• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.10 no.1
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• pp.31-37
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• 2005

To find out temporal variations of net photosynthetic rate (NPR) of intertidal flats, we measured oxygen microprofiles in sediments with oxygen microsensors 4 times from December 2003 to June 2004. The study areas were the intertidial flats in Janghwa-ri and Dongmak-ri, located on the southwestern and the southern parts of Ganghwa-gun, respectively, and in Incheon North Harbor where the content of organic matter was relatively high. During the investigation, oxygen penetration depths in the tidal flats of Janghwa-ri and Dongmak-ri were high in December (mean values of 4.0-4.1 mm). Thereafter, the oxygen penetration depths declined to mean values of 2.2-2.8 mm and 1.6-1.8 mm in the two tidal flats. Interestingly, the oxygen penetration depths in the Incheon North Harbor tidal flat showed a lower range $(0.8{\pm}0.3\;mm;\;mean{\pm}1SD)$ over the period. The maximum NPR in the Dongmak-ri tidal flat was found in March $(11.1{\pm}2.8\;mmol\;O_2\;m^{-2}\;h^{-1})$, and those In Janghwa-ri $(6.1{\pm}4.1\;mmol\;O_2\;m^{-2}\;h^{-1})$ and Incheon North Harbor $(6.4{\pm}1.4\;mmol\;O_2\;m^{-2}\;h^{-1})$ were observed in May. During the period when NPR was most active, the highest oxygen concentration was found at 0.1-0.5 mm depth below the surface sediment, and was on average 1.8-3.2 times higher than the air-saturated oxygen concentration in the overlying seawater. Although we took into account of low in situ light intensity $(400{\mu}Einst\;m^{-2}\;s^{-1})$ during the investigation in June, NPR in the 3 study areas decreased significantly to less than $0.2\;mmol\;O_2\;m^{-2}\;h^{-1})$. Thus, temporal variations of NPR were somewhat different among the tidal flats. Generally, benthic primary producers inhabiting in the uppermost 0.5 mm of the sediment showed a peak photosynthetic activity in the study areas in spring. This is the first domestic report on photosynthetic rates of benthic microflora in the tidal flats with oxygen microsensors, and the use of the microsensor can be widely applied to measurements of benthic primary production of a tidal flat and the oxygen consumption rate of surficial sediments.

• Journal of Chest Surgery
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• v.32 no.7
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• pp.637-647
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• 1999

Background: Ischemia-reperfusion injury is one of the major contributing causes of early graft failure in lung transplantation. It has been suggested that triiodothyronine (T3) may ameliorate ischemia-reperfusion injury to various organs in vivo and in vitro. Predicting its beneficial effect for ischemic lung injury, we set out to demonstrate it by administering T3 into the in situ canine ischemia-reperfusion model. Material and Method: Sixteen adult mongrel dogs were randomly allocated into group A and B. T3 $(3.6\mug/kg)$ was administered before the initiation of single lung ischemia in group B, whereas the same amount of saline was administered in group A. Ischemia was induced in the left lung by clamping the left hilum for 100 minutes. After reperfusion, various hemodynamic parameters and blood gases were analyzed for 4 hours while intermittently clamping the right hilum in order to allow observation of the injured left lung function. Result: Arterial oxygen partial pressure $(PaO_2)$ decreased 30 minutes after reperfusion and recovered gradually thereafter in both groups. In group B the decrease of $PaO_2$ was less marked than in group A. The recovery of $PaO_2$ was faster in group B than in group A. The differences between the two groups were statistically significant from 30 minutes after reperfusion $(125\pm34$ mmHg and $252\pm44$ mmHg, p<0.05) until the end of the experiment $(178\pm42$mmHg and $330\pm37$ mmHg, p<0.05). The differences in the arterial carbon dioxide pressure, airway pressure and lung compliance showed no statistical significance. The malondialdehyde (MDA) level, measured from the tissue obtained 240 minutes after reperfusion, was lower in group B $(0.40\pm0.04\mu$M) than in group A $(0.53\pm0.05\mu$M, p<0.05). The ATP level of group B $(0.69\pm0.07\mu$M/g) was significantly higher than that of group A $(0.48\pm0.07\mu$M/g, p<0.05). The microscopic exami nation revealed varying degrees of injury such as perivascular neutrophil infiltration, capillary hemorrhage and interstitial congestion. There were no differences in the microscopic findings between the two groups. CONCLUSION T3 has beneficial effects on the ischemic canine lung injury including preservation of oxygenation capacity, less production of lipid peroxidation products and a higher level of tissue ATP. These results suggest that T3 is effective in pulmonary allograft preservation.

