin-3 or thrombopoietin, which can act on erythroid cell, thus activating hematopoiesis synergically. This co-delivery system has an advantage of decreasing the labour required for industrial production of DNA vaccine. A new plasmid vector, pVAC, in size 2.9 kb, was constructed with the essential parts from PUC 19 and pSectagB, which is much smaller than other plasmid vector and is the size of 2.9 kb. Co-delivery system was constituted by cloning human erythropoietin with each of human interluekin-3 gene or human thrombopoietin gene into both pVAC and pSectagB. As a result, the transfection efficiency of pVAC was higer than that of pSectagB in vitro, and hematocrit level of the mice injected with pVAC is higher than that of other mice. And co-delivery system, made of several plasmid DNAs, was expressed in vitro.

• Biomaterials and Biomechanics in Bioengineering
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• 제2권3호
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• pp.143-157
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• 2015

Successful integration of cementless femoral stems using porous surfaces relies on effective periimplant bone healing to secure the bone-implant interface. The initial stages of the healing process involve protein adsorption, fibrin clot formation and cell osteoconduction onto the implant surface. Modelling this process in vitro, the current work considered the effect of fibrin deposition on the responses of human mesenchymal stromal cells cultured on ferritic fibre networks intended for magneto-mechanical actuation of in-growing bone tissue. The underlying hypothesis for the study was that fibrin deposition would support early stromal cell attachment and physiological functions within the optimal regions for strain transmission to the cells in the fibre networks. Highly porous fibre networks composed of 444 ferritic stainless steel were selected due to their ability to support human osteoblasts and mesenchymal stromal cells without inducing untoward inflammatory responses in vitro. Cell attachment, proliferation, metabolic activity, differentiation and penetration into the ferritic fibre networks were examined for one week. For all fibrin-containing samples, cells were observed on and between the metal fibres, supported by the deposited fibrin, while cells on fibrin-free fibre networks (control surface) attached only onto fibre surfaces and junctions. Initial cell attachment, measured by analysis of deoxyribonucleic acid, increased significantly with increasing fibrinogen concentration within the physiological range. Despite higher cell numbers on fibrin-containing samples, similar metabolic activities to control surfaces were observed, which significantly increased for all samples over the duration of the study. It is concluded that fibrin deposition can support the early attachment of viable mesenchymal stromal cells within the inter-fibre spaces of fibre networks intended for magneto-mechanical strain transduction to in-growing cells.

• Parasites, Hosts and Diseases
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• 제57권1호
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• pp.1-8
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• 2019

There is no effective treatment modality available against different forms of leishmaniasis. Therefore, the aim of this study was to improve the penetration and efficacy of selenium and glucantime coupled with niosomes and compared them with their simple forms alone on in vitro susceptibility assays. In this study, the niosomal formulations of selenium and in combination with glucantime were prepared. The size and morphology of the niosomal formulations were characterized and the effectivity of the new formulation was also evaluated using in vitro MTT assay, intra-macrophage model, and gene expression profile. From the results obtained, no cytotoxicity effect was observed for niosomal and simple forms of drugs, as alone or in combination. Niosomal formulations of the drugs significantly showed more inhibitory effects ($P{\leq}0.001$) than the simple drugs when the selectivity index was considered. The gene expression levels of Interleukin (IL-10) significantly decreased, while the level of IL-12 and metacaspase significantly increased ($P{\leq}0.001$). The results of the present study showed that selenium plus glucantime niosome possess a potent anti-leishmanial effect and enhanced their lethal activity as evidenced by the in vitro experiments.

• Clinical and Experimental Reproductive Medicine
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• 제18권1호
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• pp.63-71
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• 1991

To establish the normal fertile range in the results of the sperm zona-free hamster ova penetration assay (SPA) in Korean male, SPA using the low temperature ($4^{\circ}C$) capacitation in TEST-yolk buffer (TYB) was performed in 67 fertile and 26 infertile men. Sperm parameters in routine semen analysis were also checked and compared with the results of SPA. Sperm concentration, motility and motility index (MI) were significantly higher in fertile group compared with infertile group: $96.0{\pm}46.6$ vs $43.6{\pm}31.9{\times}10^6/ml$, $65.5{\pm}14.8%$ vs $45.8{\pm}23.6%$ and $46.31{\pm}13.29$ vs 27.40{\pm}17.98$, respectively. In fertile group, the hamster ova penetration rate (PR) was $98.5{\pm}5.0%$ (80%-100%), and the penetration index (mean penetrations per ovum, PI) was $9.59{\pm}6.35$(3.1-29.0). All the fertile men showed PI>3.0. In infertile group, PR was $24.6{\pm}24.8%$ (0%-70%), and PI was $0.40{\pm}0.42$ (0-1.3). Both PR and PI were significantly lower in infertile group. There was a significant correlation beween PI and sperm motility or MI, respectively, in fertile group whereas there was no correlation in infertile group. These data suggest that SPA using the low temperature capacitation in TYB can be a valuable diagnostic tool for the assessment of male fertility in vitro and provide an important supplement to the traditional tests of sperm quality.