• Asian Pacific Journal of Cancer Prevention
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• v.17 no.4
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• pp.2159-2164
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• 2016

Background: A diagnosis of chronic myeloid leukemia (CML) is made on discovery of the presence of a Philadelphia (Ph) chromosome. The success of the treatment of this form of leukemia with tyrosine kinase inhibitor (TKI) is monitored by reduction of the Ph chromosome. Objective: To compare the role of conventional cytogenetic (CC) methods with a real time quantitative polymerase chain reaction (RQ-PCR) and fluorescence in situ hybridization (FISH) for diagnosis and treatment monitoring of CML patients. The secondary outcome was to analyze the treatment responses to TKI in CML patients. Materials and Methods: This was a retrospective study of CML patients who attended the Hematology clinic at Chiang Mai University Hospital from 2005-2010. Medical records were reviewed for demographic data, risk score, treatment response and the results of CC methods, FISH and RQ-PCR. Results: One hundred and twenty three cases were included in the study, 57.7% of whom were male with a mean age of 46.9 years. Most of the patients registered as intermediate to high risk on the Sokal score. At diagnosis, 121 patients were tested using the CC method and 118 (95.9%) were identified as positive. Five patients failed to be diagnosed by CC methods but were positive for BCR-ABL1 using the FISH method. Imatinib was the first-line treatment used in 120 patients (97.6%). In most patients (108 out of 122, 88.5%), a complete cytogenetic response (CCyR) was achieved after TKI therapy and in 86 patients (70.5%) CCyR was achieved long term by the CC method. Five out of the 35 analyzed patients in which CCyR was achieved by the CC method had a positive FISH result. Out of the 76 patients in which CCyR was achieved, RQ-PCR classified patients to only CCyR in 17 patients (22.4%) with a deeper major molecular response (MMR) in 4 patients (5.3%) and complete molecular response (CMR) in 55 patients (72.4%). In the case of initial therapy, CCyR was achieved in 95 patients (79.1%) who received imatinib and in both patients who received dasatinib (100%). For the second line treatment, nilotinib were used in 30 patients and in 19 of them (63.3%) CCyR was achieved. In half of the 6 patients (50%) who received dasatinib as second line or third line treatment CCyR was also achieved. Conclusions: CML patients had a good response to TKI treatment. FISH could be useful for diagnosis in cases where CC analysis failed to detect the Ph chromosome. RQ-PCR was helpful in detecting any residual disease and determining the depth of the treatment response at levels greater than the CC methods.

• Journal of Korean Society of Forest Science
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• v.109 no.3
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• pp.271-280
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• 2020

This study compared soil CO₂ efflux rates after fertilization, in Pinus densiflora and Quercus variabilis stands. Compound fertilizers were applied to the forest floor in March 2016, following a one-year calibration period (from March 2015 to February 2016). In situ soil CO₂ efflux rates were measured every month during the two-year study periods, using an infrared gas analyzer with a closed chamber system. Mean annual soil CO₂ efflux rates were higher following fertilizer application in the P. densiflora and Q. variabilis stands (P. densiflora: 2.180 μmol m^-2 s^-1; Q. variabilis: 1.977 μmol m^-2 s^-1) as compared with the rates measured during the calibration period (P. densiflora: 1.620 μmol m^-2 s^-1; Q. variabilis: 1.557 μmol m^-2 s^-1). The mean annual soil CO₂ efflux rates in the unfertilized treatments of both stands were not significantly different between the two-year study periods. The Q₁₀ values of fertilized treatments in Q. variabilis stands were higher in the fertilization period (3.41) than in the calibration period (3.14), whereas the Q₁₀ values in P. densiflora stands did not change between the fertilization and calibration periods. The Q₁₀ values of unfertilized treatments in the Q. variabilis stands were lower during the 2016-2017 period (3.69), than in the 2015-2016 period (3.85), whereas the Q₁₀ values in P. densiflora stands were higher during the 2016-2017 period (3.65), than in the 2015-2016 period (3.15). These results indicate that the increase in soil CO₂ efflux rates in P. densiflora stands could be more sensitive to fertilization compared with the rates in Q. variabilis stands.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.18 no.4
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• pp.234-265
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• 2013