• 한국가축번식학회지
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• 제9권2호
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• pp.148-152
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• 1985

When goat spermatozoa were preincubated for 4-6 h and 6 h in the uteri isolated from hamster and rat, and for 6 h in the hamster uterus in situ, they developed the ability to penetrate zona-free hamster eggs in vitro. Zona-free hamster eggs were not penetrated after insemination with goat spermatozoa preincubated in the isolated hamster uterus 4 h before and 2 h after expected time of ovulation, respectively. Zona-free hamster eggs were not penetrated after insemination with goat spermatozoa preincubated for 4 h in the isolated hamster uterus, but 10 and 18% of eggs were penetrated by spermatozoa preincubated for 5 and 6 h in the isolated uterus.

• 한국환경보건학회지
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• 제27권4호
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• pp.73-78
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• 2001

Hair will be persuit of beautifulness of human being in various permanent wave by many kinds of its drugs. Skin is based upon the hair which enroll the body of high living animals and have multiple membrane structure In this study used rat the effects of commercial permanent wave products to skin which are composed with L- cysteine and bases Results are as follow: the content of penetration 4 hors later with steady state and no significant changeable after 20 hours later. In cysteine groups, lag time and permeability coefficient of healthy skin is 2.22hr and 0.13$\mu\textrm{g}$/㎥ hr, lag time and permeability coefficient of old skin is 4.01 hr and 0.108 $\mu\textrm{g}$/㎥ hr . In conclusion of study lag time and permeability coefficient in old skin and wounded skin are faster than healthy skin. We notified that fine rinkle and rash of skin were changeable in the case of treating with permanent wave drugs than normal skin.

• 대한소아치과학회지
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• 제44권3호
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• pp.272-279
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• 2017

이 연구의 목적은 2종의 친수성 치면열구전색제를 건조한 법랑질과 습한 법랑질에 적용하였을 때의 미세누출과 열구 침투도를 평가하는 것으로, 기존의 소수성 전색제인 $Clinpro^{TM}$와 비교하였다. 건전하고 우식이 없는 제3대구치 60개를 법랑질 수분 처리 방법과 전색제 종류에 따라 12개씩 다섯 군으로 나누었다. 전색제 적용 후 치아들을 열순환 시킨 뒤 1% 메틸렌블루 용액에 담갔다. 그리고 치아들을 두 번씩 절단한 뒤 절단면들을 광학 현미경과 이미지 분석 프로그램을 이용해 관찰하였다. Embrace $Wetbond^{TM}$는 건조한 표면과 습한 표면 모두에서 $Clinpro^{TM}$보다 많은 미세누출을 나타냈다. Ultraseal $XT^{(R)}$ $hydro^{TM}$는 건조한 표면에서는 $Clinpro^{TM}$와 유사한 미세누출을 보였으나, 습한 표면에서는 많은 미세누출을 보였다. 열구 침투도는 모든 군에서 통계적으로 유의한 차이가 없었다. 결론적으로 친수성 전색제들을 습한 법랑질에 적용했을 때 변연 봉쇄성이 향상되지 않았으며, 건조한 표면에 적용한 $Clinpro^{TM}$보다 낮은 변연 봉쇄성이 관찰되었다.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2005년도 생물공학의 동향(XVII)
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• pp.816-820
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• 2005

Polysaccharide-based dressings have increasingly become viable alternatives to somewhat less biocompatible and often problematic cotton or viscose gauzes traditionally used for wound dressings. Polysaccharide which is particularly abundunt in Agaricus blazei Murill is known as the bioactive materials. Polysaccharide extracted from Agaricus blazei Murill enhances the recovery of the damaged epidermal tissue and the production of epithelial cell growth factors which are necessary to heal the burn and wound on the epidermal tissue. In this study, the biopolymer delivery system of polysaccharide extracted from Agaricus blazei Murill into epidermal tissue was elucidated using the skin permeating enhancer in vitro. The enhancing effects of various penetration enhancers and some other enhancers on the permeation of ${\beta}-glucan$ were evaluated using Franz diffusion cell. Permeation of polysaccharide in nomal and burn skin was compared. Polysaccharide was studied for the burn and wound healing activity in the epidermal tissue on rats.

• Restorative Dentistry and Endodontics
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• 제18권2호
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• pp.497-500
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• 1993

Sealapex was compaired with a zinc oxide eugenol sealer over a 24 - wk interval to examine solubility in an in vitro situation. After obturation with gutta percha and the appropriate sealer, speciemens were immediately immersed in a saline solution to challenge the solubility of the sealers. The solutions were changed weekly to allow for a continued dissolution of the sealers. After immersed in methylene blue solution for 3 days, the speciemens were made transparent by a clearing process. Microscopic examination was used to determine the linear penetration of dye for each speciemen. Result revealed that sealapex had no greater dissolution than ZOE(P<0.05).