Oceanic current maps in the secondary school science and earth science textbooks have played an important role in piquing students's inquisitiveness and interests in the ocean. Such maps can provide students with important opportunities to learn about oceanic currents relevant to abrupt climate change and global energy balance issues. Nevertheless, serious and diverse errors in these secondary school oceanic current maps have been discovered upon comparison with up-to-date scientific knowledge concerning oceanic currents. This study presents the fundamental methods and strategies for constructing such maps error-free, through the unification of the diverse current maps currently in the textbooks. In order to do so, we analyzed the maps found in 27 different textbooks and compared them with other up-to-date maps found in scientific journals, and developed a mapping technique for extracting digitalized quantitative information on warm and cold currents in the East Sea. We devised analysis items for the current visualization in relation to the branching features of the Tsushima Warm Current (TWC) in the Korea Strait. These analysis items include: its nearshore and offshore branches, the northern limit and distance from the coast of the East Korea Warm Current, outflow features of the TWC near the Tsugaru and Soya Straits and their returning currents, and flow patterns of the Liman Cold Current and the North Korea Cold Current. The first draft of the current map was constructed based upon the scientific knowledge and input of oceanographers based on oceanic in-situ measurements, and was corrected with the help of a questionnaire survey to the members of an oceanographic society. In addition, diverse comments have been collected from a special session of the 2013 spring meeting of the Korean Oceanographic Society to assist in the construction of an accurate current map of the East Sea which has been corrected repeatedly through in-depth discussions with oceanographers. Finally, we have obtained constructive comments and evaluations of the interim version of the current map from several well-known ocean current experts and incorporated their input to complete the map's final version. To avoid errors in the production of oceanic current maps in future textbooks, we provide the geolocation information (latitude and longitude) of the currents by digitalizing the map. This study is expected to be the first step towards the completion of an oceanographic current map suitable for secondary school textbooks, and to encourage oceanographers to take more interest in oceanic education.

• Korean Journal of Head & Neck Oncology
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• v.17 no.2
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• pp.162-168
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• 2001

Background: Inverted papilloma(IP) of the nasal cavity and paranasal sinuses is a benign neoplastic condition that can be associated with squamous cell carcinoma (SCC). Several studies have indicated an etiologic role for viruses in the development of inverted papilloma. And it is necessary to find out the significance of a biologic markers such as p53, c-erbB-2 to predict the malignant potential. The purposes of this study are to detect HPV in inverted papilloma of the nasal cavity and paranasal sinus, to examine role of HPV as an etiological agent, to examine the relationship between HPV subtype and malignant transformation of inverted papilloma, and to investigate the relation between expression rate of p53, c-erbB-2 and HPV in recurrent or malignant transformation cases. Material and Methods: Thirty two cases of inverted papilloma(IP) in the nasal cavity and paranasal sinuses were reviewed and classified into 3 groups; simple IP, IP with dysplasia group, IP with squamous cell carcinoma group. Paraffin embedded achival tissue was used in this study. The HPV was detected by in situ hybridzation (ISH) using HPV type 6/11, 16/18, 31/33/35 DNA probes. Expression of p53 and c-erbB-2 was examined by immunohistochemical staining. Results: 1) The HPV was detected in 6(19%) out of 32 cases. 2) The HPV 6/11 was dectected in 4 out of 21 cases of simple IP, HPV 16/18 in 1, HPV 31/33/35 in lout of 8 cases of IP with dysplasia respectively. 3) The positive expression of p53 was 13 cases out of 32 cases; 2 out of 21 cases of simple IP, all of 8 cases of IP with dysplasia and 3 cases of IP with squamous cell carcinoma 4) The positive expression of c-erbB-2 was in 24 out of 32 cases; 16 out of 21 cases of simple IP, 6 out of 8 cases of IP with dysplasia, 2 out of 3 cases of IP with squamous cell ca. 5) The recurrence of IP occurred in lout of 6 cases of positive for HPV, in 4 out of 26 cases negative for HPV. 6) The recurrence of IP occurred only in positive cases for p53. 7) The recurrence of IP occurred in 4(17%) out of 24 cases positive for c-erbB-2, in 1(13%) out of 8 cases negative for c-erbB-2. Conclusion: The p53 expression was associated with Inverted papillomas exhibiting evidence of malignant transformation. Also, there was a correlation between the p53 expression and recurrence.

• Journal of Yeungnam Medical Science
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• v.16 no.2
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• pp.342-346
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• 1999

Background: Delays in breast cancer diagnosis may occur in young women due to a low index of suspicion. The purpose of this study was to compare mammography and breast sonography in detection of breast cancer and to suggest a reasonable guideline for breast cancer screening examination. Materials and Methods: Among 820 patients, 102 patients were under 35 years and 122 patients were above 60 years of age. We reviewed medical records, mammograms and/or ultrasonography of 49 patients under 35 years and 48 patients above 60 years of age with pathologically-proven breast cancer. Pathological reports were as follows Invasive ductal carcinoma(IDC) was present 61.2% of patients in the young age group and ductal carcinoma in situ(DCIS) in 16.3%. IDC was present in 66.6% of the patients in the old age group, and DCIS in 8.33%. We analyzed mammography and ultrasonography to evaluate their usefulness in detecting breast cancer in patients under 35 years and over 60 years of age. Results: The mammographic results are as follows : 1) detection rate of lesion: 83.8%(under 35yrs), 100%(over 60yrs) 2) sensitivity of cancer: 67.6%(under 35yrs), 91.2%(over 60yrs) The ultrasonographic results are as follows : 1) detection rate of lesion: 100% 2) sensitivity of cancer: 87.2%(under 35yrs), 96.7%(over 60yrs) The breast cancer detection rate in women under 35 years old was comparable to that of women above 60 years old in our study. Conclusion: A striking histologic finding in the two groups was a higher incidence of nuclear Grade II and III tumors. This finding correlates with the reported increased incidence of high grade tumors in young women and may correlate with the poorer prognosis of breast cancer in young patients. We conclude that early screening examination is helpful for early detection of breast cancer in women under age 35.

• Korean Journal of Ecology and Environment
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• v.43 no.1
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• pp.136-141
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• 2010

In situ experiments of Nutrient Enrichment Bioassays (NEBs) were conducted in the field along with in the laboratory to determine which nutrient limited phytoplankton growth as a indicator of primary productivity. For the NEBs, the water was sampled using a polyethylene-lined container and dispensed into 6 L water tank in the laboratory. The control (C, no nutrient spike) and six treatments of phosphorus (P), 2-fold phosphorus (2P), 4-fold phosphorus (4P), nitrate nitrogen ($NO_3$-N), 2-fold nitrate nitrogen ($2NO_3$-N), and phosphorus and nitrate nitrogen (P+$NO_3$-N) were set up in the lacustrine zone near the dam site, Daechung Reservoir in September, 2009 and analyzed the diel changes of total nitrogen (TN), total phosphorus (TP), and chlorophyll-$\alpha$ (Chl-$\alpha$) in the cubitainers. The short-term NEBs showed that algal response in the treatments spiked phosphorus (P, 2P, and 4P) were significantly (p < 0.05) greater than the response in the control (C), and nitrogen-spike. Also, the response in 4P-treatment was greater than those in the P- and 2P-treatments. In contrast, there was no significant differences (p > 0.20) between the $NO_3$-N and $2NO_3$-N treatment. The outcomes of the NEBs suggest that phosphorus limited the phytoplankton growth and nitrogen was not limited in this system. Furthermore, in the N + P treatments, the response was minimum, compared to all other treatments and the control, indicating that even if the system is evidently P-limited system, when added the nitrogen, the response showed the inhibition. Also, > 95% of observed long-term TN:TP ratios in the ambient water showed > 17, which is the criteria of P-limitation, supporting the P-limitation in the system. Overall, these results suggest that phytoplankton biomass near the dam is a direct linear function of P-loading near the watershed, if the phosphorus pool is mainly dissolved fraction.

• Korean Journal of Environment and Ecology
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• v.35 no.5
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• pp.548-557
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• 2021

Cypripedium japonicum Thunb. is an endemic plant in East Asia, distributed only in Korea, China, and Japan. At the global level, the IUCN Red List evaluates it as "Endangered Species (EN)," and at the national level in Korea, it is evaluated as "Critically Endangered Species (CR)." In this study, we investigated the characteristics of the age structure and the sustainability of the population based on the data obtained by demographic monitoring conducted for seven years in the natural habitat. C. japonicum habitats were observed in 7 regions of Korea (Pochoen, Gapyeong, Hwacheon, Chuncheon, Yeongdong, Muju, Gwangyang), and 4,356 individuals in 15 subpopulations were identified. The population size and structure differed from region to region, and artificial management had a very important effect on the size and structural change of the population. Population viability analysis (PVA) based on changes in the number of individuals of C. japonicum showed a very diverse tendency by region. And the probability of population extinction in the next 100 years was 0.00% for Pocheon, 10.90% for Gwangyang, 24.05% for Chuncheon, and 79.50% for Hwacheon. Since the above monitored study sites were located within the conservation shelters, which restricted access by humans, unauthorized collection of C. japonicum, the biggest threat to the species, was not reflected in the individual viability. So, the risk of extinction in Korea is expected to be significantly higher than that estimated in this study. Therefore, it is necessary to reflect population information in several regions that may represent various threats to determine the extinction risk of the C. japonicum population objectively. In the future, we should expand the demographic monitoring of the C. japonicum population known in Korea.

• Journal of Korean Society of Forest Science
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• v.86 no.2
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• pp.186-199
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• 1997

The soils derived from granite gneiss occupy almost one third of the land area in Korea. The soils under forest vegetation, formed on granite gneiss, in Sun chon-shi, Chollanam-do in southern part of Korea, were studied to evaluate the weathering and the transformation of primary minerals into secondary minerals, clay minerals. The studied soils contained large amounts of ferromagnesian minerals, weathered biotites and were well weathered, strongly acid and low in organic matters and in ration exchange capacity. The clay contents in the Bt horizon were almost two times higher than those in the C horizon. The O horizon had a thin layer which consisted of a little decomposed plant components with a granic fabric and high porosity, and showed the micromorphological characteristics of moder humus. The related distribution pattern of the E horizon were enaulic and large amounts of silts and small amounts of sand grains were another characteristics of the E horizon. The most striking micromorphological features were multilaminated clay coating and infillings in the voids in the Bt and C horizons, and generally limpid ferriargillans ejected from the biotites and imparted red color to the soils in the Bt horizon. High clay contents in the Bt horizon was not only due to clay translocation, but also due to intensive in situ mineral weathering in this horizon. The most significant pedogenic process, revealed by the petrographic microscope and SEM, was the formation of iron oxides from biotites, the formation of tubular halloysites and the weathering models of biotites; wedge weathering and layer weathering. The thick coating on the weathering biotites showed the characteristics of the weathering process and the synthetic hematites were revealed in clays by TEM. Total chemical analysis of clays revealed extensive loss of Ca, and Na and the concentration of Fe and Al. Mineralogical studies of clays by XRD showed that micas were almost completely weathered to kaolinite, vermiculite-kaolinite intergrade, hematite, gibbsite, while halloysites from other primary minerals. Some dioctahedral mica appeared to be resistant in the soils. Parent rock of the soils contained a considerable amounts of biotites and this forest soils showed especially a dominant characteristics of biotite weathering.